##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049430_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12377698 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861153180502544 33.0 31.0 34.0 30.0 34.0 2 31.951902284253503 33.0 31.0 34.0 30.0 34.0 3 31.974058665835926 33.0 31.0 34.0 30.0 34.0 4 35.69606400156152 37.0 35.0 37.0 33.0 37.0 5 35.758321539271684 37.0 35.0 37.0 35.0 37.0 6 35.82049150011577 37.0 35.0 37.0 35.0 37.0 7 35.78614302918039 37.0 35.0 37.0 33.0 37.0 8 35.77765340534241 37.0 35.0 37.0 33.0 37.0 9 37.46867171908702 39.0 37.0 39.0 34.0 39.0 10-11 37.44478799692803 39.0 37.0 39.0 34.5 39.0 12-13 37.42060167407542 39.0 37.0 39.0 34.0 39.0 14-15 38.70628965095125 40.0 38.0 41.0 34.0 41.0 16-17 38.71110811558013 40.0 38.0 41.0 34.0 41.0 18-19 38.76591717619867 40.0 38.0 41.0 35.0 41.0 20-21 38.780299454712825 40.0 38.0 41.0 34.5 41.0 22-23 38.75231832284162 40.0 38.0 41.0 34.0 41.0 24-25 38.734820359973234 40.0 38.0 41.0 34.0 41.0 26-27 38.628502448516684 40.0 38.0 41.0 34.0 41.0 28-29 38.55154561050043 40.0 38.0 41.0 34.0 41.0 30-31 38.4624865625256 40.0 38.0 41.0 34.0 41.0 32-33 38.35455635611727 40.0 38.0 41.0 34.0 41.0 34-35 38.25970596471169 40.0 38.0 41.0 34.0 41.0 36-37 38.158094905854064 40.0 38.0 41.0 34.0 41.0 38-39 38.075591761893044 40.0 38.0 41.0 33.0 41.0 40-41 37.99211751652044 40.0 37.0 41.0 33.0 41.0 42-43 37.86776454717186 40.0 37.0 41.0 33.0 41.0 44-45 37.707045728535306 40.0 37.0 41.0 33.0 41.0 46-47 37.496098587960375 40.0 37.0 41.0 33.0 41.0 48-49 37.28385564908758 40.0 37.0 41.0 32.0 41.0 50-51 37.121201212050906 40.0 36.0 41.0 32.0 41.0 52-53 36.9791632902984 39.5 36.0 41.0 31.0 41.0 54-55 36.81465665101863 39.0 35.5 41.0 31.0 41.0 56-57 36.63640157483241 39.0 35.0 41.0 31.0 41.0 58-59 36.41836297023889 39.0 35.0 41.0 31.0 41.0 60-61 36.198204019842784 38.5 35.0 40.5 31.0 41.0 62-63 35.95195152604305 38.0 35.0 40.0 30.0 41.0 64-65 35.70327047080968 37.5 35.0 40.0 30.0 41.0 66-67 35.41093897265873 37.0 35.0 40.0 30.0 41.0 68-69 35.09674654366265 36.5 34.0 39.5 29.5 41.0 70-71 34.78720732239549 36.0 34.0 39.0 29.0 41.0 72-73 34.45785989446503 35.5 34.0 39.0 29.0 40.5 74-75 34.14630087113129 35.0 34.0 37.5 29.0 40.0 76-77 33.84038397123601 35.0 34.0 37.0 29.0 39.0 78-79 33.54639109792467 35.0 34.0 37.0 29.0 39.0 80-81 33.26638543774456 35.0 34.0 36.0 29.0 39.0 82-83 33.02101388319541 35.0 33.0 36.0 29.0 37.5 84-85 32.77876330477606 35.0 33.0 35.5 28.0 37.0 86-87 32.57097030481758 35.0 33.0 35.0 27.5 37.0 88-89 32.38077734648236 35.0 33.0 35.0 27.0 36.0 90-91 32.21175427773403 35.0 33.0 35.0 27.0 36.0 92-93 32.05846333462006 35.0 33.0 35.0 27.0 36.0 94-95 31.908683100847995 35.0 33.0 35.0 27.0 35.5 96-97 31.74633187043342 35.0 33.0 35.0 26.0 35.0 98-99 31.603536780425568 35.0 33.0 35.0 26.0 35.0 100 31.490988469746153 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2489241684460417 1101 2 0.2692888004457821 1101 3 0.39624948183639574 1101 4 0.25775015472623863 1101 5 0.242225298460653 1101 6 0.20175537471301652 1101 7 0.16856909181579027 1101 8 0.20073431247531204 1101 9 0.28504513092605777 1101 10-11 0.2858227423167108 1101 12-13 0.24724381731117973 1101 14-15 0.3582419935764065 1101 16-17 0.30426804001177743 1101 18-19 0.34830250803622675 1101 20-21 0.3559436027899281 1101 22-23 0.34114324826190057 1101 24-25 0.36532627866417045 1101 26-27 0.3739112878715787 1101 28-29 0.38188433633882113 1101 30-31 0.41367300641304183 1101 32-33 0.4030607973460363 1101 34-35 0.38316144942509567 1101 36-37 0.4235040181640315 1101 38-39 0.4187990407962019 1101 40-41 0.42045134990259925 1101 42-43 0.42409080019200474 1101 44-45 0.42023034033183393 1101 46-47 0.3972557561191863 1101 48-49 0.41485512318114104 1101 50-51 0.3591723362017021 1101 52-53 0.3918283308647972 1101 54-55 0.39075587602339823 1101 56-57 0.39777929901163134 1101 58-59 0.3918228328818856 1101 60-61 0.36896363584179426 1101 62-63 0.40122123945221944 1101 64-65 0.3626414528762538 1101 66-67 0.37910875831365587 1101 68-69 0.3791576093282103 1101 70-71 0.36903425132864953 1101 72-73 0.36763050457071245 1101 74-75 0.3459445767344178 1101 76-77 0.36469175539942 1101 78-79 0.3417861656313761 1101 80-81 0.3789228244805969 1101 82-83 0.3485195889473971 1101 