FastQCFastQC Report
Wed 25 May 2016
SRR2049427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390862
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70250.043.4721
GTATCAA118950.033.9247631
ATCAACG122600.031.283973
TCAACGC122700.031.2197884
CAACGCA136400.028.0800845
TATCAAC145400.026.7367552
AACGCAG148400.025.8562646
GTACATG267900.023.670271
TACATGG263800.023.1035882
ACGCAGA166500.022.620777
CGCAGAG163100.022.2529688
ACATGGG264950.022.018563
CATGGGT67350.018.8885294
CATGGGG147700.018.0294864
GAGTACA204800.017.7730221
GCAGAGT202350.017.1862559
AGTACAT214950.015.590372
AGAGTAC179700.015.5034835
CAGAGTA195300.015.41552610-11
TCTGTCG12950.015.3872138
Produced by FastQC (version 0.11.5)