##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049423_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11379479 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57856040685167 31.0 31.0 34.0 30.0 34.0 2 31.62359173034196 33.0 31.0 34.0 30.0 34.0 3 31.646946841766656 33.0 31.0 34.0 30.0 34.0 4 35.28259738429149 37.0 35.0 37.0 33.0 37.0 5 35.30649610584105 37.0 35.0 37.0 33.0 37.0 6 35.41194777019229 37.0 35.0 37.0 33.0 37.0 7 35.35906679031615 37.0 35.0 37.0 33.0 37.0 8 35.33468526986165 37.0 35.0 37.0 33.0 37.0 9 36.93413274895977 39.0 37.0 39.0 33.0 39.0 10-11 36.86659477116659 39.0 37.0 39.0 33.0 39.0 12-13 36.8348713064983 39.0 37.0 39.0 33.0 39.0 14-15 38.01625456666338 40.0 38.0 41.0 33.0 41.0 16-17 38.00543087253819 40.0 38.0 41.0 33.0 41.0 18-19 38.039377330016606 40.0 38.0 41.0 33.0 41.0 20-21 38.0133314099881 40.0 38.0 41.0 33.0 41.0 22-23 37.907861686813604 40.0 38.0 41.0 33.0 41.0 24-25 37.8041493375927 40.0 38.0 41.0 33.0 41.0 26-27 37.66999991827394 40.0 38.0 41.0 32.5 41.0 28-29 37.52134504576176 40.0 37.0 41.0 32.0 41.0 30-31 37.33624694065519 40.0 37.0 41.0 32.0 41.0 32-33 37.08964997430902 40.0 36.5 41.0 31.5 41.0 34-35 36.89716805136685 40.0 36.0 41.0 31.0 41.0 36-37 36.71332984576887 39.0 36.0 41.0 30.5 41.0 38-39 36.50985510848081 39.0 36.0 41.0 30.0 41.0 40-41 36.296228061056226 39.0 35.0 40.0 30.0 41.0 42-43 36.063222797809985 39.0 35.0 40.0 30.0 41.0 44-45 35.77479487417658 39.0 35.0 40.0 28.5 41.0 46-47 35.40510637613549 39.0 35.0 40.0 27.5 41.0 48-49 35.09282182426806 38.5 35.0 40.0 26.0 41.0 50-51 33.750532867102265 37.0 33.0 39.0 24.0 40.0 52-53 34.06432741780182 37.5 33.5 39.5 25.0 40.0 54-55 35.120142890548856 39.0 35.0 40.0 27.0 41.0 56-57 35.24843703301355 39.0 35.0 40.5 27.0 41.0 58-59 35.095417593371366 39.0 35.0 40.0 26.0 41.0 60-61 34.8435619504197 38.5 35.0 40.0 26.0 41.0 62-63 34.57884701927039 38.0 34.0 40.0 26.0 41.0 64-65 34.30093517462443 38.0 34.0 40.0 25.0 41.0 66-67 33.9799828269818 37.0 34.0 40.0 24.5 41.0 68-69 33.63388798379961 37.0 34.0 39.5 23.0 41.0 70-71 33.28887245189345 36.0 33.0 39.0 22.5 41.0 72-73 33.01853318592178 36.0 33.0 39.0 22.0 41.0 74-75 32.81145375812021 35.5 33.0 39.0 22.0 40.0 76-77 32.468653485805454 35.0 33.0 37.5 20.5 40.0 78-79 32.08619296190977 35.0 33.0 37.0 20.0 39.0 80-81 31.73203087768781 35.0 32.5 37.0 18.5 39.0 82-83 31.38177828703757 35.0 32.0 36.0 17.5 38.5 84-85 31.055328763293996 35.0 32.0 36.0 13.5 37.0 86-87 30.75453186389289 35.0 32.0 35.5 8.5 37.0 88-89 30.458990477507804 35.0 31.5 35.0 6.5 36.5 90-91 30.18442834685138 35.0 31.0 35.0 2.0 36.0 92-93 29.931916346961053 34.0 31.0 35.0 2.0 36.0 94-95 29.667069643522343 34.0 31.0 35.0 2.0 36.0 96-97 29.409638086242786 34.0 31.0 35.0 2.0 35.0 98-99 29.083719562204912 34.0 31.0 35.0 2.0 35.0 100-101 28.53629432419533 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38805850554744126 1101 2 0.4460707705887579 1101 3 0.44827794976844615 1101 4 0.4174245182925347 1101 5 0.4355056614564816 1101 6 0.42183438311798227 1101 7 0.4178244479724569 1101 8 0.4350872977506839 1101 9 0.4706385778870512 1101 10-11 0.44908096379077733 1101 12-13 0.46184335597138215 1101 14-15 0.5718101133389908 1101 16-17 0.5464127332397197 1101 18-19 