FastQCFastQC Report
Wed 25 May 2016
SRR2049424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620910
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.12752899320696573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.10824707687446845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.047.365451
TACCTGG47400.040.485712
GTACCTG51250.038.025551
GTATCAA101600.034.6197171
CCGAGGG20250.034.0120124
CCCGAGG22050.031.8817733
ATCAACG110050.031.3786133
TCAACGC110550.031.1507574
CGAGGGC23500.029.5113095
CAACGCA117700.029.3804455
ACCTGGG67200.028.2028483
TATCAAC123300.028.1613222
AACGCAG128600.027.2964976
TATAACG4300.024.3026142
GTACATG160900.024.1352331
ATAACGC4800.023.7497673
TACATGG154450.023.6810842
CGCAGAG146300.023.5394948
GCCCCGA31200.023.3089311
ACGCAGA151100.023.0117727
Produced by FastQC (version 0.11.5)