##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049426_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7143771 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01171342138487 31.0 31.0 33.0 28.0 34.0 2 31.15023451899564 31.0 31.0 34.0 28.0 34.0 3 31.213828522778794 31.0 31.0 34.0 28.0 34.0 4 34.77676748036856 37.0 35.0 37.0 32.0 37.0 5 34.97474219148402 37.0 35.0 37.0 32.0 37.0 6 35.16700185378283 37.0 35.0 37.0 32.0 37.0 7 35.05002204018018 37.0 35.0 37.0 32.0 37.0 8 35.05151130964305 37.0 35.0 37.0 32.0 37.0 9 36.74177363748082 39.0 37.0 39.0 32.0 39.0 10-11 36.70222246765749 39.0 37.0 39.0 32.0 39.0 12-13 36.749952161120504 39.0 37.0 39.0 32.0 39.0 14-15 37.86667188799865 40.0 37.5 41.0 32.0 41.0 16-17 37.84695471061433 40.0 37.0 41.0 32.0 41.0 18-19 37.868703728045034 40.0 37.5 41.0 32.0 41.0 20-21 37.94548698439522 40.0 38.0 41.0 32.0 41.0 22-23 37.80284810361363 40.0 37.0 41.0 32.0 41.0 24-25 37.7940904040737 40.0 37.0 41.0 31.5 41.0 26-27 37.77282425486483 40.0 37.0 41.0 32.0 41.0 28-29 37.44058726126579 40.0 37.0 41.0 31.0 41.0 30-31 37.48553397078378 40.0 37.0 41.0 31.0 41.0 32-33 37.45194708788958 40.0 37.0 41.0 30.5 41.0 34-35 37.54859765241635 40.0 37.0 41.0 31.0 41.0 36-37 37.580283788492096 40.0 37.0 41.0 31.0 41.0 38-39 37.36195869100507 40.0 37.0 41.0 30.0 41.0 40-41 37.497075564152325 40.0 37.0 41.0 31.0 41.0 42-43 37.1823543895794 40.0 36.0 41.0 30.0 41.0 44-45 37.17990617280425 40.0 36.0 41.0 30.0 41.0 46-47 37.12590850126635 39.0 36.0 41.0 30.0 41.0 48-49 37.0039067601691 39.0 35.5 41.0 30.0 41.0 50-51 36.91250384425817 39.0 35.0 41.0 30.0 41.0 52-53 36.78290408525133 39.0 35.0 40.5 30.0 41.0 54-55 36.61172824548827 39.0 35.0 40.0 30.0 41.0 56-57 36.40309809762939 39.0 35.0 40.0 29.5 41.0 58-59 36.155760172043585 38.0 34.5 40.0 29.0 41.0 60-61 35.91764671627912 38.0 34.0 40.0 28.5 41.0 62-63 35.66183105533478 38.0 34.0 40.0 28.0 41.0 64-65 35.36721760817921 37.5 34.0 40.0 28.0 41.0 66-67 35.09587142420999 37.0 34.0 40.0 28.0 41.0 68-69 34.78455097454831 37.0 33.0 39.5 28.0 41.0 70-71 34.442853151367814 36.0 33.0 39.0 27.0 41.0 72-73 34.072855498867476 36.0 33.0 39.0 26.0 40.0 74-75 33.68238315589903 35.0 32.0 38.5 26.0 40.0 76-77 33.315641556819216 35.0 32.0 37.5 26.0 39.5 78-79 33.09784811131264 35.0 32.0 37.0 26.0 39.0 80-81 32.950447179787815 35.0 32.0 37.0 26.0 39.0 82-83 32.62607116045573 35.0 32.0 36.5 26.0 39.0 84-85 32.284870343688226 35.0 32.0 36.0 26.0 37.5 86-87 31.843506251810144 35.0 32.0 36.0 25.0 37.0 88-89 31.401494532789478 34.5 31.0 35.0 24.0 37.0 90-91 31.173388326697484 34.0 31.0 35.0 24.0 36.0 92-93 30.935330513814066 34.0 31.0 35.0 23.0 36.0 94-95 30.663325574126045 34.0 31.0 35.0 20.5 36.0 96-97 30.39266908191766 34.0 31.0 35.0 19.5 35.5 98-99 30.086266833021384 34.0 31.0 35.0 13.0 35.0 100-101 29.528177135017344 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19630273328202819 1101 2 0.3660088540382809 1101 3 0.21197494118149507 1101 4 0.24412816711294028 1101 5 0.23171756495771945 1101 6 0.14340450938702531 1101 7 0.17453283842605316 1101 8 0.32904446733748216 1101 9 0.30877149414345695 1101 10-11 0.32448001798254467 1101 12-13 0.3229272119856219 1101 14-15 0.5458826420854734 1101 16-17 0.4495525008878545 1101 18-19 0.4995149212496486 1101 20-21 0.5282411578407675 1101 22-23 0.46613494185447024 1101 24-25 