0.5423740038164269 1101 20-21 0.44843054153533046 1101 22-23 0.48093004587494903 1101 24-25 0.47886253957587854 1101 26-27 0.45132392448584824 1101 28-29 0.4276437591230149 1101 30-31 0.37456942294733153 1101 32-33 0.4136174284388048 1101 34-35 0.3674349276884783 1101 36-37 0.31256329612686784 1101 38-39 0.2637655745424681 1101 40-41 0.27989677668832513 1101 42-43 0.19403734864502553 1101 44-45 0.1294668801023775 1101 46-47 -0.022801852107541265 1101 48-49 -0.07125546656120463 1101 50-51 -0.13916013889075884 1101 52-53 -0.008299518233698677 1101 54-55 0.18051202269202804 1101 56-57 0.21582747043390782 1101 58-59 0.21593275946506907 1101 60-61 0.2100224889469331 1101 62-63 0.11434415929441144 1101 64-65 0.13324048559109514 1101 66-67 0.10770879052484617 1101 68-69 0.09267537478309151 1101 70-71 0.017660027740774353 1101 72-73 0.01990069720784504 1101 74-75 0.054796902419617766 1101 76-77 0.08717452892333455 1101 78-79 0.05001922105968504 1101 80-81 0.009359416183947644 1101 82-83 -0.039712127090453464 1101 84-85 -0.17036234061664857 1101 86-87 -0.18788234405285564 1101 88-89 -0.2302449271632554 1101 90-91 -0.239459505983163 1101 92-93 -0.2182802469975762 1101 94-95 -0.4410192242828934 1101 96-97 -0.4685918268819549 1101 98-99 -0.561976156417419 1101 100-101 -0.6883702117961406 1102 1 0.3761941619300657 1102 2 0.3816227074227889 1102 3 0.35931479691807056 1102 4 0.33248577936321055 1102 5 0.37185769655924616 1102 6 0.38727267204534144 1102 7 0.34801374174237765 1102 8 0.3860313751626663 1102 9 0.46359989461195994 1102 10-11 0.47271302579180485 1102 12-13 0.45468358361328853 1102 14-15 0.5516871375133903 1102 16-17 0.4806980815459667 1102 18-19 0.4653823951217575 1102 20-21 0.4403346066364904 1102 22-23 0.43928038348344955 1102 24-25 0.4355544037065897 1102 26-27 0.47588674040922996 1102 28-29 0.4821167718067727 1102 30-31 0.40743908118219707 1102 32-33 0.4049774578813725 1102 34-35 0.3710050053647649 1102 36-37 0.3056819835536828 1102 38-39 0.3145871711466057 1102 40-41 0.3120043856081054 1102 42-43 0.2562691412468183 1102 44-45 0.2560295136007298 1102 46-47 0.2747470137137995 1102 48-49 0.173969715749422 1102 50-51 0.15232503835389366 1102 52-53 0.29266762310162875 1102 54-55 0.3885466962741617 1102 56-57 0.3814662932160928 1102 58-59 0.39863581421171546 1102 60-61 0.4554342173345276 1102 62-63 0.4272037739832868 1102 64-65 0.34759935945098164 1102 66-67 0.32409184083503106 1102 68-69 0.3281105395875059 1102 70-71 0.296689575133648 1102 72-73 0.2789770067196429 1102 74-75 0.21800460660160326 1102 76-77 0.2490633093263881 1102 78-79 0.20980684548804618 1102 80-81 0.17049174451534554 1102 82-83 0.08773209890066269 1102 84-85 0.057561085681662405 1102 86-87 0.060281924125977326 1102 88-89 0.07552457521974532 1102 90-91 -0.036240835960700934 1102 92-93 -0.15129785452300482 1102 94-95 -0.23755014630134497 1102 96-97 -0.3113235196682993 1102 98-99 -0.4120084178623742 1102 100-101 -0.4211805933778976 1103 1 0.2523730625365488 1103 2 0.255354685927891 1103 3 0.31270082320919457 1103 4 0.3005863803487685 1103 5 0.28545793868762814 1103 6 0.3154138371432538 1103 7 0.31117981683204476 1103 8 0.31239727479916723 1103 9 0.37538679899888194 1103 10-11 0.411541238665464 1103 