##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15295276 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21671691311749 33.0 31.0 34.0 31.0 34.0 2 32.39130519776172 34.0 31.0 34.0 31.0 34.0 3 32.45812916354043 34.0 31.0 34.0 31.0 34.0 4 36.07753263164391 37.0 35.0 37.0 35.0 37.0 5 35.99086201517383 37.0 35.0 37.0 35.0 37.0 6 36.036041847169024 37.0 35.0 37.0 35.0 37.0 7 35.97253550704152 37.0 35.0 37.0 35.0 37.0 8 35.96532288792958 37.0 35.0 37.0 35.0 37.0 9 37.60634054593065 39.0 37.0 39.0 35.0 39.0 10-11 37.601553512339365 39.0 37.0 39.0 35.0 39.0 12-13 37.59365130122529 39.0 37.0 39.0 35.0 39.0 14-15 38.85122367193635 40.0 38.0 41.0 35.0 41.0 16-17 38.83656983371859 40.0 38.0 41.0 35.0 41.0 18-19 38.877125885142576 40.0 38.0 41.0 35.0 41.0 20-21 38.86916604185501 40.0 38.0 41.0 35.0 41.0 22-23 38.78652944870037 40.0 38.0 41.0 35.0 41.0 24-25 38.69122240749366 40.0 38.0 41.0 34.5 41.0 26-27 38.53016012917975 40.0 38.0 41.0 34.0 41.0 28-29 38.35192274398971 40.0 38.0 41.0 34.0 41.0 30-31 38.157968414561466 40.0 38.0 41.0 34.0 41.0 32-33 37.898418832062916 40.0 37.0 41.0 33.5 41.0 34-35 37.716953620189656 40.0 37.0 41.0 33.0 41.0 36-37 37.523280063726865 40.0 37.0 41.0 33.0 41.0 38-39 37.65817122227804 40.0 37.0 41.0 33.0 41.0 40-41 37.56214677002232 40.0 37.0 41.0 33.0 41.0 42-43 37.36058773963936 40.0 37.0 41.0 33.0 41.0 44-45 37.10688339327777 40.0 37.0 41.0 32.0 41.0 46-47 36.748455765034905 40.0 36.0 41.0 31.0 41.0 48-49 36.452370293939126 40.0 36.0 41.0 30.5 41.0 50-51 36.21701481555482 39.0 36.0 41.0 30.0 41.0 52-53 36.039156599723995 39.0 35.0 41.0 30.0 41.0 54-55 35.934299354911936 39.0 35.0 40.5 29.5 41.0 56-57 35.83957608872177 39.0 35.0 41.0 29.0 41.0 58-59 35.60406994290263 39.0 35.0 40.0 28.5 41.0 60-61 35.36011942510877 38.0 35.0 40.0 28.0 41.0 62-63 35.082112411701495 38.0 35.0 40.0 28.0 41.0 64-65 34.82654873308596 37.5 34.0 40.0 28.0 41.0 66-67 34.4847816083868 37.0 34.0 40.0 26.5 41.0 68-69 34.161047142921774 36.5 34.0 39.5 26.0 41.0 70-71 33.82799414668948 36.0 34.0 39.0 26.0 41.0 72-73 33.45897184202495 35.5 33.5 39.0 26.0 40.0 74-75 33.089866766706265 35.0 33.0 38.0 25.5 40.0 76-77 31.096292443496935 33.5 30.0 35.5 22.5 38.5 78-79 32.39314239899954 35.0 32.5 37.0 25.0 39.0 80-81 32.39903680064354 35.0 33.0 36.5 25.0 39.0 82-83 32.132898190264754 35.0 33.0 36.0 25.0 38.5 84-85 31.879603970533125 35.0 33.0 36.0 24.5 37.0 86-87 31.62740191808242 35.0 33.0 35.5 24.0 37.0 88-89 31.376573328915413 35.0 33.0 35.0 23.5 36.5 90-91 31.12820752629766 35.0 32.0 35.0 22.0 36.0 92-93 30.90625455205908 35.0 32.0 35.0 20.0 36.0 94-95 30.670745987192383 35.0 32.0 35.0 18.5 36.0 96-97 30.460129879316987 34.0 32.0 35.0 16.0 35.0 98-99 30.203987623368157 34.0 32.0 35.0 4.5 35.0 100-101 29.62220864795117 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3912313688995184 1101 2 0.39309643190969723 1101 3 0.37221997148891006 1101 4 0.3056728570807081 1101 5 0.3020070959789152 1101 6 0.32982944020639593 1101 7 0.32319783935001567 1101 8 0.3420379093412578 1101 9 0.3925181311956436 1101 10-11 0.3750084570198027 1101 12-13 0.3224436849472099 1101 14-15 0.4252169448209031 1101 16-17 0.4245563891542119 1101 18-19 0.38226318001919424 1101 20-21 0.3636379605967548 1101 22-23 0.29784017657411255 1101 24-25 0.27247406228784143 1101 26-27 0.27128089733950134 1101 28-29 0.28824481959324544 1101 30-31 0.22994710741977542 1101 32-33 0.24611086343220734 1101 34-35 0.18538145708065912 1101 