##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15295276 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501578853496987 31.0 31.0 34.0 30.0 34.0 2 31.539020086986334 31.0 31.0 34.0 30.0 34.0 3 31.554878839714956 33.0 31.0 34.0 28.0 34.0 4 35.20191822625495 37.0 35.0 37.0 33.0 37.0 5 35.22976682473726 37.0 35.0 37.0 33.0 37.0 6 35.33124593501941 37.0 35.0 37.0 33.0 37.0 7 35.28043312196524 37.0 35.0 37.0 33.0 37.0 8 35.25634633856885 37.0 35.0 37.0 33.0 37.0 9 36.8431962260766 39.0 37.0 39.0 33.0 39.0 10-11 36.77266065679364 39.0 37.0 39.0 33.0 39.0 12-13 36.73635977539732 39.0 37.0 39.0 33.0 39.0 14-15 37.89265339180542 40.0 38.0 41.0 33.0 41.0 16-17 37.87687476185457 40.0 38.0 41.0 33.0 41.0 18-19 37.91227291354534 40.0 38.0 41.0 33.0 41.0 20-21 37.88006100707173 40.0 38.0 41.0 33.0 41.0 22-23 37.76687998307451 40.0 38.0 41.0 33.0 41.0 24-25 37.65235573388803 40.0 38.0 41.0 32.5 41.0 26-27 37.50626781759283 40.0 37.0 41.0 32.0 41.0 28-29 37.34818142542835 40.0 37.0 41.0 32.0 41.0 30-31 37.14706207328328 40.0 37.0 41.0 32.0 41.0 32-33 36.87791410236729 39.5 36.0 41.0 31.0 41.0 34-35 36.66916422430036 39.0 36.0 41.0 30.5 41.0 36-37 36.47194359225685 39.0 36.0 41.0 30.0 41.0 38-39 36.256314400603166 39.0 35.0 40.0 30.0 41.0 40-41 36.03232099244237 39.0 35.0 40.0 30.0 41.0 42-43 35.7869133580852 39.0 35.0 40.0 29.0 41.0 44-45 35.488525869033026 38.5 35.0 40.0 27.5 41.0 46-47 35.09990061637332 38.0 35.0 40.0 26.5 41.0 48-49 34.78075155361695 38.0 34.0 40.0 24.5 41.0 50-51 33.42297667593576 36.5 32.5 39.0 23.0 40.0 52-53 33.73621155969987 37.5 33.5 39.0 23.5 40.0 54-55 34.78144510108873 38.5 35.0 40.0 26.0 41.0 56-57 34.9021303701875 39.0 35.0 40.0 26.0 41.0 58-59 34.74499551364748 38.5 34.5 40.0 25.5 41.0 60-61 34.48305571602631 38.0 34.0 40.0 24.5 41.0 62-63 34.20909625952484 38.0 34.0 40.0 23.5 41.0 64-65 33.92685522641108 37.0 34.0 40.0 23.0 41.0 66-67 33.5987680771501 37.0 33.5 40.0 22.0 41.0 68-69 33.24388056155378 36.0 33.0 39.0 21.5 41.0 70-71 32.89744241947645 36.0 33.0 39.0 20.0 41.0 72-73 32.626615073830635 35.0 33.0 39.0 20.0 40.0 74-75 32.424394891599206 35.0 33.0 38.5 19.5 40.0 76-77 32.0846079207724 35.0 33.0 37.0 18.0 39.5 78-79 31.707815373844838 35.0 32.0 37.0 16.5 39.0 80-81 31.365348032948212 35.0 32.0 36.5 12.0 39.0 82-83 31.026190700971988 35.0 32.0 36.0 8.5 38.0 84-85 30.710096372239377 35.0 32.0 36.0 7.0 37.0 86-87 30.417895662686963 35.0 31.0 35.0 2.0 37.0 88-89 30.126516775506374 35.0 31.0 35.0 2.0 36.5 90-91 29.861146670383718 34.0 31.0 35.0 2.0 36.0 92-93 29.608415206106777 34.0 31.0 35.0 2.0 36.0 94-95 29.344183099409257 34.0 31.0 35.0 2.0 36.0 96-97 29.08195252573409 34.0 30.0 35.0 2.0 35.0 98-99 28.755405427139728 34.0 30.0 35.0 2.0 35.0 100-101 28.204459664539563 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39706667476425395 1101 2 0.42561834865025716 1101 3 0.4511811261088141 1101 4 0.43915885086372697 1101 5 0.41516433499948846 1101 6 0.4024563672146755 1101 7 0.4262881338252882 1101 8 0.45421525231854787 1101 9 0.4764259023995834 1101 10-11 0.4512934552258727 1101 12-13 0.45117395271899596 1101 14-15 0.5756022997477928 1101 16-17 0.5346791354090215 1101 18-19 0.49912863600272317 1101 20-21 0.47894586959738916 1101 22-23 0.5316007882286655 1101 24-25 0.4903290079059843 1101 26-27 0.47489951572264744 1101 28-29 0.4735593161169689 1101 30-31 0.4307500771524886 1101 32-33 0.3792632299839056 1101 34-35 0.4268789455390234 1101 36-37 0.41748840254444985 1101 38-39 0.32786630869829736 1101 40-41 