##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049427_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7390862 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.097728384050466 31.0 31.0 34.0 28.0 34.0 2 31.117169688731842 31.0 31.0 34.0 28.0 34.0 3 31.311640103684795 31.0 31.0 34.0 28.0 34.0 4 34.908566957413086 37.0 35.0 37.0 32.0 37.0 5 35.05103125995317 37.0 35.0 37.0 32.0 37.0 6 35.063971428501844 37.0 35.0 37.0 32.0 37.0 7 35.04770945527058 37.0 35.0 37.0 32.0 37.0 8 35.049344582539895 37.0 35.0 37.0 32.0 37.0 9 36.799386999784325 39.0 37.0 39.0 32.0 39.0 10-11 36.74586834390901 39.0 37.0 39.0 32.0 39.0 12-13 36.76401243860323 39.0 37.0 39.0 32.0 39.0 14-15 37.869654378609695 40.0 37.5 41.0 32.0 41.0 16-17 37.82572262883545 40.0 37.0 41.0 32.0 41.0 18-19 37.85187756177831 40.0 37.5 41.0 32.0 41.0 20-21 37.94573921147493 40.0 38.0 41.0 32.0 41.0 22-23 37.76923361578122 40.0 37.0 41.0 32.0 41.0 24-25 37.77588324609498 40.0 37.0 41.0 31.5 41.0 26-27 37.77190285788045 40.0 37.0 41.0 32.0 41.0 28-29 37.41832400604963 40.0 37.0 41.0 31.0 41.0 30-31 37.4559512138097 40.0 37.0 41.0 31.0 41.0 32-33 37.436940643730054 40.0 37.0 41.0 30.5 41.0 34-35 37.52854484361905 40.0 37.0 41.0 31.0 41.0 36-37 37.54342747841862 40.0 37.0 41.0 31.0 41.0 38-39 37.326063116859714 40.0 36.5 41.0 30.0 41.0 40-41 37.46820939154323 40.0 37.0 41.0 31.0 41.0 42-43 37.10491604362252 39.5 36.0 41.0 30.0 41.0 44-45 37.05099777265494 39.0 36.0 41.0 30.0 41.0 46-47 36.98577851947445 39.0 36.0 41.0 30.0 41.0 48-49 36.84544306198654 39.0 35.0 40.5 30.0 41.0 50-51 36.74569941909347 39.0 35.0 40.0 30.0 41.0 52-53 36.60892883671755 39.0 35.0 40.0 30.0 41.0 54-55 36.42439251334959 39.0 35.0 40.0 29.5 41.0 56-57 36.20273142699729 38.0 34.5 40.0 29.0 41.0 58-59 35.94487287139172 38.0 34.0 40.0 28.5 41.0 60-61 35.69534676740007 38.0 34.0 40.0 28.0 41.0 62-63 35.429244856689245 38.0 34.0 40.0 28.0 41.0 64-65 35.12369504396104 37.0 34.0 40.0 28.0 41.0 66-67 34.84275595999492 37.0 33.0 40.0 28.0 41.0 68-69 34.52587756340194 36.5 33.0 39.0 27.0 41.0 70-71 34.1727908733785 36.0 33.0 39.0 26.0 40.5 72-73 33.794751613546566 35.5 32.0 39.0 26.0 40.0 74-75 33.404411691085556 35.0 32.0 38.0 26.0 40.0 76-77 33.0382632634732 35.0 32.0 37.5 26.0 39.5 78-79 32.81924334401048 35.0 32.0 37.0 26.0 39.0 80-81 32.683922863124764 35.0 32.0 37.0 26.0 39.0 82-83 32.365415292559916 35.0 32.0 36.0 26.0 38.5 84-85 32.02647952295686 35.0 32.0 36.0 25.0 37.0 86-87 31.58592752780393 34.5 31.5 35.5 24.5 37.0 88-89 31.143083377825214 34.0 31.0 35.0 23.0 36.5 90-91 30.91097804288593 34.0 31.0 35.0 22.5 36.0 92-93 30.66481927277224 34.0 31.0 35.0 20.0 36.0 94-95 30.384556767532665 34.0 31.0 35.0 19.5 36.0 96-97 30.10306869482883 34.0 31.0 35.0 17.5 35.0 98-99 29.77695402782517 34.0 31.0 35.0 4.5 35.0 100-101 29.188925053667624 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29243340774452875 1101 2 0.35876224510951715 1101 3 0.2850233887706537 1101 4 0.2652557242605198 1101 5 0.2642416643286154 1101 6 0.09729229981886789 1101 7 0.18240548743572305 1101 8 0.32438131111368307 1101 9 0.32250990310737393 1101 10-11 0.340135812421849 1101 12-13 0.32542853457763954 1101 14-15 0.5347812157959027 1101 16-17 0.4685134610815922 1101 18-19 0.5190429411336837 1101 20-21 0.528196873544367 1101 22-23 0.4770696423696208 1101 24-25 0.11941226215749623 1101 26-27 0.3761918938083255 1101 28-29 0.8372557872865798 1101 30-31 0.38490030984306145 1101 32-33 0.7463594245272773 1101 34-35 0.5994347922112411 