12-13 0.3882139648369005 1103 14-15 0.4032110300383991 1103 16-17 0.44536769731052317 1103 18-19 0.4154792640807088 1103 20-21 0.44839939075518487 1103 22-23 0.38622517575357307 1103 24-25 0.38408465021533544 1103 26-27 0.46901411224389733 1103 28-29 0.4547772121203906 1103 30-31 0.3975993224101657 1103 32-33 0.2976960683003682 1103 34-35 0.30979160124360305 1103 36-37 0.2899245474887877 1103 38-39 0.3252710278930877 1103 40-41 0.28052793158418154 1103 42-43 0.27421027879813664 1103 44-45 0.18259291908476172 1103 46-47 0.17957001549520868 1103 48-49 0.10447492264459157 1103 50-51 -0.012707394292831964 1103 52-53 0.15433189922104162 1103 54-55 0.269159209780625 1103 56-57 0.3218670861758568 1103 58-59 0.3666481588734314 1103 60-61 0.3185374197163071 1103 62-63 0.22522165122951776 1103 64-65 0.2981113765999126 1103 66-67 0.2706033447784364 1103 68-69 0.18066871071464163 1103 70-71 0.1536195192145513 1103 72-73 0.1749047079314039 1103 74-75 0.15095565882227646 1103 76-77 0.09880056343597232 1103 78-79 0.1230065820064894 1103 80-81 0.06270853807546217 1103 82-83 0.09570675609216295 1103 84-85 0.0807777019588869 1103 86-87 0.04620552384339405 1103 88-89 0.0379118191007386 1103 90-91 0.01700187224664873 1103 92-93 -0.02607721371339622 1103 94-95 -0.15797308406096278 1103 96-97 -0.18147759199223756 1103 98-99 -0.15537220881015656 1103 100-101 -0.14672313830201134 1104 1 0.10231376756257404 1104 2 0.13739806946501432 1104 3 0.1564077263839856 1104 4 0.11025544085664052 1104 5 0.06331483400054339 1104 6 0.03241290123697382 1104 7 0.038912152955930424 1104 8 0.035506942987929335 1104 9 0.036619598494233685 1104 10-11 0.10603333112376845 1104 12-13 0.0848548841513832 1104 14-15 0.13283259445186957 1104 16-17 0.12601997554114774 1104 18-19 0.1005106398417226 1104 20-21 0.10415318029311038 1104 22-23 0.1035882520868796 1104 24-25 0.11532826076349778 1104 26-27 0.09118449401810835 1104 28-29 0.05788244131549192 1104 30-31 0.05493937722940245 1104 32-33 0.035886633965951376 1104 34-35 0.014932166377988665 1104 36-37 0.018148333191781774 1104 38-39 0.022080734898445087 1104 40-41 -0.037640328876257456 1104 42-43 -0.018879424066497563 1104 44-45 -0.05518284774593951 1104 46-47 -0.059723652855048215 1104 48-49 -0.18126530299410604 1104 50-51 -0.20220812112577136 1104 52-53 -0.1270694261334384 1104 54-55 -0.05288211953808286 1104 56-57 -0.11248792037385158 1104 58-59 -0.07066328248665599 1104 60-61 -0.15911688578083272 1104 62-63 -0.11454933501164533 1104 64-65 -0.1413971221618624 1104 66-67 -0.08008996508525712 1104 68-69 -0.08677298894147611 1104 70-71 -0.0844889684251342 1104 72-73 -0.1454739167643595 1104 74-75 -0.19800752051560266 1104 76-77 -0.183405025867728 1104 78-79 -0.1271774651094475 1104 80-81 -0.19377685210511686 1104 82-83 -0.1793840397931703 1104 84-85 -0.19693015451100848 1104 86-87 -0.2085640881270301 1104 88-89 -0.24714360428419013 1104 90-91 -0.2810319688993381 1104 92-93 -0.2608510442037719 1104 94-95 -0.2541186932002226 1104 96-97 -0.2554478510114855 1104 98-99 -0.32088609574025995 1104 100-101 -0.3990816419583325 1105 1 0.29996503049464707 1105 2 0.31650234249888953 1105 3 0.31633785728101316 1105 4 0.29848774438285375 1105 5 0.3014545196727667 1105 6 