0.34948064210691854 1101 42-43 0.26118341687357116 1101 44-45 0.15347020834116876 1101 46-47 0.19801749880027586 1101 48-49 0.11823414912779384 1101 50-51 0.04240879168123257 1101 52-53 0.1576622967192165 1101 54-55 0.35953869662725424 1101 56-57 0.404575725080349 1101 58-59 0.4048607399218369 1101 60-61 0.3908749967817684 1101 62-63 0.32394702016983246 1101 64-65 0.3285928303799537 1101 66-67 0.31104444836862655 1101 68-69 0.2920455856878448 1101 70-71 0.22191273894330266 1101 72-73 0.2855217843463933 1101 74-75 0.22480324854710432 1101 76-77 0.18944271329597484 1101 78-79 0.1511233052650951 1101 80-81 0.05817795548104954 1101 82-83 0.012856183049649417 1101 84-85 0.018486411188693097 1101 86-87 -0.04831823162848892 1101 88-89 -0.08109208845866078 1101 90-91 -0.18069004251119125 1101 92-93 -0.24160436835991206 1101 94-95 -0.39773625416042435 1101 96-97 -0.39643890335232257 1101 98-99 -0.5470418089820726 1101 100-101 -0.6903572951393713 1102 1 0.37791359310265804 1102 2 0.3886579111963684 1102 3 0.36740831769375504 1102 4 0.37926164118873373 1102 5 0.3652312747495188 1102 6 0.402533326104205 1102 7 0.3669992950106078 1102 8 0.3861263875767733 1102 9 0.5030279668886237 1102 10-11 0.4714086299344089 1102 12-13 0.4485730008239486 1102 14-15 0.5506256309185247 1102 16-17 0.5026537438552765 1102 18-19 0.47893522413082223 1102 20-21 0.4627838369277413 1102 22-23 0.4463844587051753 1102 24-25 0.5114418388921536 1102 26-27 0.4902782387097844 1102 28-29 0.47426463651431305 1102 30-31 0.37405049135404056 1102 32-33 0.4075577193133171 1102 34-35 0.4143722268248027 1102 36-37 0.3893807696321119 1102 38-39 0.3558325441106902 1102 40-41 0.3347247398208566 1102 42-43 0.3425152666186335 1102 44-45 0.31609479167978094 1102 46-47 0.25303081297006 1102 48-49 0.1426763342407753 1102 50-51 0.10430401626986452 1102 52-53 0.29879682705992394 1102 54-55 0.41887853516581686 1102 56-57 0.423524491923267 1102 58-59 0.4088110240709355 1102 60-61 0.4530766814475342 1102 62-63 0.43061529263358267 1102 64-65 0.36577084170586716 1102 66-67 0.3789137124695898 1102 68-69 0.32967293699966405 1102 70-71 0.2941848301543928 1102 72-73 0.27494462728067504 1102 74-75 0.23483732392536893 1102 76-77 0.19804674047485804 1102 78-79 0.13694866276912876 1102 80-81 0.07469045389827755 1102 82-83 0.025929862384263203 1102 84-85 0.05624382564840502 1102 86-87 0.0017924503606430164 1102 88-89 -0.053008256713557245 1102 90-91 -0.08358577601805806 1102 92-93 -0.15153635777607732 1102 94-95 -0.27211840317417924 1102 96-97 -0.2786700863011511 1102 98-99 -0.3595273773676553 1102 100-101 -0.42067239323701955 1103 1 0.36784436861779923 1103 2 0.3220392809862709 1103 3 0.35517654076531713 1103 4 0.3673157765423696 1103 5 0.3881196398384219 1103 6 0.3846578488982715 1103 7 0.41302057045829343 1103 8 0.3851728830115846 1103 9 0.4793561616180284 1103 10-11 0.4969187710422531 1103 12-13 0.4836489065740679 1103 14-15 0.5176108220439772 1103 16-17 0.5244636190775935 1103 18-19 0.516455573965132 1103 20-21 0.5110874504592289 1103 22-23 0.5111160678535853 1103 24-25 0.5664112660818148 1103 26-27 0.603822406437267 1103 28-29 0.5424181431501509 1103 30-31 0.5414158131749289 1103 32-33 0.5045788651977929 1103 34-35 0.5264246076128742 1103 36-37 0.5198700068124325 1103 38-39 0.49874789382921847 1103 40-41 0.42891797466833737 1103 42-43 0.40631902409185017 1103 44-45 0.37044131419704485 1103 46-47 0.3539551681149007 1103 48-49 0.31914120591009976 1103 50-51 0.24363971013577412 1103 52-53 0.35851763003204695 1103 