84-85 0.36187064973915994 1101 86-87 0.3043608991371727 1101 88-89 0.3753607466677309 1101 90-91 0.3729977673845539 1101 92-93 0.3959921176383361 1101 94-95 0.3541662910672656 1101 96-97 0.3810119873955955 1101 98-99 0.41077964115643084 1101 100 0.39770777559585113 1102 1 0.22255618217051776 1102 2 0.23189473953113904 1102 3 0.32529889096381126 1102 4 0.2219133333240748 1102 5 0.1945812697794409 1102 6 0.17573149218657136 1102 7 0.1637070294066305 1102 8 0.17280187898517596 1102 9 0.19296243881878183 1102 10-11 0.2526003394107761 1102 12-13 0.24186872416817806 1102 14-15 0.296362465471411 1102 16-17 0.27776596453281854 1102 18-19 0.30563447800625454 1102 20-21 0.28835762015874167 1102 22-23 0.23926098495536507 1102 24-25 0.26496253384362234 1102 26-27 0.2562654823105106 1102 28-29 0.2759634249010432 1102 30-31 0.26715136164029474 1102 32-33 0.2955181795370976 1102 34-35 0.29793693246386965 1102 36-37 0.3160790834714007 1102 38-39 0.26949612271524614 1102 40-41 0.32985583425214315 1102 42-43 0.3054926682554395 1102 44-45 0.2933331364266678 1102 46-47 0.2847379279494433 1102 48-49 0.26081379619282075 1102 50-51 0.2566407810015008 1102 52-53 0.2684805427459409 1102 54-55 0.22955916011724753 1102 56-57 0.2782547694683615 1102 58-59 0.3171253262039997 1102 60-61 0.2844487848421977 1102 62-63 0.2607627572866562 1102 64-65 0.2516333073546164 1102 66-67 0.250785455494011 1102 68-69 0.27787265065904876 1102 70-71 0.2715227592180298 1102 72-73 0.30813821929992713 1102 74-75 0.2652544511293513 1102 76-77 0.238493078279852 1102 78-79 0.27730594093839755 1102 80-81 0.26658263606707067 1102 82-83 0.2574740392767225 1102 84-85 0.2501006214973458 1102 86-87 0.2538168912495351 1102 88-89 0.2618957060320568 1102 90-91 0.24773329094930574 1102 92-93 0.28769973749064803 1102 94-95 0.23694159891265087 1102 96-97 0.2540645557516541 1102 98-99 0.2898362969015835 1102 100 0.3467854464991831 1103 1 0.2080144668570334 1103 2 0.2533052293688023 1103 3 0.35935514687138337 1103 4 0.21960504077448917 1103 5 0.23664923101612345 1103 6 0.19408579709534024 1103 7 0.21416081775618778 1103 8 0.18877941930601594 1103 9 0.21848746924408147 1103 10-11 0.2157548886176741 1103 12-13 0.20258388773796554 1103 14-15 0.3211479079912962 1103 16-17 0.29079944884809805 1103 18-19 0.28941447604540116 1103 20-21 0.2865257563225967 1103 22-23 0.300604472590166 1103 24-25 0.27117154973463187 1103 26-27 0.27289208291126954 1103 28-29 0.3184740154008452 1103 30-31 0.3068729908430754 1103 32-33 0.30331794921561794 1103 34-35 0.29771238464529404 1103 36-37 0.29183075595700103 1103 38-39 0.295072790697418 1103 40-41 0.31284682833616984 1103 42-43 0.335760076824954 1103 44-45 0.35432894771770407 1103 46-47 0.3417199284574082 1103 48-49 0.3035639260599652 1103 50-51 0.29511181926564944 1103 52-53 0.2698082130563648 1103 54-55 0.27245644751683784 1103 56-57 0.25922541473999416 1103 58-59 0.24689576960362558 1103 60-61 0.17770482712422364 1103 62-63 0.21794678929285993 1103 64-65 0.2085277975561013 1103 66-67 0.2115950242583935 1103 68-69 0.21498471131009467 1103 70-71 0.19224458666494115 1103 72-73 0.21341447604068975 1103 74-75 0.208168930642465 1103 76-77 0.18860512504445381 1103 78-79 0.21147049813981766 1103 80-81 0.17941809210769577 1103 82-83 0.16773330736759817 1103 84-85 0.1789235139563914 1103 86-87 0.14551919627184162 1103 88-89 0.17094276741302394 1103 90-91 0.20362483429493494 1103 92-93 0.2254183114847379 1103 94-95 0.18106495529133326 1103 96-97 0.22955771826187998 1103 98-99 0.268146369170001 1103 100 0.23541010222598047 1104 1 0.19676909859122915 1104 2 0.18983232779565995 1104 3 0.28633366835573426 1104 4 0.17855223035183343 1104 5 0.16858441060029605 1104 6 0.1408061734286079 1104 7 0.17343195537849 1104 8 0.1380690481403164 1104 9 0.18751342254457626 1104 10-11 0.2012558149413195 1104 12-13 0.1857669279718337 1104 14-15 0.25308803801033974 1104 16-17 0.2546396105183817 1104 18-19 0.21495902001508682 1104 20-21 0.2595991502235222 1104 22-23 0.24932592264661935 1104 24-25 0.25230517333881863 1104 26-27 0.28417568443259 1104 28-29 0.2549086345698228 1104 30-31 0.2794386442650634 1104 32-33 0.2748007456579771 1104 34-35 0.2426802699169457 1104 36-37 0.2479911167037443 1104 38-39 0.2444591331822039 