##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049428_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12621562 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.606597424312458 31.0 31.0 34.0 30.0 34.0 2 31.64131222427145 33.0 31.0 34.0 30.0 34.0 3 31.66529705277366 33.0 31.0 34.0 30.0 34.0 4 35.30343700724205 37.0 35.0 37.0 33.0 37.0 5 35.3305105976582 37.0 35.0 37.0 33.0 37.0 6 35.430102470676765 37.0 35.0 37.0 33.0 37.0 7 35.379968184603456 37.0 35.0 37.0 33.0 37.0 8 35.355005347198706 37.0 35.0 37.0 33.0 37.0 9 36.95431357862046 39.0 37.0 39.0 33.0 39.0 10-11 36.886501528099295 39.0 37.0 39.0 33.0 39.0 12-13 36.85626881997648 39.0 37.0 39.0 33.0 39.0 14-15 38.04004021055397 40.0 38.0 41.0 33.0 41.0 16-17 38.027675061137444 40.0 38.0 41.0 33.0 41.0 18-19 38.05686415833476 40.0 38.0 41.0 33.5 41.0 20-21 38.0178232298031 40.0 38.0 41.0 33.0 41.0 22-23 37.90039921366309 40.0 38.0 41.0 33.0 41.0 24-25 37.78841671894493 40.0 38.0 41.0 33.0 41.0 26-27 37.64844418622671 40.0 37.5 41.0 32.5 41.0 28-29 37.485443441944824 40.0 37.0 41.0 32.0 41.0 30-31 37.282927778669546 40.0 37.0 41.0 32.0 41.0 32-33 37.0083176709824 40.0 36.0 41.0 31.5 41.0 34-35 36.79927595332495 39.5 36.0 41.0 31.0 41.0 36-37 36.59986961201791 39.0 36.0 41.0 30.5 41.0 38-39 36.38385835287265 39.0 35.5 41.0 30.0 41.0 40-41 36.15323103432047 39.0 35.0 40.0 30.0 41.0 42-43 35.9142451623658 39.0 35.0 40.0 29.5 41.0 44-45 35.61656191999057 39.0 35.0 40.0 28.5 41.0 46-47 35.23605909474596 38.5 35.0 40.0 26.5 41.0 48-49 34.91569189296855 38.0 34.5 40.0 25.5 41.0 50-51 33.560479004104245 37.0 32.5 39.0 23.5 40.0 52-53 33.86542469941517 37.5 33.5 39.0 24.0 40.0 54-55 34.90993646428231 38.5 35.0 40.0 26.0 41.0 56-57 35.02000204095182 39.0 35.0 40.0 26.0 41.0 58-59 34.863042070387166 39.0 35.0 40.0 26.0 41.0 60-61 34.605801207489215 38.0 34.0 40.0 25.5 41.0 62-63 34.319912384853794 38.0 34.0 40.0 24.5 41.0 64-65 34.03235288944427 37.5 34.0 40.0 23.5 41.0 66-67 33.71057615531263 37.0 34.0 40.0 23.0 41.0 68-69 33.35237247180658 36.0 33.0 39.5 22.0 41.0 70-71 33.008961648328466 36.0 33.0 39.0 21.5 41.0 72-73 32.74202677925284 35.0 33.0 39.0 20.5 40.0 74-75 32.53893650405552 35.0 33.0 38.5 20.0 40.0 76-77 32.19875907593688 35.0 33.0 37.5 19.5 39.5 78-79 31.81935155886411 35.0 32.5 37.0 18.0 39.0 80-81 31.48353575413249 35.0 32.0 36.5 15.5 39.0 82-83 31.14779660393856 35.0 32.0 36.0 11.0 38.5 84-85 30.828594788822492 35.0 32.0 36.0 7.5 37.0 86-87 30.53277462805317 35.0 31.5 35.0 4.5 37.0 88-89 30.248680432738833 35.0 31.0 35.0 2.0 36.5 90-91 29.984005386971916 34.5 31.0 35.0 2.0 36.0 92-93 29.733787426627543 34.0 31.0 35.0 2.0 36.0 94-95 29.468980820281992 34.0 31.0 35.0 2.0 36.0 96-97 29.209318941665067 34.0 31.0 35.0 2.0 35.0 98-99 28.891244086904614 34.0 30.0 35.0 2.0 35.0 100-101 28.348508370041678 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35772130783549017 1101 2 0.4124332164562716 1101 3 0.4114692160199205 1101 4 0.3647262107388656 1101 5 0.35092167861807155 1101 6 0.37739431748809693 1101 7 0.38141741578246524 1101 8 0.3762350943190427 1101 9 0.4486993674559727 1101 10-11 0.4184821308549118 1101 12-13 0.4396386249731776 1101 14-15 0.5292074573925163 1101 16-17 0.5049883239999673 1101 18-19 0.4678801833902355 1101 20-21 0.40397948034837583 1101 22-23 0.3934151116565303 1101 24-25 0.44394395819759325 1101 26-27 0.3930435403691135 1101 28-29 0.4249915246247724 1101 30-31 0.42093920531300455 1101 32-33 0.3701609282247773 1101 34-35 0.3535741612921086 1101 36-37 0.36280563887076767 1101 38-39 0.30539436402074926 1101 40-41 0.2583206998856937 1101 42-43 0.1945923067140427 