##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12621562 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26549257532467 33.0 31.0 34.0 31.0 34.0 2 32.43897395583843 34.0 31.0 34.0 31.0 34.0 3 32.51120566535267 34.0 31.0 34.0 31.0 34.0 4 36.11333977521958 37.0 35.0 37.0 35.0 37.0 5 36.03234987872341 37.0 35.0 37.0 35.0 37.0 6 36.07224177166027 37.0 35.0 37.0 35.0 37.0 7 36.010351096005394 37.0 35.0 37.0 35.0 37.0 8 36.00483553461925 37.0 35.0 37.0 35.0 37.0 9 37.660058556936136 39.0 37.0 39.0 35.0 39.0 10-11 37.65692419844707 39.0 37.0 39.0 35.0 39.0 12-13 37.64986358265324 39.0 37.0 39.0 35.0 39.0 14-15 38.927434536232525 40.0 38.0 41.0 36.0 41.0 16-17 38.91197559382904 40.0 38.0 41.0 36.0 41.0 18-19 38.94593430670467 40.0 38.0 41.0 35.5 41.0 20-21 38.9353804624182 40.0 38.0 41.0 35.0 41.0 22-23 38.84610426982017 40.0 38.0 41.0 35.0 41.0 24-25 38.74642508589666 40.0 38.0 41.0 35.0 41.0 26-27 38.58915655605859 40.0 38.0 41.0 34.5 41.0 28-29 38.41152216342161 40.0 38.0 41.0 34.0 41.0 30-31 38.219564662440355 40.0 38.0 41.0 34.0 41.0 32-33 37.956998745480156 40.0 37.0 41.0 33.5 41.0 34-35 37.77282514636461 40.0 37.0 41.0 33.0 41.0 36-37 37.574360091088565 40.0 37.0 41.0 33.0 41.0 38-39 37.71976067621424 40.0 37.0 41.0 33.0 41.0 40-41 37.62402890387101 40.0 37.0 41.0 33.0 41.0 42-43 37.43249821218642 40.0 37.0 41.0 33.0 41.0 44-45 37.1896436827708 40.0 37.0 41.0 32.5 41.0 46-47 36.84573430768712 40.0 37.0 41.0 31.0 41.0 48-49 36.558247901487945 40.0 36.0 41.0 31.0 41.0 50-51 36.330298183378574 39.5 36.0 41.0 30.0 41.0 52-53 36.14929495255817 39.0 35.5 41.0 30.0 41.0 54-55 36.037838700154545 39.0 35.0 41.0 30.0 41.0 56-57 35.93688106907845 39.0 35.0 41.0 29.5 41.0 58-59 35.70494884072193 39.0 35.0 40.5 29.0 41.0 60-61 35.45850838430299 38.5 35.0 40.0 28.5 41.0 62-63 35.17038703292033 38.0 35.0 40.0 28.0 41.0 64-65 34.91024019055645 38.0 34.5 40.0 28.0 41.0 66-67 34.57529155266202 37.0 34.0 40.0 27.5 41.0 68-69 34.248119289831166 36.5 34.0 39.5 26.5 41.0 70-71 33.91332348563513 36.0 34.0 39.0 26.0 41.0 72-73 33.54829980631557 36.0 34.0 39.0 26.0 40.0 74-75 33.18349995032311 35.0 33.0 38.0 26.0 40.0 76-77 31.17530631311719 33.5 30.5 35.5 23.0 38.5 78-79 32.471681397278715 35.0 33.0 37.0 25.5 39.0 80-81 32.47392228473781 35.0 33.0 36.5 25.5 39.0 82-83 32.210255275852546 35.0 33.0 36.0 25.0 38.5 84-85 31.95176551840414 35.0 33.0 36.0 25.0 37.0 86-87 31.695097722453053 35.0 33.0 35.5 24.0 37.0 88-89 31.449442668031104 35.0 33.0 35.0 24.0 36.5 90-91 31.20137673926571 35.0 32.5 35.0 23.0 36.0 92-93 30.978453380017466 35.0 32.0 35.0 20.5 36.0 94-95 30.741965455622687 35.0 32.0 35.0 19.5 36.0 96-97 30.532680305337802 35.0 32.0 35.0 17.0 35.0 98-99 30.279802808875793 34.0 32.0 35.0 4.5 35.0 100-101 29.702905551626653 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3920402463434982 1101 2 0.36531008771837037 1101 3 0.3702064905314586 1101 4 0.27127491186371344 1101 5 0.30952257187781385 1101 6 0.3073456044480949 1101 7 0.3195584494386736 1101 8 0.34047636916304214 1101 9 0.4038946402390948 1101 10-11 0.3622022460398284 1101 12-13 0.290278037966722 1101 14-15 0.3998393502053119 1101 16-17 0.4164533755357738 1101 18-19 0.35341817280181687 1101 20-21 0.3558050960267849 1101 22-23 0.305050504853547 1101 24-25 0.24237682281346196 1101 26-27 0.2536652452712005 1101 28-29 0.24697041824685328 1101 30-31 0.18510317408074428 1101 32-33 0.24025163092783686 1101 34-35 0.20300575962341583 1101 36-37 