36-37 0.2036062743845477 1101 38-39 0.2895935892046282 1101 40-41 0.3336389223621481 1101 42-43 0.37759296325484115 1101 44-45 0.3593796082918672 1101 46-47 0.35581356671266207 1101 48-49 0.2789223822002498 1101 50-51 0.33732970071729085 1101 52-53 0.275775100680697 1101 54-55 0.213954884900609 1101 56-57 0.18312952008938765 1101 58-59 0.182413595872724 1101 60-61 0.16590043483682138 1101 62-63 0.20003081711454485 1101 64-65 0.15041563477168296 1101 66-67 0.08264195407563335 1101 68-69 0.037398067071883645 1101 70-71 0.053623242485834055 1101 72-73 0.025109958138664012 1101 74-75 -3.730566993311868E-4 1101 76-77 0.006828875374186083 1101 78-79 0.05631976391735094 1101 80-81 0.08728009618934607 1101 82-83 0.04380816404339072 1101 84-85 0.02315666783794157 1101 86-87 0.02683002058320838 1101 88-89 -0.0591046169075895 1101 90-91 0.001511390681823599 1101 92-93 -0.04097515226283477 1101 94-95 -0.026381815627573246 1101 96-97 -0.13433548870635903 1101 98-99 -0.17790284861446892 1101 100-101 -0.19429326680811698 1102 1 0.34234771800635144 1102 2 0.3162026517219019 1102 3 0.3165165401178385 1102 4 0.267254059849094 1102 5 0.2555095206067861 1102 6 0.25438687507183033 1102 7 0.24837685552553523 1102 8 0.25689422757082525 1102 9 0.3780858266047815 1102 10-11 0.3096606828406152 1102 12-13 0.2613079542927821 1102 14-15 0.30563285741131097 1102 16-17 0.3306164190710703 1102 18-19 0.31149203503208867 1102 20-21 0.31820712661377115 1102 22-23 0.29586343544217897 1102 24-25 0.29553453544978936 1102 26-27 0.2144492653675769 1102 28-29 0.23712538513191816 1102 30-31 0.17243302788960335 1102 32-33 0.18520790012515675 1102 34-35 0.1821157201639565 1102 36-37 0.11828648244591733 1102 38-39 0.2405947186932309 1102 40-41 0.25249148427467816 1102 42-43 0.26444773071952454 1102 44-45 0.20973986182595894 1102 46-47 0.1612668695418762 1102 48-49 -0.06116385472157759 1102 50-51 0.02623736818870981 1102 52-53 0.07783774084244754 1102 54-55 0.05639496831755508 1102 56-57 0.020947613187502157 1102 58-59 0.023880801110998107 1102 60-61 0.039469961356218164 1102 62-63 0.0475838986750432 1102 64-65 0.01101532833980201 1102 66-67 -0.022625991270551538 1102 68-69 -0.10192181503608566 1102 70-71 -0.09680754649025403 1102 72-73 -0.09130733463754126 1102 74-75 -0.10629982826973361 1102 76-77 -0.1324291489728182 1102 78-79 -0.0434783407495658 1102 80-81 0.013280230762902079 1102 82-83 0.03136095067744549 1102 84-85 -0.014994668819472423 1102 86-87 -0.06692858528604972 1102 88-89 -0.11985656892894525 1102 90-91 -0.13301100820443068 1102 92-93 -0.18126099626175218 1102 94-95 -0.17862016643618972 1102 96-97 -0.255784942171875 1102 98-99 -0.2822736401408754 1102 100-101 -0.2943676049932584 1103 1 0.3507019661976969 1103 2 0.37319877696570103 1103 3 0.29745299790009483 1103 4 0.2608597937757011 1103 5 0.25032318786969654 1103 6 0.29060952948256613 1103 7 0.26589803730563233 1103 8 0.2880680708414829 1103 9 0.3742556797226868 1103 10-11 0.3252320193090199 1103 12-13 0.2780436418177743 1103 14-15 0.3725409415681966 1103 16-17 0.3873041109139237 1103 18-19 0.3353839669688341 1103 20-21 0.28785306588655857 1103 22-23 0.27735692938109224 1103 24-25 0.2991104285715096 1103 26-27 0.23976561432005639 1103 28-29 0.2908801134385186 1103 30-31 0.2662080008859036 1103 32-33 0.2641758131587508 1103 34-35 0.1938512955899654 1103 36-37 0.18399752998781338 1103 38-39 0.2887358301473739 1103 40-41 0.3766116523225733 1103 42-43 0.39206139406956453 