1101 36-37 0.5947761891610028 1101 38-39 0.5807962186549815 1101 40-41 0.451409539047809 1101 42-43 0.5659563375958925 1101 44-45 0.33883100701572033 1101 46-47 0.22047506513930415 1101 48-49 0.14019255196730995 1101 50-51 0.12406214645589841 1101 52-53 0.077103043410375 1101 54-55 0.032278058589824354 1101 56-57 -0.08597868323249003 1101 58-59 -0.05278388400792977 1101 60-61 -0.03387269971108253 1101 62-63 -0.14479006126310878 1101 64-65 -0.09856476985604701 1101 66-67 -0.10750138472421611 1101 68-69 -0.2958200894873997 1101 70-71 -0.3074447924612471 1101 72-73 -0.33843384525300024 1101 74-75 -0.35796031268098716 1101 76-77 -0.4320822862348024 1101 78-79 -0.38806224520573096 1101 80-81 -0.47239623010482035 1101 82-83 -0.5451317978118908 1101 84-85 -0.5013156878203624 1101 86-87 -0.5689431466270953 1101 88-89 -0.6120424271968616 1101 90-91 -0.6417188437475474 1101 92-93 -0.734144869271411 1101 94-95 -0.7909003355185682 1101 96-97 -0.9049181716177124 1101 98-99 -0.9319142396644793 1101 100-101 -1.0707912363007068 1102 1 0.12733121353960541 1102 2 0.06944020208688073 1102 3 0.011747093425164223 1102 4 0.06284162032282836 1102 5 0.13777173590906955 1102 6 -0.1297953595085204 1102 7 -0.11327447380904232 1102 8 0.08348351555500244 1102 9 0.16038912796693694 1102 10-11 0.18209649098005798 1102 12-13 0.22716858894213487 1102 14-15 0.345957050001104 1102 16-17 0.41718819803479334 1102 18-19 0.39992383030138257 1102 20-21 0.29246790957255797 1102 22-23 -0.25905435159251056 1102 24-25 0.2668311978580533 1102 26-27 0.4503418329896647 1102 28-29 0.5160512316720158 1102 30-31 0.4072343443693711 1102 32-33 0.6775414633129131 1102 34-35 0.018064418425311146 1102 36-37 0.22129822837892021 1102 38-39 0.3820817501567575 1102 40-41 0.18700501366144096 1102 42-43 0.15608755752707282 1102 44-45 -0.05003756453283614 1102 46-47 -0.15338825193266103 1102 48-49 -0.2549704922030074 1102 50-51 -0.21423786510320753 1102 52-53 -0.1724770910113591 1102 54-55 -0.13944555618791554 1102 56-57 -0.31102048980553576 1102 58-59 -0.3463270019030986 1102 60-61 -0.3773108221135004 1102 62-63 -0.4069347639740286 1102 64-65 -0.4651012840130875 1102 66-67 -0.4684180223389518 1102 68-69 -0.4543578146221563 1102 70-71 -0.6145890021603151 1102 72-73 -0.6622502036392603 1102 74-75 -0.6533375140799009 1102 76-77 -0.5952613526430071 1102 78-79 -0.6027356948399145 1102 80-81 -0.6561147478367673 1102 82-83 -0.6896635925597394 1102 84-85 -0.7082442187063833 1102 86-87 -0.7333320268263535 1102 88-89 -0.796717539011734 1102 90-91 -0.9217923666459455 1102 92-93 -0.9234481067745222 1102 94-95 -0.9647133038701945 1102 96-97 -0.9964083383435884 1102 98-99 -1.0831048355511683 1102 100-101 -1.2788929673704779 1103 1 0.20739557044424828 1103 2 0.10773300652371631 1103 3 0.10743599511991064 1103 4 0.1702223486841561 1103 5 -0.04223438988499595 1103 6 -0.13411760127429062 1103 7 -0.031898980262404564 1103 8 0.12371262062598731 1103 9 0.2715040116037386 1103 10-11 0.26128904466105496 1103 12-13 0.2519442653920123 1103 14-15 0.3398607974591954 1103 16-17 0.43206672327738715 1103 18-19 0.47512742776115857 1103 20-21 0.44571293642241727 1103 22-23 -0.007353403705010919 1103 24-25 0.35501298588039276 1103 26-27 0.5903609184977157 1103 28-29 0.5172217031122202 1103 30-31 0.5717690391326045 1103 32-33 0.5777426371322036 1103 34-35 0.5283746611963522 1103 36-37 0.4018476794904231 1103 38-39 0.5977675987485327 1103 40-41 0.40121635141118617 1103 42-43 0.0572270059027602 1103 44-45 0.18936214091789338 