0.18935423436651178 1101 38-39 0.33781899278856997 1101 40-41 0.31782921669691433 1101 42-43 0.36471657621554243 1101 44-45 0.34703759379352306 1101 46-47 0.3699081031125644 1101 48-49 0.24942595156196745 1101 50-51 0.3191064576299212 1101 52-53 0.26864929492213463 1101 54-55 0.19018108700719694 1101 56-57 0.17119224367139907 1101 58-59 0.16796289847087564 1101 60-61 0.1622317328797962 1101 62-63 0.17483257784572714 1101 64-65 0.11117935654976208 1101 66-67 0.0619726575685462 1101 68-69 0.027536232330199084 1101 70-71 -0.026667147756207044 1101 72-73 -0.030243503920267756 1101 74-75 -0.14655862979370937 1101 76-77 -0.19582433656826836 1101 78-79 -0.13450114421096515 1101 80-81 -0.08143268518571745 1101 82-83 -0.10893816084600871 1101 84-85 -0.1438653170879931 1101 86-87 -0.1973838982460272 1101 88-89 -0.2746922461393737 1101 90-91 -0.19396006762933027 1101 92-93 -0.2592397123321959 1101 94-95 -0.26948003152902444 1101 96-97 -0.37696383886320817 1101 98-99 -0.45560488609922345 1101 100-101 -0.47303095494804026 1102 1 0.33922749269769525 1102 2 0.365852075526405 1102 3 0.3298798243455252 1102 4 0.264347851490605 1102 5 0.2516557930502543 1102 6 0.2684277882306958 1102 7 0.2707445436455771 1102 8 0.28538095672881525 1102 9 0.363989980239225 1102 10-11 0.30867716305557735 1102 12-13 0.27276725641412725 1102 14-15 0.3532782460539039 1102 16-17 0.35925043431748804 1102 18-19 0.324920925688744 1102 20-21 0.3086601487583991 1102 22-23 0.29655633881997545 1102 24-25 0.31469682792188536 1102 26-27 0.24858948311543116 1102 28-29 0.2306544486214861 1102 30-31 0.18078198774347953 1102 32-33 0.2263716389336281 1102 34-35 0.22979139032761253 1102 36-37 0.157009367501324 1102 38-39 0.2711763777497964 1102 40-41 0.2930699130494787 1102 42-43 0.3844630827675104 1102 44-45 0.34715272624257665 1102 46-47 0.32580942110831757 1102 48-49 0.16240384766945226 1102 50-51 0.1455547342743344 1102 52-53 0.22013970676515981 1102 54-55 0.2450032866530023 1102 56-57 0.2293579986261065 1102 58-59 0.19945188294398974 1102 60-61 0.22027913159595158 1102 62-63 0.2251094096413624 1102 64-65 0.2024384101039729 1102 66-67 0.12179120489015105 1102 68-69 0.08181056985696245 1102 70-71 0.025265153905138504 1102 72-73 4.6439931033148696E-5 1102 74-75 -0.010409777639473816 1102 76-77 -0.040112066175257866 1102 78-79 0.0402038055360876 1102 80-81 0.07721132501203698 1102 82-83 0.08599445615320178 1102 84-85 0.046695295051090824 1102 86-87 0.0034016569756367687 1102 88-89 -0.04954213962078313 1102 90-91 -0.08312557744115523 1102 92-93 -0.05725911587610355 1102 94-95 -0.15497036734996783 1102 96-97 -0.14360305071330615 1102 98-99 -0.246866127513929 1102 100-101 -0.26826501784787027 1103 1 0.34226354378229473 1103 2 0.3232382733591237 1103 3 0.2845045436843776 1103 4 0.2276301769986162 1103 5 0.25295968868930174 1103 6 0.24964952392437567 1103 7 0.234554535869421 1103 8 0.23939983698052458 1103 9 0.3595167206721612 1103 10-11 0.2867410058368023 1103 12-13 0.2629315185325467 1103 14-15 0.37680134477640337 1103 16-17 0.3557222272378624 1103 18-19 0.3181608386091668 1103 20-21 0.27365424250130843 1103 22-23 0.24038464358617517 1103 24-25 0.26445443451471107 1103 26-27 0.24446321695474893 1103 28-29 0.2228208811818604 1103 30-31 0.2055718152443049 1103 32-33 0.23018389968400044 1103 34-35 0.16626568286380206 1103 36-37 0.14710290987803631 1103 38-39 0.21522686202949615 1103 40-41 0.2973429337472524 1103 42-43 0.37961109353165057 1103 44-45 