1101 44-45 0.138602157354768 1101 46-47 0.14669110601280266 1101 48-49 0.0382131552345939 1101 50-51 -0.008558036266833824 1101 52-53 0.08358303375160858 1101 54-55 0.3461428357957814 1101 56-57 0.3550838587850649 1101 58-59 0.31639131178135216 1101 60-61 0.3216651535243713 1101 62-63 0.23428271610726625 1101 64-65 0.2279418114773364 1101 66-67 0.20529882956186185 1101 68-69 0.17090710147979138 1101 70-71 0.07217903262846903 1101 72-73 0.05476944117156535 1101 74-75 0.07247003134922636 1101 76-77 0.07425617036933829 1101 78-79 0.0030609076834551274 1101 80-81 -0.06834335379236833 1101 82-83 -0.14442284039506958 1101 84-85 -0.17269716622895004 1101 86-87 -0.23207052265616568 1101 88-89 -0.2854978350587771 1101 90-91 -0.3444889808457283 1101 92-93 -0.40749781644425553 1101 94-95 -0.5467679715570952 1101 96-97 -0.6514127044398457 1101 98-99 -0.8093361023684373 1101 100-101 -0.891217905344245 1102 1 0.31291888876859275 1102 2 0.3589007754085287 1102 3 0.3313977709094722 1102 4 0.32898911473870385 1102 5 0.3072409764351747 1102 6 0.35229650919825417 1102 7 0.35238024462618966 1102 8 0.35931845447457533 1102 9 0.44721908154927803 1102 10-11 0.44918648003291395 1102 12-13 0.40250265386782047 1102 14-15 0.5515434087936413 1102 16-17 0.45200653722366724 1102 18-19 0.4511338513545411 1102 20-21 0.3903898452282064 1102 22-23 0.4039664600543702 1102 24-25 0.38844245619495155 1102 26-27 0.40569257757425703 1102 28-29 0.4274472766300619 1102 30-31 0.38985493456507925 1102 32-33 0.401022676564871 1102 34-35 0.37005534001314544 1102 36-37 0.3335181721577385 1102 38-39 0.3156071673102119 1102 40-41 0.33164954877945974 1102 42-43 0.2733236400623227 1102 44-45 0.3454176085165557 1102 46-47 0.2612186628980453 1102 48-49 0.18663160861577666 1102 50-51 0.15060133512604068 1102 52-53 0.3175061438630138 1102 54-55 0.47968818791816403 1102 56-57 0.46087811594532724 1102 58-59 0.4604913642333557 1102 60-61 0.5510884807087635 1102 62-63 0.48785709609362726 1102 64-65 0.40050416867121896 1102 66-67 0.4000612736388973 1102 68-69 0.3554479274286919 1102 70-71 0.3509044673861581 1102 72-73 0.3143379586629962 1102 74-75 0.30991914437142043 1102 76-77 0.30978741864194603 1102 78-79 0.27736999361449577 1102 80-81 0.22391695887420582 1102 82-83 0.1796058271058989 1102 84-85 0.14763819852340987 1102 86-87 0.13004970650967707 1102 88-89 0.10896535907132687 1102 90-91 0.04318116273861605 1102 92-93 -0.097505076580088 1102 94-95 -0.14568968275967364 1102 96-97 -0.19415914163313275 1102 98-99 -0.28930621257914524 1102 100-101 -0.4079190773495469 1103 1 0.28858935903657823 1103 2 0.2880264991512931 1103 3 0.3497227664128566 1103 4 0.3232769598143861 1103 5 0.3137434182144787 1103 6 0.3114141563703612 1103 7 0.3297861055897826 1103 8 0.3268098531386485 1103 9 0.38522548965526937 1103 10-11 0.4443750662247936 1103 12-13 0.3908477810998079 1103 14-15 0.42933238370337534 1103 16-17 0.48887771277616565 1103 18-19 0.46373989405701366 1103 20-21 0.4179125731807787 1103 22-23 0.421424453477826 1103 24-25 0.4737064442543044 1103 26-27 0.49310884140735567 1103 28-29 0.48495424429309253 1103 30-31 0.5023912524936236 1103 32-33 0.42069841464159197 1103 34-35 0.42738687239062045 1103 36-37 0.4319280531762928 1103 38-39 0.39931845359556206 1103 40-41 0.45753263403201316 1103 42-43 0.4248914268316142 1103 44-45 0.4129656754097155 1103 46-47 0.32512161078857105 1103 48-49 0.2959571526994651 1103 50-51 0.2549798673418806 1103 52-53 0.37779279472060523 1103 54-55 0.543616459343319 1103 56-57 0.5881035603653331 1103 58-59 0.5728510400767064 1103 60-61 0.5773108884871334 1103 62-63 0.6043533665050731 1103 