1104 40-41 0.26636771030972994 1104 42-43 0.3001378950042266 1104 44-45 0.3107971092146542 1104 46-47 0.2695129287064333 1104 48-49 0.2593693355227913 1104 50-51 0.29391811182666316 1104 52-53 0.2666920542647233 1104 54-55 0.2830493729015515 1104 56-57 0.29850234905708106 1104 58-59 0.3249001337808082 1104 60-61 0.3325560785418844 1104 62-63 0.31183947908265 1104 64-65 0.2317385416669353 1104 66-67 0.26249616579062973 1104 68-69 0.2927156138360729 1104 70-71 0.30330978910401996 1104 72-73 0.34563740719116964 1104 74-75 0.34750848231586673 1104 76-77 0.268559931177478 1104 78-79 0.27978606016975505 1104 80-81 0.2767890663146204 1104 82-83 0.28076884629365395 1104 84-85 0.25490274353386866 1104 86-87 0.24388172974759215 1104 88-89 0.25846764214970364 1104 90-91 0.24438317812842314 1104 92-93 0.2788492953549806 1104 94-95 0.284607032425761 1104 96-97 0.2862907952984699 1104 98-99 0.3464436796997248 1104 100 0.3189906773814535 1105 1 0.15810169904899496 1105 2 0.14086523853359623 1105 3 0.25683977251933143 1105 4 0.16305105309699996 1105 5 0.13211489652955066 1105 6 0.1235622811735837 1105 7 0.12588199181140425 1105 8 0.11278284151192253 1105 9 0.16682447354234142 1105 10-11 0.13438738871036549 1105 12-13 0.13105324052472866 1105 14-15 0.2025745766373106 1105 16-17 0.20751160692491055 1105 18-19 0.16986080802685422 1105 20-21 0.1914403624817993 1105 22-23 0.14220385363444166 1105 24-25 0.16520119171143932 1105 26-27 0.17233346116923798 1105 28-29 0.18532887150695387 1105 30-31 0.17149014450397715 1105 32-33 0.17814123125597092 1105 34-35 0.19621172103236262 1105 36-37 0.1838786282723106 1105 38-39 0.21730081868447115 1105 40-41 0.24473522182258733 1105 42-43 0.20788485159305026 1105 44-45 0.19771147911978204 1105 46-47 0.2041272481566594 1105 48-49 0.2351109641333835 1105 50-51 0.2079450624914898 1105 52-53 0.19682827218031917 1105 54-55 0.20455172519498177 1105 56-57 0.17572445721021523 1105 58-59 0.1721064183863632 1105 60-61 0.1309960118640916 1105 62-63 0.17238215837098636 1105 64-65 0.1609658831716203 1105 66-67 0.17187509337880869 1105 68-69 0.17872615697579164 1105 70-71 0.19799157032353065 1105 72-73 0.22641771762544494 1105 74-75 0.23859848761247093 1105 76-77 0.20196803244014916 1105 78-79 0.20349768691019676 1105 80-81 0.20933303260949288 1105 82-83 0.20003922240448446 1105 84-85 0.1765160385860156 1105 86-87 0.15164425819376248 1105 88-89 0.1388962946146961 1105 90-91 0.18429948038149035 1105 92-93 0.21097909358259415 1105 94-95 0.16024602217231632 1105 96-97 0.13809116338191174 1105 98-99 0.18659203191790752 1105 100 0.17819740936856476 1106 1 0.09646949711896013 1106 2 0.06829360706916177 1106 3 0.1990888942639053 1106 4 0.1087818447825768 1106 5 0.09621262208110437 1106 6 0.0958002153751707 1106 7 0.09195518290412963 1106 8 0.0878938478644713 1106 9 0.0861819971690636 1106 10-11 0.09375937435223136 1106 12-13 0.09689434939266306 1106 14-15 0.1345481078722699 1106 16-17 0.1348600832942921 1106 18-19 0.1255417532548364 1106 20-21 0.1481944659075296 1106 22-23 0.14372475924874095 1106 24-25 0.1252564386317374 1106 26-27 0.1167463011612142 1106 28-29 0.1311779429912434 1106 30-31 0.16171367443938323 1106 32-33 0.11021867611666636 1106 34-35 0.11678022674244204 1106 36-37 0.11504151139326524 1106 38-39 0.10729985457719948 1106 40-41 0.0935670194611049 1106 42-43 0.07530899495084498 1106 44-45 0.06085759952614467 1106 46-47 0.0992374333529753 1106 48-49 0.09315331630170931 1106 50-51 0.08417848220189939 1106 52-53 0.06580000263834052 1106 54-55 0.10979768707189663 1106 56-57 0.11893835630693417 1106 58-59 0.09139572553518605 1106 60-61 0.04052414917636327 1106 62-63 0.013787842607740686 1106 64-65 -0.010406194170080596 1106 66-67 -0.030063418101299533 1106 68-69 -0.022584111099632764 1106 70-71 -0.00291970394849983 1106 72-73 0.030860974917487738 1106 74-75 0.02433704860562358 1106 76-77 -0.02090134328943094 1106 78-79 0.005623736341362928 1106 80-81 -0.020567300466424854 1106 82-83 0.02353793397705317 1106 84-85 -0.019408447691816377 1106 86-87 0.022143412345990043 1106 88-89 0.00791471767308849 1106 90-91 0.0411429119600939 1106 92-93 0.012461071436881355 1106 94-95 -0.032150487369502656 1106 96-97 0.024894482168893006 1106 98-99 0.02685523482683294 