0.40637019114942063 1103 46-47 0.4307320723135746 1103 48-49 0.42771626690856124 1103 50-51 0.45999318665909783 1103 52-53 0.3952626494767131 1103 54-55 0.37202591170665045 1103 56-57 0.33571545755287957 1103 58-59 0.3337316488813542 1103 60-61 0.3642556223690008 1103 62-63 0.31420089750590563 1103 64-65 0.31327994486037625 1103 66-67 0.2833876120679335 1103 68-69 0.2945796909871703 1103 70-71 0.3176365177312164 1103 72-73 0.3486573493739442 1103 74-75 0.34044068871796895 1103 76-77 0.2751706773475995 1103 78-79 0.3660705091256844 1103 80-81 0.3197595427551505 1103 82-83 0.44268575565428847 1103 84-85 0.39927526574640737 1103 86-87 0.36967031727528266 1103 88-89 0.36107001975841513 1103 90-91 0.36872277098295214 1103 92-93 0.3084958329331222 1103 94-95 0.2908753533321544 1103 96-97 0.2623148314509258 1103 98-99 0.28182510343067335 1103 100-101 0.24966668294234395 1104 1 0.3215818690505401 1104 2 0.30108222616625824 1104 3 0.2982488994758583 1104 4 0.22013369704263397 1104 5 0.23568881523286223 1104 6 0.2869611813391231 1104 7 0.2585304862813089 1104 8 0.25081853610173255 1104 9 0.367350639330823 1104 10-11 0.2921444757100744 1104 12-13 0.24428135426382624 1104 14-15 0.3288745293238122 1104 16-17 0.35946156595511525 1104 18-19 0.3001418546476202 1104 20-21 0.3042523263026311 1104 22-23 0.2942955338740987 1104 24-25 0.30802651045055285 1104 26-27 0.2545358026900715 1104 28-29 0.29195940098312434 1104 30-31 0.30490583909968905 1104 32-33 0.3081989994590373 1104 34-35 0.29769886676385937 1104 36-37 0.3053928158483501 1104 38-39 0.37170374302773723 1104 40-41 0.36288777182522125 1104 42-43 0.30931257761457687 1104 44-45 0.3399720458484765 1104 46-47 0.43081420667405723 1104 48-49 0.3527834309668876 1104 50-51 0.3797309231494168 1104 52-53 0.3603838684639129 1104 54-55 0.3556637760514363 1104 56-57 0.29324471033250177 1104 58-59 0.32396939346372733 1104 60-61 0.3443147752926592 1104 62-63 0.3337933816050125 1104 64-65 0.3039376671086771 1104 66-67 0.31272252604939155 1104 68-69 0.28945418321070093 1104 70-71 0.2979566394357249 1104 72-73 0.3046887627323045 1104 74-75 0.2578406304551706 1104 76-77 0.20394643031333715 1104 78-79 0.32336711531772977 1104 80-81 0.2759519880123946 1104 82-83 0.2807540202321235 1104 84-85 0.23188462987270597 1104 86-87 0.1564503266620676 1104 88-89 0.16897798072234238 1104 90-91 0.14960529052526184 1104 92-93 0.09879912316277384 1104 94-95 0.0713459872637614 1104 96-97 0.07483728690415958 1104 98-99 0.11599825722827362 1104 100-101 0.1835068486676299 1105 1 0.28301682051199606 1105 2 0.28121132376102764 1105 3 0.25454838173293837 1105 4 0.2038974399246598 1105 5 0.21160219359143184 1105 6 0.19323071281944948 1105 7 0.21553295141433892 1105 8 0.2456786627252896 1105 9 0.2806364260567449 1105 10-11 0.24857506631917659 1105 12-13 0.23243885443650214 1105 14-15 0.29022473055596976 1105 16-17 0.26878940987703004 1105 18-19 0.3181266583286373 1105 20-21 0.301527413297066 1105 22-23 0.19230954106661358 1105 24-25 0.21135781537785903 1105 26-27 0.20668658493601555 1105 28-29 0.24538458336517976 1105 30-31 0.25867134605719855 1105 32-33 0.32418665550330417 1105 34-35 0.336113806437524 1105 36-37 0.24445986178723444 1105 38-39 0.3160398092741872 1105 40-41 0.3590476586741005 1105 42-43 0.3388818700422149 1105 44-45 0.3949639282132793 1105 46-47 0.3681642512332104 1105 48-49 0.27173333796184096 1105 50-51 0.2809032331328538 1105 52-53 0.3032314508983447 1105 54-55 0.29684768248468174 