##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049425_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6156549 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.956807133346945 31.0 31.0 34.0 30.0 34.0 2 32.148062818959126 33.0 31.0 34.0 30.0 34.0 3 32.255583769413676 33.0 31.0 34.0 30.0 34.0 4 35.93283071408999 37.0 35.0 37.0 35.0 37.0 5 35.79745243642177 37.0 35.0 37.0 35.0 37.0 6 35.87660652095841 37.0 35.0 37.0 35.0 37.0 7 35.849288944179605 37.0 35.0 37.0 35.0 37.0 8 35.893405380189456 37.0 35.0 37.0 35.0 37.0 9 37.629709111386916 39.0 37.0 39.0 35.0 39.0 10-11 37.5569654363183 39.0 37.0 39.0 35.0 39.0 12-13 37.57606923943917 39.0 37.0 39.0 35.0 39.0 14-15 38.943503657649764 40.0 38.0 41.0 35.5 41.0 16-17 38.98456781550833 40.0 38.0 41.0 36.0 41.0 18-19 39.032998762780906 40.0 38.5 41.0 36.0 41.0 20-21 39.05611715264509 40.0 39.0 41.0 36.0 41.0 22-23 39.021397864290535 40.0 38.5 41.0 35.0 41.0 24-25 39.030366037856595 40.0 38.0 41.0 35.0 41.0 26-27 38.980006899969446 40.0 38.0 41.0 35.0 41.0 28-29 38.93778519427036 40.0 38.0 41.0 35.0 41.0 30-31 38.85084135609089 40.0 38.0 41.0 35.0 41.0 32-33 38.80481110440281 40.0 38.0 41.0 35.0 41.0 34-35 38.729948385044935 40.0 38.0 41.0 35.0 41.0 36-37 38.691572015426175 40.0 38.0 41.0 35.0 41.0 38-39 38.64836120040627 40.0 38.0 41.0 35.0 41.0 40-41 38.582970833172936 40.0 38.0 41.0 34.0 41.0 42-43 38.51827606667307 40.0 38.0 41.0 34.0 41.0 44-45 38.4656087363229 40.0 38.0 41.0 34.0 41.0 46-47 38.35319145514801 40.0 38.0 41.0 34.0 41.0 48-49 38.24858942891545 40.0 38.0 41.0 33.0 41.0 50-51 38.150476590050694 40.0 37.0 41.0 33.0 41.0 52-53 37.99671959079673 40.0 37.0 41.0 33.0 41.0 54-55 37.8404025534435 40.0 37.0 41.0 33.0 41.0 56-57 37.6663785994394 39.5 36.0 41.0 33.0 41.0 58-59 37.46237705571741 39.0 36.0 41.0 32.5 41.0 60-61 37.2199759150784 39.0 35.5 41.0 32.0 41.0 62-63 36.96652207267415 39.0 35.0 40.5 32.0 41.0 64-65 36.705622256884496 38.0 35.0 40.0 31.5 41.0 66-67 36.394804865518005 38.0 35.0 40.0 31.0 41.0 68-69 36.08600167074118 37.0 35.0 40.0 31.0 41.0 70-71 35.76516844095613 37.0 35.0 39.0 31.0 41.0 72-73 35.40243982464851 36.0 34.0 39.0 30.5 41.0 74-75 35.078319850942464 36.0 34.0 39.0 30.0 40.0 76-77 34.73190240181634 35.0 34.0 37.5 30.0 40.0 78-79 34.37270311663239 35.0 34.0 37.0 30.0 39.0 80-81 34.04044619802425 35.0 34.0 37.0 29.0 39.0 82-83 33.97076584625575 35.0 34.0 36.0 30.0 39.0 84-85 33.7619848392338 35.0 34.0 36.0 30.0 37.0 86-87 33.54119466928631 35.0 34.0 36.0 30.0 37.0 88-89 33.3046538734606 35.0 34.0 35.0 29.5 37.0 90-91 33.10036832322783 35.0 34.0 35.0 29.0 36.0 92-93 32.94051318360334 35.0 34.0 35.0 29.0 36.0 94-95 32.77718734960121 35.0 33.5 35.0 29.0 36.0 96-97 32.622420206515045 35.0 33.0 35.0 29.0 36.0 98-99 32.43283875430862 35.0 33.0 35.0 29.0 35.0 100-101 32.05165158272922 35.0 33.0 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10520518942463752 1101 2 0.1557586307443799 1101 3 0.1313522063670547 1101 4 0.08428583989660865 1101 5 0.11416881516347388 1101 6 0.18658543451388 1101 7 0.12428482641853833 1101 8 0.18854901964123627 1101 9 0.2587206245109144 1101 10-11 0.2925001931285678 1101 12-13 0.24026594942510116 1101 14-15 0.3176860647064501 1101 16-17 0.31919617205091555 1101 18-19 0.34778187926951176 1101 20-21 0.26874995940876545 1101 22-23 0.34026275096174885 1101 24-25 0.31865484653975784 