0.09944055948694341 1101 26-27 0.3171161569381411 1101 28-29 0.8234476889120259 1101 30-31 0.44768790472853226 1101 32-33 0.7680085864613133 1101 34-35 0.5930473440519819 1101 36-37 0.557549590181381 1101 38-39 0.5606560766104352 1101 40-41 0.44167962986969656 1101 42-43 0.5043254452573862 1101 44-45 0.39191363588582817 1101 46-47 0.25068307083675023 1101 48-49 0.23131978609847437 1101 50-51 0.24349267987560808 1101 52-53 0.16854165732333826 1101 54-55 0.1361902814606779 1101 56-57 -0.014250550007652407 1101 58-59 0.02870435010694905 1101 60-61 0.059985937578950654 1101 62-63 -0.007696097701327176 1101 64-65 0.0501701740924716 1101 66-67 -0.011054361566195325 1101 68-69 -0.20035732671111361 1101 70-71 -0.16560362916141003 1101 72-73 -0.17608969810545005 1101 74-75 -0.14204046111554902 1101 76-77 -0.2792397503667772 1101 78-79 -0.22792388633197902 1101 80-81 -0.2820251442945292 1101 82-83 -0.4319406018538956 1101 84-85 -0.3635762701569121 1101 86-87 -0.3876794632199747 1101 88-89 -0.45362387390497005 1101 90-91 -0.5312228074514351 1101 92-93 -0.6543330432848329 1101 94-95 -0.6930006817325385 1101 96-97 -0.8266442818687416 1101 98-99 -0.78796788083217 1101 100-101 -0.8952102998575242 1102 1 0.013727795042598245 1102 2 0.08149881614459176 1102 3 -0.06548273904306257 1102 4 -0.05775983929905237 1102 5 0.05196856641642711 1102 6 -0.08443588232513832 1102 7 -0.11593961780457107 1102 8 0.105873632261968 1102 9 0.13520127296974493 1102 10-11 0.2156619776613482 1102 12-13 0.2740376399682276 1102 14-15 0.4349558223574448 1102 16-17 0.470379668902396 1102 18-19 0.46082859194352466 1102 20-21 0.3757851767171161 1102 22-23 -0.22864989164949634 1102 24-25 0.3354853362650232 1102 26-27 0.5204363735138031 1102 28-29 0.5676690446717672 1102 30-31 0.46115536066338336 1102 32-33 0.7043890874987611 1102 34-35 0.1386686348653967 1102 36-37 0.3244428197980298 1102 38-39 0.43253832917231705 1102 40-41 0.32409618478469326 1102 42-43 0.3213925822691479 1102 44-45 0.04720766203112703 1102 46-47 0.01816876687254876 1102 48-49 -0.1711921605738027 1102 50-51 -0.06342830585899151 1102 52-53 0.03963862119478989 1102 54-55 2.3026906706036243E-4 1102 56-57 -0.14881948568311287 1102 58-59 -0.12781361217970044 1102 60-61 -0.1049000535308906 1102 62-63 -0.21458253190053256 1102 64-65 -0.26988136894508585 1102 66-67 -0.3254397872760819 1102 68-69 -0.3287158081897559 1102 70-71 -0.4239428821595652 1102 72-73 -0.5132351167023543 1102 74-75 -0.5535574001613028 1102 76-77 -0.5356805179997792 1102 78-79 -0.515123035964649 1102 80-81 -0.5452747044061397 1102 82-83 -0.545466856952828 1102 84-85 -0.5202160047661479 1102 86-87 -0.5587876317065508 1102 88-89 -0.6966701175063896 1102 90-91 -0.6924175994306125 1102 92-93 -0.7689301140989748 1102 94-95 -0.8460276306152501 1102 96-97 -0.9090088003289161 1102 98-99 -1.002103571170334 1102 100-101 -1.1536794159392905 1103 1 0.15665766936671943 1103 2 0.10290819836955833 1103 3 0.06621564217779508 1103 4 0.0627518947315906 1103 5 -0.12057727557250786 1103 6 -0.06395134914892964 1103 7 -0.07171281500719573 1103 8 0.09240017680954082 1103 9 0.20134242863909435 1103 10-11 0.2897359603857197 1103 12-13 0.28250071036708135 1103 14-15 0.38953876136560694 1103 16-17 0.41237457176691095 1103 18-19 0.4244658807985999 1103 20-21 0.4113560683565254 1103 22-23 -0.009060211922310657 