1106 100 0.018748402764554584 1107 1 0.1281628679555773 1107 2 0.11526517748148635 1107 3 0.2310114067958544 1107 4 0.1384464655232378 1107 5 0.13741836232600946 1107 6 0.11043238439604863 1107 7 0.11092860729090859 1107 8 0.11161456658751945 1107 9 0.10667768233168573 1107 10-11 0.12361163276277409 1107 12-13 0.10707368836201425 1107 14-15 0.1386701599275355 1107 16-17 0.1268400514354937 1107 18-19 0.15828018171582414 1107 20-21 0.15312659514415117 1107 22-23 0.16687549618117714 1107 24-25 0.1772500729121944 1107 26-27 0.17640147729379407 1107 28-29 0.1885234066693755 1107 30-31 0.1781538246193719 1107 32-33 0.20902475431613965 1107 34-35 0.20416598750606596 1107 36-37 0.2321622479584846 1107 38-39 0.19682908132883625 1107 40-41 0.24981018957676326 1107 42-43 0.25756081114549545 1107 44-45 0.2418544618007772 1107 46-47 0.23644198873878963 1107 48-49 0.26579254879095515 1107 50-51 0.27691339017960104 1107 52-53 0.276010253979905 1107 54-55 0.30772973176186014 1107 56-57 0.3018926480759987 1107 58-59 0.2988370098030302 1107 60-61 0.2986041312941481 1107 62-63 0.3310320526177293 1107 64-65 0.2263786550822644 1107 66-67 0.28827491861883203 1107 68-69 0.2735061440060278 1107 70-71 0.2741769869302999 1107 72-73 0.3361189896040315 1107 74-75 0.3282579189734349 1107 76-77 0.2865930895087416 1107 78-79 0.2975040026756304 1107 80-81 0.3217563913093997 1107 82-83 0.3322712236120253 1107 84-85 0.3166918671238932 1107 86-87 0.25819030702864865 1107 88-89 0.271101981503854 1107 90-91 0.31217970085977953 1107 92-93 0.2999493509970037 1107 94-95 0.3016649645085039 1107 96-97 0.268234395038057 1107 98-99 0.32313943703331915 1107 100 0.3207279833812713 1108 1 0.06364079918794374 1108 2 0.051210793277856936 1108 3 0.1176725199906592 1108 4 0.0657589166678676 1108 5 0.06858511378051446 1108 6 0.07795584962724433 1108 7 0.047452466182605235 1108 8 0.03069544201273544 1108 9 0.08912218217435708 1108 10-11 0.06804929330367315 1108 12-13 0.04639708423773925 1108 14-15 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0.38671136981923837 2201 20-21 0.3794893298042794 2201 22-23 0.40377002773202264 2201 24-25 0.38847020375332164 2201 26-27 0.4197244836748695 2201 28-29 0.4119770885447096 2201 30-31 0.41836723902478923 2201 32-33 0.4316357485817335 2201 34-35 0.44798934532602175 2201 36-37 0.4895850150995855 2201 38-39 0.4985525900508634 2201 40-41 0.47997998291537414 2201 42-43 0.4735251630747541 2201 44-45 0.4860856551266366 2201 46-47 0.4577269847596881 2201 48-49 0.4547431927744938 2201 50-51 0.47815552125336325 2201 52-53 0.483406741961403 2201 54-55 0.44558583882692204 2201 56-57 0.40401270048450755 2201 58-59 0.4159022190829944 2201 60-61 0.4275742437082499 2201 62-63 0.4398090994613142 2201 64-65 0.40698547327554024 2201 66-67 0.42200973618769666 2201 68-69 0.3757388245715134 2201 70-71 0.35556271666571604 2201 72-73 -0.013572906652299821 2201 74-75 0.019260058889081222 2201 76-77 0.05188828564291015 2201 78-79 0.24599102232478742 2201 80-81 0.37296661888071725 2201 82-83 0.362984254040839 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42-43 0.4880427468530115 2202 44-45 0.4967341245198682 2202 46-47 0.5098476417038782 2202 48-49 0.5331842384884027 2202 50-51 0.5432423345262904 2202 52-53 0.5132797204891091 2202 54-55 0.5083698956582126 2202 56-57 0.4693264753879518 2202 58-59 0.49805690145696957 2202 60-61 0.5828984845484584 2202 62-63 0.49652793536137807 2202 64-65 0.5169972825113476 2202 66-67 0.5117809256395205 2202 68-69 0.4863196590271599 2202 70-71 0.3788688704897112 2202 72-73 0.4279160560096713 2202 74-75 0.29455962332905017 2202 76-77 0.3049774157303702 2202 78-79 0.39896853387699593 2202 80-81 0.40605395872194805 2202 82-83 0.4200038101463974 2202 84-85 0.4239578575209606 2202 86-87 0.4298302300238461 2202 88-89 0.4192591840548019 2202 90-91 0.45045552031344727 2202 92-93 0.4954500758700462 2202 94-95 0.5024288252846674 2202 96-97 0.5168926527167983 2202 98-99 0.5106766188175911 2202 100 0.5588382254732736 2203 1 0.31342104401820237 2203 2 0.34687424312629744 2203 3 0.43759640421270873 2203 4 0.23722839907048865 2203 5 0.23150554761317466 2203 6 0.1850448262600466 2203 7 0.2087727197597502 2203 8 0.20304225462585634 2203 9 0.29051127735770166 