1101 26-27 0.3477771886662424 1101 28-29 0.2652669380878834 1101 30-31 0.3342165096250227 1101 32-33 0.3428241225940596 1101 34-35 0.3647592546595533 1101 36-37 0.3669380230413566 1101 38-39 0.2973357357339168 1101 40-41 0.33882125714416134 1101 42-43 0.32075319937228386 1101 44-45 0.2815169256644481 1101 46-47 0.32061928231627235 1101 48-49 0.27804336769445115 1101 50-51 0.28316347698959277 1101 52-53 0.2965272407932744 1101 54-55 0.23866258691521836 1101 56-57 0.20483175368917017 1101 58-59 0.27043829456206225 1101 60-61 0.25465324979928283 1101 62-63 0.24188456860084528 1101 64-65 0.30353978358728995 1101 66-67 0.21249933993919257 1101 68-69 0.2170852315205849 1101 70-71 0.18925066035715332 1101 72-73 0.21718053778204904 1101 74-75 0.16127645520364098 1101 76-77 0.11165982938879893 1101 78-79 0.12801224072082817 1101 80-81 0.1754851804155777 1101 82-83 0.13608318628023142 1101 84-85 0.06827011198941335 1101 86-87 0.008086471596683964 1101 88-89 0.037228720273461136 1101 90-91 0.08474710365582894 1101 92-93 -0.013739024325403193 1101 94-95 -0.054582015837667086 1101 96-97 -0.012800814689839513 1101 98-99 -0.09833537174764473 1101 100-101 -0.10819398901607258 1102 1 0.03866010926951802 1102 2 0.08492002826540812 1102 3 0.05028396404264157 1102 4 -0.013644422006713341 1102 5 0.04518869569172068 1102 6 0.14650642184719942 1102 7 0.21256793492953108 1102 8 0.1872094842731329 1102 9 0.25873301993097186 1102 10-11 0.2732837699898525 1102 12-13 0.218280072733954 1102 14-15 0.1919172729056271 1102 16-17 0.20984610793655634 1102 18-19 0.15993281031626339 1102 20-21 0.1828350805384531 1102 22-23 0.21983655448318729 1102 24-25 0.20518438098154945 1102 26-27 0.3322213351109582 1102 28-29 0.1776438993463998 1102 30-31 0.20351604432825354 1102 32-33 0.13650766334809816 1102 34-35 0.08272834251324213 1102 36-37 0.20403526116921 1102 38-39 0.2614022648256267 1102 40-41 0.3264001944008186 1102 42-43 0.18256902924761675 1102 44-45 0.17995889016575717 1102 46-47 0.1749567696115122 1102 48-49 0.28489775103933823 1102 50-51 0.2609843245746575 1102 52-53 0.2975948523164007 1102 54-55 0.2747880284788522 1102 56-57 0.3467371108237174 1102 58-59 0.3360770565498825 1102 60-61 0.3175685031969451 1102 62-63 0.3823557210291497 1102 64-65 0.24809274893325295 1102 66-67 0.32865177577901505 1102 68-69 0.25317651196179725 1102 70-71 0.26913552230351456 1102 72-73 0.21174102884076262 1102 74-75 0.18673678451903442 1102 76-77 0.15373603909607425 1102 78-79 0.07681133092538772 1102 80-81 0.11956692868048435 1102 82-83 0.14747324494366154 1102 84-85 0.052981087597650856 1102 86-87 0.09526503350910787 1102 88-89 0.08631716654283395 1102 90-91 0.09171373380897307 1102 92-93 0.02125272229063313 1102 94-95 0.01405212397991562 1102 96-97 -0.01673915635387857 1102 98-99 0.006212619267103037 1102 100-101 -0.04938284281845995 1103 1 0.02090552737587359 1103 2 0.11479180182664095 1103 3 0.029562950615115824 1103 4 -0.032914507029239815 1103 5 0.04709432889440279 1103 6 0.017932457734048057 1103 7 0.13980181475017162 1103 8 0.1260048540758021 1103 9 0.18807134007120396 1103 10-11 0.1437162247276902 1103 12-13 0.03635841579882282 1103 14-15 0.16216742270664497 1103 16-17 0.12993525741035938 1103 18-19 0.14106370010613745 1103 20-21 0.17649788769000452 1103 22-23 0.22620216918664227 1103 24-25 0.16349808662838683 1103 26-27 0.1383319789576518 