54-55 0.4892260639395616 1103 56-57 0.5474243721109104 1103 58-59 0.5464357853125321 1103 60-61 0.4880264090599056 1103 62-63 0.4958181688595644 1103 64-65 0.42066306613160265 1103 66-67 0.4242904556018843 1103 68-69 0.3596023295615183 1103 70-71 0.3710551105177302 1103 72-73 0.4244832998920174 1103 74-75 0.38500285737293183 1103 76-77 0.293259454330844 1103 78-79 0.32015068339522657 1103 80-81 0.29778469425246357 1103 82-83 0.3172997806872253 1103 84-85 0.2962735328924673 1103 86-87 0.21557540550466925 1103 88-89 0.13840975822509805 1103 90-91 0.14734774803153883 1103 92-93 0.11484759500654107 1103 94-95 0.04976229797336984 1103 96-97 0.013084376652326313 1103 98-99 -0.0844491765819555 1103 100-101 -0.09505264290292104 1104 1 0.09377058658755288 1104 2 0.11080186375351175 1104 3 0.2159590018909654 1104 4 0.1268021841499376 1104 5 0.07278662024786797 1104 6 0.058270170954649814 1104 7 0.03545637456385009 1104 8 0.07872751480775264 1104 9 0.06652902931334381 1104 10-11 0.09152685608560773 1104 12-13 0.023224224344673416 1104 14-15 0.061126896895459026 1104 16-17 0.11485185128400843 1104 18-19 0.0678725090175405 1104 20-21 0.05850823034171526 1104 22-23 0.050330912109039616 1104 24-25 0.07395868240273273 1104 26-27 0.033614146940834644 1104 28-29 0.008609352322487496 1104 30-31 0.011627872969981468 1104 32-33 0.024887874892769446 1104 34-35 0.006231591372376499 1104 36-37 -0.026104368587930082 1104 38-39 -0.01750715525879798 1104 40-41 -0.06621552436750022 1104 42-43 -0.07281869455072609 1104 44-45 -0.08602302999451439 1104 46-47 -0.033170631452399846 1104 48-49 -0.08181050108711929 1104 50-51 -0.16628252276917266 1104 52-53 -0.0937705627430816 1104 54-55 0.04078960559019862 1104 56-57 -0.017692594708648812 1104 58-59 0.04020692226423961 1104 60-61 -0.004614397462894715 1104 62-63 0.005908413624119646 1104 64-65 -0.007075801653606106 1104 66-67 0.051034640753471194 1104 68-69 0.016351286273724952 1104 70-71 -0.04938050107158176 1104 72-73 -0.05117647208523124 1104 74-75 -0.029621185702879416 1104 76-77 -0.028731211441289872 1104 78-79 -0.04976207458174997 1104 80-81 -0.10709876464375867 1104 82-83 -0.11401137898430136 1104 84-85 -0.10320145627871824 1104 86-87 -0.06003307877363184 1104 88-89 -0.1320106450103431 1104 90-91 -0.19549788532422596 1104 92-93 -0.14722954413760903 1104 94-95 -0.1749312638536189 1104 96-97 -0.22362750314200142 1104 98-99 -0.18618403639228376 1104 100-101 -0.24914008175571922 1105 1 0.2501961103494281 1105 2 0.2601901741684216 1105 3 0.24422091818103198 1105 4 0.2719090905524979 1105 5 0.25284109720477943 1105 6 0.2843211780578372 1105 7 0.2949463759987907 1105 8 0.32246057572127285 1105 9 0.39303309354828997 1105 10-11 0.3503495209165095 1105 12-13 0.31078791432398845 1105 14-15 0.4108190278104331 1105 16-17 0.41346230652953864 1105 18-19 0.41408513333262675 1105 20-21 0.41059180875312506 1105 22-23 0.35066952964242404 1105 24-25 0.4303661893453352 1105 26-27 0.443011858928017 1105 28-29 0.4344916518826025 1105 30-31 0.4438851102744863 1105 32-33 0.38877935124838103 1105 34-35 0.4442751855720388 1105 36-37 0.41184647157226095 1105 38-39 0.36701202260090326 1105 40-41 0.4265960937824502 1105 42-43 0.42640637121926517 1105 44-45 0.37883731704265955 1105 46-47 0.32680060312111436 1105 48-49 0.28105163545617273 1105 50-51 0.2111318673213347 1105 52-53 0.3242388291179381 1105 54-55 0.44460773750629556 1105 56-57 0.4778257187942714 1105 58-59 0.5189149454026065 1105 60-61 0.5139673579913548 1105 62-63 0.47882867679602015 