1103 44-45 0.37468322844271285 1103 46-47 0.3954901257316408 1103 48-49 0.37849508121718856 1103 50-51 0.43325592612489316 1103 52-53 0.3942195907328667 1103 54-55 0.3640050036028484 1103 56-57 0.4189536660905233 1103 58-59 0.34626432193026346 1103 60-61 0.37885117919994826 1103 62-63 0.40108432440248265 1103 64-65 0.34756748376009483 1103 66-67 0.318542777057111 1103 68-69 0.3104101242231039 1103 70-71 0.2606517234905752 1103 72-73 0.3254298045554833 1103 74-75 0.32102941223173787 1103 76-77 0.28514403034025904 1103 78-79 0.40050654749676795 1103 80-81 0.34594138161915566 1103 82-83 0.4102367546376371 1103 84-85 0.36509761636999016 1103 86-87 0.3425256063252533 1103 88-89 0.32036250730431703 1103 90-91 0.35882784492771336 1103 92-93 0.3400755854428503 1103 94-95 0.35729116229084923 1103 96-97 0.3130120789537436 1103 98-99 0.297827158860521 1103 100-101 0.3249825670505899 1104 1 0.3066888970365369 1104 2 0.30180684951590564 1104 3 0.29669548871272866 1104 4 0.21226058796248992 1104 5 0.22392011095320186 1104 6 0.2535268393429462 1104 7 0.2727618294394958 1104 8 0.25156635700211893 1104 9 0.3449332816915742 1104 10-11 0.3109473174390729 1104 12-13 0.25788744949987574 1104 14-15 0.3128069145033905 1104 16-17 0.3402305596579609 1104 18-19 0.2863598955561173 1104 20-21 0.29368371419906936 1104 22-23 0.273736996167365 1104 24-25 0.2650306007865595 1104 26-27 0.24616961864723663 1104 28-29 0.2553855570385224 1104 30-31 0.23580641514698186 1104 32-33 0.2497332286367353 1104 34-35 0.24659188722428382 1104 36-37 0.3096900217620444 1104 38-39 0.34229915309627756 1104 40-41 0.3722678725218529 1104 42-43 0.34933314174935504 1104 44-45 0.30914803860377305 1104 46-47 0.36013735452194595 1104 48-49 0.33353084783075104 1104 50-51 0.2931254944889261 1104 52-53 0.2791103377185351 1104 54-55 0.3307226791993543 1104 56-57 0.3292111244416773 1104 58-59 0.32181315677875233 1104 60-61 0.344963039855422 1104 62-63 0.3663329494420964 1104 64-65 0.41145405859690953 1104 66-67 0.3704667810363347 1104 68-69 0.30994817517277085 1104 70-71 0.28303933946960314 1104 72-73 0.25876591305095076 1104 74-75 0.29032623232460253 1104 76-77 0.18877179325199123 1104 78-79 0.2885769134554579 1104 80-81 0.2682619936754804 1104 82-83 0.32376505165755276 1104 84-85 0.2521746179194402 1104 86-87 0.18518359630118297 1104 88-89 0.1767025231582693 1104 90-91 0.18962907996704104 1104 92-93 0.11804985069585783 1104 94-95 0.10737575283035028 1104 96-97 0.10776583047671906 1104 98-99 0.13959896510867154 1104 100-101 0.1551345295954043 1105 1 0.27096731538230756 1105 2 0.283945509566891 1105 3 0.2695511565568012 1105 4 0.206271237563449 1105 5 0.20493530650954028 1105 6 0.21362835978928274 1105 7 0.22106523236477216 1105 8 0.2637141873456059 1105 9 0.2555546642726796 1105 10-11 0.23708361830671265 1105 12-13 0.2117592100876422 1105 14-15 0.27952448260835183 1105 16-17 0.2661735977212274 1105 18-19 0.2683012824833355 1105 20-21 0.266229629729402 1105 22-23 0.19006077836404955 1105 24-25 0.21926929370832227 1105 26-27 0.22311218893214146 1105 28-29 0.20072815088465745 1105 30-31 0.20866923238524748 1105 32-33 0.2772816583022504 1105 34-35 0.29146922478486914 1105 36-37 0.24284582571440438 1105 38-39 0.3055074452762696 1105 40-41 0.2838991637828272 1105 42-43 0.33961082607912374 1105 44-45 0.338355553616303 1105 46-47 0.3337833752471724 1105 48-49 0.3453955344918356 1105 50-51 0.3277207946963969 1105 52-53 0.3135111017641634 1105 54-55 0.31879610606769404 