1105 56-57 0.32338824181907455 1105 58-59 0.33992817570774037 1105 60-61 0.3329172653104777 1105 62-63 0.3624722190287244 1105 64-65 0.40164773829501854 1105 66-67 0.3622227909013773 1105 68-69 0.30442217113701275 1105 70-71 0.25436004090055775 1105 72-73 0.3068127108150591 1105 74-75 0.31824078799457567 1105 76-77 0.34524163835752475 1105 78-79 0.3997949755808108 1105 80-81 0.3543960385414948 1105 82-83 0.45573514606847 1105 84-85 0.4273211615414674 1105 86-87 0.3977329780025407 1105 88-89 0.36381821035539375 1105 90-91 0.3366806940424887 1105 92-93 0.3586752383749534 1105 94-95 0.3451138479263456 1105 96-97 0.31401870293452916 1105 98-99 0.26293606406128944 1105 100-101 0.3920008721098611 1106 1 0.2673504083996576 1106 2 0.2457756833825826 1106 3 0.22079501293332982 1106 4 0.1998256830875249 1106 5 0.20923054319833767 1106 6 0.22836740801892574 1106 7 0.25008824666494434 1106 8 0.21814997122794466 1106 9 0.27400054743591085 1106 10-11 0.24206535836420784 1106 12-13 0.21799056123845872 1106 14-15 0.3083516877881678 1106 16-17 0.293869311322446 1106 18-19 0.2808861242368934 1106 20-21 0.24065247335886397 1106 22-23 0.22848159837780457 1106 24-25 0.25680079315548454 1106 26-27 0.26596066725396383 1106 28-29 0.32468309671197204 1106 30-31 0.30092076159058223 1106 32-33 0.26580128547060866 1106 34-35 0.28739261029512164 1106 36-37 0.3197372703848842 1106 38-39 0.37028298129597914 1106 40-41 0.35680613795380367 1106 42-43 0.3581171975193058 1106 44-45 0.31845287401748124 1106 46-47 0.3945959914395445 1106 48-49 0.41505563545180024 1106 50-51 0.47521133524798387 1106 52-53 0.4077277643817325 1106 54-55 0.3752020901681661 1106 56-57 0.3126840478119135 1106 58-59 0.399874712159658 1106 60-61 0.39763656160546645 1106 62-63 0.40582041217187026 1106 64-65 0.36862863886766917 1106 66-67 0.36485226753136857 1106 68-69 0.3471096354695149 1106 70-71 0.36262565022979487 1106 72-73 0.33843888228402363 1106 74-75 0.34402159428871926 1106 76-77 0.35068236387365204 1106 78-79 0.35395514528210725 1106 80-81 0.4109853162895263 1106 82-83 0.3631192740259479 1106 84-85 0.3544314743470345 1106 86-87 0.391851006142236 1106 88-89 0.38516514601560914 1106 90-91 0.3887427149944678 1106 92-93 0.3303638830328772 1106 94-95 0.4251452951126282 1106 96-97 0.4102238029326166 1106 98-99 0.44832947304943005 1106 100-101 0.5515735040035388 1107 1 0.22740038608168334 1107 2 0.2195067695077455 1107 3 0.2174681077316123 1107 4 0.16657382944622157 1107 5 0.19245046443636937 1107 6 0.17194925528068694 1107 7 0.17426923258483384 1107 8 0.1446875685089708 1107 9 0.1995573860724562 1107 10-11 0.19108725496846546 1107 12-13 0.19404815722830193 1107 14-15 0.18618243459683725 1107 16-17 0.2219239927863086 1107 18-19 0.17593636744378927 1107 20-21 0.196998118716202 1107 22-23 0.1965424702157108 1107 24-25 0.18168543734868337 1107 26-27 0.19012611215912045 1107 28-29 0.22522199940352294 1107 30-31 0.2413422293982066 1107 32-33 0.2850405410846335 1107 34-35 0.25035515289190613 1107 36-37 0.3070912143490787 1107 38-39 0.3311031199520258 1107 40-41 0.3341017046255317 1107 42-43 0.3335214114164273 1107 44-45 0.28949059208628825 1107 46-47 0.3160919134633957 1107 48-49 0.23614688368250114 1107 50-51 0.2542123525727007 1107 52-53 0.2838057953742137 1107 54-55 0.3547023897079882 1107 56-57 0.34264156209646046 1107 58-59 0.39029835796544887 1107 60-61 0.42965606744458995 1107 62-63 0.41409439288609207 1107 64-65 0.45927518673190804 1107 66-67 