##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049424_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4620910 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.695103561852534 31.0 31.0 33.0 28.0 34.0 2 30.807461084505 31.0 31.0 33.0 28.0 34.0 3 30.937151340320412 31.0 31.0 34.0 28.0 34.0 4 34.704552999301 35.0 35.0 37.0 32.0 37.0 5 34.791547552321944 37.0 35.0 37.0 32.0 37.0 6 35.19946265995226 37.0 35.0 37.0 32.0 37.0 7 35.21072407815777 37.0 35.0 37.0 32.0 37.0 8 35.05256367252338 37.0 35.0 37.0 32.0 37.0 9 36.899882490678245 39.0 37.0 39.0 33.0 39.0 10-11 36.84132930526671 39.0 37.0 39.0 32.5 39.0 12-13 36.86624290453612 39.0 37.0 39.0 32.5 39.0 14-15 37.98093600611135 40.0 38.0 41.0 32.5 41.0 16-17 37.9394839977407 40.0 38.0 41.0 32.0 41.0 18-19 37.952636060862474 40.0 37.5 41.0 32.0 41.0 20-21 38.05085480998331 40.0 38.0 41.0 32.0 41.0 22-23 37.87891335256475 40.0 37.0 41.0 32.0 41.0 24-25 37.88224126849474 40.0 37.5 41.0 32.0 41.0 26-27 37.88103295671199 40.0 37.0 41.0 32.0 41.0 28-29 37.54439158087909 40.0 37.0 41.0 31.0 41.0 30-31 37.58206900805253 40.0 37.0 41.0 31.0 41.0 32-33 37.56811472199199 40.0 37.0 41.0 31.0 41.0 34-35 37.65720583175175 40.0 37.0 41.0 31.0 41.0 36-37 37.66782852295327 40.0 37.0 41.0 31.0 41.0 38-39 37.44650599124415 40.0 37.0 41.0 30.5 41.0 40-41 37.58064753479293 40.0 37.0 41.0 31.0 41.0 42-43 37.22589608973124 40.0 36.0 41.0 30.0 41.0 44-45 37.17259901188294 39.0 36.0 41.0 30.0 41.0 46-47 37.1013003932126 39.0 36.0 41.0 30.0 41.0 48-49 36.960628858817856 39.0 35.0 41.0 30.0 41.0 50-51 36.84640795860555 39.0 35.0 40.0 30.0 41.0 52-53 36.69874829849532 39.0 35.0 40.0 30.0 41.0 54-55 36.51770268193927 39.0 35.0 40.0 30.0 41.0 56-57 36.29442155333041 38.0 35.0 40.0 29.5 41.0 58-59 36.02516030392282 38.0 34.0 40.0 29.0 41.0 60-61 35.75844368317063 38.0 34.0 40.0 28.5 41.0 62-63 35.46691993568366 38.0 34.0 40.0 28.0 41.0 64-65 35.16301529352444 37.0 34.0 40.0 28.0 41.0 66-67 34.87837936683467 37.0 33.0 39.5 28.0 41.0 68-69 34.54196392052648 36.0 33.0 39.0 28.0 41.0 70-71 34.18908851286868 36.0 33.0 39.0 27.0 40.0 72-73 33.81129820749592 35.0 32.5 38.5 26.0 40.0 74-75 33.43084305905114 35.0 32.0 38.0 26.0 40.0 76-77 33.077193020422385 35.0 32.0 37.0 26.0 39.0 78-79 32.869526024094824 35.0 32.0 37.0 26.0 39.0 80-81 32.75131002335038 35.0 32.0 36.5 26.0 39.0 82-83 32.448220588585365 35.0 32.0 36.0 26.0 38.5 84-85 32.12570911357287 35.0 32.0 36.0 26.0 37.0 86-87 31.69417116974795 34.5 31.5 35.0 24.5 37.0 88-89 31.281006663189718 34.0 31.0 35.0 24.0 36.5 90-91 31.082029513667223 34.0 31.0 35.0 24.0 36.0 92-93 30.859374885033468 34.0 31.0 35.0 23.0 36.0 94-95 30.59044106031063 34.0 31.0 35.0 20.5 36.0 96-97 30.313145138078863 34.0 31.0 35.0 19.5 35.0 98-99 30.00435011718471 34.0 31.0 35.0 14.5 35.0 100-101 29.435470177951963 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1947621280669729 1101 2 0.3108386624446595 1101 3 0.24525824203808 1101 4 0.31907775744112143 1101 5 0.25403720797079643 1101 6 0.20456869296467062 1101 7 0.26986143412954533 1101 8 0.377493485589099 1101 9 0.36072519943736125 1101 10-11 0.3516923431585326 1101 12-13 0.35331023000091477 1101 14-15 0.5571873202245072 1101 16-17 0.5292773567174791 1101 18-19 0.46860831569352257 1101 20-21 0.5854394987195803 1101 22-23 0.5394537763713174 1101 