1103 28-29 0.20867791695187776 1103 30-31 0.23410002491800697 1103 32-33 0.27119328004254584 1103 34-35 0.29838977091870333 1103 36-37 0.15734553655735084 1103 38-39 0.12344186247268851 1103 40-41 0.15566234666256662 1103 42-43 0.2521059139147397 1103 44-45 0.1337853481816751 1103 46-47 0.21127279765940443 1103 48-49 0.2012843339579149 1103 50-51 0.1072201590065518 1103 52-53 0.06165862687473833 1103 54-55 0.11075932063242533 1103 56-57 0.1920760803242274 1103 58-59 0.11975451425321637 1103 60-61 0.1171923046569745 1103 62-63 0.07774993193375934 1103 64-65 0.12195209618568725 1103 66-67 0.07353463565900853 1103 68-69 0.006079631092639204 1103 70-71 -0.03156209902846285 1103 72-73 0.07391131877218271 1103 74-75 0.07241824331938318 1103 76-77 0.13772968180145995 1103 78-79 0.10145262478607009 1103 80-81 0.06881825348339987 1103 82-83 0.02731197038426103 1103 84-85 0.005247047759617374 1103 86-87 -0.024799205044715222 1103 88-89 -0.1053838302014114 1103 90-91 -0.005688299939137664 1103 92-93 -0.01526652258164063 1103 94-95 -0.014126344354309595 1103 96-97 -0.015159880776138834 1103 98-99 -0.08374896443860536 1103 100-101 -0.06417820804311702 1104 1 -0.047872563531306866 1104 2 -0.014805452199816216 1104 3 0.03323871505519804 1104 4 -0.028054915570287164 1104 5 -0.03660404889617297 1104 6 0.10298661382930874 1104 7 0.0533025363830717 1104 8 0.1290389360701525 1104 9 0.1899626374663299 1104 10-11 -0.06031349167385969 1104 12-13 -0.04928703683558666 1104 14-15 -0.005350386890995651 1104 16-17 0.12131984544818408 1104 18-19 0.057300229171389105 1104 20-21 0.056678795605691334 1104 22-23 0.02190967346847117 1104 24-25 0.06253565189244625 1104 26-27 0.12729106782482802 1104 28-29 0.09375667404245291 1104 30-31 0.07682997502345046 1104 32-33 0.004925241026455751 1104 34-35 0.06884210796323487 1104 36-37 0.1299034653425224 1104 38-39 0.11737376518347986 1104 40-41 0.08231028818845232 1104 42-43 0.04858018441963452 1104 44-45 0.08725330480165638 1104 46-47 0.11335684546470759 1104 48-49 0.12178511150722215 1104 50-51 0.013983809696419769 1104 52-53 -0.04837106440333372 1104 54-55 -0.03797916967574366 1104 56-57 0.051776170513051056 1104 58-59 0.025085471009603566 1104 60-61 0.01763732921405392 1104 62-63 -0.03563112525538514 1104 64-65 0.015720357255496253 1104 66-67 -0.05699541629656579 1104 68-69 -0.11158113774571632 1104 70-71 -0.04754371931693413 1104 72-73 -0.07101378937270653 1104 74-75 -0.1315607258866791 1104 76-77 -0.09684794534416596 1104 78-79 -0.031135401032408083 1104 80-81 -0.04558998141352788 1104 82-83 -0.08306370782449335 1104 84-85 -0.16772142194620443 1104 86-87 -0.1511704826434368 1104 88-89 -0.1745374170054106 1104 90-91 -0.20854505022213488 1104 92-93 -0.19601097449024962 1104 94-95 -0.16622530505397037 1104 96-97 -0.19225835512534672 1104 98-99 -0.3183465680815587 1104 100-101 -0.2997887157029382 1105 1 -0.35574520580867386 1105 2 -0.17243666439529903 1105 3 -0.29506970002206145 1105 4 -0.3309558335234186 1105 5 -0.4157473164944534 1105 6 -0.27549721599065435 1105 7 -0.23504244654060358 1105 8 -0.14914022133081062 1105 9 -0.1600712647200453 1105 10-11 -0.2789874242022492 1105 12-13 -0.32308476154268106 1105 14-15 -0.3849719526174056 1105 16-17 -0.3593236162602338 1105 18-19 -0.31861374335979065 1105 20-21 -0.28780024676041194 1105 22-23 -0.2905608745661894 1105 24-25 -0.16712929789832742 