0.4549518775395356 1107 68-69 0.4520965657451157 1107 70-71 0.48988053779649476 1107 72-73 0.5074907779927926 1107 74-75 0.4684361119393614 1107 76-77 0.4695981650680636 1107 78-79 0.5819354711036908 1107 80-81 0.5863687307381085 1107 82-83 0.5962439013366208 1107 84-85 0.5696798910898941 1107 86-87 0.5243348507758263 1107 88-89 0.46170612743719985 1107 90-91 0.5169449276323412 1107 92-93 0.502262700006753 1107 94-95 0.5806498340260795 1107 96-97 0.5695840407040684 1107 98-99 0.6104826073189393 1107 100-101 0.7410327391669469 1108 1 0.13528947571695227 1108 2 0.13044280943457665 1108 3 0.08840092144021838 1108 4 0.11901814925964516 1108 5 0.10022678813673735 1108 6 0.13055820447674193 1108 7 0.13426035597759522 1108 8 0.11463480926570924 1108 9 0.21884166360777613 1108 10-11 0.1290257454932089 1108 12-13 0.10552422429108077 1108 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11 20022.0 12 31342.0 13 45077.0 14 52870.0 15 65546.0 16 83879.0 17 114615.0 18 96219.0 19 52715.0 20 40385.0 21 37978.0 22 38404.0 23 41446.0 24 47368.0 25 57056.0 26 71005.0 27 89578.0 28 115531.0 29 148489.0 30 192306.0 31 253182.0 32 339517.0 33 473018.0 34 696032.0 35 1096972.0 36 1779697.0 37 2828214.0 38 2976697.0 39 711267.0 40 326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.63846167016819 21.409727185273166 15.368808146963945 22.583002997594694 2 15.386943563987343 21.823633647445565 39.273726882801554 23.515695905765536 3 17.159661299570725 27.059469856959577 31.28236283742622 24.49850600604348 4 12.34087495008525 18.447096704675822 38.46810051404268 30.743927831196245 5 12.234424171951225 37.65650147406662 35.24259605281809 14.866478301164065 6 29.017046722242508 38.57909542209241 18.38877764847268 14.015080207192407 7 25.799326580973098 33.23388975152204 23.18758169551439 17.77920197199047 8 25.47580287414528 34.49386331849127 21.142662147732757 18.88767165963069 9 25.241699692850744 16.34157323454146 22.356412456757493 36.0603146158503 10-11 23.38969773860741 27.07038409438961 29.114196590135972 20.42572157686701 12-13 24.616675812391524 24.95067963854236 29.2301103460887 21.202534202977414 14-15 21.951088879355762 26.632138506992696 27.566414428792868 23.85035818485867 16-17 20.40201125660992 29.669560708888486 28.092117283106482 21.836310751395114 18-19 20.396851228168696 27.684035153827306 31.422508445076026 20.496605172927964 20-21 21.73710542353576 27.112619981701076 30.974599073264464 20.175675521498697 22-23 21.266254525390757 27.174279221541674 31.140896031727294 20.418570221340275 24-25 20.8203104062868 27.136715746844636 31.244142678711363 20.7988311681572 26-27 20.640246123683127 27.19828972311363 31.19865927628985 20.962804876913395 28-29 20.704939675733993 27.12013651303826 31.45475113516556 20.720172676062184 30-31 21.203892290493815 27.66474256861049 30.763449080160328 20.36791606073536 32-33 20.52546436892365 27.4003109256152 31.26544015553491 20.808784549926237 34-35 20.85248183112139 27.112814928318695 31.103397589510855 20.931305651049055 36-37 21.386698376852305 27.457610036501944 30.43025770362677 20.725433883018983 38-39 21.087188851133718 27.694635455285876 30.656117932092116 20.56205776148829 40-41 20.79737557771423 27.330022948522227 30.855969420563284 21.01663205320026 42-43 20.752096242965315 27.536919467665605 30.554764570304854 21.15621971906422 44-45 21.047209115038605 27.73268948870185 30.235931681975437 20.984169714284107 46-47 21.210272273310352 28.133559186214974 