24-25 0.16132599680276627 1101 26-27 0.4145074522705059 1101 28-29 0.8771671425106007 1101 30-31 0.5148911096940978 1101 32-33 0.8050649970671628 1101 34-35 0.6429081641169105 1101 36-37 0.6213309482753004 1101 38-39 0.646300397556189 1101 40-41 0.5151423319295674 1101 42-43 0.6364312169800073 1101 44-45 0.4114259349354583 1101 46-47 0.2501526945804855 1101 48-49 0.168053797350602 1101 50-51 0.3106305977435113 1101 52-53 0.18768623542274554 1101 54-55 0.19373150461126443 1101 56-57 0.03226340310845188 1101 58-59 0.013642133334322182 1101 60-61 0.04804972280292219 1101 62-63 -0.0012215503855586007 1101 64-65 0.014054310319366436 1101 66-67 -0.04510013070570551 1101 68-69 -0.18052177051445994 1101 70-71 -0.14291404347762438 1101 72-73 -0.1859949440545563 1101 74-75 -0.22860326501604789 1101 76-77 -0.29922043530969233 1101 78-79 -0.24757124985759305 1101 80-81 -0.3179829934164431 1101 82-83 -0.41770259119438435 1101 84-85 -0.3875350585928814 1101 86-87 -0.45118952139025126 1101 88-89 -0.41700921549450953 1101 90-91 -0.5560412954963958 1101 92-93 -0.6622509798041065 1101 94-95 -0.6345345865029515 1101 96-97 -0.7438575146708288 1101 98-99 -0.796279897402183 1101 100-101 -0.9883086866552482 1102 1 -0.09075207516390904 1102 2 -0.15431133018655174 1102 3 -0.17835975082677535 1102 4 -0.0772908512552064 1102 5 -0.01397396277954499 1102 6 -0.011096422386643212 1102 7 -0.006681223166616235 1102 8 0.05189759004229444 1102 9 0.22644258696107755 1102 10-11 0.2933215945670966 1102 12-13 0.3774218206025637 1102 14-15 0.49723292556349463 1102 16-17 0.5280969943696618 1102 18-19 0.5150972754431535 1102 20-21 0.473292131032089 1102 22-23 -0.048304739497766036 1102 24-25 0.46093490855555785 1102 26-27 0.6281775413308281 1102 28-29 0.6189975987641603 1102 30-31 0.5176529720513372 1102 32-33 0.8119156795474609 1102 34-35 0.25421690027126687 1102 36-37 0.4448987877144148 1102 38-39 0.5155454007185085 1102 40-41 0.39189092710883955 1102 42-43 0.33169030477411354 1102 44-45 0.1530786778988258 1102 46-47 0.04592572866354061 1102 48-49 0.007236965496481673 1102 50-51 0.10703101300224205 1102 52-53 0.08237434245293684 1102 54-55 0.04418504906176679 1102 56-57 -0.03069404180637747 1102 58-59 -0.10118269801754565 1102 60-61 -0.11189118484551841 1102 62-63 -0.1626440784529919 1102 64-65 -0.1526347761726683 1102 66-67 -0.10903270633413342 1102 68-69 -0.224004139011015 1102 70-71 -0.31154715806760436 1102 72-73 -0.38368829812377214 1102 74-75 -0.36438443227689277 1102 76-77 -0.4489256700459521 1102 78-79 -0.4336042202089132 1102 80-81 -0.4363636513430791 1102 82-83 -0.48174188454125755 1102 84-85 -0.48215705526461505 1102 86-87 -0.46097040513704357 1102 88-89 -0.5953597294700614 1102 90-91 -0.5992686047439335 1102 92-93 -0.5773526158472109 1102 94-95 -0.5527432981883145 1102 96-97 -0.6573771922280223 1102 98-99 -0.7201078724980512 1102 100-101 -0.9764592057735442 1103 1 0.09711685482992038 1103 2 0.012316326991125948 1103 3 -0.003581860534492165 1103 4 0.04706243254481279 1103 5 -0.12856844877980222 1103 6 0.029498418491783696 1103 7 0.02593589699154819 1103 8 0.13248918712466207 1103 9 0.22880309766971152 1103 10-11 0.25539189813808605 1103 12-13 0.29240933926044477 1103 14-15 0.3923649401649598 1103 16-17 0.46229381896858257 1103 18-19 0.4514776604241888 1103 20-21 0.43482874882102607 1103 