1105 56-57 0.2987596083535138 1105 58-59 0.28952871502405486 1105 60-61 0.2850364674847441 1105 62-63 0.3018525199585511 1105 64-65 0.3162721241989601 1105 66-67 0.31533617232739886 1105 68-69 0.24659408334532174 1105 70-71 0.25478830400837893 1105 72-73 0.30795207180476325 1105 74-75 0.27810992172392446 1105 76-77 0.3218187223294855 1105 78-79 0.34912421841121244 1105 80-81 0.34736947618907266 1105 82-83 0.3709751044520786 1105 84-85 0.354470357360011 1105 86-87 0.33702166490507324 1105 88-89 0.34843162677941564 1105 90-91 0.39461063861600465 1105 92-93 0.3683674906916856 1105 94-95 0.34945665511563817 1105 96-97 0.38832348815305906 1105 98-99 0.436201668543017 1105 100-101 0.5145932734551906 1106 1 0.2677781812984321 1106 2 0.24917251454389344 1106 3 0.21902142184227813 1106 4 0.1920747974225776 1106 5 0.20806897138893987 1106 6 0.2275211952937397 1106 7 0.2298546003536046 1106 8 0.23300615283018544 1106 9 0.3315139989124347 1106 10-11 0.282150252610343 1106 12-13 0.2571359247407301 1106 14-15 0.31394286140194794 1106 16-17 0.3152109474020435 1106 18-19 0.2738181474064163 1106 20-21 0.2301007012898637 1106 22-23 0.2500370579415474 1106 24-25 0.2754770365307877 1106 26-27 0.26076306240482694 1106 28-29 0.33932962430208136 1106 30-31 0.3290866325372406 1106 32-33 0.3437902869357501 1106 34-35 0.25671033741401317 1106 36-37 0.3516216641641208 1106 38-39 0.3988966104935443 1106 40-41 0.4285241620807838 1106 42-43 0.46760970432807625 1106 44-45 0.4597764415263441 1106 46-47 0.501938637095428 1106 48-49 0.49691729211470204 1106 50-51 0.4629410056805483 1106 52-53 0.4542383179853573 1106 54-55 0.43093688425738463 1106 56-57 0.39530130441729483 1106 58-59 0.4854999956390884 1106 60-61 0.5065291134104797 1106 62-63 0.47041556114144356 1106 64-65 0.46073065770746524 1106 66-67 0.4983097354724535 1106 68-69 0.46873294529147813 1106 70-71 0.4621950924403322 1106 72-73 0.4675117690590369 1106 74-75 0.43207163399647186 1106 76-77 0.41662689457769275 1106 78-79 0.4491297983666911 1106 80-81 0.4715051003210675 1106 82-83 0.5142456410864611 1106 84-85 0.476337101712641 1106 86-87 0.4594472285636897 1106 88-89 0.45865947129090046 1106 90-91 0.4778939538778353 1106 92-93 0.44425488519955536 1106 94-95 0.48336749937052303 1106 96-97 0.47845368196132654 1106 98-99 0.5158909673044008 1106 100-101 0.5504759977415006 1107 1 0.22695779313850295 1107 2 0.20982122619943055 1107 3 0.18965369985628655 1107 4 0.1830791849192579 1107 5 0.18597207776304714 1107 6 0.19023841810815156 1107 7 0.22041630825191305 1107 8 0.18314782324491574 1107 9 0.25858021693145616 1107 10-11 0.195409712917197 1107 12-13 0.18494245792627595 1107 14-15 0.19520577645439374 1107 16-17 0.2276148261554738 1107 18-19 0.2060311144329532 1107 20-21 0.16785708101112817 1107 22-23 0.19381394593774104 1107 24-25 0.21909170108714449 1107 26-27 0.21530175524303985 1107 28-29 0.23652855984421706 1107 30-31 0.2252306237136139 1107 32-33 0.26903966653245703 1107 34-35 0.18421212645098706 1107 36-37 0.23754844696986055 1107 38-39 0.2714300397566518 1107 40-41 0.28557844107361774 1107 42-43 0.28506110185926303 1107 44-45 0.2698716811925834 1107 46-47 0.26421738788878457 1107 48-49 0.164390596200235 1107 50-51 0.21461911348014695 1107 52-53 0.259770786079379 1107 54-55 0.295947585814865 1107 56-57 0.3398875743187446 1107 58-59 0.3614412705987107 1107 60-61 0.38610826666749176 1107 62-63 0.34076501278958915 1107 64-65 0.38979055284629993 1107 66-67 