1105 26-27 -0.15973199818515127 1105 28-29 -0.1589663245209536 1105 30-31 -0.003860091009784128 1105 32-33 -0.05251427622071958 1105 34-35 -0.10209772504837389 1105 36-37 -0.08776807166989897 1105 38-39 -0.15425252001352163 1105 40-41 -0.0795495732330096 1105 42-43 -0.15462692663104605 1105 44-45 -0.16595434295909683 1105 46-47 -0.20466641554992293 1105 48-49 -0.09706933450958388 1105 50-51 -0.10018122149536879 1105 52-53 -0.08357763516587369 1105 54-55 0.010310693123265935 1105 56-57 -0.020197008295490093 1105 58-59 -0.03669057418854038 1105 60-61 -4.0444829814845207E-4 1105 62-63 -0.007657170006268643 1105 64-65 0.0124162805846737 1105 66-67 -0.10763159663830635 1105 68-69 -0.06570888547766884 1105 70-71 -0.1297054443746788 1105 72-73 -0.14048357349002316 1105 74-75 -0.06966231079152863 1105 76-77 -0.09525803119988296 1105 78-79 -0.07782023235338187 1105 80-81 -0.10586896331030005 1105 82-83 -0.15210117197673867 1105 84-85 -0.13252639045025916 1105 86-87 -0.16054000931882229 1105 88-89 -0.2044321681597836 1105 90-91 -0.1690095549013506 1105 92-93 -0.16914765128881015 1105 94-95 -0.15425756490752462 1105 96-97 -0.23719369050652972 1105 98-99 -0.19533369240686227 1105 100-101 -0.2407254715767806 1106 1 0.024985312072129062 1106 2 0.1339195257319119 1106 3 0.06824050157280936 1106 4 0.036829416098441925 1106 5 0.03865635354750907 1106 6 0.16056445720595747 1106 7 0.16406485408266036 1106 8 0.201049404860278 1106 9 0.17075810220192977 1106 10-11 0.18966903861009854 1106 12-13 0.11638099516589762 1106 14-15 0.18534675150645796 1106 16-17 0.21300288719027805 1106 18-19 0.09748258008747968 1106 20-21 0.20112135241112128 1106 22-23 0.17016109864780304 1106 24-25 0.19593515709708242 1106 26-27 0.25506211268698564 1106 28-29 0.20843830035811806 1106 30-31 0.19486726177828473 1106 32-33 0.22767695163176427 1106 34-35 0.1479109085039596 1106 36-37 0.2041454405223817 1106 38-39 0.21507228373659615 1106 40-41 0.2365833946267486 1106 42-43 0.1745903471022956 1106 44-45 0.17272203881960024 1106 46-47 0.17095885465840865 1106 48-49 0.2060091723456594 1106 50-51 0.19144018056358192 1106 52-53 0.28388683841203033 1106 54-55 0.23291126533140272 1106 56-57 0.2266818880213819 1106 58-59 0.2649609823154293 1106 60-61 0.26878289154813473 1106 62-63 0.23098077375352233 1106 64-65 0.3164223362980394 1106 66-67 0.2950906356021221 1106 68-69 0.2483431412691104 1106 70-71 0.2066888845279351 1106 72-73 0.15673435086941367 1106 74-75 0.20531058210607256 1106 76-77 0.2233797199525398 1106 78-79 0.21736481663095475 1106 80-81 0.17971852286989076 1106 82-83 0.1321606019400221 1106 84-85 0.16559527178097966 1106 86-87 0.18124602103846144 1106 88-89 0.18999773410470056 1106 90-91 0.12050453844059916 1106 92-93 0.16861583250845769 1106 94-95 0.22374332641155092 1106 96-97 0.13853431466944244 1106 98-99 0.09321091294892625 1106 100-101 0.17422774569678978 1107 1 0.08162943015999247 1107 2 0.11253328123297024 1107 3 0.13923151148742363 1107 4 0.07947718401894832 1107 5 0.13156409103235234 1107 6 0.16612509529180386 1107 7 0.1176678486450271 1107 8 0.15215315229569626 1107 9 0.16277511626483943 1107 10-11 0.11486669413490347 1107 12-13 0.06304220262465066 1107 14-15 0.08075829096920017 1107 16-17 0.09472643824135929 1107 18-19 0.15948474459232642 1107 20-21 0.13614139210197607 1107 22-23 0.16310982152463538 1107 24-25 