##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4620910 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.938265839412583 31.0 31.0 34.0 30.0 34.0 2 32.13648480494102 33.0 31.0 34.0 30.0 34.0 3 32.25442650906423 33.0 31.0 34.0 30.0 34.0 4 35.9381349128202 37.0 35.0 37.0 35.0 37.0 5 35.79519315459509 37.0 35.0 37.0 35.0 37.0 6 35.861438764226094 37.0 35.0 37.0 35.0 37.0 7 35.830979828648466 37.0 35.0 37.0 35.0 37.0 8 35.8729029563441 37.0 35.0 37.0 35.0 37.0 9 37.610829468654444 39.0 37.0 39.0 35.0 39.0 10-11 37.53300778418104 39.0 37.0 39.0 35.0 39.0 12-13 37.553199478024894 39.0 37.0 39.0 35.0 39.0 14-15 38.91747783445252 40.0 38.0 41.0 35.0 41.0 16-17 38.964478749856625 40.0 38.0 41.0 35.5 41.0 18-19 39.00221612193269 40.0 38.0 41.0 35.5 41.0 20-21 39.02321880322275 40.0 38.5 41.0 35.0 41.0 22-23 38.9851057475692 40.0 38.0 41.0 35.0 41.0 24-25 38.99407941292949 40.0 38.0 41.0 35.0 41.0 26-27 38.938320157717854 40.0 38.0 41.0 35.0 41.0 28-29 38.88735389782532 40.0 38.0 41.0 35.0 41.0 30-31 38.80389847454289 40.0 38.0 41.0 35.0 41.0 32-33 38.76058146555549 40.0 38.0 41.0 35.0 41.0 34-35 38.68990739919193 40.0 38.0 41.0 35.0 41.0 36-37 38.64748534379592 40.0 38.0 41.0 34.5 41.0 38-39 38.60130904951622 40.0 38.0 41.0 34.0 41.0 40-41 38.52238065662391 40.0 38.0 41.0 34.0 41.0 42-43 38.45870596917057 40.0 38.0 41.0 34.0 41.0 44-45 38.40217435526769 40.0 38.0 41.0 34.0 41.0 46-47 38.29421791378755 40.0 38.0 41.0 33.5 41.0 48-49 38.19219287975745 40.0 37.5 41.0 33.0 41.0 50-51 38.088659701227684 40.0 37.0 41.0 33.0 41.0 52-53 37.92719085634648 40.0 37.0 41.0 33.0 41.0 54-55 37.76204329883075 40.0 36.5 41.0 33.0 41.0 56-57 37.57915724391949 39.5 36.0 41.0 33.0 41.0 58-59 37.384513872808604 39.0 36.0 41.0 32.0 41.0 60-61 37.146732245380235 39.0 35.0 41.0 32.0 41.0 62-63 36.88414879320307 39.0 35.0 40.5 31.5 41.0 64-65 36.61434782326425 38.0 35.0 40.0 31.0 41.0 66-67 36.31301767400793 37.5 35.0 40.0 31.0 41.0 68-69 36.00193728075206 37.0 35.0 40.0 31.0 41.0 70-71 35.68735584549364 36.5 34.5 39.0 31.0 41.0 72-73 35.33301492563153 36.0 34.0 39.0 30.0 41.0 74-75 35.01249472506498 36.0 34.0 39.0 30.0 40.0 76-77 34.66481753593989 35.0 34.0 37.5 30.0 40.0 78-79 34.305575741574714 35.0 34.0 37.0 29.5 39.0 80-81 33.982608295768586 35.0 34.0 37.0 29.0 39.0 82-83 33.91840254408764 35.0 34.0 36.0 30.0 39.0 84-85 33.71042392082945 35.0 34.0 36.0 30.0 37.0 86-87 33.480933301016464 35.0 34.0 36.0 29.5 37.0 88-89 33.253369033372216 35.0 34.0 35.0 29.0 37.0 90-91 33.04759030147741 35.0 34.0 35.0 29.0 36.0 92-93 32.88407088647041 35.0 33.0 35.0 29.0 36.0 94-95 32.71245771936697 35.0 33.0 35.0 29.0 36.0 96-97 32.55340560625504 35.0 33.0 35.0 29.0 36.0 98-99 32.36790383279484 35.0 33.0 35.0 29.0 35.0 100-101 31.981280526995768 35.0 32.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08447089849228462 1101 2 0.14262099096469427 1101 3 0.1321122958533536 1101 4 0.09609785260545323 1101 5 0.10588942362658571 1101 6 0.20390295500381939 1101 7 0.17898147354031124 1101 8 0.20929814989969486 1101 9 0.2613372226160138 1101 10-11 0.26272926041544054 1101 12-13 0.19288487888601935 1101 14-15 0.2807080477576349 1101 16-17 0.26160518186451753 1101 18-19 0.2942377772827456 1101 20-21 0.2655014635148589 1101 22-23 0.2863362115519976 1101 24-25 0.25084460742452563 1101 26-27 0.30388572144133263 1101 28-29 0.30530653190824353 1101 30-31 0.33815719926832344 1101 32-33 0.3340496907601178 1101 34-35 0.34578457900530424 1101 