29.67071519801881 20.98545334245587 48-49 21.094649552674507 27.931268201139968 29.72179429670841 21.252287949477118 50-51 21.187449626635445 27.953343444485355 29.30354075852655 21.55566617035264 52-53 21.186441349415652 28.02398957636348 29.22643559724165 21.56313347697922 54-55 21.42193816992531 28.586832604112534 28.963718389857025 21.027510836105133 56-57 21.237049511897606 28.14305743549991 29.005987545727415 21.613905506875078 58-59 21.0282097272091 27.814044046561325 29.383315498632406 21.774430727597167 60-61 21.39274763118235 28.135438001733725 28.991441549210766 21.48037281787316 62-63 21.212855905598342 28.14258274101733 29.11356660557114 21.530994747813185 64-65 21.215064623123357 28.06718678284068 29.1761396566517 21.54160893738426 66-67 21.1409272336506 28.077609515980466 28.971917557221666 21.809545693147268 68-69 21.543603060006205 28.43847786338366 28.664564077160026 21.353354999450104 70-71 21.62458101966233 27.983035616908968 28.73991686967389 21.652466493754808 72-73 21.156934674236773 28.224039783573897 28.925767523257257 21.693258018932074 74-75 21.2344289317825 28.239807652623 28.778642408498733 21.747121007095764 76-77 21.328383562279853 28.519368153477075 28.52315539466559 21.629092889577475 78-79 21.400253572066948 28.34310810708024 28.81674529587528 21.43989302497753 80-81 21.27436962858778 28.23746926660079 28.71762415849592 21.77053694631551 82-83 21.095117021009408 28.256475419752764 28.99103730393533 21.657370255302506 84-85 21.014946431771754 28.278440378675263 28.740973518623896 21.965639670929086 86-87 21.207046904195085 28.423086973546614 28.632326903637278 21.73753921862102 88-89 20.92849047948858 28.309863456862967 28.73113711918935 22.030508944459104 90-91 20.841382989373166 28.114626124830778 28.79886488014779 22.245126005648263 92-93 20.978029389908983 28.376086822348107 28.50603923783328 22.139844549909625 94-95 20.864053772444965 28.078380329791674 28.791339337857526 22.266226559905835 96-97 20.698612241896928 28.24915612302428 28.69778318090041 22.354448454178375 98-99 20.67162530056878 28.154555006078123 28.660426461636412 22.513393231716673 100-101 20.60118559037076 28.277863433680146 28.401077204402625 22.719873771546464 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11567.0 1 7857.5 2 6982.0 3 8981.5 4 6242.0 5 4640.0 6 6971.5 7 13144.0 8 19811.5 9 22387.0 10 19455.5 11 15856.5 12 15570.5 13 16402.0 14 17305.5 15 18259.5 16 19443.5 17 20713.5 18 22207.0 19 24034.5 20 26740.5 21 31124.0 22 38309.5 23 50287.0 24 63388.0 25 77224.0 26 98089.0 27 125448.5 28 162401.0 29 197844.0 30 222493.5 31 251986.5 32 286616.5 33 317281.5 34 346543.0 35 374173.0 36 392303.5 37 406751.5 38 423206.5 39 432351.5 40 439947.5 41 440910.0 42 430651.0 43 424493.5 44 419765.5 45 413142.5 46 408106.0 47 403676.5 48 398419.0 49 396008.5 50 394616.0 51 376640.0 52 343307.5 53 316226.0 54 300616.5 55 290706.5 56 274330.0 57 248430.5 58 223002.0 59 201274.0 60 176898.0 61 148667.5 62 120561.5 63 95599.0 64 75922.5 65 60678.5 66 48065.0 67 36602.0 68 27102.0 69 19715.5 70 14328.5 71 10291.0 72 7409.0 73 5409.0 74 4067.5 75 2957.0 76 1976.0 77 1358.5 78 969.5 79 643.5 80 423.0 81 301.5 82 227.0 83 158.0 84 106.5 85 71.0 86 48.0 87 36.0 88 25.5 89 19.5 90 17.5 91 13.0 92 6.0 93 2.5 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048440914048514756 2 0.002194657047994535 3 1.980737407937306E-4 4 3.327638845334674E-4 5 0.0028126471192709748 6 0.001980737407937306 