2203 10-11 0.2526380773949768 2203 12-13 0.22769900740635052 2203 14-15 0.3626880831958843 2203 16-17 0.1328580278003244 2203 18-19 0.21727687102412574 2203 20-21 0.28558904430301624 2203 22-23 0.322157693769185 2203 24-25 0.31973483416135906 2203 26-27 0.32678731710308995 2203 28-29 0.32842812547507094 2203 30-31 0.37683309480022587 2203 32-33 0.35775804236956077 2203 34-35 0.4130825483754279 2203 36-37 0.40091583197823155 2203 38-39 0.41074367519395594 2203 40-41 0.41233829593721794 2203 42-43 0.39631515460723676 2203 44-45 0.39819220663686394 2203 46-47 0.3737713684502708 2203 48-49 0.4325549715621477 2203 50-51 0.419179233469805 2203 52-53 0.42695759657090093 2203 54-55 0.4275274383080543 2203 56-57 0.2455200737085903 2203 58-59 0.3098016103621859 2203 60-61 0.3241651194267092 2203 62-63 0.3972589695105171 2203 64-65 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2214 98-99 -0.28897072005487345 2214 100 -0.2977417708268675 2215 1 -0.36244511848417105 2215 2 -0.40598093750523034 2215 3 -0.3136344241980318 2215 4 -0.3109842610380369 2215 5 -0.31773339438181836 2215 6 -0.2559935451030668 2215 7 -0.2683061875326729 2215 8 -0.27929706217050665 2215 9 -0.4640769003136569 2215 10-11 -0.4291168576294524 2215 12-13 -0.426369357521061 2215 14-15 -0.561665269576892 2215 16-17 -0.5480903756972992 2215 18-19 -0.5210905212002359 2215 20-21 -0.5383924149902413 2215 22-23 -0.5189057779762862 2215 24-25 -0.5275714141652657 2215 26-27 -0.5102054030961014 2215 28-29 -0.5118665049237805 2215 30-31 -0.5724826942780723 2215 32-33 -0.5074119459646269 2215 34-35 -0.5325133195747327 2215 36-37 -0.5482915448102688 2215 38-39 -0.5693484329627125 2215 40-41 -0.6004580082889177 2215 42-43 -0.5528064776601767 2215 44-45 -0.5384236603106203 2215 46-47 -0.5638013231309529 2215 48-49 -0.5146336706259831 2215 50-51 -0.4681262998932638 2215 52-53 -0.511027164944295 2215 54-55 -0.44864308709203726 2215 56-57 -0.4693988315886841 2215 58-59 -0.44946281734935667 2215 60-61 -0.4712231050162501 2215 62-63 -0.4559729672725936 2215 64-65 -0.49417536714069143 2215 66-67 -0.44134903146866833 2215 68-69 -0.4354292791211094 2215 70-71 -0.4095634420812502 2215 72-73 -0.4339618871187483 2215 74-75 -0.40016176148726146 2215 76-77 -0.35766905390369885 2215 78-79 -0.3451435486783083 2215 80-81 -0.42092255022065217 2215 82-83 -0.44861309512743475 2215 84-85 -0.40423521261126893 2215 86-87 -0.3817286094515637 2215 88-89 -0.3881066724705775 2215 90-91 -0.40825047684768734 2215 92-93 -0.38516879956543093 2215 94-95 -0.40175856607005755 2215 96-97 -0.37518111819930056 2215 98-99 -0.36591133911752394 2215 100 -0.3779016435225202 2216 1 -0.26611829179536883 2216 2 -0.2810550050926963 2216 3 -0.2696516455757667 2216 4 -0.197974378526375 2216 5 -0.28560075364473647 2216 6 -0.25100850684457754 2216 7 -0.31175724497889234 2216 8 -0.23973234758388173 2216 9 -0.3808781366233589 2216 10-11 -0.3321314980455554 2216 12-13 -0.3257790726869345 2216 14-15 -0.5353231151372526 2216 16-17 -0.46227219014396326 2216 18-19 -0.4286025558423532 2216 20-21 -0.40250917772731043 2216 22-23 -0.45388998236629163 2216 24-25 -0.4368828906095885 2216 26-27 -0.46676160732678795 2216 28-29 -0.5314882948606012 2216 30-31 -0.5248395884265662 2216 32-33 -0.5042786916581008 2216 34-35 -0.4918392738654802 2216 36-37 -0.5816593272267667 2216 38-39 -0.527384430856884 2216 40-41 -0.5811226872801143 2216 42-43 -0.5966592417109808 2216 44-45 -0.6269200415857981 2216 46-47 -0.6245770145472633 2216 48-49 -0.6140725430173646 2216 50-51 -0.5657637541621412 2216 52-53 -0.5300480754966088 2216 54-55 -0.5109167057774187 2216 56-57 -0.5452011255331328 2216 58-59 -0.5274481824374746 2216 60-61 -0.5449050131430937 2216 62-63 -0.5251264668136102 2216 64-65 -0.5876609538497277 2216 66-67 -0.5638138535090391 2216 68-69 -0.5757676559285017 2216 70-71 -0.60919632975002 2216 72-73 -0.5883841399999383 2216 74-75 -0.5992949023287295 2216 76-77 -0.49795559564351066 2216 78-79 -0.5362991100934806 2216 80-81 -0.6412811782434886 2216 82-83 -0.618764699264581 2216 84-85 -0.7113276302460605 2216 86-87 -0.587533668944225 2216 88-89 -0.6794209571100183 2216 90-91 -0.576889877402003 2216 92-93 -0.5725866798812973 2216 94-95 -0.5888382919145556 2216 96-97 -0.6375921843274561 2216 98-99 -0.6939785242995598 2216 100 -0.6857559521369829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 677.0 3 83.0 4 139.0 5 250.0 6 723.0 7 1898.0 8 4282.0 9 7055.0 10 9058.0 11 11399.0 12 15855.0 13 21330.0 14 23595.0 15 30306.0 16 39967.0 17 56162.0 18 55715.0 19 34305.0 20 28303.0 21 29329.0 22 32007.0 23 35579.0 24 42301.0 25 52809.0 26 69434.0 27 91361.0 28 119726.0 29 158508.0 30 205298.0 31 266150.0 32 343517.0 33 448202.0 34 618361.0 35 911017.0 36 1540762.0 37 2781196.0 38 3279756.0 39 1008902.0 40 2381.