FastQCFastQC Report
Wed 25 May 2016
SRR2049423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11379479
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429040.3770295634800152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363910.3197949572208007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205790.18084307726214882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228650.059.3115431
GTATCAA438600.043.179931
ATCAACG542650.034.277873
TCAACGC549850.033.79584
TATCAAC568950.032.8750082
CAACGCA578650.032.137675
TGGTATC91150.031.4734612
AACGCAG606300.030.6959576
GTGGTAT96500.030.3219911
CGCAGAG666450.027.3352538
ACGCAGA673900.027.1810917
GTACATG471400.026.1689741
TACATGG461100.025.5561772
GCAGAGT721000.024.5094179
ACATGGG475250.024.0671843
GAGTACT453050.021.83507312-13
CATGGGT113550.021.2094294
CAGAGTA710400.020.99948910-11
AGAGTAC669650.020.6600310-11
GTACTTT489050.019.98320414-15
Produced by FastQC (version 0.11.5)