0.17120358227514032 1107 26-27 0.1222703622908412 1107 28-29 0.11891653862774376 1107 30-31 0.20181447378378436 1107 32-33 0.21337206404523812 1107 34-35 0.24261564215140652 1107 36-37 0.1774397129089209 1107 38-39 0.15486491960073323 1107 40-41 0.1264760574149264 1107 42-43 0.15398465298475372 1107 44-45 0.2386278697149251 1107 46-47 0.23723741764509043 1107 48-49 0.2219060619693849 1107 50-51 0.25135435716929067 1107 52-53 0.25569011010112064 1107 54-55 0.22240887229869344 1107 56-57 0.25519797255578425 1107 58-59 0.2587770075044986 1107 60-61 0.2594391593908867 1107 62-63 0.14662764153297303 1107 64-65 0.1917369444407413 1107 66-67 0.21216196894918227 1107 68-69 0.18718992740843987 1107 70-71 0.1842932827862569 1107 72-73 0.16268719386104635 1107 74-75 0.17022460040797682 1107 76-77 0.21812026208561264 1107 78-79 0.24064797365188184 1107 80-81 0.22231154578062728 1107 82-83 0.18629389461035828 1107 84-85 0.1633548493057404 1107 86-87 0.20176249360316945 1107 88-89 0.2806726956426644 1107 90-91 0.22963937674298052 1107 92-93 0.22643298487846408 1107 94-95 0.24601712102315076 1107 96-97 0.18250956641175975 1107 98-99 0.14568503813027434 1107 100-101 0.1143796857285011 1108 1 0.15116131224396412 1108 2 0.1524132023122462 1108 3 0.16385986000661745 1108 4 0.11818343850747937 1108 5 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-0.0035130125212603502 2213 100-101 0.08602581163965795 2214 1 0.041426797330593956 2214 2 -0.047540106484206035 2214 3 0.032684270140237004 2214 4 0.047560587221632034 2214 5 0.0469329748277616 2214 6 0.009618779512813092 2214 7 -0.024534439085449833 2214 8 -0.13001289418895112 2214 9 -0.14953786970797012 2214 10-11 -0.16730978880907088 2214 12-13 -0.16976001380101025 2214 14-15 -0.20636141177700296 2214 16-17 -0.2159422011724601 2214 18-19 -0.23242001249962385 2214 20-21 -0.1430332721882479 2214 22-23 -0.17266725453247744 2214 24-25 -0.1652071516489073 2214 26-27 -0.1954315718450701 2214 28-29 -0.16778178749601125 2214 30-31 -0.1382782796075901 2214 32-33 -0.16356347803837679 2214 34-35 -0.17914987572840602 2214 36-37 -0.2798787205294673 2214 38-39 -0.17900744770170007 2214 40-41 -0.13876772802143478 2214 42-43 -0.19780484458285486 2214 44-45 -0.16049617078996192 2214 46-47 -0.27621752204462524 2214 48-49 -0.22774095134299444 2214 50-51 -0.2149120767753132 2214 52-53 -0.11997361209249391 2214 54-55 -0.12978559650969856 2214 56-57 -0.08443927516020722 2214 58-59 -0.21074516231815466 2214 60-61 -0.17186810198257518 2214 62-63 -0.18688798195648104 2214 64-65 -0.11713395077164535 2214 66-67 -0.12965133546381225 2214 68-69 -0.12242543523544924 2214 70-71 -0.1162668218449312 2214 72-73 -0.15657987960048558 2214 74-75 -0.057335767002264504 2214 76-77 -0.07433679243357005 2214 78-79 -0.09036769671009637 2214 80-81 -0.11688539320951463 2214 82-83 -0.06015038538966877 2214 84-85 -0.12131177283202987 2214 86-87 -0.02992049495348681 2214 88-89 -0.029083028224299312 2214 90-91 -0.14016736786096828 2214 92-93 -0.11378324830528896 2214 94-95 -0.014585113214060641 2214 96-97 -0.11976739070632902 2214 98-99 -0.15522097104384613 2214 100-101 -0.2400465375124199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 29.0 12 220.0 13 675.0 14 1659.0 15 2929.0 16 4175.0 17 5845.0 18 8845.0 19 13553.0 20 13903.0 21 13647.0 22 13934.0 23 15102.0 24 17850.0 25 22053.0 26 28396.0 27 37062.0 28 