36-37 0.3780514313378802 1101 38-39 0.3195283191606251 1101 40-41 0.38722143348859106 1101 42-43 0.36866728277693284 1101 44-45 0.28163489096072425 1101 46-47 0.2866012982536432 1101 48-49 0.34378655100229594 1101 50-51 0.3057950864708445 1101 52-53 0.2925407857578435 1101 54-55 0.22911030945884647 1101 56-57 0.20400024067090783 1101 58-59 0.261429338827611 1101 60-61 0.25900701824203765 1101 62-63 0.15717104801765203 1101 64-65 0.21194103045092305 1101 66-67 0.1904859423974088 1101 68-69 0.17325789898666955 1101 70-71 0.08536730231472234 1101 72-73 0.1462264012310328 1101 74-75 0.09443372775624681 1101 76-77 0.0738348315947377 1101 78-79 0.12118328569648185 1101 80-81 0.11296040414436703 1101 82-83 0.09325174951806758 1101 84-85 0.12141456826578434 1101 86-87 0.038734032407852226 1101 88-89 0.027988289951785816 1101 90-91 0.03319102553391673 1101 92-93 -0.03916373096522108 1101 94-95 -0.04756004670743863 1101 96-97 -0.06854668558025878 1101 98-99 -0.07907327411500376 1101 100-101 -0.18659145692883783 1102 1 0.0325535478729968 1102 2 0.07883828837339735 1102 3 0.06728692018259608 1102 4 0.015419530595060849 1102 5 0.029106493435854475 1102 6 0.1294089345322007 1102 7 0.16749415981882265 1102 8 0.16956033445860896 1102 9 0.14933775749224054 1102 10-11 0.19888761920852716 1102 12-13 0.17445421190495836 1102 14-15 0.14409712584882328 1102 16-17 0.17180375329861164 1102 18-19 0.14404141668079973 1102 20-21 0.12684099563239215 1102 22-23 0.15538847900306507 1102 24-25 0.25961696971272374 1102 26-27 0.3088970742946344 1102 28-29 0.09330773596504116 1102 30-31 0.09228625801922163 1102 32-33 0.0296811785194393 1102 34-35 -0.011240689370247026 1102 36-37 0.15833343261623156 1102 38-39 0.2452063444294268 1102 40-41 0.27957729013941446 1102 42-43 0.12111856145615008 1102 44-45 0.05992435342690072 1102 46-47 0.08562697914097583 1102 48-49 0.18217123844951288 1102 50-51 0.1475624036837715 1102 52-53 0.2038057832553477 1102 54-55 0.18713550919949284 1102 56-57 0.24869602084161357 1102 58-59 0.29727306959476607 1102 60-61 0.23064518477466578 1102 62-63 0.30436977229025075 1102 64-65 0.22895538552531036 1102 66-67 0.2722261934997121 1102 68-69 0.1794709772411167 1102 70-71 0.21221084114606015 1102 72-73 0.07987376234377308 1102 74-75 0.05648661417008327 1102 76-77 0.0439369263094207 1102 78-79 0.019022843039969928 1102 80-81 0.05042876682806963 1102 82-83 -0.025558949598483593 1102 84-85 -0.0663980322210378 1102 86-87 -0.07316884049946992 1102 88-89 -0.08151545353799605 1102 90-91 -0.15928938259521175 1102 92-93 -0.17647746806402864 1102 94-95 -0.1432975459363206 1102 96-97 -0.19001226595550236 1102 98-99 -0.18336030630461408 1102 100-101 -0.17222246240991268 1103 1 0.050691669929349814 1103 2 0.13262317198657314 1103 3 0.03746808243910493 1103 4 0.05052224467797117 1103 5 0.08243499339835836 1103 6 0.08005650899307426 1103 7 0.1598084824062056 1103 8 0.15910214200844308 1103 9 0.1974845122064366 1103 10-11 0.2027559320365242 1103 12-13 0.09279594707153649 1103 14-15 0.19985647872245949 1103 16-17 0.19938647928691466 1103 18-19 0.21262080213784884 1103 20-21 0.2674274337652278 1103 22-23 0.2903218908610796 1103 24-25 0.1495635440474672 1103 26-27 0.15194379645356548 1103 28-29 0.279338013063267 1103 30-31 0.29011089705893056 1103 32-33 0.29361644195313374 1103 34-35 0.3189501252909892 1103 36-37 0.22601418568365972 1103 38-39 0.17818696055829975 1103 40-41 0.20340657731679812 1103 42-43 0.2255236920341872 1103 