0.39548308665794707 1107 68-69 0.39338667402874705 1107 70-71 0.41018316309097 1107 72-73 0.41433367117102904 1107 74-75 0.4064386462472598 1107 76-77 0.3615307947160602 1107 78-79 0.4877947963475222 1107 80-81 0.5306636293610154 1107 82-83 0.5359224465990238 1107 84-85 0.5388504887395094 1107 86-87 0.5370391212052681 1107 88-89 0.43711307413565237 1107 90-91 0.5116888781105153 1107 92-93 0.5393075071704558 1107 94-95 0.6274624708215697 1107 96-97 0.6543509212863441 1107 98-99 0.6711968335756708 1107 100-101 0.7653066176433967 1108 1 0.14787943061023867 1108 2 0.14219052371202423 1108 3 0.11118009000448836 1108 4 0.1297870250059674 1108 5 0.10483490392422112 1108 6 0.12737862467334082 1108 7 0.15831250296840693 1108 8 0.14234515788601243 1108 9 0.22190459672409446 1108 10-11 0.14912387437104258 1108 12-13 0.1069055611198877 1108 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5 212.0 6 515.0 7 1461.0 8 3989.0 9 8368.0 10 14911.0 11 23934.0 12 36455.0 13 52004.0 14 64409.0 15 83469.0 16 107253.0 17 144902.0 18 120672.0 19 65423.0 20 49479.0 21 46933.0 22 47263.0 23 50295.0 24 58427.0 25 70497.0 26 88213.0 27 111719.0 28 144054.0 29 185919.0 30 240636.0 31 317033.0 32 424246.0 33 590623.0 34 867524.0 35 1356603.0 36 2178451.0 37 3418757.0 38 3525548.0 39 794500.0 40 460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.81218666107454 22.041563149726738 15.723705890533052 22.422544298665674 2 15.331697320441547 22.03447055712921 39.08269340358242 23.551138718846822 3 17.161178805724106 27.159790792470574 31.491438220366945 24.187592181438372 4 12.318781832620061 18.56883822272004 38.62905565771516 30.483324286944736 5 12.235380298000493 38.087721415684754 35.464679212133426 14.212219074181323 6 28.971370767275783 38.972014114943214 18.011112915622864 14.045502202158143 7 25.694855065054075 33.55103889593101 23.08941662772218 17.66468941129274 8 25.341239391984736 34.84061808896003 20.908922586153082 18.90921993290215 9 25.173020968070098 16.43467098556572 22.40571194100968 35.986596105354494 10-11 23.43431182095433 27.333033196992716 29.013160342173734 20.219494639879215 12-13 24.591955712338894 25.316205474160782 29.10093286319253 20.990905950307795 14-15 21.909519145589684 26.842236350201947 27.585784293294218 23.662460210914148 16-17 20.26646004949502 29.957677782342728 28.253883748158582 21.521978420003666 18-19 20.186643358367004 28.006555884248375 31.61924862115843 20.187552136226195 20-21 21.558400029394857 27.180336097088276 31.321257884053576 19.94000598946329 22-23 21.063153747601547 27.135839196363637 31.551781085872527 20.24922597016229 24-25 20.715460361252532 27.368379553470888 31.476966622083975 20.439193463192602 26-27 20.396524957639834 27.412788600238475 31.48338241475357 20.70730402736812 28-29 20.620492286240136 27.296775714055794 31.69408682631549 20.388645173388575 30-31 20.887629313464487 27.752369860127107 31.17575686489419 20.184243961514213 32-33 20.394345548718093 27.50068546996236 31.535116314780097 20.56985266653945 34-35 20.730921782338992 27.302356994996398 31.396554540757837 20.570166681906777 36-37 21.14839493973732 27.49561339746367 30.807626217167172 20.54836544563184 38-39 20.870587129194103 27.685441032419906 31.019773176116267 20.42419866226972 40-41 20.6501754221136 27.5357062777099 31.097937494395623 20.71618080578088 42-43 20.623202361232355 27.631581012017996 30.868615521974935 20.876601104774718 44-45 20.85639018236219 27.81450361506374 