64-65 0.5445006165720727 1103 66-67 0.5618723242516879 1103 68-69 0.5206982478404072 1103 70-71 0.5611495927822716 1103 72-73 0.5945789706697511 1103 74-75 0.5319681245166166 1103 76-77 0.4673338704777379 1103 78-79 0.443371444084935 1103 80-81 0.45443866733786464 1103 82-83 0.45087997128276314 1103 84-85 0.419621619921017 1103 86-87 0.35857927123887734 1103 88-89 0.318105133042895 1103 90-91 0.29575874551127157 1103 92-93 0.2799137318028606 1103 94-95 0.2222140947413962 1103 96-97 0.17558462656274187 1103 98-99 0.19509065785563706 1103 100-101 0.171703302210247 1104 1 0.14380064259837866 1104 2 0.15041381728235237 1104 3 0.19565165343158242 1104 4 0.14870381572725222 1104 5 0.13578860425082695 1104 6 0.10697566408208559 1104 7 0.09925154442193218 1104 8 0.14261040016157978 1104 9 0.08858935701029935 1104 10-11 0.14298849256281443 1104 12-13 0.15006948722873403 1104 14-15 0.15408759903296243 1104 16-17 0.19464168370950574 1104 18-19 0.15444171800625384 1104 20-21 0.148003285119799 1104 22-23 0.1430427836013095 1104 24-25 0.18549009506594416 1104 26-27 0.1697689265357809 1104 28-29 0.1697460357122793 1104 30-31 0.13228006765488942 1104 32-33 0.1357407360903906 1104 34-35 0.10871769179212976 1104 36-37 0.1072056106703414 1104 38-39 0.08978156191582087 1104 40-41 0.011621549534773123 1104 42-43 -0.005070216826645435 1104 44-45 -6.787434997974628E-4 1104 46-47 0.03923820112018461 1104 48-49 0.04292505960845716 1104 50-51 -0.07042271240532472 1104 52-53 0.02354729794933519 1104 54-55 0.14781340378327457 1104 56-57 0.07902953424417802 1104 58-59 0.13238145811227753 1104 60-61 0.048904942444956134 1104 62-63 0.11057372935282928 1104 64-65 0.08565519101088626 1104 66-67 0.13367029800106422 1104 68-69 0.037221415394505186 1104 70-71 0.04584711175814249 1104 72-73 0.014408147384052938 1104 74-75 0.09003023913248853 1104 76-77 0.09234115332743897 1104 78-79 0.038142358001653776 1104 80-81 0.05742706333067815 1104 82-83 0.03586718583551729 1104 84-85 0.03353995489973727 1104 86-87 0.015828538907882717 1104 88-89 -0.049967663085865865 1104 90-91 -0.07093030917483745 1104 92-93 -0.11187849658730897 1104 94-95 -0.12690751266445233 1104 96-97 -0.13925701191659812 1104 98-99 -0.13460111576102207 1104 100-101 -0.1964423224154963 1105 1 0.27910033447479776 1105 2 0.26057398956008626 1105 3 0.27583299942839545 1105 4 0.2819940619782315 1105 5 0.3224387283979411 1105 6 0.3297715504409737 1105 7 0.3050048612087366 1105 8 0.33078062550357146 1105 9 0.3630914268508434 1105 10-11 0.3743170953019188 1105 12-13 0.29744117119400926 1105 14-15 0.4351433657186945 1105 16-17 0.4151143331992557 1105 18-19 0.42065152000864714 1105 20-21 0.42564030119935836 1105 22-23 0.3555675691024831 1105 24-25 0.39660821086967246 1105 26-27 0.43505047707389366 1105 28-29 0.4261132652064674 1105 30-31 0.4284585458858885 1105 32-33 0.36871058483956887 1105 34-35 0.41978856502874606 1105 36-37 0.37570376512971393 1105 38-39 0.38652172250203876 1105 40-41 0.4367850153149604 1105 42-43 0.40579500804725654 1105 44-45 0.40896454623865 1105 46-47 0.3608010275974891 1105 48-49 0.32440726085698657 1105 50-51 0.2687187171119163 1105 52-53 0.3980853652782983 1105 54-55 0.5144000272296907 1105 56-57 0.5149794973141155 1105 58-59 0.5216900837193847 1105 60-61 0.5226865390704418 1105 62-63 0.5254932834195785 1105 64-65 0.47323907183736225 1105 66-67 0.4652873980775709 1105 68-69 0.4854273410034651 1105 70-71 0.48526203915789523 1105 72-73 0.5206364348758896 1105 74-75 0.49487048440877146 1105 76-77 0.4888255470353897 1105 78-79 0.520554140389347 1105 80-81 0.48214268738380994 1105 82-83 0.4783769827117297 1105 