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.9730972801006 19.295204112128737 14.114920051285171 23.61677855648549 2 16.17657015060474 20.064441707981565 38.35974993088376 25.39923821052994 3 18.30137558696294 24.882938653051642 29.919335566274118 26.896350193711307 4 13.287721190159916 17.082982635381796 36.719218710942854 32.91007746351543 5 13.891802500393075 36.795307080827364 34.4374067103431 14.875483708436462 6 31.700265833166906 36.6887014763985 17.301946857926943 14.309085832507654 7 28.145637419817483 31.934096307730243 21.44155561074442 18.478710661707858 8 26.42792631612029 34.72883907954693 19.619279434033192 19.22395517029959 9 25.645939980115852 16.30526936430344 20.48583670404626 37.562953951534446 10-11 24.780258009203326 26.195044506660288 27.822043323403108 21.20265416073328 12-13 25.474051381118084 23.550118816608475 28.095882795139353 22.87994700713409 14-15 22.688937098357755 25.31523330570973 25.685443516453688 26.310386079478835 16-17 22.333979226185676 28.62026929401574 26.164158311181936 22.88159316861665 18-19 22.29098794242385 27.284534802319815 28.574027716792184 21.850449538464147 20-21 23.068331941582663 26.354504909475885 28.593326253932883 21.983836895008572 22-23 22.857319672850316 26.18543044110464 28.54772753382737 22.409522352217675 24-25 22.696176096789603 26.359984853397155 28.424905190329085 22.518933859484154 26-27 22.519850322703448 26.44064074770623 28.492981589343508 22.546527340246815 28-29 22.532809342036575 26.372222305032526 28.621317885212232 22.473650467718663 30-31 22.645903139662966 26.556884810083424 28.381622333975187 22.415589716278422 32-33 22.503925711834068 26.44377184550124 28.553466532915284 22.498835909749406 34-35 22.690989719814553 26.28934547183462 28.510728370669984 22.508936437680845 36-37 22.864615630591334 26.357927451034808 28.281666001559014 22.495790916814848 38-39 22.68564172657151 26.504837010730437 28.284790064960447 22.52473119773761 40-41 22.69945931364353 26.39354820023617 28.358655835192558 22.548336650927737 42-43 22.679151288516806 26.4213506552641 28.26329907839764 22.63619897782145 44-45 22.669374011149173 26.58748036840643 28.085320615396004 22.657825005048398 46-47 22.90015006026163 26.529759905197203 27.867234397618812 22.702855636922354 48-49 22.94555311446578 26.607821981983115 27.614594784146952 22.832030119404152 50-51 22.88644998312285 26.740342012341245 27.467049161547376 22.906158842988532 52-53 22.854423213925333 26.810719849302867 27.432747057322725 22.90210987944908 54-55 22.87807696457531 26.944969459748986 27.46007804450292 22.716875531172782 56-57 22.7668257823953 26.78494194206127 27.487965534086218 22.960266741457215 58-59 22.764616848849908 26.48864669334396 27.66008061194923 23.086655845856903 60-61 22.863289976163973 26.6713102358384 27.5335792623366 22.931820525661024 62-63 22.66783759295473 26.73715922995169 27.665068492320643 22.929934684772935 64-65 22.738776937394256 26.564263001747822 27.630294589891708 23.06666547096621 66-67 22.61534509001716 26.573021997731395 27.63230712801471 23.179325784236738 68-69 22.76620879240734 26.688157849876642 27.497844095244695 23.047789262471323 70-71 22.806112760293598 26.51438801361951 27.4909723602114 23.188526865875485 72-73 22.630061315295247 26.65429464789069 27.526200020650105 23.189444016163957 74-75 22.668032568352984 26.664672482520928 27.410718180980897 23.25657676814519 76-77 22.711135346509668 26.66132128820201 27.30715363605777 23.320389729230552 78-79 22.746971322406917 26.680486304424168 27.39188687136164 23.180655501807284 80-81 22.59297222200678 26.63565653608205 27.41881927047381 23.35255197143736 82-83 22.576506307911867 26.6161172483449 27.486383557446015 