FastQCFastQC Report
Wed 25 May 2016
SRR2049425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156549
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.15497318384049247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84260.13686238832826636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95800.052.14621
GTATCAA167700.038.8304941
ATCAACG202100.031.6584993
TCAACGC204350.031.2169484
CAACGCA213500.029.9245455
TATCAAC221500.029.0367552
AACGCAG232200.027.5964286
GTGGTAT46600.026.417961
TGGTATC46650.025.5579032
CGCAGAG260300.024.0151258
ACGCAGA269200.023.538787
GTACATG316700.023.2331621
TACATGG303850.022.5741332
ACATGGG309350.021.3889793
CATGGGT69600.021.0198944
GCAGAGT292950.020.9007859
TCTGTCG10850.020.5764888
TACGACG1200.004765209719.79215
CTGTCGC12100.018.8433919
TACCTGG43900.018.8272932
Produced by FastQC (version 0.11.5)