FastQCFastQC Report
Wed 25 May 2016
SRR2049427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390862
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85200.048.2586021
GTATCAA146400.034.4486771
ATCAACG161900.030.0142353
TCAACGC162700.029.9542354
CAACGCA173200.028.1110695
TATCAAC185200.026.623592
AACGCAG187350.026.164876
ACGCAGA217300.022.1870167
GTACATG273800.022.169491
CGCAGAG214550.022.139318
TACATGG264700.021.7499832
ACATGGG268550.020.55323
GCAGAGT255400.018.2262699
CATGGGG153850.017.9381284
GTGGTAT52100.017.4258481
TGGTATC47550.017.2824692
CAGAGTA246650.016.91816310-11
AGAGTAC226900.016.39154410-11
GAGTACA211750.016.319591
ATACCGC11750.016.1701767
Produced by FastQC (version 0.11.5)