23.320992886297216 84-85 22.618615577172474 26.57533624591882 27.292671375623023 23.51337680128568 86-87 22.649466361012603 26.67267455803966 27.277880272438715 23.399978808509026 88-89 22.483961910117216 26.595537975361065 27.375505602273144 23.54499451224857 90-91 22.42631754456516 26.530325434674022 27.37103487390146 23.672322146859358 92-93 22.481720858735983 26.6261226107931 27.3651654168314 23.52699111363952 94-95 22.4187443794242 26.37391291772426 27.46011658400197 23.74722611884956 96-97 22.40687795988005 26.50498494951427 27.35918358360922 23.72895350699646 98-99 22.265607042729965 26.551792922513346 27.357910617134856 23.824689417621837 100 22.353971593583456 26.37460527568049 27.29625212459966 23.975171006136396 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2214.0 1 1659.5 2 4651.5 3 6855.5 4 3474.5 5 1538.5 6 2915.5 7 7077.5 8 12704.0 9 14924.0 10 10853.5 11 6327.5 12 5527.5 13 6048.5 14 6843.0 15 7709.5 16 8498.0 17 9528.0 18 11000.0 19 12392.0 20 14200.0 21 17054.5 22 21208.5 23 27942.0 24 37066.5 25 48553.0 26 64380.0 27 84174.5 28 107709.0 29 133087.5 30 155493.5 31 177299.5 32 200147.0 33 220132.5 34 239523.5 35 261717.5 36 284770.5 37 308560.5 38 333571.0 39 353308.5 40 371136.5 41 389316.5 42 400313.5 43 406991.5 44 413785.0 45 420149.5 46 426026.5 47 424354.5 48 419577.5 49 421651.0 50 423954.0 51 420172.0 52 407981.0 53 399358.5 54 392154.5 55 374205.0 56 349936.5 57 320911.5 58 292892.5 59 272424.5 60 249752.5 61 218864.5 62 188299.0 63 159994.0 64 132445.5 65 109275.0 66 87332.0 67 66916.0 68 50695.0 69 37490.5 70 27409.5 71 19774.5 72 14543.0 73 10654.5 74 8124.5 75 6072.0 76 3987.5 77 2695.5 78 1936.5 79 1201.5 80 645.0 81 315.5 82 152.5 83 76.0 84 47.5 85 32.5 86 32.0 87 28.5 88 21.0 89 17.0 90 10.5 91 4.0 92 4.5 93 4.0 94 1.0 95 1.5 96 2.0 97 1.5 98 1.0 99 0.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0870113328019475 2 0.0 3 0.0 4 0.0 5 0.007465039137325859 6 8.079046685417595E-5 7 0.0 8 8.886951353959355E-5 9 0.0 10-11 0.0 12-13 0.013673786515069281 14-15 4.039523342708798E-6 16-17 0.0 18-19 2.4237140056252788E-5 20-21 1.6158093370835193E-5 22-23 0.0 24-25 4.039523342708798E-5 26-27 2.019761671354399E-5 28-29 9.290903688230235E-5 30-31 0.0 32-33 5.251380345521437E-5 34-35 2.4237140056252788E-5 36-37 2.019761671354399E-5 38-39 9.694856022501115E-5 40-41 3.635571008437918E-5 42-43 1.3734379365209912E-4 44-45 7.675094351146715E-5 46-47 3.2316186741670386E-5 48-49 2.3833187721981905E-4 50-51 1.2926474696668154E-4 52-53 1.938971204500223E-4 54-55 1.3330427030939035E-4 56-57 1.575414103656431E-4 58-59 3.635571008437918E-5 60-61 5.251380345521437E-5 62-63 2.666085406187807E-4 64-65 1.2926474696668154E-4 66-67 4.685847077542205E-4 68-69 5.170589878667262E-4 70-71 1.3330427030939035E-4 72-73 2.9084568067503345E-4 74-75 3.070037740458686E-4 76-77 2.221737838489839E-4 78-79 0.0011512641526720073 80-81 0.0010502760691042876 82-83 8.684975186823915E-4 84-85 0.0012118570028126394 86-87 7.149956316594572E-4 88-89 0.0010745132091605403 90-91 0.001417872693290788 92-93 0.001078552732503249 94-95 0.0014946236368022553 96-97 0.001539058393572052 98-99 0.001817785504218959 100 5.816913613500669E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2377698E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.12591214172061 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55223196966013 34.43516429315876 2 15.512779544270819 14.931333308429162 3 5.058701371311362 7.303638532407966 4 2.3990384866493364 4.618236617323693 5 1.3130168310158987 3.159506632503578 6 0.8640736909320937 2.4950600720262117 7 0.586524600878071 1.9758921987571054 8 0.4185784223552075 1.6115574702949393 9 0.30811350410949484 1.3345419085606098 >10 1.780580502716396 15.700887109513396 >50 0.13406649455892689 4.445561146884889 >100 0.06889797116473044 5.876752052237179 >500 0.002689488052505286 0.8475977832080466 >1k 6.061068792566387E-4 0.47417011578325485 >5k 3.367181520424667E-5 0.10032433903683782 >10k+ 6.734363040849333E-5 0.68977641987434 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31362 0.25337506214806665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27667 0.2235229846454486 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15779 