44-45 0.18989772114175452 1103 46-47 0.19636722857387667 1103 48-49 0.20900696465592006 1103 50-51 0.10692361359155456 1103 52-53 0.046273384590932665 1103 54-55 0.13969338863272185 1103 56-57 0.26970894542953516 1103 58-59 0.18963735899736633 1103 60-61 0.15220011968877145 1103 62-63 0.19335235737518275 1103 64-65 0.10942345560037836 1103 66-67 0.1309213382142289 1103 68-69 0.05471644181127999 1103 70-71 -0.03506921473982061 1103 72-73 -0.012530202563482362 1103 74-75 -0.0026151076928613293 1103 76-77 0.09332520721245885 1103 78-79 0.10627700799044248 1103 80-81 0.07919159176778834 1103 82-83 0.034673291497831826 1103 84-85 -0.015670512419845295 1103 86-87 -0.038044894723405775 1103 88-89 -0.13278737271961205 1103 90-91 -0.12063993958893349 1103 92-93 -0.0921725729740146 1103 94-95 -0.11237129748734986 1103 96-97 -0.0363142993417398 1103 98-99 -0.0075884365072056426 1103 100-101 -0.04599901053074262 1104 1 -0.051254662260344475 1104 2 0.03910886746039921 1104 3 0.008995244609842246 1104 4 0.020296366543981037 1104 5 0.05543401048638685 1104 6 0.14405961981993443 1104 7 0.11530752776591413 1104 8 0.1601628796227743 1104 9 0.22100097166265442 1104 10-11 0.008234130961071173 1104 12-13 0.024760215284302944 1104 14-15 0.06860374579368766 1104 16-17 0.16081317255618188 1104 18-19 0.15412817492001807 1104 20-21 0.17290761956041223 1104 22-23 0.18309075765059646 1104 24-25 0.17661072366496455 1104 26-27 0.20554507015544488 1104 28-29 0.24482021949803112 1104 30-31 0.20227531225300766 1104 32-33 0.14893375932909692 1104 34-35 0.16033443381917323 1104 36-37 0.24726249095369468 1104 38-39 0.2602193713658707 1104 40-41 0.19873411333334445 1104 42-43 0.1801357527944205 1104 44-45 0.2158078296170416 1104 46-47 0.1873660763459739 1104 48-49 0.12666888597395598 1104 50-51 0.05727913778300575 1104 52-53 0.0753468188583426 1104 54-55 0.03344487289576392 1104 56-57 0.13296610805890197 1104 58-59 0.17993122109064075 1104 60-61 0.20166950743105616 1104 62-63 0.12299473558236684 1104 64-65 0.1310614584514198 1104 66-67 0.08598038596760205 1104 68-69 0.0630077393493238 1104 70-71 0.1383383359340229 1104 72-73 0.10884660533172763 1104 74-75 -0.0063554112929793405 1104 76-77 0.03500898347920156 1104 78-79 0.08634820471167615 1104 80-81 0.06401492362889627 1104 82-83 0.05458299176151371 1104 84-85 -0.03639255220249282 1104 86-87 -0.05134658131741787 1104 88-89 -0.033893125126319035 1104 90-91 -0.061184563603909226 1104 92-93 -0.02311438813504907 1104 94-95 -0.039578502649881386 1104 96-97 -0.001722214091948615 1104 98-99 -0.08047494017404233 1104 100-101 -0.04820641987760865 1105 1 -0.36597153126533755 1105 2 -0.17199561552554954 1105 3 -0.3126901294683684 1105 4 -0.2949634862834998 1105 5 -0.36209009420627325 1105 6 -0.2506773734033416 1105 7 -0.20237609529621636 1105 8 -0.1620680059527686 1105 9 -0.13519293113406405 1105 10-11 -0.25141843350493787 1105 12-13 -0.2797088586384575 1105 14-15 -0.3988952760206885 1105 16-17 -0.3441050569170372 1105 18-19 -0.3628086085700559 1105 20-21 -0.31623202912518167 1105 22-23 -0.2738729276620333 1105 24-25 -0.1573818379033014 1105 26-27 -0.12934307360652753 1105 28-29 -0.21290991572619333 1105 30-31 -0.036732075232578154 1105 32-33 -0.04696647722199998 1105 34-35 -0.12949369705214764 1105 36-37 -0.0402713823433416 1105 38-39 -0.21302125159848373 1105 40-41 -0.0629257685304978 1105 42-43 -0.19918257109113569 1105 44-45 -0.17309039421774486 1105 46-47 -0.24499584782024186 1105 48-49 -0.18980663121286057 1105 50-51 -0.15236304849181437 1105 52-53 -0.15977898032864601 1105 54-55 -0.0915262060866624 1105 56-57 -0.1256413610190492 1105 58-59 -0.1561121068882727 1105 60-61 -0.12264045205940022 1105 62-63 -0.14543941303981 1105 64-65 -0.1016817127036802 1105 66-67 -0.3291739253916788 1105 68-69 -0.23931935100342372 1105 70-71 -0.2588872390702903 1105 72-73 -0.2387893634519287 1105 74-75 -0.15081514557986964 1105 76-77 -0.24232470336868062 1105 78-79 -0.20932259749280746 1105 80-81 -0.2537204063352476 1105 82-83 -0.18134497608214417 1105 84-85 -0.09280357981472065 1105 86-87 -0.2542195684538626 1105 88-89 -0.3155014782524077 1105 90-91 -0.20900026479366574 1105 92-93 -0.33102404471679137 1105 94-95 -0.24465817447031668 1105 96-97 -0.3293046290531123 1105 98-99 -0.33066552194958376 1105 100-101 -0.39419616065891105 1106 1 0.015718800332475524 1106 2 0.05288326073122107 1106 3 0.005465809283151657 1106 4 0.036485099609791405 1106 5 0.007530561211034126 1106 6 0.16318611954931583 1106 7 0.11930825739468531 1106 8 0.13675058696804143 1106 9 0.05336804156860353 1106 10-11 0.05916936156647523 1106 12-13 0.02198943752244986 1106 14-15 0.09260788694429323 1106 16-17 0.07925450763145392 1106 18-19 0.03110789682817483 1106 20-21 0.10712062716957149 1106 22-23 0.10177021967582789 1106 24-25 0.11867449069077907 1106 26-27 0.1871888887247195 1106 28-29 0.10490994581606117 1106 30-31 0.04738572804448893 1106 32-33 0.18058073538279729 1106 34-35 0.03011089830890512 1106 36-37 0.07565857935295384 1106 38-39 0.10616554564568759 1106 40-41 0.10490716384794041 1106 42-43 0.0470761602355978 1106 44-45 0.05365306873628839 1106 46-47 0.05412981231182812 1106 48-49 0.08912917890370409 1106 50-51 0.054650418425367775 1106 52-53 0.15309126910623405 1106 54-55 0.14462619344509875 1106 56-57 0.12164813544557518 1106 58-59 0.1659204708026749 1106 60-61 0.1476346133665487 1106 62-63 0.19817717495639187 1106 64-65 0.1299892956215416 1106 66-67 0.16298358456746342 1106 68-69 0.13150205693743544 1106 70-71 0.12401944696874523 1106 72-73 0.048542145464487874 1106 74-75 0.10281978032922012 1106 76-77 0.16376214967131375 1106 78-79 0.1713318694622501 1106 80-81 0.12114947168763024 1106 82-83 0.11218005071243198 1106 84-85 0.13980567906105534 1106 86-87 0.1321220133733405 1106 88-89 0.12325324646220537 1106 90-91 0.012865317532323672 1106 92-93 0.02680726725604643 1106 94-95 0.11008456461074445 1106 96-97 0.10653068163171042 1106 98-99 0.07014278341446811 1106 100-101 0.10816300799529444 1107 1 0.05924451515098994 1107 2 0.08767783099867898 1107 3 0.109971886917684 1107 4 0.040134909605541225 1107 5 0.06587654405948484 1107 6 0.13894600693399184 1107 7 0.07124107025357063 1107 8 0.1396057766652845 1107 9 0.13746043525832619 1107 10-11 0.09166928993111867 1107 12-13 0.0052066609360679195 1107 14-15 -0.002113274139745158 1107 16-17 0.03239863569272927 1107 18-19 0.12193881692177655 1107 20-21 0.10043783534646167 1107 22-23 0.133517681168982 1107 24-25 0.14523733536486105 1107 26-27 0.08086391891390576 1107 28-29 0.04442157007384395 1107 30-31 0.16907576754626774 1107 32-33 0.22282509154014463 1107 34-35 0.2199209395390298 1107 36-37 0.18559853755429856 1107 38-39 0.1632132593407789 1107 40-41 0.12514214600733453 1107 42-43 0.12961727324435657 1107 44-45 0.18682679698131466 1107 46-47 0.2655837371333618 1107 48-49 0.1766682929449246 1107 50-51 0.1918898237836899 1107 52-53 0.21036580535258054 1107 54-55 0.26254487480876065 