1105 64-65 0.49074391848731835 1105 66-67 0.47624221002096334 1105 68-69 0.45873822284185195 1105 70-71 0.41906393434477707 1105 72-73 0.45556871218163053 1105 74-75 0.412539934212127 1105 76-77 0.4228384571870336 1105 78-79 0.4245541386137788 1105 80-81 0.4072642666850257 1105 82-83 0.43746702861078646 1105 84-85 0.44597839838272435 1105 86-87 0.38338218151509906 1105 88-89 0.36841907746729774 1105 90-91 0.35695291122400263 1105 92-93 0.37809504127740823 1105 94-95 0.34046450362805203 1105 96-97 0.3222498589042324 1105 98-99 0.3153738786492788 1105 100-101 0.35619314493554555 1106 1 0.2531768103316523 1106 2 0.2917384970381569 1106 3 0.29664126463422136 1106 4 0.2322219695619765 1106 5 0.290265377897164 1106 6 0.2932002276840535 1106 7 0.33463594073452185 1106 8 0.3103912781377218 1106 9 0.3944628835520092 1106 10-11 0.37932629748063107 1106 12-13 0.38822781338587475 1106 14-15 0.4613511194473148 1106 16-17 0.46765007158668936 1106 18-19 0.4861223119724585 1106 20-21 0.4601146738542212 1106 22-23 0.49287205009243706 1106 24-25 0.4972771592392178 1106 26-27 0.509141192470203 1106 28-29 0.5126855628107094 1106 30-31 0.5710410340727492 1106 32-33 0.5655138655854728 1106 34-35 0.5388802686171275 1106 36-37 0.5630357803182591 1106 38-39 0.5529981412387173 1106 40-41 0.5955893844736337 1106 42-43 0.6279632147974894 1106 44-45 0.6797375364482079 1106 46-47 0.67413930635513 1106 48-49 0.5529749173577088 1106 50-51 0.5479951517839297 1106 52-53 0.6148029359346836 1106 54-55 0.6125706380658329 1106 56-57 0.6696931274474878 1106 58-59 0.7038914732722432 1106 60-61 0.7167207197874816 1106 62-63 0.6938971845670565 1106 64-65 0.6969010023022122 1106 66-67 0.6825468566584831 1106 68-69 0.7007014277895465 1106 70-71 0.7150335145027853 1106 72-73 0.7003168887908799 1106 74-75 0.7309877667848284 1106 76-77 0.7553229462343225 1106 78-79 0.6924787100673768 1106 80-81 0.6953647183353056 1106 82-83 0.6910520441242305 1106 84-85 0.7060982575205053 1106 86-87 0.664767151452903 1106 88-89 0.7708326909153804 1106 90-91 0.6815292988980524 1106 92-93 0.6528009294476043 1106 94-95 0.7041699214514132 1106 96-97 0.7615533436616033 1106 98-99 0.745494294088509 1106 100-101 0.7387396688996333 1107 1 0.2206617470108796 1107 2 0.21709645499492325 1107 3 0.1998329131260661 1107 4 0.23725711875652422 1107 5 0.21507973972844496 1107 6 0.23839118626387545 1107 7 0.26112190505479305 1107 8 0.2957382073830672 1107 9 0.3701514731800515 1107 10-11 0.3294356158925069 1107 12-13 0.3305480752674512 1107 14-15 0.44394446595894976 1107 16-17 0.3551222683505628 1107 18-19 0.3599990608246131 1107 20-21 0.42687674834050426 1107 22-23 0.4639173076393419 1107 24-25 0.4113995934515202 1107 26-27 0.46158627975824373 1107 28-29 0.45601606788719806 1107 30-31 0.46935535120489646 1107 32-33 0.46491184925969975 1107 34-35 0.4718843234681813 1107 36-37 0.4829853498194083 1107 38-39 0.5201588025261543 1107 40-41 0.4793651724976371 1107 42-43 0.5001996086999796 1107 44-45 0.5739247918495991 1107 46-47 0.6357729197900781 1107 48-49 0.4785885167702517 1107 50-51 0.5016611530708559 1107 52-53 0.5734207821013655 1107 54-55 0.6409119663745173 1107 56-57 0.5538399335563824 1107 58-59 0.585499297381709 1107 60-61 0.679630637802596 1107 62-63 0.6567808771875434 1107 64-65 0.6424741338444093 1107 66-67 0.650739607470264 1107 68-69 0.6650160335521065 1107 70-71 0.6490694526844294 1107 72-73 0.6033037930177869 1107 74-75 0.5746006327601023 1107 76-77 0.5622384047774673 1107 78-79 0.5844166958897006 1107 80-81 