FastQCFastQC Report
Wed 25 May 2016
SRR2049429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15295276
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670400.43830526497200833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564090.3688001445675122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323890.21175819252951042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA320550.059.69131
GTATCAA620500.043.590621
GTGGTAT121550.034.508261
ATCAACG778750.034.267733
TCAACGC785000.034.0080074
TATCAAC807400.033.14942
CAACGCA816950.032.7063145
TGGTATC126300.031.8906232
AACGCAG864350.031.115976
TATAACG14850.028.146652
CGCAGAG968400.027.4208858
ACGCAGA976650.027.30137
GTACATG636400.025.1993641
GCAGAGT1030150.025.0393929
TACATGG621700.024.6769892
GTACCTG113150.023.9717031
TACCTGG113100.023.0977952
ACATGGG644950.022.8611153
GAGTACT676000.021.64684912-13
CAGAGTA1010700.021.41755310-11
Produced by FastQC (version 0.11.5)