0.30493165984184856 1105 7 0.31926928785058095 1105 8 0.3284651645512682 1105 9 0.49472299842661016 1105 10-11 0.4397747038554485 1105 12-13 0.3635086411613244 1105 14-15 0.48789090819653325 1105 16-17 0.43786202859203627 1105 18-19 0.4394429290054731 1105 20-21 0.4478954039811782 1105 22-23 0.4157407299830922 1105 24-25 0.5065833779772149 1105 26-27 0.5380935457398124 1105 28-29 0.5723680722741804 1105 30-31 0.55054012752489 1105 32-33 0.493756985757166 1105 34-35 0.5936480870451675 1105 36-37 0.5593991431647467 1105 38-39 0.5039357816150556 1105 40-41 0.5455949046603124 1105 42-43 0.5373432498258381 1105 44-45 0.5830156442214403 1105 46-47 0.585971244093237 1105 48-49 0.5229832101460161 1105 50-51 0.45097551058000107 1105 52-53 0.5598134539016826 1105 54-55 0.6935180557641871 1105 56-57 0.6611727685021407 1105 58-59 0.7157784154407594 1105 60-61 0.6814342139632146 1105 62-63 0.7258127444979579 1105 64-65 0.6747192050384072 1105 66-67 0.7135944171745408 1105 68-69 0.6792560412241642 1105 70-71 0.603764251167064 1105 72-73 0.6726863732187951 1105 74-75 0.6071099648621683 1105 76-77 0.595756401009659 1105 78-79 0.5733548154210837 1105 80-81 0.5726826885530443 1105 82-83 0.5784182804512845 1105 84-85 0.6120500246499745 1105 86-87 0.49558772848875776 1105 88-89 0.5256565432299034 1105 90-91 0.5080993657468902 1105 92-93 0.5258055099084551 1105 94-95 0.5250144096816776 1105 96-97 0.467516771647432 1105 98-99 0.4903160725760678 1105 100-101 0.4214302515905075 1106 1 0.24655034053252578 1106 2 0.3164181436588578 1106 3 0.2923016237579965 1106 4 0.3188208969088535 1106 5 0.2925496583573306 1106 6 0.2979971981639977 1106 7 0.3405840040331043 1106 8 0.36487529163013477 1106 9 0.3697224146390923 1106 10-11 0.39287958612051455 1106 12-13 0.3889677149874302 1106 14-15 0.46501444364044175 1106 16-17 0.4556801911224895 1106 18-19 0.4694338619513019 1106 20-21 0.48235354574880773 1106 22-23 0.5054569959046447 1106 24-25 0.4506510292765924 1106 26-27 0.4270184626585092 1106 28-29 0.47137483286726223 1106 30-31 0.5352020278166449 1106 32-33 0.5265798552829963 1106 34-35 0.47761247800801243 1106 36-37 0.4677960092599065 1106 38-39 0.4660529420992461 1106 40-41 0.4810634325083143 1106 42-43 0.4703615714133704 1106 44-45 0.45953897937619814 1106 46-47 0.41994904548938905 1106 48-49 0.4440591405532075 1106 50-51 0.40095472774429197 1106 52-53 0.4247383988421731 1106 54-55 0.46083302681165605 1106 56-57 0.5522072266439011 1106 58-59 0.5369847806595658 1106 60-61 0.5300124077980186 1106 62-63 0.5229544207220655 1106 64-65 0.5471676699441019 1106 66-67 0.5238452589961469 1106 68-69 0.5631189865956685 1106 70-71 0.5795548162266826 1106 72-73 0.5497434236039851 1106 74-75 0.5689538460684176 1106 76-77 0.5705485559728842 1106 78-79 0.5438922602198062 1106 80-81 0.5009686283475361 1106 82-83 0.5342863124468451 1106 84-85 0.5761635382969246 1106 86-87 0.5277205278066255 1106 88-89 0.6077457086005644 1106 90-91 0.5562861550689817 1106 92-93 0.5543336714848301 1106 94-95 0.5605687042561911 1106 96-97 0.6045983878114782 1106 98-99 0.6536320991113733 1106 100-101 0.6822255836922153 1107 1 0.2099578007208045 1107 2 0.23322146878517458 1107 3 0.23865154255091525 1107 4 0.22496258114487233 1107 5 0.19327453632932645 