0.6285900925703203 1107 82-83 0.5862630861790343 1107 84-85 0.6078025161991754 1107 86-87 0.5747753446940855 1107 88-89 0.6212217121640968 1107 90-91 0.6452966113383489 1107 92-93 0.6469776608018734 1107 94-95 0.659942183684862 1107 96-97 0.6847368354555101 1107 98-99 0.7560823082257215 1107 100-101 0.7877632261676055 1108 1 0.21765127557519293 1108 2 0.24672479479055553 1108 3 0.25845042463470236 1108 4 0.2628695451958123 1108 5 0.2681858625914302 1108 6 0.2692705600567109 1108 7 0.2897771561919953 1108 8 0.27864196047502077 1108 9 0.2858503294787198 1108 10-11 0.34524670688582404 1108 12-13 0.335288469223606 1108 14-15 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70-71 -1.5510308804389794 2316 72-73 -1.4841437123351895 2316 74-75 -1.3374107370389083 2316 76-77 -1.23952547665046 2316 78-79 -1.2310632719349535 2316 80-81 -1.1767920895645076 2316 82-83 -1.111766032934991 2316 84-85 -1.19323145416433 2316 86-87 -1.1319446686435946 2316 88-89 -1.067614969982973 2316 90-91 -1.0567129006839444 2316 92-93 -1.13943568429179 2316 94-95 -1.4078398611798768 2316 96-97 -1.1394414585932715 2316 98-99 -0.9801029079644721 2316 100-101 -0.9588193344809426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127487.0 3 27170.0 4 21346.0 5 19274.0 6 20977.0 7 22350.0 8 24933.0 9 30281.0 10 37151.0 11 45603.0 12 57857.0 13 69387.0 14 77811.0 15 93169.0 16 115406.0 17 146324.0 18 116143.0 19 69672.0 20 62659.0 21 63135.0 22 66058.0 23 72029.0 24 82183.0 25 97872.0 26 117450.0 27 143145.0 28 175438.0 29 217301.0 30 273288.0 31 352584.0 32 467408.0 33 637648.0 34 915677.0 35 1406065.0 36 2158258.0 37 3146185.0 38 3040414.0 39 677751.0 40 387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.127248478694796 20.609394369415053 14.552212308830677 21.711144843059476 2 16.103018246099886 21.04779258524457 39.83028401310762 23.018905155547927 3 18.201057177083765 26.80184382897755 30.74546636892507 24.251632625013617 4 12.47606554384413 17.643423499609955 39.15130747759323 30.729203478952684 5 12.39773523886263 38.00254307689552 35.19136954393431 14.408352140307546 6 28.903635239025565 38.4803238879103 18.343677137316288 14.272363735747847 7 25.82103041115381 33.55291594113375 23.100089904733412 17.525963742979034 8 25.265176006281408 34.470209948535604 21.184678070172627 19.07993597501035 9 25.48825871593406 16.44198896472629 22.424569356567762 35.64518296277189 10-11 23.454660893619273 27.090126564848166 29.04730300760574 20.407909533926823 12-13 24.601562779164325 25.28842818884552 29.03026202186921 21.07974701012095 14-15 22.050539547707697 26.735881025881092 27.533458365302355 23.680121061108856 16-17 20.367813628419228 29.896686024361024 28.097528047777647 21.6379722994421 18-19 20.26224640538792 28.084580361585644 31.32768253811959 20.325490694906847 20-21 21.685457815892416 27.275372497310258 30.996161265300938 20.043008421496392 22-23 21.21687654475439 27.237817421313654 31.2209239168899 20.324382117042052 24-25 20.775019668413353 27.53025699133838 31.158647310081918 20.536076030166353 26-27 20.432112071572284 27.554276756785175 31.193505128412834 20.82010604322971 28-29 20.6631740071968 27.42851219401527 31.348522398573248 20.55979140021468 30-31 20.945009509525544 27.935533981598137 30.81883258723821 20.30062392163811 32-33 20.453143040955705 27.681078073574085 31.205088440319944 20.660690445150266 34-35 20.84055940137673 27.404342528685056 31.100252608224956 20.65484546171326 36-37 21.24864445740359 27.62780266287686 30.489442859529714 20.634110020189834 