7 0.0 8 7.922949631749223E-6 9 8.715244594924146E-5 10-11 2.3768848895247672E-5 12-13 0.0 14-15 6.061056468288157E-4 16-17 0.0 18-19 4.7537697790495344E-5 20-21 4.278392801144581E-4 22-23 0.0 24-25 5.664908986700696E-4 26-27 0.007253460387866415 28-29 0.007415880855317274 30-31 0.0011924039195782582 32-33 0.006801852258856709 34-35 0.00801406355251434 36-37 0.004682463232363791 38-39 0.002634380752556617 40-41 0.0021391964005722905 42-43 0.0013627473366608666 44-45 7.170269416733047E-4 46-47 5.664908986700696E-4 48-49 0.0021510808250199145 50-51 0.001461784207057732 52-53 0.0019569685590420586 54-55 7.843720135431733E-4 56-57 2.376884889524767E-4 58-59 7.922949631749224E-4 60-61 0.006033326144577034 62-63 0.002717571723689984 64-65 0.0011963653943941328 66-67 0.005443066397011717 68-69 0.007400034956053775 70-71 0.0023412316161818955 72-73 5.664908986700696E-4 74-75 0.0034742134135220346 76-77 0.0018817005375404406 78-79 0.0023610389902612686 80-81 9.032162580194115E-4 82-83 1.980737407937306E-4 84-85 6.972195675939317E-4 86-87 0.0051023795628465 88-89 0.0024759217599216324 90-91 0.0012954022647909981 92-93 1.5053604300323525E-4 94-95 6.259130209081887E-4 96-97 4.278392801144581E-4 98-99 5.942212223811918E-4 100-101 1.5845899263498446E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2621562E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.463772201714384 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5156031056097 36.80462707095315 2 16.99548557928165 17.493035966193453 3 5.439621145652496 8.398302705104662 4 2.2104758555533603 4.550377035503515 5 1.0750370787201653 2.766273166382555 6 0.6419606817833187 1.9822630973852384 7 0.4042533810782582 1.4563082740909024 8 0.2775536617387359 1.1427166737179195 9 0.20193929028653945 0.9353301870494107 >10 1.082033797413215 10.259812533654584 >50 0.08788979678695974 3.1322605093989355 >100 0.06091340296734447 6.039560662399882 >500 0.004978301886448119 1.7279253597286708 >1k 0.00208503421955955 1.795030754395156 >5k 7.722137377351745E-5 0.2503685009059759 >10k+ 9.266564852822095E-5 1.2658075031359683 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51867 0.41093962854993704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44293 0.35093120803906835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29280 0.23198396521761727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.922949631749224E-6 0.0 2 0.0 0.0 0.0 7.922949631749224E-6 0.0 3 0.0 0.0 0.0 1.584589926349845E-5 0.0 4 0.0 0.0 0.0 3.961474815874612E-5 0.0 5 0.0 0.0 0.0 5.5460647422244566E-5 0.0 6 0.0 0.0 0.0 6.33835970539938E-5 0.0 7 0.0 0.0 0.0 7.130654668574302E-5 0.0 8 0.0 0.0 0.0 7.922949631749224E-5 0.0 9 1.584589926349845E-5 0.0 0.0 1.1884424447623836E-4 0.0 10-11 1.584589926349845E-5 0.0 0.0 1.2676719410798756E-4 0.0 12-13 1.584589926349845E-5 0.0 0.0 1.5449751781910988E-4 0.0 14-15 3.16917985269969E-5 7.922949631749224E-6 0.0 2.297655393207275E-4 0.0 16-17 3.16917985269969E-5 1.980737407937306E-5 0.0 2.693802874794736E-4 0.0 18-19 3.961474815874612E-5 3.961474815874612E-5 0.0 2.9314913637472134E-4 0.0 20-21 4.7537697790495344E-5 3.961474815874612E-5 0.0 3.644556830604643E-4 0.0 22-23 5.1499172606369955E-5 3.961474815874612E-5 0.0 5.546064742224456E-4 0.0 24-25 5.5460647422244566E-5 4.357622297462073E-5 0.0 8.675629846765401E-4 0.0 26-27 5.5460647422244566E-5 4.7537697790495344E-5 0.0 0.0013548243870291172 0.0 28-29 5.5460647422244566E-5 4.7537697790495344E-5 