22-23 0.06345547187136447 1103 24-25 0.3755389468626973 1103 26-27 0.530370384224824 1103 28-29 0.5061901722541648 1103 30-31 0.5515242283818509 1103 32-33 0.6504478321119151 1103 34-35 0.5803785026251873 1103 36-37 0.4246354631065401 1103 38-39 0.5859618359414682 1103 40-41 0.3970059078708914 1103 42-43 0.1399777931602202 1103 44-45 0.26807367261514514 1103 46-47 0.1857062422234037 1103 48-49 0.09540598963703673 1103 50-51 0.08295173143581991 1103 52-53 0.025488067612535303 1103 54-55 0.11626817219612207 1103 56-57 0.03002936303462178 1103 58-59 -0.025577948624636804 1103 60-61 -0.10884905076553508 1103 62-63 -0.052134777446475766 1103 64-65 -0.09320812729318817 1103 66-67 -0.2648959537415436 1103 68-69 -0.2347228986860017 1103 70-71 -0.24061314656088229 1103 72-73 -0.29564627313481395 1103 74-75 -0.2551472733261164 1103 76-77 -0.2564666669595681 1103 78-79 -0.16164654392740374 1103 80-81 -0.31343768082204804 1103 82-83 -0.3285734199320629 1103 84-85 -0.33498691542400394 1103 86-87 -0.3054514982556533 1103 88-89 -0.2835980049029132 1103 90-91 -0.32147438293944575 1103 92-93 -0.3663801628546892 1103 94-95 -0.388337402800115 1103 96-97 -0.37510333732596024 1103 98-99 -0.5240061873115884 1103 100-101 -0.48634508092633766 1104 1 0.15968199748565937 1104 2 0.24603890896171166 1104 3 0.08531991989007182 1104 4 0.2681810297167644 1104 5 0.039376935366320254 1104 6 0.08123146443828944 1104 7 0.15739974474166019 1104 8 0.18522579907202186 1104 9 0.3357804925791754 1104 10-11 0.29991285891647124 1104 12-13 0.3424268637735608 1104 14-15 0.5176017350928888 1104 16-17 0.5699673646578063 1104 18-19 0.4490268594247482 1104 20-21 0.465046911143169 1104 22-23 0.6571579597475221 1104 24-25 0.7367047710857477 1104 26-27 0.6802503631027434 1104 28-29 0.6059577987023843 1104 30-31 0.7558829435032379 1104 32-33 0.7766515183930522 1104 34-35 0.6317793956332238 1104 36-37 0.4424015419945775 1104 38-39 0.5599714084171694 1104 40-41 0.47660393095817 1104 42-43 0.5031532231567226 1104 44-45 0.3259047033250937 1104 46-47 0.19422549979031345 1104 48-49 0.201661514393912 1104 50-51 0.14945360694746057 1104 52-53 0.1376407893268805 1104 54-55 0.19545629523320684 1104 56-57 0.21768401303439333 1104 58-59 0.17405492008985846 1104 60-61 0.13927312088878097 1104 62-63 0.15894627493843672 1104 64-65 0.1400322066204538 1104 66-67 0.1513618272522308 1104 68-69 0.12210434220564537 1104 70-71 -0.005987127445457929 1104 72-73 -0.03490721772923422 1104 74-75 0.05180293758059662 1104 76-77 -0.05361443955979439 1104 78-79 -0.1487807319868324 1104 80-81 -0.1516890163446547 1104 82-83 -0.1397493237932892 1104 84-85 -0.22702397770776628 1104 86-87 -0.14638893758934657 1104 88-89 -0.09041681141092539 1104 90-91 -0.04496571986935649 1104 92-93 -0.12450239969092536 1104 94-95 -0.04522359406491816 1104 96-97 -0.04636852314312634 1104 98-99 -0.04821129769992183 1104 100-101 -0.10695068066598878 1105 1 0.06456196090652355 1105 2 0.1318563266144821 1105 3 0.02929550382713586 1105 4 0.11170853297868888 1105 5 -0.09286335610453023 1105 6 0.04549350412170128 1105 7 0.07038770378213144 1105 8 -0.10651521722799373 1105 9 -0.08301235764465531 1105 10-11 0.22374804008646265 1105 12-13 0.20708446493847532 1105 14-15 0.3071023112064353 1105 16-17 0.05384330225339795 1105 18-19 0.2100301225302772 1105 20-21 