1107 56-57 0.2863026233437438 1107 58-59 0.2456472558306615 1107 60-61 0.17386352357674895 1107 62-63 0.07842025304803002 1107 64-65 0.24390292839824212 1107 66-67 0.1661961050631362 1107 68-69 0.1582926611077511 1107 70-71 0.1950078465894194 1107 72-73 0.21670361837836793 1107 74-75 0.21502464380133546 1107 76-77 0.19535741673474405 1107 78-79 0.21487162935873272 1107 80-81 0.1880095098476673 1107 82-83 0.17689373897810157 1107 84-85 0.16142586613167254 1107 86-87 0.17621296071111914 1107 88-89 0.2387236633344898 1107 90-91 0.20493069445937095 1107 92-93 0.22491624851032554 1107 94-95 0.301756606582245 1107 96-97 0.2585564521666157 1107 98-99 0.18464137003752512 1107 100-101 0.1797341146137228 1108 1 0.12078016217816412 1108 2 0.1515594559650495 1108 3 0.1581494494681266 1108 4 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-0.1948889424246758 2213 88-89 -0.07112683431261502 2213 90-91 -0.09138205280252265 2213 92-93 -0.2026049691634384 2213 94-95 -0.10485410428270825 2213 96-97 -0.061892221468497155 2213 98-99 -0.11848318749380837 2213 100-101 -0.025413597042465597 2214 1 0.06014406041762754 2214 2 0.006185892180987196 2214 3 0.05783753387923696 2214 4 0.030624781453411742 2214 5 -0.002549665117861366 2214 6 -0.02783200050710377 2214 7 -0.0670970860093476 2214 8 -0.13216685665427264 2214 9 -0.20708772513853546 2214 10-11 -0.16383277703870647 2214 12-13 -0.18692955237521858 2214 14-15 -0.22140678385449775 2214 16-17 -0.25030950816836395 2214 18-19 -0.2543875576131569 2214 20-21 -0.16999188767957918 2214 22-23 -0.18424875124110685 2214 24-25 -0.17025770390756634 2214 26-27 -0.2085873005982819 2214 28-29 -0.19822869863223502 2214 30-31 -0.16990811008911777 2214 32-33 -0.18288305232096036 2214 34-35 -0.2036011770380597 2214 36-37 -0.32330948228861445 2214 38-39 -0.23028612056827313 2214 40-41 -0.14658212021838324 2214 42-43 -0.20594886978995675 2214 44-45 -0.13786853166688928 2214 46-47 -0.23739786683521658 2214 48-49 -0.2821965272287059 2214 50-51 -0.33695846063135804 2214 52-53 -0.20096842366767476 2214 54-55 -0.20328852742356673 2214 56-57 -0.08393528488279145 2214 58-59 -0.2330278165132853 2214 60-61 -0.21905917175190126 2214 62-63 -0.23499066055263285 2214 64-65 -0.15449310301970343 2214 66-67 -0.1609066233370271 2214 68-69 -0.19701387051689068 2214 70-71 -0.16491030140562657 2214 72-73 -0.23674408728513185 2214 74-75 -0.05239929170920732 2214 76-77 -0.011708106208615732 2214 78-79 -0.11078504695380076 2214 80-81 -0.16210851412918004 2214 82-83 -0.05390390467629658 2214 84-85 -0.12337570476540094 2214 86-87 -0.08617542291847968 2214 88-89 -0.08164115683913309 2214 90-91 -0.11641965692549405 2214 92-93 -0.13766684367653426 2214 94-95 -0.10901602673730793 2214 96-97 -0.15071990169862204 2214 98-99 -0.13975094083473039 2214 100-101 -0.26868920080587344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 23.0 12 163.0 13 586.0 14 1341.0 15 2299.0 16 3365.0 17 4530.0 18 6803.0 19 10023.0 20 10544.0 21 10446.0 22 10952.0 23 11783.0 24 13905.0 25 17170.0 26 22370.0 27 29607.0 28 38428.0 29 50937.0 30 67742.0 31 89330.0 32 119154.0 33 162939.0 34 231631.0 35 348258.0 36 577248.0 37 1022886.0 38 1301261.0 39 454379.0 40 805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.330260103234025 20.36618911311287 11.820939179753537 25.482611603899567 2 15.577772651273591 21.290316577022576 38.06135040488082 25.070560366823013 3 17.834279395184065 26.01011489078991 29.683179287196676 