1103 46-47 0.11692082923212155 1103 48-49 0.06563779585146534 1103 50-51 0.04050002556346044 1103 52-53 -0.04650958585836662 1103 54-55 0.005208912742396876 1103 56-57 -0.0103879079810838 1103 58-59 -0.0828402743584391 1103 60-61 -0.13707653085609195 1103 62-63 -0.10222424381041151 1103 64-65 -0.2112982360962974 1103 66-67 -0.2984530281645519 1103 68-69 -0.34743888771252784 1103 70-71 -0.3781353529708156 1103 72-73 -0.4674420511842783 1103 74-75 -0.3522897419651656 1103 76-77 -0.39763987283311053 1103 78-79 -0.36460641462320353 1103 80-81 -0.44754235400660036 1103 82-83 -0.4717033307182632 1103 84-85 -0.42622778807182726 1103 86-87 -0.37329307088652186 1103 88-89 -0.3587712700795542 1103 90-91 -0.41681536884406967 1103 92-93 -0.44909162607337194 1103 94-95 -0.46930005124859875 1103 96-97 -0.45438411474881235 1103 98-99 -0.6228382874889782 1103 100-101 -0.5240628026944449 1104 1 0.2623260253264874 1104 2 0.3776952629854904 1104 3 0.2175786867476539 1104 4 0.25605538895307234 1104 5 0.11835893271411635 1104 6 -0.056722700832139594 1104 7 0.08713854089661766 1104 8 0.11153253612189218 1104 9 0.32052796323135624 1104 10-11 0.1890875616591856 1104 12-13 0.27462432061786757 1104 14-15 0.4993377815504516 1104 16-17 0.5677393594321813 1104 18-19 0.4833501271603282 1104 20-21 0.45900106675490804 1104 22-23 0.6003841570219421 1104 24-25 0.7339914834702697 1104 26-27 0.6726705431168867 1104 28-29 0.5350074273829932 1104 30-31 0.7111069549893259 1104 32-33 0.6883875858919097 1104 34-35 0.5841535079588098 1104 36-37 0.40843033228811265 1104 38-39 0.5239456161753466 1104 40-41 0.406687700542129 1104 42-43 0.46317473562098144 1104 44-45 0.2536110289372786 1104 46-47 0.12581658957436304 1104 48-49 0.08418462732019094 1104 50-51 0.04404523065158372 1104 52-53 0.10387972159458769 1104 54-55 0.05986181672027868 1104 56-57 0.04116205304331544 1104 58-59 0.05125226788261017 1104 60-61 0.0378148942764156 1104 62-63 0.03791281556483739 1104 64-65 0.043952891623490586 1104 66-67 -0.08092707630880369 1104 68-69 -0.13464809813775247 1104 70-71 -0.15954594959355717 1104 72-73 -0.1509585822176902 1104 74-75 -0.007046075964503018 1104 76-77 -0.14163381511538375 1104 78-79 -0.1775900343811756 1104 80-81 -0.14350145823474492 1104 82-83 -0.09751262853816911 1104 84-85 -0.18974197853876973 1104 86-87 -0.13976186688762127 1104 88-89 -0.15411031035591094 1104 90-91 -0.11092634791165779 1104 92-93 -0.1708552640616965 1104 94-95 -0.18911677118586212 1104 96-97 -0.24048485624974703 1104 98-99 -0.31663596404943206 1104 100-101 -0.3056634963009266 1105 1 0.24743566888029633 1105 2 0.14008741849263373 1105 3 0.0842303348675344 1105 4 0.10843083532452624 1105 5 0.007588777873486663 1105 6 0.05767997193458996 1105 7 0.0313661287393856 1105 8 -0.13089530533117255 1105 9 -0.15170375854947338 1105 10-11 0.14020489352136423 1105 12-13 0.16657598621451086 1105 14-15 0.3277167366790721 1105 16-17 0.033923867931008544 1105 18-19 0.1454541894796222 1105 20-21 0.07439482804942088 1105 22-23 0.3955779805233348 1105 24-25 0.06330212883140973 1105 26-27 -0.07476367152887065 1105 28-29 0.45489974749558826 1105 30-31 0.36716871569856835 1105 32-33 0.37982853497929625 1105 34-35 -0.36185701203106646 1105 36-37 -0.4008703190377716 1105 38-39 -0.25240201224269754 1105 40-41 -0.1417983775313516 1105 42-43 0.16531159740780765 1105 44-45 -0.0398467357101282 1105 46-47 -0.30163247865473863 1105 48-49 -0.29535517164264036 1105 50-51 -0.40324545052438765 1105 52-53 -0.24095195512105505 