30.527426116199063 20.801680086375 46-47 21.101544120031505 28.074111432722876 29.9324872456585 20.891857201587115 48-49 21.080897907882164 27.98010478242074 29.758707001770258 21.180290307926835 50-51 21.160770778655387 28.085009842234932 29.3717102311352 21.382509147974485 52-53 21.073381794399772 28.28889663218112 29.311756550369196 21.325965023049914 54-55 21.19358541130889 28.737118044397775 29.203861227769483 20.865435316523847 56-57 21.184586800057183 28.300368993871 29.141405131877463 21.373639074194354 58-59 21.078671131959258 27.91688648031177 29.456240156603858 21.548202231125117 60-61 21.306769924158782 28.27702986768554 29.199257737610075 21.216942470545604 62-63 21.021578863611428 28.253923504044558 29.32052594875904 21.40397168358497 64-65 21.020040971968033 28.225015849038886 29.334064461137743 21.42087871785533 66-67 20.965053199244952 28.20502157980802 29.209837724812825 21.620087496134204 68-69 21.446711045522875 28.49001606505005 28.84780506431578 21.2154678251113 70-71 21.4454190756892 28.16353248965689 28.900715531955885 21.490332902698032 72-73 21.18251244134505 28.304119646651877 28.99881276253793 21.51455514946515 74-75 21.171039464861952 28.461396381567656 28.89060916020502 21.476954993365368 76-77 21.23611475110745 28.56765562075385 28.664030482975654 21.532199145163048 78-79 21.313157422701472 28.52655555625659 28.835910177025276 21.324376844016665 80-81 21.169285260149817 28.45323041697203 28.813789427274557 21.563694895603593 82-83 21.037443166578377 28.445888175395655 29.015342837701713 21.501325820324254 84-85 21.011829249862675 28.479054462878324 28.761424473638904 21.7476918136201 86-87 21.144867932495163 28.591582889758527 28.685989443648623 21.577559734097687 88-89 20.956136067234784 28.477479402995304 28.793301994184826 21.773082535585083 90-91 20.782327502367593 28.34100083915956 28.859818510088058 22.01685314838479 92-93 20.951932478405887 28.553600446674476 28.646449503457116 21.84801757146252 94-95 20.84289475083859 28.242326160525376 28.84290691152003 22.07187217711601 96-97 20.807727697087515 28.457187905774333 28.690349038210737 22.044735358927415 98-99 20.608705109537873 28.39803978891533 28.74017350357117 22.253081597975623 100-101 20.60589963336711 28.48809329810894 28.571596223604384 22.334410844919567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14034.0 1 9359.0 2 6548.0 3 8171.5 4 6203.0 5 4914.0 6 8135.0 7 15965.5 8 24318.5 9 27934.0 10 24233.5 11 19164.0 12 18630.0 13 20033.5 14 21537.0 15 22991.0 16 24277.5 17 25325.0 18 27339.0 19 30071.5 20 33306.0 21 38244.0 22 46921.5 23 61148.5 24 77209.5 25 94590.0 26 121051.0 27 155933.0 28 202962.0 29 248065.0 30 277136.5 31 310213.5 32 349990.5 33 385661.0 34 420458.5 35 457290.0 36 484171.0 37 506084.0 38 528943.0 39 540967.0 40 550784.0 41 551293.5 42 542448.5 43 537653.0 44 530029.5 45 520488.5 46 513481.5 47 506728.0 48 494839.5 49 486176.0 50 478741.5 51 453475.5 52 411194.0 53 374270.5 54 350938.0 55 333308.0 56 303892.0 57 266839.5 58 237904.0 59 213517.5 60 186528.0 61 156013.0 62 129595.5 63 106046.5 64 85356.0 65 68933.5 66 54772.0 67 41668.5 68 31040.0 69 23379.5 70 17205.0 71 12198.0 72 8709.5 73 6462.0 74 4909.0 75 3700.0 76 2520.5 77 1758.0 78 1267.5 79 855.0 80 588.5 81 401.5 82 269.0 83 187.0 84 120.0 85 81.0 86 61.5 87 41.5 88 30.0 89 23.0 90 15.5 91 8.0 92 3.5 93 4.0 94 4.0 95 1.