26.472426426829347 4 12.80312752250098 17.986825105877415 37.66509194076492 31.544955430856692 5 12.466808026062761 38.06367924834543 34.538091076828785 14.931421648763019 6 29.548984940195762 38.55218993661422 18.330458719170036 13.568366404019988 7 26.86364806931968 33.10640112012569 22.32423916501295 17.70571164554168 8 25.64570614878888 35.68584975686607 20.40057045040912 18.267873643935932 9 25.155499674306576 17.395491364255093 22.01596655204278 35.433042409395554 10-11 23.617689984444425 27.097427054577818 28.831046742029393 20.453836218948368 12-13 24.24510540131706 24.45471779368133 29.084347022556162 22.215829782445446 14-15 21.654187162268904 26.83963764713011 26.45460526173416 25.051569928866822 16-17 21.05703205645641 30.080568546022317 26.92950089917354 21.93289849834773 18-19 21.065125700349068 28.72939529226927 29.228918113531748 20.976560893849914 20-21 22.220201215777845 28.25324449080376 28.571266265735535 20.955288027682858 22-23 21.923733636881046 28.189555736857024 28.67053242759543 21.216178198666498 24-25 21.395287941119822 27.927713372474255 28.826259329872254 21.85073935653367 26-27 21.229119372591114 28.198385166558104 28.760157631289076 21.81233782956171 28-29 21.36736044881271 27.96670910425949 29.104592760332775 21.56133768659502 30-31 21.79224438476404 28.50820509380187 28.46365759125367 21.23589293018042 32-33 21.131281500829925 28.323035938808587 29.022908907552843 21.522773652808645 34-35 21.41848683484422 27.91590833840088 28.88744857614626 21.778156250608646 36-37 22.03107180187452 28.077553988283693 28.435189172695424 21.456185037146362 38-39 21.580835809396852 28.447697531438614 28.761391154556136 21.2100755046084 40-41 21.228513431337117 27.828663618205074 29.236286791995514 21.706536158462296 42-43 21.09375304323438 28.19126811928224 28.878265431064815 21.836713406418568 44-45 21.376081767444074 28.291829964227826 28.852866643150378 21.479221625177725 46-47 21.57850942779669 28.62653676440355 28.44676697879855 21.348186829001214 48-49 21.365585999294513 28.22356418973752 29.009026360608626 21.401823450359345 50-51 21.437671367760895 28.040515829133223 28.780618968990957 21.741193834114924 52-53 21.434652481870454 28.043632098439485 28.72456940299638 21.79714601669368 54-55 21.618847802705528 28.625508828347662 28.513485439015255 21.24215792993155 56-57 21.4408309185853 28.266856528259588 28.49709256401878 21.79521998913634 58-59 21.21520436450829 27.92425085102285 28.91895752135402 21.941587263114844 60-61 21.58717655180473 28.168163846515082 28.45587773836755 21.788781863312636 62-63 21.508782902069072 28.127652345533672 28.638060468608995 21.725504283788258 64-65 21.48807269563787 28.19457639296156 28.6158029479042 21.701547963496367 66-67 21.38283368427431 28.053435362298767 28.603132283467975 21.960598669958948 68-69 21.703463170674176 28.477691623511387 28.348214961988006 21.470630243826434 70-71 21.76855857396054 27.833879041141245 28.489886191248043 21.90767619365017 72-73 21.13926694092722 28.04867439530309 28.96031301193921 21.851745651830484 74-75 21.223059960051156 28.00174640925705 28.88409425849021 21.89109937220158 76-77 21.41072862271717 28.341657811989414 28.510877727547175 21.736735837746245 78-79 21.350242701112983 28.085409583826564 28.93686524948549 21.627482465574964 80-81 21.37524859821983 27.98468267072936 28.754855645316614 21.885213085734197 82-83 21.18886756071856 27.948369477007773 29.111084180388712 21.751678781884955 84-85 21.11384986939802 