1105 54-55 -0.33272303928625746 1105 56-57 -0.49963470950329025 1105 58-59 -0.16926184126023713 1105 60-61 -0.16954853539604642 1105 62-63 -0.25098850699806974 1105 64-65 -0.4279792133947211 1105 66-67 -0.44894764893032146 1105 68-69 -0.38298322034115273 1105 70-71 -0.24895747220031694 1105 72-73 -0.23776762512795813 1105 74-75 -0.11593327525097408 1105 76-77 -0.21455764732193927 1105 78-79 -0.23312546433303538 1105 80-81 -0.22993099930769034 1105 82-83 -0.29723035107540596 1105 84-85 -0.3512146881485698 1105 86-87 -0.20636458253529355 1105 88-89 -0.35610851406821453 1105 90-91 -0.2401888426585792 1105 92-93 -0.21092176265849005 1105 94-95 -0.15125331736553704 1105 96-97 -0.26211962086719964 1105 98-99 -0.267565564076758 1105 100-101 -0.39694159267549267 1106 1 0.40928354579929405 1106 2 0.4010409039194265 1106 3 0.31550501166735145 1106 4 0.33125785699445487 1106 5 0.3668817467103267 1106 6 0.18313447487256695 1106 7 0.14311745894782746 1106 8 0.25847015508072246 1106 9 0.32826843551426776 1106 10-11 0.07354527781055253 1106 12-13 0.23310878913790845 1106 14-15 0.47308097089511136 1106 16-17 0.4537105344745669 1106 18-19 0.4721001198511843 1106 20-21 0.4190749244513228 1106 22-23 0.5772507079619373 1106 24-25 0.5269045506561412 1106 26-27 0.6065110863322118 1106 28-29 0.611015095637633 1106 30-31 0.6438816095419213 1106 32-33 0.6736531176944709 1106 34-35 0.5948858546946809 1106 36-37 0.34072962528001227 1106 38-39 0.5200405468541902 1106 40-41 0.3924493323300098 1106 42-43 -0.4628843561843752 1106 44-45 0.18699802557119227 1106 46-47 0.18492602535994962 1106 48-49 0.12272194036937378 1106 50-51 0.2667480939765525 1106 52-53 0.18674635926358718 1106 54-55 0.10668818776444056 1106 56-57 0.03118214428162247 1106 58-59 0.024689675147293144 1106 60-61 0.11353203655792754 1106 62-63 0.09220616239137769 1106 64-65 0.19693825181467872 1106 66-67 0.16965757582796215 1106 68-69 0.10995975108276923 1106 70-71 0.1919104257237194 1106 72-73 0.10719207767013472 1106 74-75 0.18007698012525708 1106 76-77 0.18415630614323675 1106 78-79 0.20328563046415127 1106 80-81 0.1513449868375929 1106 82-83 0.14878173573576703 1106 84-85 0.20989620176146673 1106 86-87 0.24466920822261784 1106 88-89 0.2072623500797306 1106 90-91 0.2675880786176634 1106 92-93 0.2838792290587264 1106 94-95 0.424138368590274 1106 96-97 0.39000817267905674 1106 98-99 0.355717156528236 1106 100-101 0.3873218829143106 1107 1 0.22660366249903774 1107 2 -0.013880780934812265 1107 3 0.10363417276524345 1107 4 0.0947230789624598 1107 5 0.03831017786303903 1107 6 -0.11872833543863237 1107 7 0.024081621335064085 1107 8 -0.440976929098845 1107 9 -0.15932732680348494 1107 10-11 -0.43868502649743846 1107 12-13 -0.23828416805979202 1107 14-15 -0.007120407771729731 1107 16-17 0.16468827389910956 1107 18-19 0.04549606676766871 1107 20-21 -0.3734554901454601 1107 22-23 0.07301437309589431 1107 24-25 0.25416981803611804 1107 26-27 -0.12070532408158385 1107 28-29 -0.35985784936695353 1107 30-31 -0.08370184495404942 1107 32-33 -0.4981334894092555 1107 34-35 -0.388405016381725 1107 36-37 -0.9561982738599326 1107 38-39 -0.46295995729191475 1107 40-41 -0.5113895414791259 1107 42-43 -0.41365364117463344 1107 44-45 -0.23731942637671466 1107 46-47 -0.27152799274701067 1107 48-49 -0.5316502138589527 1107 50-51 -0.39590259658016436 1107 52-53 -0.34005564338070116 1107 54-55 -0.14714075123468007 1107 56-57 -0.3194479139099755 1107 58-59 -0.19670138148116934 1107 60-61 -0.1417138331296428 1107 62-63 -0.10321863786236918 1107 64-65 -0.10636274766876141 1107 66-67 -0.15658766051056006 1107 68-69 -0.0772457541042968 1107 70-71 0.03452284228453095 1107 72-73 -0.0011912713866095714 1107 74-75 0.22507297298151485 1107 76-77 0.08734363692507685 1107 78-79 0.04389692104406606 1107 80-81 0.07005812674540124 1107 82-83 0.07916254351509622 1107 84-85 0.06407277936928324 1107 86-87 0.16909287392056171 1107 88-89 -0.034717485520708635 1107 90-91 0.0812851525112741 1107 92-93 0.1506362591213133 1107 94-95 0.23152159055382526 1107 96-97 0.19326742400467367 1107 98-99 0.21257084414283867 1107 100-101 0.21124514214075063 1108 1 0.3708259461540564 1108 2 0.3253298859567586 1108 3 0.2952152585960448 1108 4 0.25337932411374453 1108 5 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0.3586488967347563 2214 52-53 0.3158239567996972 2214 54-55 0.3148179526722501 2214 56-57 0.28668309366068456 2214 58-59 0.23755123517609888 2214 60-61 0.21784446226481435 2214 62-63 0.3461299487514111 2214 64-65 0.3333948007250598 2214 66-67 0.39424347977033847 2214 68-69 0.5338506288250144 2214 70-71 0.44470872133992145 2214 72-73 0.5470945334733486 2214 74-75 0.5004141115369904 2214 76-77 0.4914780254374733 2214 78-79 0.5365345619856186 2214 80-81 0.5362053405497988 2214 82-83 0.5251926451432496 2214 84-85 0.3857755924374189 2214 86-87 0.3582545244847779 2214 88-89 0.4766044564293992 2214 90-91 0.37844304545488683 2214 92-93 0.238264514219793 2214 94-95 0.20586095119964298 2214 96-97 0.292207316307195 2214 98-99 0.40641923781414135 2214 100-101 0.39647740107760043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 98.0 11 1809.0 12 7539.0 13 15338.0 14 21595.0 15 25391.0 16 27712.0 17 28904.0 18 29987.0 19 31320.0 20 32686.0 21 35503.0 22 40382.0 23 49906.0 24 61428.0 25 72801.0 26 84141.0 27 97757.0 28 116526.0 29 139374.0 30 169609.0 31 209771.0 32 266161.0 33 344406.0 34 461902.0 35 653603.0 36 972996.0 37 1416652.0 38 1544244.0 39 431040.0 40 281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.470962534252955 21.283895388616845 13.59967005736626 23.645472019763943 2 14.949046894566397 21.105960011150962 40.218624410346784 23.726368683935856 3 17.81752626800986 26.632705491623547 30.227779067775902 25.32198917259069 4 12.150788226325888 17.64042784650601 39.9887625491977 30.220021377970397 5 12.315589832927824 38.95571393772906 36.00903732527452 12.719658904068599 6 29.04917347846406 39.16225378014503 18.664568397643897 13.124004343747014 7 25.986278615759566 33.31835917097985 23.48458319779912 17.210779015461455 8 24.3880516640914 36.651115843708816 21.237251076335237 17.723581415864544 9 24.44788160994667 17.654784087818225 22.8072575614258 35.09007674080931 10-11 23.02906561589765 27.671533881245917 29.85721055734664 19.442189945509792 12-13 23.48229436739434 25.240439115358072 29.725744709886158 21.551521807361436 14-15 20.946386996222845 27.365037207271985 27.122723782857943 24.56585201364722 16-17 20.4428499841602 30.73058088427635 27.756805396980873 21.069763734582583 18-19 20.44694541115143 29.78282481591522 29.53936079480296 20.23086897813039 20-21 21.76159055362193 28.852909361778327 28.926664370532933 20.458835714066808 22-23 21.430166729916504 28.70458621444869 28.955621210295448 20.90962584533936 24-25 21.01873951934788 29.021882048501844 28.915436960503786 21.043941471646484 26-27 20.803622089828224 29.071182570465208 28.94622739906318 21.178967940643386 28-29 20.86790781653259 28.844096649701793 29.26170336615626 21.026292167609355 30-31 