1107 6 0.2465300984691723 1107 7 0.2449018593326926 1107 8 0.30862009395720946 1107 9 0.34099399838461153 1107 10-11 0.27423045043566674 1107 12-13 0.28919149406424083 1107 14-15 0.4070820398349042 1107 16-17 0.31657660583347536 1107 18-19 0.2995563167692765 1107 20-21 0.3315950492880404 1107 22-23 0.34064036507886897 1107 24-25 0.309744752188827 1107 26-27 0.40304310650166997 1107 28-29 0.4094030476853874 1107 30-31 0.33687233291693275 1107 32-33 0.37004248254253724 1107 34-35 0.3508969179098216 1107 36-37 0.32232734607475066 1107 38-39 0.35677990747085175 1107 40-41 0.35790722052306734 1107 42-43 0.3439277122987434 1107 44-45 0.38881452428995544 1107 46-47 0.4309130883150587 1107 48-49 0.35934033434556056 1107 50-51 0.35020854316567807 1107 52-53 0.39998939555903945 1107 54-55 0.45815096286998624 1107 56-57 0.4139496560054994 1107 58-59 0.4015833085451632 1107 60-61 0.44859965442998373 1107 62-63 0.458772528272938 1107 64-65 0.4784506673149309 1107 66-67 0.5303501201588361 1107 68-69 0.5296436132702667 1107 70-71 0.5321383672346371 1107 72-73 0.5587961786532674 1107 74-75 0.5933460064155938 1107 76-77 0.6152469985934417 1107 78-79 0.6220614819130645 1107 80-81 0.5962577266857281 1107 82-83 0.5767802216833751 1107 84-85 0.6020552484216424 1107 86-87 0.6006559160530145 1107 88-89 0.6074868465945933 1107 90-91 0.5891079360627494 1107 92-93 0.5392555307904097 1107 94-95 0.6035071869909743 1107 96-97 0.5897879722766568 1107 98-99 0.6475019895319072 1107 100-101 0.6580671403088303 1108 1 0.13733886892137903 1108 2 0.15004965377919177 1108 3 0.11311406215131825 1108 4 0.131048139417544 1108 5 0.16460562010279745 1108 6 0.15520361477778977 1108 7 0.15612225616690267 1108 8 0.15133180844283345 1108 9 0.15930976994766155 1108 10-11 0.1849670530454972 1108 12-13 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-1.3393220704765554 2316 60-61 -1.3408134403388985 2316 62-63 -1.4774302969876558 2316 64-65 -1.4029940102985705 2316 66-67 -1.2999421391837913 2316 68-69 -1.258658358323899 2316 70-71 -1.4777263978978432 2316 72-73 -1.423636009984783 2316 74-75 -1.2458391476166497 2316 76-77 -1.1882244150256156 2316 78-79 -1.1794812065981048 2316 80-81 -1.134395075207884 2316 82-83 -1.0952220855158146 2316 84-85 -1.1583485209960998 2316 86-87 -0.9992976094658879 2316 88-89 -1.0395112235467714 2316 90-91 -1.0719402212233895 2316 92-93 -1.2076490372837156 2316 94-95 -1.5244396235594806 2316 96-97 -1.3064986511303651 2316 98-99 -1.1191175300506053 2316 100-101 -1.1237094071630125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86685.0 3 18370.0 4 14592.0 5 13122.0 6 13780.0 7 14782.0 8 16220.0 9 19863.0 10 24124.0 11 29767.0 12 37557.0 13 46125.0 14 52913.0 15 64769.0 16 81368.0 17 105047.0 18 83820.0 19 49806.0 20 44104.0 21 43677.0 22 45645.0 23 50086.0 24 58400.0 25 68582.0 26 82288.0 27 99716.0 28 122622.0 29 151944.0 30 191751.0 31 247182.0 32 329101.0 33 452573.0 34 651807.0 35 997466.0 36 1585669.0 37 2389853.0 38 2428330.0 39 565423.0 40 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50113048946568 20.91459564072715 14.690395299976315 21.893878569830854 2 15.804260418512175 20.993321367405997 40.349588574578235 22.85282963950359 3 18.334893220248123 