27.899764332133714 28.843853266997193 22.142532531471073 86-87 21.307848454092376 27.980386980053712 28.848625054372405 21.863139511481506 88-89 21.093897089534313 27.970324027085574 28.927981717886738 22.007797165493376 90-91 21.02753570184228 27.782482238346994 28.923545362277125 22.266436697533603 92-93 21.28947544964087 27.955597490537578 28.572077794200712 22.182849265620842 94-95 21.18679004784772 27.69035752698062 28.93818533578884 22.184667089382827 96-97 21.088908894568387 27.930277802424197 28.73163511083315 22.24917819217427 98-99 21.018197714303028 27.80924103693861 28.806155064695048 22.36640618406331 100-101 21.05485716016975 27.93262582478343 28.419077627566864 22.593439387479954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 313.0 1 184.5 2 180.5 3 655.5 4 654.5 5 348.0 6 793.5 7 2066.5 8 3473.0 9 3812.5 10 2610.5 11 1373.5 12 1192.5 13 1272.5 14 1349.0 15 1423.5 16 1517.5 17 1721.0 18 2150.5 19 2719.5 20 3761.5 21 5474.5 22 8721.5 23 14928.5 24 21169.5 25 26642.5 26 35269.5 27 47494.0 28 64226.0 29 78994.5 30 85903.0 31 95804.5 32 110141.5 33 121878.5 34 132052.5 35 140377.0 36 145197.0 37 149887.0 38 154743.0 39 157953.5 40 162178.0 41 161761.0 42 156855.0 43 154646.0 44 153192.0 45 151144.5 46 149072.0 47 147704.0 48 145518.5 49 144505.0 50 145820.5 51 139952.0 52 127049.0 53 118813.0 54 116099.0 55 114954.0 56 110207.5 57 100226.0 58 90636.0 59 82207.5 60 70875.0 61 57151.0 62 44674.0 63 34452.5 64 26846.5 65 21409.5 66 17019.0 67 13094.5 68 9650.5 69 6764.0 70 4824.0 71 3413.0 72 2459.5 73 1782.0 74 1319.0 75 907.0 76 544.5 77 373.0 78 252.0 79 135.0 80 71.5 81 32.5 82 20.5 83 12.5 84 6.5 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06187093018474715 2 9.52193399135668E-4 3 0.0 4 0.0 5 0.0013850085805609718 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0012551640261333806 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.5148531349885627E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.656303628506074E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4620910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.39554536724122 #Duplication Level Percentage of deduplicated Percentage of total 1 74.33439438490905 40.434599221107206 2 15.330804065460581 16.678548961180944 3 4.7138885401118396 7.6924361381931625 4 1.9313632849046378 4.20230236738617 5 0.9620184903871725 2.6164760218990177 6 0.5868249307029914 1.915239728440965 7 0.39196955102158193 1.4924978246620142 8 0.26969878584667895 1.173633003281032 9 0.2152046191555574 1.0535555362064393 >10 1.1227264871446796 11.227027979865516 >50 0.08604490988927 3.2534974281731697 >100 0.0500771521781143 5.271149858114155 >500 0.0033099066808453424 1.2571854277675085 >1k 0.0015951348638543602 1.495515534935171 >5k 7.975674319261564E-5 0.23633496878734764 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.12752899320696573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.10824707687446845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.1640759071265184E-5 0.0 0.0 0.0 6 0.0 2.1640759071265184E-5 0.0 0.0 0.0 7 0.0 2.1640759071265184E-5 0.0 0.0 0.0 8 0.0 2.1640759071265184E-5 0.0 0.0 0.0 9 2.1640759071265184E-5 2.1640759071265184E-5 0.0 0.0 0.0 10-11 2.1640759071265184E-5 2.1640759071265184E-5 0.0 2.1640759071265184E-5 0.0 12-13 2.1640759071265184E-5 2.1640759071265184E-5 0.0 3.2461138606897776E-5 0.0 14-15 2.1640759071265184E-5 2.1640759071265184E-5 0.0 4.328151814253037E-5 0.0 16-17 