0.12747927764920425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.079046685417595E-6 0.0 0.0 0.0 3 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 4 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 5 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 6 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 7 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 8 0.0 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 9 8.079046685417595E-6 1.615809337083519E-5 0.0 8.079046685417595E-6 0.0 10-11 8.079046685417595E-6 2.4237140056252788E-5 0.0 3.635571008437918E-5 0.0 12-13 8.079046685417595E-6 2.4237140056252788E-5 0.0 4.039523342708798E-5 0.0 14-15 8.079046685417595E-6 4.443475676979677E-5 0.0 5.655332679792317E-5 0.0 16-17 8.079046685417595E-6 6.463237348334076E-5 0.0 6.463237348334076E-5 0.0 18-19 8.079046685417595E-6 8.079046685417595E-5 0.0 8.079046685417595E-5 0.0 20-21 8.079046685417595E-6 8.482999019688474E-5 0.0 1.4542284033751673E-4 0.0 22-23 8.079046685417595E-6 1.0098808356771995E-4 0.0 3.110432973885774E-4 0.0 24-25 1.615809337083519E-5 1.2118570028126392E-4 0.0 6.261261181198636E-4 0.0 26-27 1.615809337083519E-5 1.2118570028126392E-4 0.0 0.0011431851059865899 0.0 28-29 1.615809337083519E-5 1.2926474696668152E-4 0.0 0.002880180143351373 0.0 30-31 1.615809337083519E-5 1.2926474696668152E-4 0.0 0.007162074886622698 0.0 32-33 1.615809337083519E-5 1.3734379365209912E-4 0.0 0.01623080479100395 0.0 34-35 1.615809337083519E-5 1.4542284033751673E-4 0.0 0.030385294583855575 0.0 36-37 1.615809337083519E-5 1.5754141036564313E-4 0.0 0.05466686939687816 0.0 38-39 1.615809337083519E-5 1.6965998039376951E-4 0.0 0.08972993201159052 0.0 40-41 2.4237140056252784E-5 1.6965998039376951E-4 0.0 0.12837201230794287 0.0 42-43 3.231618674167038E-5 1.6965998039376951E-4 0.0 0.16876320621168817 0.0 44-45 3.231618674167038E-5 1.736995037364783E-4 0.0 0.21738694868787395 0.0 46-47 3.231618674167038E-5 1.898575971073135E-4 0.0 0.27895736347744143 0.0 48-49 3.231618674167038E-5 2.0197616713543988E-4 0.0 0.3467324861214096 0.0 50-51 3.231618674167038E-5 2.2621330719169267E-4 0.0 0.4171777336949084 0.0 52-53 3.231618674167038E-5 2.3025283053440147E-4 0.0 0.4892509091755187 0.0 54-55 3.231618674167038E-5 2.3833187721981907E-4 0.0 0.5699363484227843 0.0 56-57 3.635571008437918E-5 2.4237140056252785E-4 0.0 0.6664930748835527 0.0 58-59 4.039523342708798E-5 2.5852949393336303E-4 0.0 0.773649510595589 0.0 60-61 4.039523342708798E-5 2.5852949393336303E-4 0.0 0.8868611917983458 0.0 62-63 4.8474280112505575E-5 2.5852949393336303E-4 0.0 1.0023875198764747 0.0 64-65 4.8474280112505575E-5 2.7468758730419824E-4 0.0 1.12466389146027 0.0 66-67 4.8474280112505575E-5 2.8276663398961585E-4 0.0 1.2636356130194808 0.0 68-69 4.8474280112505575E-5 2.8276663398961585E-4 0.0 1.4219445328202385 0.0 70-71 5.251380345521437E-5 2.9488520401774225E-4 0.0 1.5926022754796572 0.0 72-73 5.655332679792317E-5 2.9892472736045106E-4 0.0 1.7719288352325289 0.0 74-75 5.655332679792317E-5 2.9892472736045106E-4 0.0 1.9546889898267028 0.0 76-77 5.655332679792317E-5 2.9892472736045106E-4 0.0 2.1518500451376337 0.0 78-79 6.463237348334076E-5 3.029642507031598E-4 0.0 2.3723231896593373 0.0 80-81 6.867189682604956E-5 3.070037740458686E-4 0.0 2.6098673598273283 0.0 82-83 7.271142016875836E-5 3.070037740458686E-4 0.0 2.857356836465068 0.0 84-85 7.271142016875836E-5 3.070037740458686E-4 0.0 3.106397490066408 0.0 86-87 7.271142016875836E-5 3.070037740458686E-4 0.0 3.3706792652397883 0.0 88 7.271142016875836E-5 3.070037740458686E-4 0.0 3.585852555135858 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19380 0.0 49.92803 1 GTATCAA 32785 0.0 39.31328 1 TCAACGC 39855 0.0 31.747305 4 ATCAACG 40030 0.0 31.561546 3 CAACGCA 41870 0.0 30.320494 5 TATCAAC 42845 0.0 29.696327 2 AACGCAG 43415 0.0 29.410292 6 TATAACG 1335 0.0 25.701365 2 ACGCAGA 50000 0.0 25.480541 7 CGCAGAG 51595 0.0 24.701954 8 ATAACGC 1680 0.0 23.221134 3 GCAGAGT 57240 0.0 22.14265 9 GTACATG 40325 0.0 22.058758 1 GTGGTAT 9570 0.0 22.020643 1 TACCTGG 10095 0.0 21.929525 2 TACATGG 40355 0.0 21.13946 2 GTACCTG 11090 0.0 20.699133 1 GAGTACT 35645 0.0 20.22885 12-13 CAGAGTA 54730 0.0 20.117353 10-11 AGAGTAC 52590 0.0 20.006277 10-11 >>END_MODULE