38-39 20.95180899459212 27.856731494926628 30.655652436516593 20.535807073964655 40-41 20.79265084398686 27.627325399600224 30.756303564916678 20.823720191496236 42-43 20.76205483451977 27.766989447014012 30.512569682097556 20.958386036368655 44-45 20.988276688127943 27.958837213696796 30.169937904638385 20.882948193536873 46-47 21.29201037737228 28.161139401499952 29.637421339839193 20.909428881288576 48-49 21.2638686863727 28.101841865771192 29.441129477338723 21.193159970517385 50-51 21.27059082448166 28.206255987087538 29.11919632141834 21.403956867012464 52-53 21.20187289411064 28.38823371786129 29.061768482474115 21.348124905553952 54-55 21.228345579397466 28.890176688361613 28.975998141488716 20.905479590752208 56-57 21.1871804244251 28.475665270175732 28.936657332862183 21.400496972536985 58-59 21.143450478052966 28.05322562870102 29.25764180718886 21.545682086057152 60-61 21.390665397542257 28.36407403318699 29.01499010186917 21.23027046740158 62-63 21.080688882321713 28.398867074819613 29.1232644564339 21.397179586424777 64-65 21.15975322246115 28.265807269595395 29.140549316115578 21.43389019182788 66-67 21.04385930845211 28.300781972769233 28.99831159472311 21.657047124055552 68-69 21.525707355136106 28.622885793393984 28.696825223669393 21.15458162780052 70-71 21.53316932658398 28.27224619136092 28.7337726126861 21.460811869369 72-73 21.256914525799186 28.366831773293157 28.904907272217955 21.471346428689696 74-75 21.26776684332429 28.536223387880575 28.788115693932838 21.4078940748623 76-77 21.411029284095324 28.61059850034502 28.495726538757772 21.48264567680188 78-79 21.46125879374889 28.58195680793658 28.703966753507608 21.252817644806917 80-81 21.347236954043463 28.510722370120757 28.69586189875384 21.446178777081943 82-83 21.185644696052655 28.513196178726457 28.915856574601356 21.385302550619528 84-85 21.141447079779148 28.49869484304443 28.692670289276556 21.667187787899866 86-87 21.343576900901812 28.602282176694576 28.581875942913804 21.472264979489804 88-89 21.143704451863666 28.539965980612507 28.737398991867042 21.578930575656784 90-91 20.958974703652 28.33570973142866 28.82942801161384 21.875887553305503 92-93 21.109619932817868 28.585201388833497 28.615596668693016 21.689582009655616 94-95 21.057328999300363 28.26009153669068 28.827807184132347 21.854772279876606 96-97 21.038784273097104 28.400706269376414 28.693272199685467 21.86723725784102 98-99 20.927123328155478 28.35120736993641 28.690104923093067 22.031564378815045 100-101 20.97943184366424 28.385895738387752 28.576254144770274 22.058418273177736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14776.0 1 9598.5 2 6654.5 3 8526.5 4 6419.5 5 5343.0 6 8391.5 7 15147.5 8 23365.0 9 27065.0 10 23109.5 11 18553.5 12 18378.0 13 19640.0 14 20930.5 15 22042.0 16 23152.5 17 24727.0 18 26934.5 19 29520.5 20 32734.5 21 37842.0 22 46646.5 23 61263.5 24 77305.0 25 94814.0 26 121088.5 27 155510.5 28 201682.0 29 245427.0 30 274262.5 31 307060.5 32 347268.5 33 382903.0 34 416894.5 35 452570.0 36 479278.5 37 501498.5 38 524584.0 39 536719.0 40 547363.0 41 549674.5 42 540405.0 43 535172.0 44 526010.5 45 516478.0 46 509875.0 47 500874.5 48 489894.5 49 482143.5 50 474392.0 51 451007.0 52 413857.0 53 378240.0 54 353604.5 55 338094.0 56 309237.5 57 271401.0 58 241537.5 59 216411.5 60 190098.5 61 160870.0 62 134547.5 63 110293.0 64 89927.5 65 73881.0 66 59030.0 67 45421.0 68 34370.0 69 26230.0 70 19673.5 71 14572.5 72 11212.0 73 8622.0 74 6477.0 75 4903.5 76 3626.5 77 2778.5 78 2176.0 79 1636.0 80 1277.5 81 1043.5 82 806.5 83 651.5 84 540.0 85 446.0 86 397.0 87 340.5 88 278.0 89 226.0 90 192.5 91 168.5 92 138.0 93 122.5 94 104.0 95 78.5 96 64.5 97 56.5 98 46.0 99 26.