FastQCFastQC Report
Wed 25 May 2016
SRR2049425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156549
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171550.27864636503339774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.049.3808441
GTATCAA131000.036.7595981
TCAACGC150300.031.550164
ATCAACG151300.031.4671233
CAACGCA164750.028.7780235
TATCAAC170900.027.9691282
AACGCAG179900.026.4711596
GTACATG308200.025.3455721
TACATGG299900.024.3111932
CGCAGAG194350.023.4591128
GTGGTAT40350.023.254851
ACGCAGA201750.023.1829687
ACATGGG307750.023.0588783
TGGTATC39150.022.3088682
TCTGTCG9100.021.8967978
CATGGGT69550.020.7477724
GTACAGG42900.019.8740621
GCAGAGT225350.019.64259
CATGGGG166600.018.9185624
TACCTGG44100.018.7284662
Produced by FastQC (version 0.11.5)