FastQCFastQC Report
Wed 25 May 2016
SRR2049426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143771
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85610.11983866784083645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81050.036.320991
GTATCAA128700.030.3129081
ATCAACG130550.028.5812033
TCAACGC130450.028.5667234
CAACGCA141000.026.4259515
TATCAAC154050.024.6524492
GTACATG241700.024.5760481
AACGCAG153500.024.378736
TACATGG233800.024.1417712
ACATGGG242800.022.7582653
ACGCAGA172450.021.3721897
CGCAGAG168050.021.3423028
CATGGGT69400.019.5632674
CATGGGG143000.018.3247414
GCAGAGT198800.017.5161119
GAGTACA183200.017.2129291
AGAGTAC166000.015.634624510-11
GTATTAG40000.015.48123551
TACCTGG48600.015.3354032
CGATACA14250.015.3240922
Produced by FastQC (version 0.11.5)