1103 24-25 0.29595834682466204 1103 26-27 0.5104164866594871 1103 28-29 0.42024714638743177 1103 30-31 0.48625073197187874 1103 32-33 0.5488361635552366 1103 34-35 0.5150258195139656 1103 36-37 0.3421158175321679 1103 38-39 0.5902214506505103 1103 40-41 0.33492173988244645 1103 42-43 -0.005972375537695029 1103 44-45 0.19032658579320128 1103 46-47 0.10169540481161476 1103 48-49 0.013033075366365665 1103 50-51 0.07228505946298469 1103 52-53 0.0035661336008274702 1103 54-55 -0.00368424918983834 1103 56-57 -0.035760139158227844 1103 58-59 -0.09419304280092433 1103 60-61 -0.13949646731104792 1103 62-63 -0.09115301377728713 1103 64-65 -0.22838806703467895 1103 66-67 -0.32788891407899 1103 68-69 -0.27892747056692 1103 70-71 -0.33702618650109173 1103 72-73 -0.4307247861438128 1103 74-75 -0.3060805452186841 1103 76-77 -0.30261537466984123 1103 78-79 -0.31437840549196494 1103 80-81 -0.36514296207892727 1103 82-83 -0.40687792035791404 1103 84-85 -0.47114352690462624 1103 86-87 -0.42579141876142756 1103 88-89 -0.3265096181332332 1103 90-91 -0.4213202804329512 1103 92-93 -0.5239430905830496 1103 94-95 -0.5885497204354628 1103 96-97 -0.5584860529686217 1103 98-99 -0.6968813287573568 1103 100-101 -0.658569186984824 1104 1 0.22184065409609843 1104 2 0.3601449419692102 1104 3 0.19484781391837203 1104 4 0.24085738419560698 1104 5 0.11350452590389892 1104 6 0.08487090625573757 1104 7 0.09457317430226908 1104 8 0.16477242539044568 1104 9 0.32330006769155517 1104 10-11 0.26434226364864344 1104 12-13 0.32460014240260904 1104 14-15 0.5222197330099903 1104 16-17 0.578914410406675 1104 18-19 0.43186671198908044 1104 20-21 0.4401966192024531 1104 22-23 0.6276297626178788 1104 24-25 0.7117310007030326 1104 26-27 0.6452389589534988 1104 28-29 0.5829747803860954 1104 30-31 0.7329960706153003 1104 32-33 0.7723743795671467 1104 34-35 0.611008988092351 1104 36-37 0.3665923690472539 1104 38-39 0.49583205512138306 1104 40-41 0.426924772788027 1104 42-43 0.4596398494736391 1104 44-45 0.26426638670760383 1104 46-47 0.1793074064063802 1104 48-49 0.23379520467767634 1104 50-51 0.18018356571962357 1104 52-53 0.16760432709879325 1104 54-55 0.14663907281936872 1104 56-57 0.13552033531057361 1104 58-59 0.1281658829560257 1104 60-61 0.16234008612442352 1104 62-63 0.18768258366853985 1104 64-65 0.20204253849711762 1104 66-67 0.04357453633892305 1104 68-69 0.048232881166235586 1104 70-71 0.023591728128067757 1104 72-73 0.008579959919224223 1104 74-75 0.12942251182437303 1104 76-77 0.03988093985138619 1104 78-79 2.9164140364201785E-4 1104 80-81 -0.062140789938432306 1104 82-83 -0.02522531764766711 1104 84-85 -0.08056274355902104 1104 86-87 0.02527873765237132 1104 88-89 0.025321619094579972 1104 90-91 0.021544117273851526 1104 92-93 -0.008250644511576866 1104 94-95 -0.016139314935553983 1104 96-97 -0.10938009778559632 1104 98-99 -0.11670618696669521 1104 100-101 -0.17460065933181212 1105 1 0.18877966096174603 1105 2 0.23515022947701425 1105 3 0.11806515952692465 1105 4 0.17609119666671802 1105 5 0.08901476172204781 1105 6 0.13856884310391138 1105 7 0.10779574468374875 1105 8 -0.07485376017098133 1105 9 -0.13127466158089618 1105 10-11 0.1962092670210822 1105 12-13 0.30115094953888644 1105 14-15 0.3726929767083291 1105 16-17 0.0823645628892038 1105 18-19 0.23990135034541282 1105 20-21 0.19822796551044775 1105 22-23 