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014108931411240961 2 0.004347747631360166 3 0.003805096423235514 4 0.0059037836257417 5 0.0027328699397121043 6 0.00716561113379059 7 0.0012879793735006808 8 0.004073153044116366 9 0.01040190448344966 10-11 0.007195031982423854 12-13 0.008528777120465169 14-15 0.0034062804750957095 16-17 0.006060694818452442 18-19 0.004017580330031312 20-21 8.630115599090857E-4 22-23 0.0010885713994307784 24-25 0.0070838865542537455 26-27 0.0012618275080488904 28-29 9.806949544421428E-4 30-31 5.622651072134952E-4 32-33 0.0014122007343966856 34-35 0.0016181466748295356 36-37 0.0013174002221339452 38-39 0.0017946717666291211 40-41 0.002997657577411483 42-43 0.0029943885942300096 44-45 0.007969780996433147 46-47 0.01434756718348855 48-49 0.005485353778513052 50-51 0.0059037836257417 52-53 0.014076241579426222 54-55 0.008594156784094645 56-57 0.005684761752582954 58-59 0.0072081079151497494 60-61 0.0014154697175781594 62-63 0.0023275160252093524 64-65 0.0023994336552017764 66-67 0.0024190475542906187 68-69 0.0021215700847765024 70-71 0.0024648133188312524 72-73 0.0013762419194004738 74-75 0.0016181466748295356 76-77 0.00844378355774685 78-79 0.006740643320198994 80-81 0.01271961355911459 82-83 0.01545575248200817 84-85 0.008695495262720333 86-87 0.0060803087175412855 88-89 0.0030597682578594855 90-91 0.0027459458724379995 92-93 9.022393580867714E-4 94-95 0.0013533590371301572 96-97 0.001712947187092276 98-99 0.006397400086144245 100-101 0.011483937916517492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5295276E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.931208192569926 #Duplication Level Percentage of deduplicated Percentage of total 1 69.70062440285501 32.71134515002508 2 16.527021514397124 15.512661749905075 3 6.102043841395983 8.591288697622318 4 2.6598052286379135 4.993114917475679 5 1.4645416063667882 3.4366353517540262 6 0.8426988303258873 2.3729324549795625 7 0.5455243330120025 1.7921481232689396 8 0.38564583797036966 1.4479060088308404 9 0.27321552286464773 1.154010112650237 >10 1.3094882736367037 11.035233853457694 >50 0.10412671061145667 3.3820292376995367 >100 0.07407347718445963 6.872307662495753 >500 0.0074782650376782715 2.414026310411901 >1k 0.0035865701646343 2.8558342130490453 >5k 5.5815795297362776E-5 0.21973698478929293 >10k+ 6.976974412170347E-5 1.2087891715849692 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67040 0.43830526497200833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56409 0.3688001445675122 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32389 0.21175819252951042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.537966362947619E-6 0.0 5 0.0 2.6151865451790474E-5 0.0 6.537966362947619E-6 0.0 6 0.0 2.6151865451790474E-5 0.0 6.537966362947619E-6 0.0 7 0.0 2.6151865451790474E-5 0.0 1.9613899088842856E-5 0.0 8 0.0 2.6151865451790474E-5 0.0 1.9613899088842856E-5 0.0 9 0.0 2.6151865451790474E-5 0.0 5.884169726652857E-5 0.0 10-11 0.0 3.268983181473809E-5 0.0 8.826254589979285E-5 0.0 12-13 6.537966362947619E-6 3.922779817768571E-5 0.0 1.1114542817010952E-4 0.0 14-15 1.3075932725895237E-5 5.230373090358095E-5 0.0 1.6671814225516427E-4 0.0 16-17 1.3075932725895237E-5 6.211068044800237E-5 0.0 1.9940797406990237E-4 0.0 18-19 1.3075932725895237E-5 