2.1640759071265184E-5 2.1640759071265184E-5 0.0 4.328151814253037E-5 0.0 18-19 3.2461138606897776E-5 2.1640759071265184E-5 0.0 4.328151814253037E-5 0.0 20-21 4.328151814253037E-5 2.1640759071265184E-5 0.0 4.328151814253037E-5 0.0 22-23 4.328151814253037E-5 3.2461138606897776E-5 0.0 1.298445544275911E-4 0.0 24-25 4.328151814253037E-5 4.328151814253037E-5 0.0 3.6789290421150817E-4 0.0 26-27 4.328151814253037E-5 4.328151814253037E-5 0.0 7.466061879586488E-4 0.0 28-29 4.328151814253037E-5 4.328151814253037E-5 0.0 0.0019043867982713363 0.0 30-31 4.328151814253037E-5 4.328151814253037E-5 0.0 0.005431830526887562 0.0 32-33 4.328151814253037E-5 4.328151814253037E-5 0.0 0.012410975327370583 0.0 34-35 6.492227721379555E-5 4.328151814253037E-5 0.0 0.023436942074180192 0.0 36-37 6.492227721379555E-5 4.328151814253037E-5 0.0 0.04047903984280153 0.0 38-39 6.492227721379555E-5 4.328151814253037E-5 0.0 0.06675092135531746 0.0 40-41 6.492227721379555E-5 4.328151814253037E-5 0.0 0.10034819981345666 0.0 42-43 6.492227721379555E-5 4.328151814253037E-5 0.0 0.13645580632386262 0.0 44-45 7.574265674942815E-5 4.328151814253037E-5 0.0 0.17659941440105953 0.0 46-47 8.656303628506074E-5 4.328151814253037E-5 0.0 0.22783391150227986 0.0 48-49 8.656303628506074E-5 4.328151814253037E-5 0.0 0.2847923893778498 0.0 50-51 8.656303628506074E-5 4.328151814253037E-5 0.0 0.3461439413448866 0.0 52-53 8.656303628506074E-5 6.492227721379555E-5 0.0 0.4081771772226683 0.0 54-55 8.656303628506074E-5 7.574265674942815E-5 0.0 0.47949429874202265 0.0 56-57 8.656303628506074E-5 1.298445544275911E-4 0.0 0.5624433282621821 0.0 58-59 8.656303628506074E-5 1.298445544275911E-4 0.0 0.6564075041496156 0.0 60-61 8.656303628506074E-5 1.298445544275911E-4 0.0 0.7583246589957389 0.0 62-63 9.738341582069332E-5 1.5148531349885627E-4 0.0 0.8630875736597337 0.0 64-65 1.0820379535632592E-4 1.5148531349885627E-4 0.0 0.9775130872490483 0.0 66-67 1.0820379535632592E-4 1.6230569303448886E-4 0.0 1.1158083580939686 0.0 68-69 1.298445544275911E-4 1.9476683164138664E-4 0.0 1.267996996262641 0.0 70-71 1.298445544275911E-4 1.9476683164138664E-4 0.0 1.4343495112434563 0.0 72-73 1.406649339632237E-4 1.9476683164138664E-4 0.0 1.6275041063340336 0.0 74-75 1.5148531349885627E-4 1.9476683164138664E-4 0.0 1.8253222850044688 0.0 76-77 1.5148531349885627E-4 2.0558721117701925E-4 0.0 2.0279122510501177 0.0 78-79 1.5148531349885627E-4 2.1640759071265184E-4 0.0 2.2586460242679474 0.0 80-81 1.5148531349885627E-4 2.1640759071265184E-4 0.0 2.514169287001911 0.0 82-83 1.5148531349885627E-4 2.1640759071265184E-4 0.0 2.7891367717614064 0.0 84-85 1.5148531349885627E-4 2.1640759071265184E-4 0.0 3.0617886953002764 0.0 86-87 1.5148531349885627E-4 2.1640759071265184E-4 0.0 3.3540579669372486 0.0 88-89 1.5148531349885627E-4 2.1640759071265184E-4 0.0 3.68044389525007 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5710 0.0 47.36545 1 TACCTGG 4740 0.0 40.48571 2 GTACCTG 5125 0.0 38.02555 1 GTATCAA 10160 0.0 34.619717 1 CCGAGGG 2025 0.0 34.012012 4 CCCGAGG 2205 0.0 31.881773 3 ATCAACG 11005 0.0 31.378613 3 TCAACGC 11055 0.0 31.150757 4 CGAGGGC 2350 0.0 29.511309 5 CAACGCA 11770 0.0 29.380445 5 ACCTGGG 6720 0.0 28.202848 3 TATCAAC 12330 0.0 28.161322 2 AACGCAG 12860 0.0 27.296497 6 TATAACG 430 0.0 24.302614 2 GTACATG 16090 0.0 24.135233 1 ATAACGC 480 0.0 23.749767 3 TACATGG 15445 0.0 23.681084 2 CGCAGAG 14630 0.0 23.539494 8 GCCCCGA 3120 0.0 23.308931 1 ACGCAGA 15110 0.0 23.011772 7 >>END_MODULE