49460.0 29 65992.0 30 87543.0 31 116777.0 32 156075.0 33 211893.0 34 301046.0 35 454974.0 36 756779.0 37 1360826.0 38 1774904.0 39 618998.0 40 1403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70201880166908 21.11774723226182 12.183947687399904 24.996286278669196 2 15.513214219697833 21.275749722974545 38.209127497330456 25.001908559997165 3 18.1345913108139 26.42959554126833 29.452295433691827 25.98351771422594 4 12.468413716840391 17.85073098581689 38.59313066459797 31.087724632744738 5 12.207523794997792 39.2708547032287 35.127632994452476 13.39398850732103 6 29.27326656540864 38.97228788400774 18.09214870213816 13.662296848445452 7 26.890795476491782 33.52402457935444 22.290539716324844 17.294640227828932 8 25.569243418674976 35.772459538614896 20.212524906404546 18.44577213630558 9 25.39755632579226 17.15280752252601 22.27340349276843 35.1762326589133 10-11 23.654385907776586 27.376484281139195 28.87090312335463 20.09822668772959 12-13 24.211867720049007 25.2980200433717 28.821503735290666 21.668608501288627 14-15 21.781147197886348 27.090858856154643 26.661308145196276 24.466685800762733 16-17 20.66304515727886 30.43861910300722 27.42612785182088 21.47220788789304 18-19 20.614332802354046 29.246985608333496 29.72752267544691 20.411158913865542 20-21 22.21594435454018 28.231473508941452 29.130353709521355 20.42222842699701 22-23 21.74854776596434 27.990080156919078 29.402730328305683 20.8586417488109 24-25 21.183482824549923 28.45711128101149 29.207750965678986 21.1516549287596 26-27 20.67471565644974 28.696531124823338 29.286350193915457 21.342403024811464 28-29 21.03319139204254 28.41411144188875 29.58163486739946 20.971062298669253 30-31 21.245368143744166 28.891169387265496 29.042098097489355 20.821364371500984 32-33 20.6826421750237 28.568553584158916 29.59298301694667 21.15582122387071 34-35 21.20683194432465 28.375092929496702 29.333884941060322 21.084190185118317 36-37 21.48616050972712 28.335216693637943 28.93490330378269 21.243719492852247 38-39 21.087779858488904 28.574636537449795 29.355358009820108 20.98222559424119 40-41 20.856254047519155 28.315847076016126 29.565077773278503 21.262821103186216 42-43 20.766600173766236 28.371463744205965 29.509309357989146 21.352626724038654 44-45 20.894514118217852 28.41780354546029 29.35549607418052 21.332186262141338 46-47 21.232243908072526 28.44820206904875 29.111455135011514 21.208098887867212 48-49 21.10382781002799 28.28325576552708 29.220509736867196 21.392406687577733 50-51 21.130782846039235 28.289249383055342 29.022233072456665 21.55773469844876 52-53 21.073616079397727 28.428239586820474 29.059900278548906 21.438244055232893 54-55 21.09417954766542 28.94079946411537 28.974868875404063 20.99015211281515 56-57 21.17930840800585 28.468391951400047 28.882049018045663 21.470250622548445 58-59 21.22517826139287 28.02826713472109 29.187804726316642 21.558749877569397 60-61 21.320182784218886 28.374930500837404 28.938038176907227 21.366848538036486 62-63 20.998850167520796 28.315132389915192 29.13809343513712 21.54792400742689 64-65 20.993879850546143 28.40886996919865 29.005957720794555 21.591292459460647 66-67 20.866462688756314 28.232789181081802 29.112535285595875 21.788212844566008 68-69 21.393771088315873 28.603808724660517 28.810125607706528 21.192294579317082 70-71 21.35260354461566 28.205988452296893 28.8516748587561 