FastQCFastQC Report
Wed 25 May 2016
SRR2049428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12621562
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552560.43779050485193516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461750.3658421992460204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265870.21064746185931663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA270950.058.7851071
GTATCAA506300.042.3053441
TATAACG14000.035.9634362
TACCTGG114950.034.0487822
GTGGTAT104000.033.8917121
ATCAACG626900.033.7793243
TCAACGC634150.033.4767274
GTACCTG120850.033.096981
TATCAAC648200.032.821272
CAACGCA662000.032.1105425
TGGTATC107800.031.5043582
AACGCAG701200.030.5797526
ATAACGC18550.027.3999023
CGCAGAG776750.027.240238
ACGCAGA787550.027.0899687
CCCGAGG45700.026.713213
CCGAGGG47650.025.321854
GTACATG498950.024.6679651
TACATGG492050.024.026972
GCAGAGT864750.023.8089479
Produced by FastQC (version 0.11.5)