0.47780783566326335 1105 24-25 0.2225219049962135 1105 26-27 0.0013871238521403484 1105 28-29 0.5221904182982229 1105 30-31 0.4454844774351514 1105 32-33 0.5159902937334095 1105 34-35 -0.20376490846965112 1105 36-37 -0.3264795223814474 1105 38-39 -0.10893441502096124 1105 40-41 -0.018060874616878664 1105 42-43 0.20957024850136463 1105 44-45 0.07153591502185463 1105 46-47 -0.13107648954707685 1105 48-49 -0.18649424095814737 1105 50-51 -0.28640964835314264 1105 52-53 -0.03092258651005153 1105 54-55 -0.1933042600144006 1105 56-57 -0.42026487461769335 1105 58-59 -0.04803947673570974 1105 60-61 -0.09002608128025713 1105 62-63 -0.14899371814857432 1105 64-65 -0.3885911436162388 1105 66-67 -0.32638758242256927 1105 68-69 -0.33826382623368545 1105 70-71 -0.051016049140969244 1105 72-73 -0.11569452248843248 1105 74-75 0.015590142108258931 1105 76-77 -0.0846005676443724 1105 78-79 0.004098795173845815 1105 80-81 -0.05238815293822796 1105 82-83 -0.08405982055388961 1105 84-85 -0.1968935756576471 1105 86-87 -0.097912914244354 1105 88-89 -0.25015228566995873 1105 90-91 -0.08865680740959903 1105 92-93 -0.08643272085938847 1105 94-95 -0.04520660489398054 1105 96-97 -0.150209272600474 1105 98-99 -0.15171874048087375 1105 100-101 -0.252372093008443 1106 1 0.2795415695447758 1106 2 0.3004911789413498 1106 3 0.240393653090905 1106 4 0.3070030108771107 1106 5 0.2718393383838844 1106 6 0.10989424807318926 1106 7 0.1677631384451459 1106 8 0.16660433651757245 1106 9 0.26290379240695927 1106 10-11 0.004524010972055237 1106 12-13 0.20850115638608457 1106 14-15 0.45645378535686376 1106 16-17 0.3557262756683528 1106 18-19 0.3699266667404757 1106 20-21 0.34644004589886634 1106 22-23 0.5023720644493253 1106 24-25 0.4311772074004736 1106 26-27 0.47461246563937465 1106 28-29 0.46401951616270765 1106 30-31 0.5394098459885797 1106 32-33 0.5630894687393635 1106 34-35 0.46215967798317337 1106 36-37 0.3046318016832501 1106 38-39 0.3929255864567054 1106 40-41 0.25035318417751995 1106 42-43 -0.5355826474183303 1106 44-45 0.12690057679376565 1106 46-47 0.1221205066872102 1106 48-49 0.04157113844467375 1106 50-51 0.17156817637387434 1106 52-53 0.06538337586668064 1106 54-55 0.006627648709176981 1106 56-57 -0.10131861484643423 1106 58-59 -0.07153897576262125 1106 60-61 0.06266337986490811 1106 62-63 -0.036075125314113166 1106 64-65 0.10729314121788036 1106 66-67 0.03799512301741714 1106 68-69 -0.02734091885140799 1106 70-71 0.043061012855403646 1106 72-73 0.021184616938647594 1106 74-75 0.04481651026024025 1106 76-77 -0.09756671582357512 1106 78-79 -0.049682860723393674 1106 80-81 -0.07096473060696695 1106 82-83 -0.04490167362277475 1106 84-85 -0.05118199325870876 1106 86-87 0.01134673185296009 1106 88-89 -0.02120375520803819 1106 90-91 0.02058285629577128 1106 92-93 0.04107638447671036 1106 94-95 0.20560954233911133 1106 96-97 0.19376885888963358 1106 98-99 0.17346949443636817 1106 100-101 0.13877335579961425 1107 1 0.1947255744308869 1107 2 9.928887103676232E-4 1107 3 0.09017853127063447 1107 4 0.10083774510869858 1107 5 0.010169903167934535 1107 6 -0.06887781822915429 1107 7 0.05653461368581958 1107 8 -0.4440654040802343 1107 9 -0.18500192525273462 1107 10-11 -0.45870566543399605 1107 12-13 -0.252471387875822 1107 14-15 -0.05653437155276464 1107 16-17 0.15171145079499126 1107 18-19 0.12271316849273006 