0.10649349749620285 1105 22-23 0.43185151475581307 1105 24-25 0.10057739423699985 1105 26-27 -0.006015837084390796 1105 28-29 0.46764179900158354 1105 30-31 0.39904021356727526 1105 32-33 0.4338104997175094 1105 34-35 -0.19092949043469787 1105 36-37 -0.24106662029648618 1105 38-39 -0.08144235497361763 1105 40-41 -0.06502180619034448 1105 42-43 0.21383866427966325 1105 44-45 -0.046013926060638255 1105 46-47 -0.2272279981582912 1105 48-49 -0.1343403155445131 1105 50-51 -0.23593615511538957 1105 52-53 -0.09281890981797147 1105 54-55 -0.2624114510271127 1105 56-57 -0.4654247163471368 1105 58-59 -0.10954055357579051 1105 60-61 -0.14371232421954971 1105 62-63 -0.20796796316441402 1105 64-65 -0.31721486049142555 1105 66-67 -0.346183714577208 1105 68-69 -0.36517387027767256 1105 70-71 -0.1568408238582677 1105 72-73 -0.14137689476012127 1105 74-75 0.02797360196544929 1105 76-77 -0.005373421492862462 1105 78-79 0.03843290453921355 1105 80-81 -0.07412471229648787 1105 82-83 -0.12353328347300163 1105 84-85 -0.16305417159367863 1105 86-87 -0.06925032588849689 1105 88-89 -0.177910509402178 1105 90-91 -0.060325467388505416 1105 92-93 7.791906494531986E-4 1105 94-95 0.09747841231012089 1105 96-97 -0.07315377817030466 1105 98-99 0.025254222066593 1105 100-101 -0.17742095481710862 1106 1 0.26066929061742883 1106 2 0.2592418884154988 1106 3 0.25107759197145896 1106 4 0.3469854047029415 1106 5 0.2934832552114699 1106 6 0.11804298139677627 1106 7 0.17055399898624302 1106 8 0.13228953164566093 1106 9 0.24998715455328124 1106 10-11 0.06896752966126485 1106 12-13 0.20785844608365522 1106 14-15 0.4359809426869674 1106 16-17 0.37447716860907576 1106 18-19 0.4099735285285604 1106 20-21 0.3867078013296563 1106 22-23 0.5079496989656604 1106 24-25 0.4130346304542627 1106 26-27 0.43951358569343313 1106 28-29 0.5765661145774246 1106 30-31 0.6237297079827613 1106 32-33 0.6206461477164993 1106 34-35 0.5545787501434702 1106 36-37 0.26759729845665703 1106 38-39 0.46293273127744783 1106 40-41 0.3250304531155308 1106 42-43 -0.5370773970065343 1106 44-45 0.10610904216300554 1106 46-47 0.1590464382431449 1106 48-49 0.10508479918325975 1106 50-51 0.13314308925328078 1106 52-53 0.07982918044545784 1106 54-55 0.053820772443138765 1106 56-57 -0.011490247622070626 1106 58-59 -0.03931150272108397 1106 60-61 0.03972731025727683 1106 62-63 -0.06836375429215735 1106 64-65 0.13493668363047817 1106 66-67 0.09929900747174258 1106 68-69 -0.0020137390796577392 1106 70-71 0.07650158511562921 1106 72-73 0.06747812356227456 1106 74-75 0.11975579770115274 1106 76-77 0.04479672109513899 1106 78-79 0.09721032286737596 1106 80-81 0.09145857992378836 1106 82-83 0.1057642845859661 1106 84-85 0.1293488707732635 1106 86-87 0.20634520350977326 1106 88-89 0.11168503964727705 1106 90-91 0.09348995298678986 1106 92-93 0.12480856641147398 1106 94-95 0.2839538392197838 1106 96-97 0.3588562503736199 1106 98-99 0.2755986486940216 1106 100-101 0.3061424190871982 1107 1 0.18343678954289544 1107 2 -0.023004669107692166 1107 3 0.12283298565890632 1107 4 0.20091431968883455 1107 5 0.06299846805944043 1107 6 0.002539935188565323 1107 7 0.11990175338840459 1107 8 -0.38199528597981924 1107 9 -0.10740906628373637 1107 10-11 -0.3648671177449643 1107 12-13 -0.1696793171194173 1107 14-15 0.0472480697908324 1107 16-17 0.2707671121422237 1107 18-19 