21.102228392092552 29.265142735755685 28.822129809471424 20.810499062680336 32-33 20.62596184784112 29.074432782074606 29.224648146689862 21.074957223394414 34-35 21.037971605776914 28.908674131865975 28.990513542470676 21.062840719886434 36-37 21.333378073507927 28.96154852599616 28.664707389786955 21.040366010708958 38-39 20.96681043220487 29.076953180215664 29.017391591168817 20.938844796410645 40-41 20.823187117774765 28.86418585719938 29.198997439337855 21.113629585688003 42-43 20.855918658730705 28.84712546877799 29.02783062345511 21.269125249036193 44-45 20.824680427149893 28.970829754784074 29.02494946278798 21.179540355278057 46-47 21.03874629495193 28.949183756176204 28.873327104409796 21.138742844462065 48-49 20.90602976084638 28.85928753779781 28.983543658722667 21.251139042633145 50-51 20.959169285147865 28.860257003591443 28.85707016988327 21.323503541377416 52-53 20.958536252024675 28.809923316017304 28.88236511968087 21.34917531227715 54-55 21.005573567141834 29.15825597868406 28.76867663249889 21.067493821675214 56-57 20.993356325982507 28.85397502742826 28.798604311554726 21.354064335034508 58-59 21.00291953052861 28.51893959923085 29.040712054476252 21.437428815764285 60-61 21.137305615690323 28.809347850122492 28.770400278993442 21.282946255193743 62-63 20.912478661554168 28.806034161675377 28.87333344293908 21.408153733831377 64-65 21.032553010428295 28.65309873418304 28.876945464771055 21.437402790617615 66-67 20.87871008617315 28.674273727250842 28.84893805576782 21.59807813080819 68-69 21.21807678289895 28.850441803651407 28.75121078820494 21.180270625244706 70-71 21.216523281351645 28.598280458645387 28.781812489243396 21.40338377075957 72-73 20.962136049172074 28.590703315821465 28.971887434448885 21.47527320055758 74-75 21.017041907271082 28.695707833531458 28.88818685465787 21.399063404539586 76-77 20.990228491780474 28.677213253175594 28.75943082971001 21.573127425333922 78-79 21.10638114749606 28.644442077733462 28.864767232245935 21.38440954252454 80-81 20.96494859090124 28.578723377763303 28.87149007652577 21.58483795480969 82-83 20.914446641552306 28.520872870669894 29.115161515406935 21.449518972370864 84-85 20.8900714501838 28.392959972020606 28.951891400647334 21.76507717714826 86-87 21.08015677071499 28.508130354234446 28.840179532353154 21.571533342697414 88-89 20.96412884184331 28.482736932814834 28.879695926606352 21.6734382987355 90-91 20.833671942158123 28.32604048196117 29.006797359463725 21.83349021641698 92-93 20.98342355661468 28.486930727170378 28.807650498627424 21.721995217587516 94-95 20.932621842818694 28.19505087481154 29.033742030647396 21.83858525172237 96-97 20.973704568768987 28.291002164306683 28.828944772373482 21.906348494550848 98-99 20.91707847109392 28.210133005972548 28.842604437625337 22.030184085308193 100-101 20.989896328025026 28.24726126064566 28.779661764447656 21.983180646881653 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4437.0 1 2689.5 2 722.0 3 429.0 4 294.5 5 185.0 6 127.0 7 147.0 8 208.5 9 205.0 10 155.0 11 135.5 12 141.0 13 183.0 14 251.5 15 366.5 16 650.0 17 1145.5 18 1929.5 19 3098.5 20 5098.0 21 8241.0 22 13445.5 23 21679.5 24 31619.5 25 43425.5 26 60341.5 27 81490.0 28 107319.0 29 133481.0 30 154560.5 31 176375.0 32 199135.0 33 217480.0 34 234239.5 35 250274.0 36 262429.0 37 272173.5 38 280338.0 39 285620.0 40 288298.0 41 283437.0 42 274637.5 43 269500.5 44 263904.5 45 258846.0 46 253270.0 47 246015.0 48 236581.0 49 230012.5 50 223924.5 51 207995.0 52 187963.0 53 169456.5 54 155971.0 55 148351.5 56 135291.0 57 118172.5 58 104066.0 59 91453.5 60 78627.0 61 64370.0 62 52145.0 63 42639.5 64 34497.5 65 28090.0 66 22823.0 67 17902.5 68 13411.0 69 9749.0 70 7123.0 71 5071.0 72 3705.0 73 2798.5 74 2199.0 75 1633.5 76 1036.5 77 681.5 78 513.0 79 312.0 80 150.5 81 72.0 82 38.5 83 21.5 84 12.0 85 8.0 86 8.0 87 7.5 88 2.5 89 1.0 90 1.0 91 1.0 92 1.5 93 2.0 94 1.0 95 1.0 96 2.0 97 3.5 98 3.