1107 20-21 -0.3821792448148216 1107 22-23 0.11590958547510866 1107 24-25 0.31857955139097527 1107 26-27 -0.11973801607588541 1107 28-29 -0.3118415406309225 1107 30-31 -0.07621980246518234 1107 32-33 -0.3926998494833214 1107 34-35 -0.28677446389379213 1107 36-37 -0.8384018054060434 1107 38-39 -0.3451271947347223 1107 40-41 -0.4159131177343198 1107 42-43 -0.32102730742241903 1107 44-45 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0.25827201212805306 2214 56-57 0.19687703342970764 2214 58-59 0.16898630707945728 2214 60-61 0.12125298809252172 2214 62-63 0.2640968609044805 2214 64-65 0.19051155160244804 2214 66-67 0.2834509617774472 2214 68-69 0.41312447456986234 2214 70-71 0.3412129846655887 2214 72-73 0.39206824627842707 2214 74-75 0.3580664301049623 2214 76-77 0.30667870274950104 2214 78-79 0.3760883075414796 2214 80-81 0.3450123801997833 2214 82-83 0.35661196288387487 2214 84-85 0.31379234853776694 2214 86-87 0.216037715780935 2214 88-89 0.31840682280553523 2214 90-91 0.2610338939146679 2214 92-93 0.14695929377816697 2214 94-95 0.07833128597176042 2214 96-97 0.18946422331302415 2214 98-99 0.34905478394647815 2214 100-101 0.40787797407731574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 83.0 11 1754.0 12 7300.0 13 14531.0 14 20009.0 15 23357.0 16 25309.0 17 26088.0 18 27236.0 19 28584.0 20 29995.0 21 32766.0 22 37607.0 23 47197.0 24 58830.0 25 68575.0 26 79379.0 27 90627.0 28 106939.0 29 128447.0 30 156125.0 31 192551.0 32 245479.0 33 316200.0 34 428865.0 35 609476.0 36 917955.0 37 1376334.0 38 1577499.0 39 468299.0 40 375.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50658001483066 21.229128042232226 13.680874272070307 23.58341767086681 2 14.699491771847711 21.16209019456281 40.55095007888666 23.587467954702824 3 17.714134906283213 26.612365379693664 30.43827795271653 25.23522176130659 4 12.170066856735806 17.755145962226422 39.96304076248849 30.111746418549284 5 12.351463947462134 38.79978414623588 35.90820107013482 12.94055083616716 6 28.862043715615986 39.34177267638918 18.68944980397586 13.106733804018974 7 25.808223596349322 33.568821985585664 23.435950502306728 17.187003915758282 8 24.13528053190118 37.03721849626799 21.2702658400027 17.557235131828136 9 24.26959062354354 17.90620715492834 22.849491532732323 34.9747106887958 10-11 22.878811495693093 27.855727174490703 29.89377425694509 19.371687072871115 12-13 23.337771170020943 25.310928557829072 29.865471950632216 21.48582832151777 14-15 20.773889553704773 27.527171432715253 27.188392986637293 24.510546026942684 16-17 20.280197620855027 30.990578175966455 27.850901202733024 20.878323000445498 18-19 20.26653725627143 30.001169649342486 29.62222006164913 20.110073032736953 20-21 21.53689919281572 29.069167945900652 29.070742782558597 20.323190078725034 22-23 21.268329130344636 28.91789689193501 29.08126115700651 20.732512820713843 24-25 20.963329277813912 29.094023753249846 28.97228115370927 20.97036581522697 26-27 20.641172428303317 29.258465023452455 29.067300931281014 21.03306161696321 28-29 20.722031116115193 29.10808019765098 29.280327671263713 20.88956101497011 30-31 20.944240079382162 29.48942003974008 28.863915265968664 20.702424614909102 32-33 20.58296073979299 29.21556577757495 29.405028542951612 20.79644493968044 34-35 20.892110936044485 29.097736661734768 29.117418533533968 