0.0 0.002666072551083614 0.0 30-31 5.5460647422244566E-5 4.7537697790495344E-5 0.0 0.006061056468288157 0.0 32-33 5.5460647422244566E-5 4.7537697790495344E-5 0.0 0.01306494394275447 7.922949631749224E-6 34-35 5.5460647422244566E-5 4.7537697790495344E-5 0.0 0.024046152132358896 7.922949631749224E-6 36-37 5.5460647422244566E-5 5.942212223811918E-5 0.0 0.04165490768892154 1.1884424447623838E-5 38-39 5.5460647422244566E-5 7.526802150161762E-5 0.0 0.06586744176354717 1.584589926349845E-5 40-41 5.942212223811918E-5 7.922949631749224E-5 0.0 0.09267078036775481 1.584589926349845E-5 42-43 6.33835970539938E-5 9.507539558099069E-5 0.0 0.12087251958196615 1.584589926349845E-5 44-45 6.33835970539938E-5 1.0299834521273991E-4 0.0 0.15489762677551322 1.584589926349845E-5 46-47 8.715244594924146E-5 1.346901437397368E-4 0.0 0.19613261813395205 1.584589926349845E-5 48-49 8.715244594924146E-5 1.5053604300323525E-4 0.0 0.24066355653919858 1.584589926349845E-5 50-51 8.715244594924146E-5 1.8222784153023217E-4 0.0 0.287123733179776 1.584589926349845E-5 52-53 8.715244594924146E-5 1.8618931634610677E-4 0.0 0.3332867992091629 1.584589926349845E-5 54-55 8.715244594924146E-5 1.9411226597785598E-4 0.0 0.3866280576049145 1.584589926349845E-5 56-57 8.715244594924146E-5 1.980737407937306E-4 0.0 0.4486845605955903 1.584589926349845E-5 58-59 9.507539558099069E-5 1.980737407937306E-4 0.0 0.5177964502333388 1.584589926349845E-5 60-61 9.507539558099069E-5 1.980737407937306E-4 0.0 0.5919790276354068 1.584589926349845E-5 62-63 9.507539558099069E-5 2.0599669042547982E-4 0.0 0.6680036908268565 1.584589926349845E-5 64-65 9.507539558099069E-5 2.0599669042547982E-4 0.0 0.7520146872471094 1.584589926349845E-5 66-67 9.507539558099069E-5 2.0599669042547982E-4 0.0 0.8510040199461841 1.584589926349845E-5 68-69 9.507539558099069E-5 2.4164996376835132E-4 0.0 0.9637198628822645 1.584589926349845E-5 70-71 9.507539558099069E-5 2.456114385842259E-4 0.0 1.08828051551781 1.584589926349845E-5 72-73 1.0299834521273991E-4 2.4957291340010055E-4 0.0 1.2260645710887448 1.980737407937306E-5 74-75 1.9015079116198137E-4 2.6541881266359897E-4 0.0 1.3699255290272314 2.3768848895247672E-5 76-77 1.9015079116198137E-4 2.693802874794736E-4 0.0 1.5248033484286652 2.3768848895247672E-5 78-79 1.980737407937306E-4 2.693802874794736E-4 0.0 1.7018456194249174 2.3768848895247672E-5 80-81 1.980737407937306E-4 2.7730323711122287E-4 0.0 1.8989646447880222 2.3768848895247672E-5 82-83 1.980737407937306E-4 2.852261867429721E-4 0.0 2.1106618974735456 2.3768848895247672E-5 84-85 1.980737407937306E-4 2.931491363747213E-4 0.0 2.32637212414755 2.3768848895247672E-5 86-87 1.980737407937306E-4 3.050335608223451E-4 0.0 2.5595524547595616 2.3768848895247672E-5 88-89 1.980737407937306E-4 3.0899503563821976E-4 0.0 2.82637362950798 2.3768848895247672E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 26130 0.0 58.4123 1 GTATCAA 48665 0.0 41.938667 1 TATAACG 1410 0.0 35.033855 2 TACCTGG 11060 0.0 32.380978 2 ATCAACG 62345 0.0 32.378178 3 TCAACGC 63135 0.0 31.98808 4 TATCAAC 63890 0.0 31.662737 2 GTACCTG 11405 0.0 31.582178 1 CAACGCA 65360 0.0 30.935469 5 GTGGTAT 10765 0.0 30.016706 1 AACGCAG 69970 0.0 29.100346 6 TGGTATC 10605 0.0 28.977926 2 ATAACGC 1880 0.0 28.801302 3 CGCAGAG 79220 0.0 25.43229 8 ACGCAGA 80215 0.0 25.28251 7 GTACATG 48680 0.0 23.691217 1 TACATGG 47780 0.0 23.033165 2 CCGAGGG 4010 0.0 22.978544 4 GCAGAGT 88675 0.0 22.3243 9 ACCTGGG 15945 0.0 22.07287 3 >>END_MODULE