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43306179982794973 2 0.06701518713243462 3 0.06733991244864265 4 0.0896377174949282 5 0.07429444630409822 6 1.0824177206934725E-4 7 0.004789698414068616 8 5.682693033640731E-4 9 0.014233793027119166 10-11 0.0011433037174824804 12-13 0.009274966844192193 14-15 0.00865934176554778 16-17 0.01689924666432684 18-19 0.00883523464516047 20-21 0.002401614317788642 22-23 0.01158863472217449 24-25 0.03444117885031543 26-27 0.002861641849083368 28-29 0.004004945566565849 30-31 0.0014477337014275197 32-33 0.0018942310112135769 34-35 0.0022798423242106265 36-37 5.412088603467363E-4 38-39 0.11619077720568996 40-41 0.007928709804079686 42-43 0.0050061819582073105 44-45 0.010323559011113995 46-47 0.008212844455761724 48-49 0.011960715813662872 50-51 0.014639699672379216 52-53 0.010715935434865377 54-55 0.009626752603417572 56-57 0.009119369296842506 58-59 0.015512398959688329 60-61 0.019497049193991176 62-63 0.009992068584151617 64-65 0.0163377424717171 66-67 0.013164905527934359 68-69 0.007191312731857258 70-71 7.30631961468094E-4 72-73 0.009910887255099608 74-75 0.016581286458873135 76-77 0.026126857733238692 78-79 0.017981664385020312 80-81 0.02764900765296389 82-83 0.05931649109400229 84-85 0.034738843723506135 86-87 0.3496006284517286 88-89 0.6124725370328927 90-91 0.603813195267345 92-93 0.611525421527286 94-95 0.6033666979575589 96-97 0.5975960584841119 98-99 0.6066748371164283 100-101 0.5997270683717271 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7390862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.018601263499164 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36883943868602 38.55244880277075 2 16.288663839953088 17.597816741712062 3 5.697250625451872 9.232725295035179 4 2.5397300818195183 5.487706664268907 5 1.2973649707724912 3.504092052469523 6 0.7339124109658167 2.378695313417747 7 0.4609718428944095 1.7430737922509443 8 0.3087578712117683 1.3342934665564272 9 0.20929879751117159 1.0175425458917249 >10 0.9623341512044428 9.140518421376864 >50 0.07289434473252925 2.7398033193488556 >100 0.05655952518375039 5.807065791380888 >500 0.002843433129211695 1.0109522731712757 >1k 5.535070723001671E-4 0.3824440587874123 >5k 2.515941144662199E-5 0.07082146156153382 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3530221508668407E-5 2 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 3 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 4 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 5 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 6 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 7 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 8 0.0 1.3530221508668407E-5 0.0 0.0 1.3530221508668407E-5 9 0.0 1.3530221508668407E-5 0.0 4.059066452600522E-5 1.3530221508668407E-5 10-11 0.0 4.059066452600522E-5 0.0 6.765110754334204E-5 1.3530221508668407E-5 12-13 0.0 6.765110754334204E-5 0.0 8.118132905201045E-5 1.3530221508668407E-5 14-15 0.0 6.765110754334204E-5 0.0 9.471155056067884E-5 2.7060443017336813E-5 16-17 