20.89273386868678 36-37 21.176133147613633 29.119366807906864 28.80588957563618 20.89861046884332 38-39 20.784149068570194 29.290187471589928 29.115097013091457 20.810566446748417 40-41 20.66946992562962 29.05243122739863 29.314273430932925 20.96382541603883 42-43 20.71610546095386 29.0366893685538 29.166913395877263 21.08029177461507 44-45 20.72665969813953 29.172476786411888 29.10056791419542 21.000295601253157 46-47 20.944475833908236 29.28583307947999 28.87639656670818 20.893294519903595 48-49 20.868193968723915 29.03184755464327 29.076443874995345 21.023514601637473 50-51 20.804943296460586 29.03542662196315 29.056636898545158 21.102993183031106 52-53 20.778523888311753 29.113997415807418 29.011184098206876 21.096294597673946 54-55 20.86284016827943 29.387197665333588 28.91377702093441 20.836185145452568 56-57 20.813024269241176 29.162203770685707 28.78923142806465 21.235540532008464 58-59 20.836143307492073 28.833022923649015 29.14106422617407 21.189769542684843 60-61 20.93942557492875 29.099136239165713 28.925175799799746 21.03626238610579 62-63 20.758840636844482 29.11339259990139 28.990525674168644 21.13724108908549 64-65 20.849053783873973 29.013514413136278 28.947040331832792 21.19039147115696 66-67 20.676211990926852 28.97919789260117 28.98129089938251 21.363299217089462 68-69 20.965963807225535 29.153757624856702 28.849113337642297 21.031165230275466 70-71 20.983486883225325 28.890808213933504 28.813467502552943 21.312237400288225 72-73 20.76022386866073 28.915750359353932 29.135349121923444 21.188676650061897 74-75 20.790502893607602 29.05915523640737 28.98423106187009 21.16611080811494 76-77 20.846226677166165 28.99968327880091 28.844767127307918 21.30932291672501 78-79 20.85414339772159 28.937067005111324 29.066601889890446 21.14218770727664 80-81 20.7694397245495 28.898415025438425 29.060406020648987 21.27173922936309 82-83 20.677383075780902 28.82013073794082 29.289866258368264 21.21261992791002 84-85 20.661857477535257 28.747428649846874 29.083065987601508 21.50764788501636 86-87 20.80723610726155 28.81222963601578 29.011185498790248 21.36934875793242 88-89 20.741278825105468 28.775279840845354 29.075860528250942 21.40758080579824 90-91 20.63964684505872 28.65952152897092 29.123493734723787 21.57733789124657 92-93 20.759964083962508 28.77308024110144 28.981691314630787 21.48526436030527 94-95 20.735010499365277 28.55790031286123 29.18000302255522 21.527086165218275 96-97 20.745736048169338 28.67931131733674 29.018420525416467 21.55653210907745 98-99 20.688911091669006 28.522898430450304 29.09490054631093 21.693289931569755 100-101 20.79664809984886 28.57638818223936 28.91304716767944 21.713916550232344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7488.0 1 4468.5 2 1081.5 3 564.0 4 347.0 5 226.0 6 178.0 7 254.5 8 366.0 9 398.5 10 292.5 11 175.5 12 178.5 13 225.5 14 294.5 15 452.0 16 737.0 17 1266.0 18 2168.5 19 3536.5 20 5801.0 21 9482.5 22 15646.0 23 25913.0 24 37516.0 25 49880.5 26 67515.0 27 89234.5 28 116570.5 29 143419.5 30 161695.0 31 180514.0 32 202525.0 33 220875.0 34 236173.0 35 250301.0 36 259410.0 37 266013.0 38 271431.5 39 273780.5 40 275086.5 41 269590.5 42 260492.5 43 253632.5 44 246108.0 45 238078.0 46 230238.5 47 222845.0 48 215481.0 49 208641.0 50 200475.0 51 186421.5 52 169103.0 53 155227.5 54 146073.5 55 139307.0 56 125741.0 57 110054.0 