84-85 0.4765818082523552 1105 86-87 0.43475768853445373 1105 88-89 0.4728935879252276 1105 90-91 0.425010452063443 1105 92-93 0.43653068276622164 1105 94-95 0.4515181871476628 1105 96-97 0.4037188269879337 1105 98-99 0.46405425801480504 1105 100-101 0.47255507382834594 1106 1 0.25227565613742087 1106 2 0.2627579812396874 1106 3 0.25479237966028734 1106 4 0.2543046841752812 1106 5 0.24489491176890965 1106 6 0.28227457383135146 1106 7 0.2997614286836878 1106 8 0.27583060863540254 1106 9 0.3266602583358207 1106 10-11 0.3731262313692696 1106 12-13 0.3504942080259852 1106 14-15 0.43433665038628533 1106 16-17 0.4083064296293628 1106 18-19 0.4054170620258404 1106 20-21 0.41713218410483677 1106 22-23 0.46976021634319665 1106 24-25 0.4304474493697086 1106 26-27 0.46291040449349197 1106 28-29 0.429711440339247 1106 30-31 0.49758271777377416 1106 32-33 0.4964876586616995 1106 34-35 0.47753762954604184 1106 36-37 0.4773594866740467 1106 38-39 0.4662269040895879 1106 40-41 0.5419259449564109 1106 42-43 0.5520827831751873 1106 44-45 0.593895664574525 1106 46-47 0.6221873546331125 1106 48-49 0.5309976361809561 1106 50-51 0.4802460157288948 1106 52-53 0.6182846618659781 1106 54-55 0.6664002537645999 1106 56-57 0.6537309443147876 1106 58-59 0.6668167841366639 1106 60-61 0.6441684557485488 1106 62-63 0.6801484420175186 1106 64-65 0.7371232556254697 1106 66-67 0.7169194577885563 1106 68-69 0.7670245769205977 1106 70-71 0.7675958228840329 1106 72-73 0.7399949407115756 1106 74-75 0.7481814426603748 1106 76-77 0.7334784724083789 1106 78-79 0.757142613248373 1106 80-81 0.7404837676995406 1106 82-83 0.734556701043406 1106 84-85 0.7507119674318048 1106 86-87 0.7202343318510103 1106 88-89 0.7579752615542148 1106 90-91 0.6965900024332825 1106 92-93 0.7014225989023792 1106 94-95 0.6739214756874006 1106 96-97 0.6693593315492379 1106 98-99 0.6777358750933473 1106 100-101 0.680211588006916 1107 1 0.2623243669360562 1107 2 0.27261575709226804 1107 3 0.24649012835643802 1107 4 0.2567158756496113 1107 5 0.25456819121978214 1107 6 0.2593131106886375 1107 7 0.2520532554462349 1107 8 0.31150377146359176 1107 9 0.39468472896945883 1107 10-11 0.33443002445284975 1107 12-13 0.34166055120351757 1107 14-15 0.44466220991488825 1107 16-17 0.36117083562071883 1107 18-19 0.3017136321386431 1107 20-21 0.38924569442698953 1107 22-23 0.39737023909155766 1107 24-25 0.38359515998993743 1107 26-27 0.3947730337242703 1107 28-29 0.40641623151869055 1107 30-31 0.4079784418167236 1107 32-33 0.45570608358896436 1107 34-35 0.4478220813118057 1107 36-37 0.4595833685713586 1107 38-39 0.47220903200939546 1107 40-41 0.41955374745867147 1107 42-43 0.4419374681793826 1107 44-45 0.4878057647936771 1107 46-47 0.44936439828957475 1107 48-49 0.3871617614448297 1107 50-51 0.34663170327866055 1107 52-53 0.42450990123752064 1107 54-55 0.4681027628184822 1107 56-57 0.4516218090789792 1107 58-59 0.4485284893755903 1107 60-61 0.5480824399197886 1107 62-63 0.5750177985726523 1107 64-65 0.5381512967877313 1107 66-67 0.5571077409567735 1107 68-69 0.5639922221071672 1107 70-71 0.574528217474132 1107 72-73 0.5691218307106922 1107 74-75 0.570914927533785 1107 76-77 0.5528940417457733 1107 78-79 0.590148795639319 1107 80-81 0.5864264000957995 1107 82-83 0.5372447672795424 1107 84-85 0.6267460566606573 1107 86-87 0.6132829947510423 1107 88-89 0.6166043861273778 1107 90-91 0.6073840398205768 1107 92-93 0.5763072517728567 1107 94-95 0.6237557222180499 1107 96-97 0.6471774008467186 1107 98-99 0.6694128243409523 1107 100-101 0.6915984574026908 1108 1 0.1367078644230837 1108 2 0.14761282746449922 1108 3 0.13836068942071833 1108 4 