0.0 6.765110754334204E-5 0.0 1.0824177206934725E-4 2.7060443017336813E-5 18-19 0.0 6.765110754334204E-5 0.0 1.1500688282368146E-4 2.7060443017336813E-5 20-21 0.0 6.765110754334204E-5 0.0 1.5559754734968667E-4 2.7060443017336813E-5 22-23 0.0 6.765110754334204E-5 0.0 2.300137656473629E-4 2.7060443017336813E-5 24-25 0.0 6.765110754334204E-5 0.0 3.3825553771671017E-4 2.7060443017336813E-5 26-27 0.0 6.765110754334204E-5 0.0 6.359204109074152E-4 2.7060443017336813E-5 28-29 0.0 6.765110754334204E-5 0.0 0.0018604054574419058 2.7060443017336813E-5 30-31 0.0 6.765110754334204E-5 0.0 0.005696223255149399 2.7060443017336813E-5 32-33 0.0 6.765110754334204E-5 0.0 0.013861711935630783 2.7060443017336813E-5 34-35 0.0 6.765110754334204E-5 0.0 0.027337812558264514 2.7060443017336813E-5 36-37 0.0 6.765110754334204E-5 0.0 0.04971003382284773 2.7060443017336813E-5 38-39 0.0 6.765110754334204E-5 0.0 0.0834273458224494 2.7060443017336813E-5 40-41 0.0 6.765110754334204E-5 0.0 0.11980334634850441 2.7060443017336813E-5 42-43 0.0 6.765110754334204E-5 0.0 0.1589056865085561 2.7060443017336813E-5 44-45 0.0 6.765110754334204E-5 0.0 0.20540229272309507 2.7060443017336813E-5 46-47 0.0 6.765110754334204E-5 0.0 0.2652126369021638 2.7060443017336813E-5 48-49 0.0 8.118132905201045E-5 0.0 0.33151748740539333 2.7060443017336813E-5 50-51 0.0 8.118132905201045E-5 0.0 0.4019896461332927 2.7060443017336813E-5 52-53 0.0 8.118132905201045E-5 0.0 0.47274593951287414 2.7060443017336813E-5 54-55 0.0 8.118132905201045E-5 0.0 0.5517556680127433 2.7060443017336813E-5 56-57 0.0 8.794643980634465E-5 0.0 0.6491664977644016 2.7060443017336813E-5 58-59 0.0 9.471155056067884E-5 0.0 0.7586327548802833 2.7060443017336813E-5 60-61 0.0 9.471155056067884E-5 0.0 0.8754323920538634 2.7060443017336813E-5 62-63 0.0 1.0147666131501305E-4 0.0 0.9931453191792785 2.7060443017336813E-5 64-65 0.0 1.1500688282368146E-4 0.0 1.1199505551585187 2.7060443017336813E-5 66-67 0.0 1.2177199357801566E-4 0.0 1.2691956635098856 2.7060443017336813E-5 68-69 0.0 1.2177199357801566E-4 0.0 1.4417601086314424 2.7060443017336813E-5 70-71 0.0 1.2177199357801566E-4 0.0 1.6343628118073372 2.7060443017336813E-5 72-73 0.0 1.2177199357801566E-4 0.0 1.8328646915610114 2.7060443017336813E-5 74-75 0.0 1.2177199357801566E-4 0.0 2.0329360770096914 2.7060443017336813E-5 76-77 0.0 1.2177199357801566E-4 0.0 2.256008839023107 2.7060443017336813E-5 78-79 0.0 1.2177199357801566E-4 0.0 2.510113164066654 2.7060443017336813E-5 80-81 0.0 1.2177199357801566E-4 0.0 2.7907570185994546 2.7060443017336813E-5 82-83 0.0 1.2177199357801566E-4 0.0 3.085695552156163 2.7060443017336813E-5 84-85 0.0 1.2177199357801566E-4 0.0 3.3799575746374373 2.7060443017336813E-5 86-87 0.0 1.2177199357801566E-4 0.0 3.6944134527204 2.7060443017336813E-5 88-89 0.0 1.2177199357801566E-4 0.0 4.045989493512394 2.7060443017336813E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7025 0.0 43.472 1 GTATCAA 11895 0.0 33.924763 1 ATCAACG 12260 0.0 31.28397 3 TCAACGC 12270 0.0 31.219788 4 CAACGCA 13640 0.0 28.080084 5 TATCAAC 14540 0.0 26.736755 2 AACGCAG 14840 0.0 25.856264 6 GTACATG 26790 0.0 23.67027 1 TACATGG 26380 0.0 23.103588 2 ACGCAGA 16650 0.0 22.62077 7 CGCAGAG 16310 0.0 22.252968 8 ACATGGG 26495 0.0 22.01856 3 CATGGGT 6735 0.0 18.888529 4 CATGGGG 14770 0.0 18.029486 4 GAGTACA 20480 0.0 17.773022 1 GCAGAGT 20235 0.0 17.186255 9 AGTACAT 21495 0.0 15.59037 2 AGAGTAC 17970 0.0 15.503483 5 CAGAGTA 19530 0.0 15.415526 10-11 TCTGTCG 1295 0.0 15.387213 8 >>END_MODULE