58 98731.0 59 87909.5 60 77040.5 61 64906.5 62 53406.0 63 43963.0 64 35789.0 65 29314.0 66 24078.5 67 19099.5 68 14460.5 69 10477.0 70 7711.5 71 5569.5 72 3990.5 73 2925.5 74 2260.5 75 1677.5 76 1118.0 77 775.0 78 542.0 79 331.5 80 195.0 81 106.0 82 56.0 83 27.5 84 17.0 85 10.5 86 7.0 87 3.5 88 2.5 89 2.5 90 3.0 91 2.5 92 1.0 93 1.0 94 1.0 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44062162686905837 2 0.0706629593809768 3 0.07113889848932727 4 0.09405396673549586 5 0.07845996183248315 6 1.1198567255305357E-4 7 0.004577414365606065 8 5.319319446270044E-4 9 0.014418155341205646 10-11 9.728755303046527E-4 12-13 0.009112834104004732 14-15 0.008496912904962938 16-17 0.016867841928303692 18-19 0.00846191738229011 20-21 0.002421690168959783 22-23 0.01173049919993236 24-25 0.03463856834156638 26-27 0.002379695541752388 28-29 0.003779516448665558 30-31 0.0014068200114477354 32-33 0.002113729569438886 34-35 0.0019807465832821346 36-37 4.269453766085167E-4 38-39 0.11641610572343375 40-41 0.007524037374658287 42-43 0.004367441229569089 44-45 0.009833741871065015 46-47 0.007545034688261984 48-49 0.011268558300651014 50-51 0.014096196532615617 52-53 0.010183697097793308 54-55 0.009126832313073865 56-57 0.00859490036844686 58-59 0.014838101613279597 60-61 0.0187925956753093 62-63 0.009476787539802158 64-65 0.015922962816137302 66-67 0.013116321897776399 68-69 0.007307065134086745 70-71 7.838997078713749E-4 72-73 0.010407668442899415 74-75 0.01702882133259871 76-77 0.025798699314409715 78-79 0.01810668343092185 80-81 0.028220389483369496 82-83 0.059093439585339455 84-85 0.0361293776074289 86-87 0.35496658557504157 88-89 0.6230952811897246 90-91 0.6143044058943099 92-93 0.6220174190914015 94-95 0.6133735249912126 96-97 0.6075712673320575 98-99 0.616754092481408 100-101 0.609754987946842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7143771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.79947949295009 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69795398894743 40.56507992785701 2 15.784502994698506 17.61534102318177 3 5.312931569194567 8.893764484281581 4 2.3375971545664354 5.217468179560331 5 1.1623797774941176 3.243009327865145 6 0.6747825253026478 2.25915082096957 7 0.41735452747493007 1.6301715777989063 8 0.2717462388053474 1.2130638943604237 9 0.2204616933852733 1.1071482965129344 >10 1.0109469779716034 9.990048987230244 >50 0.0660301184729427 2.544324887465107 >100 0.04033616429712648 4.146309309585234 >500 0.0021944460847535675 0.8214846250862091 >1k 7.566031988053466E-4 0.6331583798203766 >5k 2.5220105481338022E-5 0.12047627842511224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8561 0.11983866784083645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.3998209069131696E-5 0.0 0.0 0.0 3 0.0 1.3998209069131696E-5 0.0 1.3998209069131696E-5 0.0 4 0.0 2.799641813826339E-5 0.0 2.799641813826339E-5 0.0 5 0.0 2.799641813826339E-5 0.0 2.799641813826339E-5 0.0 6 0.0 2.799641813826339E-5 0.0 2.799641813826339E-5 0.0 7 0.0 2.799641813826339E-5 0.0 5.599283627652678E-5 0.0 8 0.0 2.799641813826339E-5 0.0 5.599283627652678E-5 0.0 9 0.0 2.799641813826339E-5 0.0 6.999104534565848E-5 0.0 10-11 0.0 2.799641813826339E-5 0.0 6.999104534565848E-5 0.0 12-13 0.0 2.799641813826339E-5 0.0 7.699014988022433E-5 0.0 14-15 0.0 4.899373174196094E-5 0.0 8.398925441479018E-5 