0.1688171544095951 1108 5 0.1896475903983088 1108 6 0.1710185544141254 1108 7 0.17148497955317765 1108 8 0.21999524313577012 1108 9 0.17686596447165215 1108 10-11 0.21600702780047953 1108 12-13 0.22745008170983994 1108 14-15 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-1.2587532300258815 2316 56-57 -1.213069604158946 2316 58-59 -1.1126670979827864 2316 60-61 -1.1630460633598716 2316 62-63 -1.2929756420952074 2316 64-65 -1.2456143561639692 2316 66-67 -1.1082545130682675 2316 68-69 -1.053777604964985 2316 70-71 -1.3027824563481758 2316 72-73 -1.261677215204628 2316 74-75 -1.0314255614346095 2316 76-77 -0.9542243243842208 2316 78-79 -0.9894576444322389 2316 80-81 -0.9199838563144596 2316 82-83 -0.8188971528158682 2316 84-85 -0.9480526911032108 2316 86-87 -0.8658091340503908 2316 88-89 -0.9122790366513911 2316 90-91 -0.8538562702569443 2316 92-93 -0.9207485025503388 2316 94-95 -1.1894896564343043 2316 96-97 -0.9373858072630021 2316 98-99 -0.7354023217532628 2316 100-101 -0.6778857405698773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96846.0 3 20701.0 4 16940.0 5 15492.0 6 16533.0 7 17808.0 8 19694.0 9 23571.0 10 29604.0 11 37290.0 12 48582.0 13 58979.0 14 63611.0 15 75087.0 16 92990.0 17 119932.0 18 95343.0 19 55896.0 20 50407.0 21 50414.0 22 52972.0 23 57404.0 24 65852.0 25 77391.0 26 92945.0 27 112742.0 28 138057.0 29 170652.0 30 216207.0 31 279723.0 32 370484.0 33 507307.0 34 736171.0 35 1142712.0 36 1779298.0 37 2627032.0 38 2589528.0 39 599047.0 40 318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.861302346810696 20.07231562131528 14.289934451586728 21.776447580287297 2 16.167473799832297 20.954881017021858 40.01572140796689 22.86192377517895 3 18.123741389790908 26.818895776140582 30.6677392650889 24.389623568979605 4 12.445080957304777 17.646719515722 38.999623638661426 30.908575888311802 5 12.313793635666686 37.56147190935387 35.07212302590274 15.052611429076702 6 28.831581286055947 38.25286054679849 18.69084701090212 14.224711156243444 7 25.841918833420475 33.273857519666834 23.234556736558932 17.64966691035376 8 25.426538480036214 34.22921831472861 21.3308182811974 19.013424924037782 9 25.5291486586026 16.454605732744902 22.350675167698444 35.66557044095405 10-11 23.42006742742329 26.91121818228351 29.11079441751202 20.557919972781182 12-13 24.65940628256483 24.94855888616444 29.186695755322184 21.205339075948544 14-15 22.100377287570332 26.57452471416612 27.555061974582014 23.770036023681534 16-17 20.445073474719884 29.689507530700716 27.99111786361246 21.87430113096694 18-19 20.397989649381884 27.737411895430203 31.273721222950616 20.5908772322373 20-21 21.815824001343742 27.168366417197376 30.807188708754772 20.20862087270411 22-23 21.35232299351183 27.254047283073312 30.987918960054444 20.405710763360414 24-25 20.831624829417024 27.23486772633923 31.12711895093541 20.80638849330833 26-27 20.630375319254362 27.249026187781126 31.11525292971624 21.005345563248266 28-29 20.70069851016929 27.19325712117973 31.263507775740855 20.842536592910125 30-31 21.195708237748942 27.8021471646515 30.57973302976624 20.42241156783332 32-33 20.559555465901784 27.539636271556056 31.08084178340364 20.819966479138515 34-35 20.917768265153125 27.17535263247467 30.95399971286762 20.952879389504584 36-37 21.43799845191384 27.539093256008517 30.27217959859494 20.750728693482703 38-39 21.162524306575516 27.832044608619817 30.417093606041046 20.588337478763624 40-41 20.92505605740684 27.399290884351174 30.63137708588798 21.044275972354004 42-43 20.840266916731913 27.64838192001665 30.310897752950495 21.200453410300945 44-45 21.152132153363908 