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046537244571461155 2 0.0024059716215647235 3 2.1575288997727142E-4 4 2.484427217920095E-4 5 0.0026282624779049427 6 0.0018306305816253334 7 0.0 8 1.3075932725895237E-5 9 1.3075932725895237E-4 10-11 3.268983181473809E-5 12-13 0.0 14-15 6.243757876614976E-4 16-17 0.0 18-19 5.8841697266528574E-5 20-21 5.393822249431786E-4 22-23 0.0 24-25 6.734105353836047E-4 26-27 0.00739770893967523 28-29 0.007489240468756497 30-31 0.0011441441135158332 32-33 0.006698146538839835 34-35 0.007874980484170407 36-37 0.0045406176390671215 38-39 0.0027067180742603144 40-41 0.002036576522058183 42-43 0.0013860488689448952 44-45 7.191762999242381E-4 46-47 6.014929053911809E-4 48-49 0.0022490604288539807 50-51 0.0014612354821187928 52-53 0.0020758043202358687 54-55 9.218532571756143E-4 56-57 2.3209780588464044E-4 58-59 8.041698626425572E-4 60-61 0.006237219910252029 62-63 0.0026282624779049427 64-65 0.0012062547939638356 66-67 0.00579263819757159 68-69 0.007884787433714829 70-71 0.0028211324856118975 72-73 6.864864681094999E-4 74-75 0.0034716601387251855 76-77 0.0020431144884211308 78-79 0.002415778571109145 80-81 8.989703749052975E-4 82-83 1.8633204134400715E-4 84-85 6.309137540244452E-4 86-87 0.005145379527639776 88-89 0.0029747746951411663 90-91 0.0013795109025819476 92-93 1.372972936219E-4 94-95 5.263062922172833E-4 96-97 3.8900899859538334E-4 98-99 5.655340903949691E-4 100-101 2.2882882270316665E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5295276E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.411376816843415 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64826168739549 30.720007024907485 2 17.684266932981835 16.061338188465637 3 6.437606057677783 8.770216634507989 4 2.9172354707485777 5.29902716902501 5 1.5287364907418617 3.471101441736877 6 0.9144229989755981 2.4915124425881343 7 0.5676478723235192 1.8044370000553926 8 0.41144933274043555 1.4947584552091768 9 0.28983863772020074 1.1845774434233343 >10 1.4036716021171312 11.491243386316846 >50 0.10738526508532649 3.3880123970848794 >100 0.07768447270403701 7.0387577561897245 >500 0.007862539655023466 2.433825488951815 >1k 0.003800594397841516 2.894623821022531 >5k 4.3348245087158785E-5 0.1484514100101661 >10k+ 8.669649017431757E-5 1.3081099405050687 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65643 0.42917172596297054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57696 0.3772145072766258 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37140 0.24282007071987455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3075932725895237E-5 0.0 4 0.0 0.0 0.0 3.268983181473809E-5 0.0 5 0.0 0.0 0.0 3.922779817768571E-5 0.0 6 0.0 0.0 0.0 3.922779817768571E-5 0.0 7 0.0 1.3075932725895237E-5 0.0 3.922779817768571E-5 0.0 8 0.0 1.3075932725895237E-5 0.0 3.922779817768571E-5 0.0 9 1.3075932725895237E-5 1.9613899088842856E-5 0.0 7.845559635537142E-5 0.0 10-11 1.3075932725895237E-5 1.9613899088842856E-5 0.0 8.826254589979285E-5 0.0 12-13 1.3075932725895237E-5 2.2882882270316665E-5 0.0 1.2095237771453093E-4 0.0 14-15 1.3075932725895237E-5 2.9420848633264284E-5 0.0 1.765250917995857E-4 0.0 16-17 1.3075932725895237E-5 3.268983181473809E-5 0.0 1.9940797406990237E-4 0.0 18-19 1.6344915907369047E-5 3.268983181473809E-5 0.0 2.2882882270316664E-4 0.0 20-21 2.6151865451790474E-5 3.922779817768571E-5 0.0 2.5824967133643095E-4 0.0 22-23 2.6151865451790474E-5 3.922779817768571E-5 0.0 3.9881594813980474E-4 0.0 24-25 2.6151865451790474E-5 3.922779817768571E-5 0.0 6.407207035688666E-4 0.0 26-27 