0.0 16-17 0.0 6.299194081109263E-5 0.0 1.0498656801848771E-4 0.0 18-19 0.0 7.699014988022433E-5 0.0 1.1198567255305357E-4 0.0 20-21 0.0 8.398925441479018E-5 0.0 1.3298298615675112E-4 0.0 22-23 0.0 8.398925441479018E-5 0.0 2.1697224057154127E-4 0.0 24-25 0.0 1.0498656801848771E-4 0.0 3.849507494011216E-4 0.0 26-27 0.0 1.2598388162218525E-4 0.0 6.089220945072288E-4 0.0 28-29 0.0 1.3998209069131695E-4 0.0 0.0016937832973649351 0.0 30-31 0.0 1.3998209069131695E-4 0.0 0.004661403620020855 0.0 32-33 0.0 1.3998209069131695E-4 0.0 0.011219564568909053 0.0 34-35 0.0 1.469811952258828E-4 0.0 0.021592237489135642 0.0 36-37 0.0 2.239713451061071E-4 0.0 0.03960793256110813 6.999104534565848E-6 38-39 0.0 2.939623904517656E-4 0.0 0.06703742323207168 1.3998209069131696E-5 40-41 0.0 3.009614949863314E-4 0.0 0.09846340259227235 1.3998209069131696E-5 42-43 0.0 3.21958808590029E-4 0.0 0.12988238284793843 1.3998209069131696E-5 44-45 0.0 3.21958808590029E-4 0.0 0.1680904945021334 1.3998209069131696E-5 46-47 0.0 3.21958808590029E-4 0.0 0.21646130594051796 1.3998209069131696E-5 48-49 0.0 3.2895791312459487E-4 0.0 0.2692625505492827 1.3998209069131696E-5 50-51 0.0 3.4295612219372655E-4 0.0 0.3263612453422709 1.3998209069131696E-5 52-53 0.0 3.499552267282924E-4 0.0 0.38465678701066985 1.3998209069131696E-5 54-55 0.0 3.569543312628582E-4 0.0 0.449832448436547 1.3998209069131696E-5 56-57 0.0 3.6395343579742406E-4 0.0 0.5317499679091058 1.3998209069131696E-5 58-59 0.0 3.6395343579742406E-4 0.0 0.6243341226923427 1.3998209069131696E-5 60-61 0.0 3.779516448665558E-4 0.0 0.723007498420652 1.3998209069131696E-5 62-63 0.0 3.779516448665558E-4 0.0 0.8236266252095708 1.3998209069131696E-5 64-65 0.0 3.779516448665558E-4 0.0 0.9344434473053518 1.3998209069131696E-5 66-67 0.0 3.849507494011216E-4 0.0 1.0656276635967195 1.3998209069131696E-5 68-69 0.0 4.12947167539385E-4 0.0 1.2158284469085026 1.3998209069131696E-5 70-71 0.0 4.3394448114308253E-4 0.0 1.3807483470564774 1.3998209069131696E-5 72-73 0.0 4.4794269021221426E-4 0.0 1.5526883490526222 1.3998209069131696E-5 74-75 0.0 4.549417947467801E-4 0.0 1.7319424152873881 1.3998209069131696E-5 76-77 0.0 5.179337355578727E-4 0.0 1.9271124452337567 1.3998209069131696E-5 78-79 0.0 5.179337355578727E-4 0.0 2.1537784455856714 1.3998209069131696E-5 80-81 0.0 5.249328400924386E-4 0.0 2.4033105204520133 1.3998209069131696E-5 82-83 0.0 5.319319446270045E-4 0.0 2.66888454291158 1.3998209069131696E-5 84-85 0.0 5.319319446270045E-4 0.0 2.9356064185148156 1.3998209069131696E-5 86-87 0.0 5.319319446270045E-4 0.0 3.2223527321914434 1.3998209069131696E-5 88-89 0.0 5.319319446270045E-4 0.0 3.5414279097132315 1.3998209069131696E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8105 0.0 36.32099 1 GTATCAA 12870 0.0 30.312908 1 ATCAACG 13055 0.0 28.581203 3 TCAACGC 13045 0.0 28.566723 4 CAACGCA 14100 0.0 26.425951 5 TATCAAC 15405 0.0 24.652449 2 GTACATG 24170 0.0 24.576048 1 AACGCAG 15350 0.0 24.37873 6 TACATGG 23380 0.0 24.141771 2 ACATGGG 24280 0.0 22.758265 3 ACGCAGA 17245 0.0 21.372189 7 CGCAGAG 16805 0.0 21.342302 8 CATGGGT 6940 0.0 19.563267 4 CATGGGG 14300 0.0 18.324741 4 GCAGAGT 19880 0.0 17.516111 9 GAGTACA 18320 0.0 17.212929 1 AGAGTAC 16600 0.0 15.6346245 10-11 GTATTAG 4000 0.0 15.4812355 1 TACCTGG 4860 0.0 15.335403 2 CGATACA 1425 0.0 15.324092 2 >>END_MODULE