FastQCFastQC Report
Wed 25 May 2016
SRR2049424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620910
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106990.2315344813034662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.041.2108351
TACCTGG46200.038.636652
GTACCTG49150.037.9799161
GTATCAA76000.033.2088431
ATCAACG77800.030.693193
TCAACGC78050.030.5948774
CAACGCA86250.027.9035935
CCCGAGG18150.027.4641593
ACCTGGG65900.026.7265033
TATCAAC91300.026.5707442
CCGAGGG18600.025.778764
GTACATG162450.025.3857331
AACGCAG97750.025.2354496
TACATGG156950.025.0753422
ACATGGG159400.024.1836223
TCTGTCG10250.022.2205418
CGCAGAG104450.021.9874178
ACGCAGA110150.021.8366267
CGAGGGC22400.021.1915765
CATGGGG95300.020.1252864
Produced by FastQC (version 0.11.5)