21.589733144331348 72-73 20.99538231564469 28.146247191405443 29.141049636736426 21.71732085621344 74-75 20.95886023160053 28.324926838071136 29.097997920588302 21.618215009740034 76-77 21.061628844341204 28.376830916151242 28.86241951456896 21.6991207249386 78-79 21.11329740086532 28.328890097358112 29.02072248592515 21.537090015851412 80-81 21.03905126069816 28.26041017459619 29.005031877436533 21.695506687269116 82-83 20.950836255830986 28.22099686041644 29.2912068108286 21.53696007292397 84-85 20.877142373105453 28.16573863052174 29.016872926699683 21.940246069673126 86-87 21.025585924841987 28.219729916873888 29.005389220487 21.749294937797135 88-89 20.950162176894878 28.19634831136729 29.075590887037528 21.7778986247003 90-91 20.77899485572193 27.972058697169473 29.171326338830404 22.077620108278193 92-93 20.919560617482293 28.33802670944388 28.90561741651045 21.836795256563377 94-95 20.929135786948176 27.88377059940561 29.14303938781288 22.04405422583334 96-97 20.93867034924923 28.155099553337433 28.89164855181044 22.0145815456029 98-99 20.62700223777964 28.07870123343451 29.02383299475079 22.270463534035056 100-101 20.69202243009842 28.136404014651717 28.93405055332135 22.237523001928515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 65.0 1 48.0 2 101.0 3 477.5 4 502.5 5 355.5 6 1242.5 7 3369.0 8 5567.0 9 6229.5 10 4242.5 11 1903.0 12 1443.0 13 1540.5 14 1620.0 15 1730.0 16 1939.0 17 2204.0 18 2776.0 19 3677.5 20 4890.0 21 6946.0 22 11095.5 23 18577.5 24 26084.0 25 33441.5 26 45279.5 27 61583.0 28 84021.5 29 102126.5 30 110757.5 31 123137.0 32 142022.0 33 158891.5 34 173906.5 35 191378.0 36 203685.5 37 212796.5 38 223123.0 39 229389.5 40 234606.5 41 234366.5 42 231307.0 43 232461.0 44 229431.5 45 223749.5 46 220777.0 47 218141.5 48 211448.0 49 206511.5 50 203940.5 51 190790.5 52 169742.5 53 151056.0 54 139038.5 55 134226.0 56 122310.0 57 104100.0 58 91741.0 59 81848.0 60 69414.0 61 55995.0 62 45194.5 63 35833.0 64 28087.0 65 22594.0 66 17964.5 67 13548.5 68 9681.0 69 6941.0 70 4930.0 71 3348.0 72 2385.5 73 1624.0 74 1106.5 75 810.0 76 493.0 77 351.0 78 266.5 79 138.5 80 57.0 81 26.5 82 14.5 83 10.5 84 4.5 85 1.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059952418148543934 2 0.001218214944768571 3 0.0 4 0.0 5 0.001754229520466742 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0012263363777336945 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.1115725709321896E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.248573186049522E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6156549.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.29315332187952 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33107131819418 33.48216057139715 2 17.466550973664063 16.870296483511655 3 6.214733829338461 9.003872810247412 4 2.6940425310439604 5.204152360294815 5 1.3850770783629245 3.3444869854000823 6 0.7480263352133751 2.1674730297157905 7 0.47359451870160696 1.6009960892840909 8 0.2958153630970736 1.1428685348011554 9 0.22288894455579378 0.9687608975866369 >10 0.9694270900715198 8.457553660610209 >50 0.09612116996161009 3.2711536264533416 >100 0.09006700324099833 8.849380663515616 >500 0.009716232959730568 3.201804105555031 >1k 0.0027664017738553435 2.05998749144779 >5k 1.0120982095625976E-4 0.37505269017932286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9541 