0.18322123903985243 1107 20-21 -0.3145807527050124 1107 22-23 0.11936920243030613 1107 24-25 0.2802849926107811 1107 26-27 0.021659189670444334 1107 28-29 -0.34612100720495675 1107 30-31 -0.021074836655159856 1107 32-33 -0.3218752348686067 1107 34-35 -0.2197464106949596 1107 36-37 -0.8019553467072669 1107 38-39 -0.3200483911007552 1107 40-41 -0.42095681625841763 1107 42-43 -0.39090899706748417 1107 44-45 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44-45 0.05383022499002266 2214 46-47 0.07194208020104043 2214 48-49 0.04676598502101115 2214 50-51 0.1590064754163265 2214 52-53 0.0856291447747708 2214 54-55 0.11324252272866175 2214 56-57 0.11263948569860105 2214 58-59 0.028976876815072217 2214 60-61 0.04166039171408187 2214 62-63 0.1557466383145254 2214 64-65 0.13147779655603387 2214 66-67 0.26677248353147576 2214 68-69 0.3283791866696646 2214 70-71 0.3682785500307304 2214 72-73 0.40550096098574073 2214 74-75 0.36587639736146826 2214 76-77 0.358813298148732 2214 78-79 0.3168557267479599 2214 80-81 0.2769889278275599 2214 82-83 0.3366734863151777 2214 84-85 0.22752717023035274 2214 86-87 0.186089659008136 2214 88-89 0.27187479790471514 2214 90-91 0.18373727523057326 2214 92-93 0.06515094777370223 2214 94-95 0.0047679409237950665 2214 96-97 0.09710873832248623 2214 98-99 0.2589004129205712 2214 100-101 0.3013794410099493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 66.0 11 1034.0 12 4353.0 13 8779.0 14 12322.0 15 14214.0 16 15400.0 17 16188.0 18 16962.0 19 17498.0 20 18874.0 21 20350.0 22 23669.0 23 29911.0 24 38153.0 25 43892.0 26 50944.0 27 57428.0 28 69368.0 29 83045.0 30 102176.0 31 126810.0 32 164104.0 33 213155.0 34 292591.0 35 424926.0 36 645185.0 37 930895.0 38 939115.0 39 239375.0 40 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7302984849812 20.47597798731134 13.392950488404084 23.400773039303374 2 15.447285991729165 20.942912359301395 40.042536585534876 23.56726506343456 3 17.78885457377664 26.2665015903073 30.22263869088701 25.722005145029055 4 12.33452446476523 17.84253801320837 38.967391504475465 30.85554601755094 5 12.440771893986941 37.910579258348676 35.07984505290734 14.568803794757043 6 29.162534178910843 38.412497119070835 19.052047163921355 13.372921538096975 7 26.46996699212849 32.89689303562478 23.07906393727725 17.554076034969473 8 25.00696295190207 35.846921098447595 21.12792246506892 18.018193484581417 9 24.61913299892127 17.786339607744 22.347415117110597 35.24711227622414 10-11 23.20549291095931 27.33665666493178 29.314825254578956 20.143025169529945 12-13 23.798049557514464 24.730894342374942 29.31961761631342 22.151438483797172 14-15 21.24046127405499 27.11362627521179 26.65816759935652 24.9877448513767 16-17 20.928797984122497 30.27889739708172 27.018853614688098 21.773451004107685 18-19 21.035807321749285 29.16416469439651 28.875376946087144 20.92465103776706 20-21 22.202908587257618 28.71560119459834 28.10080505540166 20.980685162742382 22-23 21.946963179395613 28.707702296891487 28.127208289370454 21.21812623434245 24-25 21.325751540489147 28.417132248265542 28.374744983668563 21.882371227576748 26-27 21.27253108401758 28.63577449968977 28.268455800672744 21.823238615619907 28-29 21.35199247043914 28.37377543461291 28.672213708639845 21.60201838630811 30-31 21.734860547314796 28.99907105483698 27.985617310300686 