27.842870821730127 29.99902263040466 21.00597439450131 46-47 21.38318930063979 28.18579425063115 29.425613836013508 21.005402612715553 48-49 21.26328249978409 28.04112296003543 29.468394959800904 21.227199580379576 50-51 21.31487344505224 28.059867162587093 29.084267911760563 21.540991480600105 52-53 21.303409555363654 28.11246472432477 29.03994933076258 21.544176389549 54-55 21.468353728290637 28.71884672335326 28.80626358769667 21.006535960659438 56-57 21.259385552221833 28.284698642984818 28.86948045779127 21.586435347002077 58-59 21.081291118305394 27.898254066750727 29.27982477002115 21.740630044922728 60-61 21.453979820372435 28.19013829100078 28.878041785009064 21.477840103617726 62-63 21.288332242320003 28.20502794721365 29.0287469731709 21.47789283729545 64-65 21.329480208451272 28.095926313601854 29.037479664290544 21.537113813656326 66-67 21.22304542340931 28.135051371470027 28.83632861751918 21.80557458760148 68-69 21.703463665559237 28.496604924858556 28.54257088406092 21.25736052552129 70-71 21.73908823538733 28.07095420101353 28.609229362566595 21.580728201032546 72-73 21.288362260126622 28.244131135500517 28.864463829112086 21.60304277526077 74-75 21.364165571268032 28.296835605586452 28.71580800368011 21.623190819465403 76-77 21.536303689398025 28.55145252672176 28.39237767109134 21.519866112788876 78-79 21.597879069331096 28.380125295883285 28.69296090561163 21.329034729173994 80-81 21.49581427914531 28.279691589390083 28.601939836587036 21.62255429487757 82-83 21.270697556993625 28.347415422576983 28.88776383438333 21.494123186046064 84-85 21.21002732826376 28.3134068787798 28.660375475503034 21.816190317453408 86-87 21.456167854182752 28.3962802233438 28.58118513219217 21.566366790281275 88-89 21.196066140293254 28.31277582934951 28.686581162250658 21.804576868106583 90-91 21.10731873162469 28.10511062443503 28.776377111652547 22.011193532287734 92-93 21.263612100817777 28.35846562570552 28.464230064950176 21.913692208526527 94-95 21.162858089661587 28.06687687838178 28.774056607417442 21.99620842453919 96-97 21.07092477519777 28.165878264992333 28.694242343666858 22.06895461614304 98-99 21.093417647519782 28.07087798638605 28.635884878617567 22.199819487476596 100-101 21.119712852164355 28.165656434552776 28.36252857986012 22.352102133422747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12237.0 1 7979.5 2 7056.0 3 9310.0 4 6326.5 5 4711.5 6 7054.0 7 12616.0 8 19399.5 9 22748.5 10 19718.0 11 15781.5 12 15366.5 13 16268.5 14 17199.5 15 18155.0 16 19051.5 17 20236.5 18 21841.0 19 23974.5 20 26902.5 21 31079.5 22 38428.5 23 50813.0 24 63879.0 25 77567.5 26 98171.5 27 125727.0 28 162623.5 29 197287.0 30 221360.0 31 250782.5 32 285594.0 33 315749.0 34 344161.5 35 371405.0 36 390286.0 37 405655.0 38 421794.5 39 429510.0 40 436987.5 41 438495.0 42 428258.5 43 421991.5 44 415937.0 45 409033.0 46 404106.0 47 399747.5 48 395289.0 49 392003.5 50 389725.0 51 373661.0 52 344485.0 53 318274.0 54 300563.5 55 291788.5 56 275578.0 57 249094.5 58 225279.0 59 204171.5 60 179052.0 61 151184.5 62 124491.0 63 99507.5 64 79812.5 65 64218.5 66 51109.0 67 39663.5 68 29739.0 69 21996.5 70 16326.0 71 12070.5 72 9091.5 73 6904.0 74 5313.5 75 3955.5 76 2862.5 77 2145.0 78 1636.5 79 1218.0 80 913.5 81 719.0 82 569.0 83 477.5 84 425.5 85 358.5 86 290.0 87 241.0 88 200.5 89 177.0 90 155.5 91 127.5 92 99.5 93 87.0 94 68.5 95 55.0 96 46.0 97 36.5 98 29.5 99 21.