FastQCFastQC Report
Wed 25 May 2016
SRR2049429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15295276
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656430.42917172596297054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576960.3772145072766258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371400.24282007071987455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA317450.059.5232471
GTATCAA604400.042.3189431
GTGGTAT126400.033.8384931
TGGTATC127550.032.5474132
ATCAACG795350.031.9085243
TCAACGC807000.031.435914
TATAACG15400.031.1520162
TATCAAC826050.030.8093012
CAACGCA835450.030.4791135
AACGCAG891200.028.8009746
CGCAGAG1011750.025.0683148
ACGCAGA1022850.025.0005867
GTACCTG113650.023.5426041
TACCTGG111650.023.4836642
GTACATG620050.023.0857431
GCAGAGT1087900.022.8292929
TACATGG609250.022.3832512
ATAACGC23200.021.2924273
ACATGGG625650.021.021873
AGAGTAC1025150.020.91862310-11
Produced by FastQC (version 0.11.5)