21.280451087547537 32-33 21.08089502499508 28.729046285071913 28.61308175548029 21.57697693445272 34-35 21.37252761176854 28.421291026758183 28.37161359462804 21.834567766845232 36-37 21.983284998373687 28.573578749843914 27.89232308398467 21.550813167797727 38-39 21.49431305276073 28.868630451722126 28.316894793453095 21.320161702064045 40-41 21.182563939191812 28.276960681406287 28.727014159749398 21.813461219652503 42-43 21.114968650697723 28.603189532616106 28.37980235363779 21.902039463048382 44-45 21.29360245698826 28.74363955832474 28.340259240786647 21.622498743900355 46-47 21.4788591413802 29.092554898708055 27.980653839203303 21.447932120708444 48-49 21.271839976417468 28.649659799899098 28.585498257823133 21.4930019658603 50-51 21.32828279767527 28.539137394713173 28.337675824351834 21.79490398325973 52-53 21.32349774295948 28.437963904233175 28.36584941967535 21.872688933131997 54-55 21.539430966847323 29.036769180041972 28.13190775473535 21.291892098375357 56-57 21.335489235566587 28.592776010530475 28.142099091568994 21.92963566233394 58-59 21.213055180093654 28.283377360675377 28.47738264395642 22.026184815274547 60-61 21.54622737451214 28.544307585315575 28.072547447385993 21.836917592786296 62-63 21.51473616958282 28.52719681209782 28.2134948234681 21.74457219485126 64-65 21.517432520106123 28.507247294338033 28.237571216226232 21.73774896932961 66-67 21.33447394635331 28.456011458074627 28.15230971431107 22.057204881260994 68-69 21.688882665525973 28.810323981949026 27.95878613979647 21.542007212728528 70-71 21.734705813165732 28.198202009724767 28.136222493478787 21.93086968363071 72-73 21.105116259339464 28.389239913203184 28.58588759613249 21.919756231324854 74-75 21.250117421243353 28.35945965016478 28.48026825288878 21.91015467570309 76-77 21.435989949157 28.672231082803428 28.130487355055898 21.761291612983666 78-79 21.370624524018062 28.35597502150678 28.58614546646582 21.687254988009343 80-81 21.36423465321791 28.305299031292368 28.434714759919586 21.89575155557013 82-83 21.184934740805407 28.25872373670778 28.827683917073927 21.72865760541289 84-85 21.125352204300196 28.17178704871008 28.514111606291902 22.188749140697816 86-87 21.38533183296872 28.29885212985936 28.50216796750508 21.81364806966684 88-89 21.176356973587055 28.229967422312523 28.574111571557136 22.019564032543283 90-91 21.11415732308912 28.0873651127647 28.638328730266682 22.1601488338795 92-93 21.40235451408887 28.246210360253936 28.230009874892627 22.12142525076457 94-95 21.356107421537345 27.95150780935991 28.60638840346959 22.085996365633157 96-97 21.26849708333542 28.150837051056488 28.441327829702406 22.139338035905684 98-99 21.268971217465353 28.01163416313913 28.490896711528624 22.22849790786689 100-101 21.29067241104905 28.11385738630166 28.11913714952442 22.47633305312487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9504.0 1 5637.5 2 1319.5 3 673.0 4 388.0 5 244.0 6 185.5 7 191.5 8 235.0 9 236.0 10 170.0 11 119.5 12 111.5 13 146.0 14 194.5 15 281.0 16 468.0 17 847.5 18 1427.5 19 2239.5 20 3549.0 21 5471.5 22 9141.0 23 15848.0 24 22560.5 25 28458.5 26 37870.5 27 50776.5 28 67441.0 29 81738.5 30 89401.5 31 99923.5 32 113558.5 33 124484.5 34 134177.0 35 143269.5 36 148224.5 37 151551.0 38 156618.5 39 159690.0 40 163139.5 41 163278.5 42 157909.0 