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014910991206952039 2 0.00439723704562082 3 0.003945628916611114 4 0.005641140137805448 5 0.002773032371112228 6 0.007114808769310803 7 0.0014657456818736065 8 0.0038426305713983736 9 0.0102206050249565 10-11 0.006980118625571066 12-13 0.0084260569333653 14-15 0.0033276388453346746 16-17 0.005740177008202313 18-19 0.0038030158232396274 20-21 8.081408624384209E-4 22-23 0.0010458293513908976 24-25 0.0069008891292535735 26-27 0.0012716334158957504 28-29 0.0010141375528639005 30-31 6.338359705399379E-4 32-33 0.0013310555381338695 34-35 0.00163212762414034 36-37 0.001358785861844992 38-39 0.001857931688645193 40-41 0.0027611479466646046 42-43 0.002555151256239125 44-45 0.007546609524241136 46-47 0.013801778258507147 48-49 0.005007304167265509 50-51 0.005704523734859441 52-53 0.0132788635828117 54-55 0.00791898815693335 56-57 0.005553987691856206 58-59 0.007055386647072685 60-61 0.0013429399625814935 62-63 0.0021233505013087918 64-65 0.002305578342839024 66-67 0.002420461112499388 68-69 0.002103543127229419 70-71 0.00255118978142325 72-73 0.001501398955216478 74-75 0.0016281661493244657 76-77 0.00862413067415903 78-79 0.00694050387741232 80-81 0.01293817674864648 82-83 0.01526356246556488 84-85 0.008806358515689262 86-87 0.005946173698627793 88-89 0.0033316003201505485 90-91 0.0027334176229534823 92-93 9.309465817305338E-4 94-95 0.0012637104662640013 96-97 0.0019490456094103093 98-99 0.0065839711439836055 100-101 0.011428854843798256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2621562E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.31182235071484 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89097065048053 38.13059507639107 2 15.963375083228629 16.701464829433675 3 5.247361824297204 8.234971786876748 4 2.081942897976812 4.356409080931818 5 1.0741467722582605 2.8095287564483944 6 0.642181178733765 2.015620064333602 7 0.40441716636999503 1.4809059273908667 8 0.2815362500962176 1.1782139440255808 9 0.20084123522358144 0.9455733915942713 >10 1.0653872730483742 10.185027815568573 >50 0.08300515753342164 3.01898013523233 >100 0.05780281417330908 5.855980576301723 >500 0.004790679035916865 1.6853872695845036 >1k 0.0020896004407938646 1.8918149509219995 >5k 6.056684128644745E-5 0.2134683790859241 >10k+ 9.085026192967118E-5 1.2960580158788249 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55256 0.43779050485193516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46175 0.3658421992460204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26587 0.21064746185931663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 7.922949631749224E-6 0.0 1.584589926349845E-5 0.0 4 0.0 7.922949631749224E-6 0.0 3.16917985269969E-5 0.0 5 0.0 2.3768848895247672E-5 0.0 3.961474815874612E-5 0.0 6 0.0 2.3768848895247672E-5 0.0 3.961474815874612E-5 0.0 7 0.0 2.3768848895247672E-5 0.0 3.961474815874612E-5 0.0 8 0.0 2.3768848895247672E-5 0.0 3.961474815874612E-5 0.0 9 1.584589926349845E-5 2.3768848895247672E-5 0.0 5.5460647422244566E-5 0.0 10-11 1.980737407937306E-5 2.3768848895247672E-5 0.0 8.715244594924146E-5 0.0 12-13 2.3768848895247672E-5 2.3768848895247672E-5 0.0 1.386516185556114E-4 3.961474815874612E-6 14-15 2.3768848895247672E-5 2.7730323711122287E-5 0.0 1.9015079116198137E-4 7.922949631749224E-6 16-17 2.3768848895247672E-5 3.961474815874612E-5 0.0 2.2184258968897827E-4 7.922949631749224E-6 18-19 3.16917985269969E-5 4.7537697790495344E-5 0.0 2.3372701413660208E-4 7.922949631749224E-6 20-21 3.961474815874612E-5 4.7537697790495344E-5 0.0 2.971106111905959E-4 7.922949631749224E-6 22-23 3.961474815874612E-5 4.7537697790495344E-5 0.0 4.832999275367027E-4 7.922949631749224E-6 24-25 3.961474815874612E-5 4.7537697790495344E-5 0.0 8.160638120701701E-4 1.584589926349845E-5 26-27 3.961474815874612E-5 