6.537966362947619E-5 0.0 2.451737386105357E-4 0.0 20-21 1.3075932725895237E-5 6.537966362947619E-5 0.0 2.90939503151169E-4 0.0 22-23 1.3075932725895237E-5 6.537966362947619E-5 0.0 4.315057799545428E-4 0.0 24-25 1.3075932725895237E-5 6.537966362947619E-5 0.0 6.636035858391833E-4 0.0 26-27 1.3075932725895237E-5 6.537966362947619E-5 0.0 0.0010526125844345666 0.0 28-29 1.3075932725895237E-5 6.537966362947619E-5 0.0 0.0022131016138577687 0.0 30-31 1.3075932725895237E-5 6.537966362947619E-5 0.0 0.0052990217371690455 0.0 32-33 1.3075932725895237E-5 6.537966362947619E-5 0.0 0.01171276673922066 0.0 34-35 1.3075932725895237E-5 6.537966362947619E-5 0.0 0.022555983952169283 0.0 36-37 1.3075932725895237E-5 7.19176299924238E-5 0.0 0.039642959041732886 0.0 38-39 1.3075932725895237E-5 9.153152908126666E-5 0.0 0.06270236640384914 0.0 40-41 1.3075932725895237E-5 1.046074618071619E-4 0.0 0.08806967589208589 0.0 42-43 1.9613899088842856E-5 1.1768339453305713E-4 0.0 0.112894334172198 0.0 44-45 1.9613899088842856E-5 1.2095237771453095E-4 0.0 0.14357701031351117 0.0 46-47 1.9613899088842856E-5 1.2749034407747855E-4 0.0 0.18097090892639006 0.0 48-49 2.2882882270316665E-5 1.372972936219E-4 0.0 0.22130035443623247 0.0 50-51 2.6151865451790474E-5 1.405662768033738E-4 0.0 0.2630975733945566 0.0 52-53 2.6151865451790474E-5 1.5037322634779522E-4 0.0 0.3053034152505649 0.0 54-55 2.6151865451790474E-5 1.5364220952926905E-4 0.0 0.35349476531185187 0.0 56-57 2.6151865451790474E-5 1.6344915907369047E-4 0.0 0.41184284611797783 0.0 58-59 2.6151865451790474E-5 1.6344915907369047E-4 0.0 0.47738595890652774 0.0 60-61 2.6151865451790474E-5 1.6344915907369047E-4 0.0 0.5471950947469009 0.0 62-63 2.6151865451790474E-5 1.6344915907369047E-4 0.0 0.6178508972312758 0.0 64-65 2.6151865451790474E-5 1.765250917995857E-4 0.0 0.6948517960708914 0.0 66-67 2.6151865451790474E-5 1.8306305816253332E-4 0.0 0.786782141100298 0.0 68-69 2.6151865451790474E-5 1.8306305816253332E-4 0.0 0.8913209542606488 0.0 70-71 2.6151865451790474E-5 1.8633204134400715E-4 0.0 1.0087232162400992 0.0 72-73 2.6151865451790474E-5 1.8960102452548095E-4 0.0 1.1343567778705006 0.0 74-75 2.6151865451790474E-5 1.8960102452548095E-4 0.0 1.2630304938596728 0.0 76-77 2.6151865451790474E-5 2.092149236143238E-4 6.537966362947619E-6 1.4068069121472537 0.0 78-79 2.6151865451790474E-5 2.157528899772714E-4 6.537966362947619E-6 1.5733387223610742 0.0 80-81 2.6151865451790474E-5 2.3209780588464044E-4 6.537966362947619E-6 1.7600172759223174 0.0 82-83 3.268983181473809E-5 2.484427217920095E-4 6.537966362947619E-6 1.9604549796943842 0.0 84-85 3.268983181473809E-5 2.484427217920095E-4 6.537966362947619E-6 2.164723931755138 0.0 86-87 3.268983181473809E-5 2.517117049734833E-4 6.537966362947619E-6 2.3857987263518488 0.0 88-89 3.268983181473809E-5 2.6151865451790474E-4 6.537966362947619E-6 2.638219800675712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 32055 0.0 59.6913 1 GTATCAA 62050 0.0 43.59062 1 GTGGTAT 12155 0.0 34.50826 1 ATCAACG 77875 0.0 34.26773 3 TCAACGC 78500 0.0 34.008007 4 TATCAAC 80740 0.0 33.1494 2 CAACGCA 81695 0.0 32.706314 5 TGGTATC 12630 0.0 31.890623 2 AACGCAG 86435 0.0 31.11597 6 TATAACG 1485 0.0 28.14665 2 CGCAGAG 96840 0.0 27.420885 8 ACGCAGA 97665 0.0 27.3013 7 GTACATG 63640 0.0 25.199364 1 GCAGAGT 103015 0.0 25.039392 9 TACATGG 62170 0.0 24.676989 2 GTACCTG 11315 0.0 23.971703 1 TACCTGG 11310 0.0 23.097795 2 ACATGGG 64495 0.0 22.861115 3 GAGTACT 67600 0.0 21.646849 12-13 CAGAGTA 101070 0.0 21.417553 10-11 >>END_MODULE