43 154797.5 44 152407.5 45 149810.5 46 147867.5 47 146125.0 48 143926.0 49 141859.5 50 141940.0 51 136746.0 52 124647.0 53 116992.0 54 113962.0 55 112433.0 56 107943.0 57 98221.5 58 88564.0 59 79574.0 60 68642.0 61 56154.5 62 44591.5 63 34630.5 64 26754.0 65 21297.0 66 16873.5 67 12820.0 68 9512.0 69 6872.5 70 4971.0 71 3480.5 72 2584.0 73 1896.0 74 1381.5 75 996.0 76 657.0 77 411.0 78 266.0 79 163.0 80 78.0 81 37.5 82 24.0 83 16.5 84 9.0 85 5.0 86 3.0 87 2.0 88 2.0 89 1.5 90 1.5 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 1.5 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4351740241640716 2 0.07007277787275666 3 0.07031082622254058 4 0.0928604971747989 5 0.07773360658398454 6 1.0820379535632592E-4 7 0.004198307259825446 8 5.410189767816296E-4 9 0.01432618250517755 10-11 9.738341582069333E-4 12-13 0.009089118809931377 14-15 0.008721225905719868 16-17 0.017052918148156966 18-19 0.008407434899186524 20-21 0.00238048349783917 22-23 0.011577806103126873 24-25 0.03458193299588176 26-27 0.002748376402050678 28-29 0.00409010346446912 30-31 0.0016771588280230518 32-33 0.002174896286662151 34-35 0.0021532555275908857 36-37 6.708635312092207E-4 38-39 0.11652466721922738 40-41 0.007530984156800283 42-43 0.0050422968636047874 44-45 0.009879006516032556 46-47 0.007682469470299141 48-49 0.011707650657554464 50-51 0.014585871614032733 52-53 0.010365923595136023 54-55 0.009164861466680805 56-57 0.009002555773646316 58-59 0.014607512373103999 60-61 0.018859921530607608 62-63 0.009565215509499212 64-65 0.015960059815058072 66-67 0.012713945954368297 68-69 0.007108989354910613 70-71 6.167616335310577E-4 72-73 0.010041312209067045 74-75 0.017139481184442026 76-77 0.025925629367375688 78-79 0.018513669385467363 80-81 0.028165447931251638 82-83 0.05947962630737236 84-85 0.03483080172520131 86-87 0.35169479604666615 88-89 0.6168265558082715 90-91 0.6089384991267954 92-93 0.6157877993728509 94-95 0.6078456407936965 96-97 0.6020675581216687 98-99 0.6108969878227449 100-101 0.6037988188473699 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4620910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.30726218516219 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63433841899564 43.91704124163831 2 14.064860861040888 16.120373379229925 3 4.202960393882557 7.225804597382407 4 1.735537738427225 3.978356648331697 5 0.9215373572422987 2.6405391472452937 6 0.5233423905714143 1.7994791753451356 7 0.35673702228960213 1.4310535447252994 8 0.25299014968938355 1.1598538270810346 9 0.18009535556197018 0.928869458356785 >10 1.0043954272208273 10.533585725490294 >50 0.07553784768230432 2.994934293834486 >100 0.04359795074982415 4.761353102022367 >500 0.0028521641548990596 1.1214174424427328 >1k 0.0011788936634681902 1.1541721132545426 >5k 0.0 0.0 >10k+ 3.802882785373837E-5 0.23316630361963436 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10699 0.2315344813034662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 2.1640759071265184E-5 0.0 0.0 2.1640759071265184E-5 0.0 10-11 2.1640759071265184E-5 0.0 0.0 2.1640759071265184E-5 0.0 12-13 2.1640759071265184E-5 0.0 0.0 3.2461138606897776E-5 0.0 14-15 2.1640759071265184E-5 0.0 0.0 6.492227721379555E-5 0.0 16-17 2.1640759071265184E-5 0.0 0.0 8.656303628506074E-5 0.0 18-19 2.1640759071265184E-5 0.0 0.0 8.656303628506074E-5 