4.7537697790495344E-5 0.0 0.0012993637396068727 1.584589926349845E-5 28-29 3.961474815874612E-5 5.5460647422244566E-5 0.0 0.002594766004397871 1.584589926349845E-5 30-31 3.961474815874612E-5 5.5460647422244566E-5 0.0 0.006005595820865912 1.584589926349845E-5 32-33 3.961474815874612E-5 5.5460647422244566E-5 0.0 0.013001560345700477 1.584589926349845E-5 34-35 4.7537697790495344E-5 5.5460647422244566E-5 0.0 0.0239827685353049 1.584589926349845E-5 36-37 4.7537697790495344E-5 5.5460647422244566E-5 0.0 0.0418173281563724 1.584589926349845E-5 38-39 4.7537697790495344E-5 7.526802150161762E-5 0.0 0.06622793597179176 1.584589926349845E-5 40-41 4.7537697790495344E-5 8.715244594924146E-5 0.0 0.09321350241752963 1.584589926349845E-5 42-43 4.7537697790495344E-5 8.715244594924146E-5 0.0 0.12145485637989974 1.584589926349845E-5 44-45 4.7537697790495344E-5 8.715244594924146E-5 0.0 0.1555512701201325 1.584589926349845E-5 46-47 5.5460647422244566E-5 8.715244594924146E-5 0.0 0.19691302867267935 1.584589926349845E-5 48-49 5.5460647422244566E-5 8.715244594924146E-5 0.0 0.2414201982290306 1.584589926349845E-5 50-51 5.5460647422244566E-5 1.1884424447623836E-4 0.0 0.28795960436592555 1.584589926349845E-5 52-53 5.5460647422244566E-5 1.307286689238622E-4 0.0 0.3342256687405252 1.584589926349845E-5 54-55 5.5460647422244566E-5 1.4261309337148604E-4 0.0 0.3877848082511499 1.584589926349845E-5 56-57 6.33835970539938E-5 1.5053604300323525E-4 0.0 0.4501938825004385 1.584589926349845E-5 58-59 7.130654668574302E-5 1.5845899263498448E-4 0.0 0.5196424974975363 1.584589926349845E-5 60-61 7.130654668574302E-5 1.5845899263498448E-4 0.0 0.5938290363744202 1.584589926349845E-5 62-63 1.267671941079876E-4 1.5845899263498448E-4 0.0 0.6701310028029812 1.584589926349845E-5 64-65 1.267671941079876E-4 1.5845899263498448E-4 0.0 0.7543836491870024 1.584589926349845E-5 66-67 1.267671941079876E-4 1.703434170826083E-4 0.0 0.85372555314469 1.584589926349845E-5 68-69 1.267671941079876E-4 1.7430489189848293E-4 0.0 0.9669761951809135 1.584589926349845E-5 70-71 1.267671941079876E-4 1.7430489189848293E-4 0.0 1.0920676854417861 1.584589926349845E-5 72-73 1.307286689238622E-4 1.7430489189848293E-4 0.0 1.2305370761558672 2.3768848895247672E-5 74-75 1.4261309337148604E-4 1.8222784153023217E-4 0.0 1.3746000693099634 2.3768848895247672E-5 76-77 1.4261309337148604E-4 1.8222784153023217E-4 0.0 1.5293669674165526 2.3768848895247672E-5 78-79 1.4261309337148604E-4 1.8222784153023217E-4 0.0 1.70663504247731 2.3768848895247672E-5 80-81 1.4261309337148604E-4 1.8222784153023217E-4 0.0 1.9045503242784054 2.3768848895247672E-5 82-83 1.4261309337148604E-4 1.8222784153023217E-4 0.0 2.116271345812824 2.3768848895247672E-5 84-85 1.4261309337148604E-4 1.9411226597785598E-4 0.0 2.3318825356164314 2.3768848895247672E-5 86-87 1.4261309337148604E-4 2.0599669042547982E-4 0.0 2.565375822738897 2.3768848895247672E-5 88-89 1.4261309337148604E-4 2.1788111487310366E-4 0.0 2.8322049204369475 2.3768848895247672E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 27095 0.0 58.785107 1 GTATCAA 50630 0.0 42.305344 1 TATAACG 1400 0.0 35.963436 2 TACCTGG 11495 0.0 34.048782 2 GTGGTAT 10400 0.0 33.891712 1 ATCAACG 62690 0.0 33.779324 3 TCAACGC 63415 0.0 33.476727 4 GTACCTG 12085 0.0 33.09698 1 TATCAAC 64820 0.0 32.82127 2 CAACGCA 66200 0.0 32.110542 5 TGGTATC 10780 0.0 31.504358 2 AACGCAG 70120 0.0 30.579752 6 ATAACGC 1855 0.0 27.399902 3 CGCAGAG 77675 0.0 27.24023 8 ACGCAGA 78755 0.0 27.089968 7 CCCGAGG 4570 0.0 26.71321 3 CCGAGGG 4765 0.0 25.32185 4 GTACATG 49895 0.0 24.667965 1 TACATGG 49205 0.0 24.02697 2 GCAGAGT 86475 0.0 23.808947 9 >>END_MODULE