FastQCFastQC Report
Wed 25 May 2016
SRR2049428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12621562
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518670.41093962854993704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442930.35093120803906835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292800.23198396521761727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA261300.058.41231
GTATCAA486650.041.9386671
TATAACG14100.035.0338552
TACCTGG110600.032.3809782
ATCAACG623450.032.3781783
TCAACGC631350.031.988084
TATCAAC638900.031.6627372
GTACCTG114050.031.5821781
CAACGCA653600.030.9354695
GTGGTAT107650.030.0167061
AACGCAG699700.029.1003466
TGGTATC106050.028.9779262
ATAACGC18800.028.8013023
CGCAGAG792200.025.432298
ACGCAGA802150.025.282517
GTACATG486800.023.6912171
TACATGG477800.023.0331652
CCGAGGG40100.022.9785444
GCAGAGT886750.022.32439
ACCTGGG159450.022.072873
Produced by FastQC (version 0.11.5)