0.0 20-21 2.1640759071265184E-5 1.0820379535632592E-5 0.0 8.656303628506074E-5 0.0 22-23 2.1640759071265184E-5 2.1640759071265184E-5 0.0 2.0558721117701925E-4 0.0 24-25 2.1640759071265184E-5 2.1640759071265184E-5 0.0 4.5445594049656885E-4 0.0 26-27 2.1640759071265184E-5 3.2461138606897776E-5 0.0 8.331692242437096E-4 0.0 28-29 2.1640759071265184E-5 4.328151814253037E-5 0.0 0.0019476683164138665 0.0 30-31 2.1640759071265184E-5 4.328151814253037E-5 0.0 0.005377728629209398 0.0 32-33 2.1640759071265184E-5 5.410189767816296E-5 0.0 0.012281130772942992 0.0 34-35 2.1640759071265184E-5 6.492227721379555E-5 0.0 0.023242175242538805 0.0 36-37 2.1640759071265184E-5 6.492227721379555E-5 0.0 0.04008950617951876 0.0 38-39 2.1640759071265184E-5 6.492227721379555E-5 0.0 0.0662315431376071 0.0 40-41 4.328151814253037E-5 6.492227721379555E-5 0.0 0.099601593625498 0.0 42-43 4.328151814253037E-5 6.492227721379555E-5 0.0 0.13557935558147638 0.0 44-45 4.328151814253037E-5 6.492227721379555E-5 0.0 0.1756255802428526 0.0 46-47 4.328151814253037E-5 6.492227721379555E-5 0.0 0.22683843658500166 0.0 48-49 4.328151814253037E-5 6.492227721379555E-5 0.0 0.28364542914707275 0.0 50-51 4.328151814253037E-5 8.656303628506074E-5 0.0 0.3447048308666475 0.0 52-53 1.298445544275911E-4 9.738341582069332E-5 0.0 0.4065324795332521 0.0 54-55 1.298445544275911E-4 1.1902417489195852E-4 0.0 0.47731940245536053 0.0 56-57 1.298445544275911E-4 1.5148531349885627E-4 0.0 0.5598356167940948 0.0 58-59 1.298445544275911E-4 1.7312607257012147E-4 0.0 0.6533669775001028 0.0 60-61 1.298445544275911E-4 1.7312607257012147E-4 0.0 0.754937880201086 0.0 62-63 1.298445544275911E-4 1.7312607257012147E-4 0.0 0.858954188677122 0.0 64-65 1.298445544275911E-4 1.7312607257012147E-4 0.0 0.9726871979761562 0.0 66-67 1.298445544275911E-4 1.7312607257012147E-4 0.0 1.110019455042405 0.0 68-69 1.298445544275911E-4 1.7312607257012147E-4 0.0 1.260779803112374 0.0 70-71 1.298445544275911E-4 1.7312607257012147E-4 0.0 1.4258987948261272 0.0 72-73 1.298445544275911E-4 1.7312607257012147E-4 0.0 1.6171922846365758 0.0 74-75 1.298445544275911E-4 1.7312607257012147E-4 0.0 1.8133657656175948 0.0 76-77 1.298445544275911E-4 1.7312607257012147E-4 0.0 2.0138782187924025 0.0 78-79 1.298445544275911E-4 1.7312607257012147E-4 0.0 2.24201510092168 0.0 80-81 1.298445544275911E-4 1.9476683164138664E-4 0.0 2.4948116280126644 0.0 82-83 1.298445544275911E-4 1.9476683164138664E-4 0.0 2.766608741568219 0.0 84-85 1.298445544275911E-4 1.9476683164138664E-4 0.0 3.036663774018537 0.0 86-87 1.298445544275911E-4 1.9476683164138664E-4 0.0 3.3253406796496794 0.0 88-89 1.298445544275911E-4 1.9476683164138664E-4 0.0 3.64786373246828 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4495 0.0 41.210835 1 TACCTGG 4620 0.0 38.63665 2 GTACCTG 4915 0.0 37.979916 1 GTATCAA 7600 0.0 33.208843 1 ATCAACG 7780 0.0 30.69319 3 TCAACGC 7805 0.0 30.594877 4 CAACGCA 8625 0.0 27.903593 5 CCCGAGG 1815 0.0 27.464159 3 ACCTGGG 6590 0.0 26.726503 3 TATCAAC 9130 0.0 26.570744 2 CCGAGGG 1860 0.0 25.77876 4 GTACATG 16245 0.0 25.385733 1 AACGCAG 9775 0.0 25.235449 6 TACATGG 15695 0.0 25.075342 2 ACATGGG 15940 0.0 24.183622 3 TCTGTCG 1025 0.0 22.220541 8 CGCAGAG 10445 0.0 21.987417 8 ACGCAGA 11015 0.0 21.836626 7 CGAGGGC 2240 0.0 21.191576 5 CATGGGG 9530 0.0 20.125286 4 >>END_MODULE