3.268983181473809E-5 3.922779817768571E-5 0.0 0.0010231917358013024 0.0 28-29 3.922779817768571E-5 4.576576454063333E-5 0.0 0.0021804117820430307 0.0 30-31 3.922779817768571E-5 5.230373090358095E-5 0.0 0.005207490208087778 0.0 32-33 3.922779817768571E-5 6.537966362947619E-5 0.0 0.011555855546509916 0.0 34-35 4.576576454063333E-5 6.864864681095E-5 0.0 0.02226177546583664 0.0 36-37 4.576576454063333E-5 8.499356271831904E-5 0.0 0.03929317784131519 0.0 38-39 4.576576454063333E-5 1.2749034407747855E-4 0.0 0.06220874994344659 0.0 40-41 4.576576454063333E-5 1.340283104404262E-4 0.0 0.08741261027260966 0.0 42-43 4.576576454063333E-5 1.438352599848476E-4 0.0 0.11211631617500724 0.0 44-45 4.576576454063333E-5 1.5037322634779522E-4 0.0 0.14264208112360965 0.0 46-47 4.576576454063333E-5 1.5037322634779522E-4 0.0 0.17998694498876647 6.537966362947619E-6 48-49 4.9034747722107136E-5 1.5037322634779522E-4 0.0 0.22021505201998315 6.537966362947619E-6 50-51 5.230373090358095E-5 1.5691119271074285E-4 0.0 0.2618847806342298 6.537966362947619E-6 52-53 5.884169726652857E-5 1.6018017589221665E-4 0.0 0.3039435182470719 6.537966362947619E-6 54-55 5.884169726652857E-5 1.6344915907369047E-4 0.0 0.35213159932517724 6.537966362947619E-6 56-57 6.537966362947619E-5 1.9287000770695475E-4 0.0 0.41032276893859254 6.537966362947619E-6 58-59 7.19176299924238E-5 2.092149236143238E-4 0.0 0.47563378392125777 6.537966362947619E-6 60-61 7.19176299924238E-5 2.092149236143238E-4 0.0 0.5452304358548352 6.537966362947619E-6 62-63 7.518661317389761E-5 2.124839067957976E-4 0.0 0.6155299191724295 6.537966362947619E-6 64-65 7.845559635537142E-5 2.1575288997727142E-4 0.0 0.6920960432489089 6.537966362947619E-6 66-67 7.845559635537142E-5 2.1902187315874522E-4 0.0 0.7836962209769867 6.537966362947619E-6 68-69 7.845559635537142E-5 2.2229085634021902E-4 0.0 0.8881238887091674 6.537966362947619E-6 70-71 7.845559635537142E-5 2.3536678906611427E-4 0.0 1.0051436796563853 6.537966362947619E-6 72-73 7.845559635537142E-5 2.517117049734833E-4 0.0 1.1303816943218283 6.537966362947619E-6 74-75 7.845559635537142E-5 2.6151865451790474E-4 0.0 1.2585519869010535 6.537966362947619E-6 76-77 7.845559635537142E-5 2.6805662088085234E-4 0.0 1.4020930383995687 6.537966362947619E-6 78-79 7.845559635537142E-5 2.778635704252738E-4 0.0 1.568647731495659 6.537966362947619E-6 80-81 7.845559635537142E-5 2.811325536067476E-4 0.0 1.755081111318292 6.537966362947619E-6 82-83 7.845559635537142E-5 2.811325536067476E-4 0.0 1.9551657649067593 6.537966362947619E-6 84-85 7.845559635537142E-5 2.811325536067476E-4 0.0 2.1596995046052125 6.537966362947619E-6 86-87 7.845559635537142E-5 2.811325536067476E-4 0.0 2.3809083275123637 6.537966362947619E-6 88-89 7.845559635537142E-5 2.844015367882214E-4 0.0 2.633545154726204 6.537966362947619E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 31745 0.0 59.523247 1 GTATCAA 60440 0.0 42.318943 1 GTGGTAT 12640 0.0 33.838493 1 TGGTATC 12755 0.0 32.547413 2 ATCAACG 79535 0.0 31.908524 3 TCAACGC 80700 0.0 31.43591 4 TATAACG 1540 0.0 31.152016 2 TATCAAC 82605 0.0 30.809301 2 CAACGCA 83545 0.0 30.479113 5 AACGCAG 89120 0.0 28.800974 6 CGCAGAG 101175 0.0 25.068314 8 ACGCAGA 102285 0.0 25.000586 7 GTACCTG 11365 0.0 23.542604 1 TACCTGG 11165 0.0 23.483664 2 GTACATG 62005 0.0 23.085743 1 GCAGAGT 108790 0.0 22.829292 9 TACATGG 60925 0.0 22.383251 2 ATAACGC 2320 0.0 21.292427 3 ACATGGG 62565 0.0 21.02187 3 AGAGTAC 102515 0.0 20.918623 10-11 >>END_MODULE