FastQCFastQC Report
Wed 25 May 2016
SRR2049426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143771
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97550.040.148461
GTATCAA155850.032.3576971
ATCAACG166650.029.3295483
TCAACGC166600.029.3098414
CAACGCA176550.027.6856775
AACGCAG192550.025.6314666
TATCAAC192500.025.5392422
GTACATG251550.023.1462571
TACATGG246100.022.6790392
CGCAGAG219300.022.0501028
ACGCAGA224800.021.806447
ACATGGG249800.021.4112153
GCAGAGT254900.018.5046489
CATGGGT73350.018.0675564
TACCTGG48200.017.3445872
GAGTACA194000.017.0520461
CATGGGG144800.016.9268634
CTGTCGC15500.016.8551439
GTGGTAT62900.016.775341
TCTGTCG13950.016.6848898
Produced by FastQC (version 0.11.5)