0.15497318384049247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8426 0.13686238832826636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.248573186049522E-5 0.0 4 0.0 0.0 0.0 8.121432965123805E-5 0.0 5 0.0 0.0 0.0 8.121432965123805E-5 0.0 6 0.0 0.0 0.0 8.121432965123805E-5 0.0 7 0.0 0.0 0.0 8.121432965123805E-5 0.0 8 0.0 0.0 0.0 8.121432965123805E-5 0.0 9 0.0 0.0 0.0 1.461857933722285E-4 0.0 10-11 0.0 0.0 0.0 1.624286593024761E-4 0.0 12-13 0.0 0.0 0.0 2.0303582412809515E-4 0.0 14-15 0.0 0.0 0.0 3.4110018453519983E-4 0.0 16-17 0.0 0.0 0.0 3.5734305046544745E-4 0.0 18-19 0.0 2.4364298895371414E-5 0.0 3.979502152910665E-4 0.0 20-21 0.0 3.248573186049522E-5 0.0 4.3043594715156167E-4 0.0 22-23 0.0 3.248573186049522E-5 0.0 5.360145756981711E-4 0.0 24-25 0.0 3.248573186049522E-5 0.0 8.689933272682472E-4 0.0 26-27 0.0 3.248573186049522E-5 0.0 0.0013075507073849327 0.0 28-29 0.0 3.248573186049522E-5 0.0 0.002639465713665237 0.0 30-31 0.0 4.8728597790742835E-5 0.0 0.007187468174134568 0.0 32-33 0.0 4.8728597790742835E-5 0.0 0.015520058396351594 0.0 34-35 0.0 4.8728597790742835E-5 0.0 0.027685964978107054 0.0 36-37 0.0 4.8728597790742835E-5 0.0 0.04904533367638266 0.0 38-39 0.0 4.8728597790742835E-5 0.0 0.08134427257868004 0.0 40-41 0.0 4.8728597790742835E-5 0.0 0.11788259948877204 0.0 42-43 0.0 4.8728597790742835E-5 0.0 0.15377933319461926 0.0 44-45 0.0 5.685003075586664E-5 0.0 0.19846345736873044 0.0 46-47 0.0 9.745719558148567E-5 0.0 0.25494802364116653 0.0 48-49 0.0 9.745719558148567E-5 0.0 0.31784040052308526 0.0 50-51 0.0 9.745719558148567E-5 0.0 0.38446863656896096 0.0 52-53 0.0 9.745719558148567E-5 0.0 0.4520308374058259 0.0 54-55 0.0 9.745719558148567E-5 0.0 0.5281530285879313 0.0 56-57 0.0 1.2182149447685707E-4 0.0 0.6220449150977276 0.0 58-59 0.0 1.2994292744198088E-4 0.0 0.7285412655693961 0.0 60-61 0.0 1.2994292744198088E-4 0.0 0.8447670927332829 0.0 62-63 0.0 1.2994292744198088E-4 0.0 0.9634455926526371 0.0 64-65 0.0 1.2994292744198088E-4 0.0 1.089384653642812 0.0 66-67 0.0 1.624286593024761E-4 0.0 1.2385672557791712 0.0 68-69 0.0 1.7867152523272373E-4 0.0 1.4118380280900875 0.0 70-71 0.0 1.7867152523272373E-4 0.0 1.6032764459439859 0.0 72-73 0.0 1.7867152523272373E-4 0.0 1.8038433544506833 0.0 74-75 0.0 1.7867152523272373E-4 0.0 2.010371394753782 0.0 76-77 0.0 1.7867152523272373E-4 0.0 2.233459036872768 0.0 78-79 0.0 1.7867152523272373E-4 0.0 2.493791570569811 0.0 80-81 0.0 1.8679295819784753E-4 0.0 2.782337962387695 0.0 82-83 0.0 1.9491439116297134E-4 0.0 3.0859983409536738 0.0 84-85 0.0 1.9491439116297134E-4 0.0 3.3911855489170963 0.0 86-87 0.0 1.9491439116297134E-4 0.0 3.718641726070888 0.0 88-89 0.0 1.9491439116297134E-4 0.0 4.086964953905182 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9580 0.0 52.1462 1 GTATCAA 16770 0.0 38.830494 1 ATCAACG 20210 0.0 31.658499 3 TCAACGC 20435 0.0 31.216948 4 CAACGCA 21350 0.0 29.924545 5 TATCAAC 22150 0.0 29.036755 2 AACGCAG 23220 0.0 27.596428 6 GTGGTAT 4660 0.0 26.41796 1 TGGTATC 4665 0.0 25.557903 2 CGCAGAG 26030 0.0 24.015125 8 ACGCAGA 26920 0.0 23.53878 7 GTACATG 31670 0.0 23.233162 1 TACATGG 30385 0.0 22.574133 2 ACATGGG 30935 0.0 21.388979 3 CATGGGT 6960 0.0 21.019894 4 GCAGAGT 29295 0.0 20.900785 9 TCTGTCG 1085 0.0 20.576488 8 TACGACG 120 0.0047652097 19.7921 5 CTGTCGC 1210 0.0 18.843391 9 TACCTGG 4390 0.0 18.827293 2 >>END_MODULE