26.574171963691107 30.49158847458878 24.59934634147199 4 12.318453450974005 17.476153745494592 39.40051486835511 30.804877935176293 5 12.40114957246067 38.261951403030814 35.552355365985285 13.784543658523232 6 29.34768088766836 38.55427139776691 18.111537523210107 13.986510191354625 7 26.057074723568913 33.51394739654222 22.940686831419217 17.488291048469645 8 25.042062209623552 35.09448431181743 20.97395557193664 18.889497906622378 9 25.33317736323049 16.601798747768658 22.333541830557586 35.73148205844326 10-11 23.365451060621467 27.32414689639614 29.05833631186241 20.252065731119988 12-13 24.48071101871065 25.37245052968799 29.10437333034758 21.042465121253777 14-15 21.865150785476505 26.915761203893464 27.46193453249296 23.75715347813707 16-17 20.162845605188654 30.18722569216391 28.116084947265975 21.533843755381465 18-19 20.00377273478057 28.48503519219834 31.315025683026036 20.196166389995053 20-21 21.53329266613884 27.601906978867575 30.93875530175883 19.92604505323476 22-23 20.986135758592805 27.528352683888862 31.158240552664452 20.32727100485388 24-25 20.574780011229805 27.950427155280032 31.082349934513314 20.39244289897685 26-27 20.251196521765877 27.888007539982006 31.169253043070388 20.691542895181726 28-29 20.335317489871716 27.79324945628698 31.38718408962797 20.484248964213343 30-31 20.683716286595804 28.33328046007294 30.772887845796614 20.210115407534644 32-33 20.212204095004555 28.00682518335002 31.201662347723875 20.579308373921553 34-35 20.570050151242 27.796373223805602 31.186565898130898 20.447010726821503 36-37 20.884762108138176 28.065452886945014 30.564325843437178 20.48545916147963 38-39 20.695154885499317 28.298159891880655 30.66430318903389 20.34238203358614 40-41 20.605033156441273 27.99278751777976 30.821515275971784 20.580664049807183 42-43 20.599557972897223 28.04279206214269 30.561702388307616 20.795947576652473 44-45 20.729157544082703 28.230825040744307 30.283576158666847 20.756441256506136 46-47 21.05603107796187 28.43703823331982 29.775218709179143 20.73171197953917 48-49 21.02495534037851 28.44349695535936 29.49691212123689 21.034635583025242 50-51 21.048289717211453 28.497058570220812 29.2106738660072 21.24397784656054 52-53 21.028080239752995 28.57576719170752 29.247056777246616 21.149095791292865 54-55 21.042851161043426 29.09661097453086 29.08718093930621 20.773356925119497 56-57 21.028342568357054 28.676880888077367 29.11698157115465 21.177794972410933 58-59 20.949864806653938 28.26761853843894 29.41271384740801 21.369802807499113 60-61 21.1758983942489 28.62336679008796 29.131059848805506 21.069674966857633 62-63 20.88376791714336 28.62769905899969 29.31323810800573 21.17529491585122 64-65 21.008362523234236 28.444095601974862 29.23355210343485 21.313989771356052 66-67 20.872294183125078 28.458716142150976 29.108282239063755 21.560707435660195 68-69 21.28007277104298 28.815897952514714 28.917424841168156 20.98660443527415 70-71 21.330094159631667 28.535549322079035 28.87188111296893 21.262475405320362 72-73 21.137865083069933 28.555482362403122 28.978060040100782 21.32859251442616 74-75 21.15930892564404 28.67130670160497 28.88995817975215 21.279426192998837 76-77 21.208604216684183 28.796185512449163 28.62278852932817 21.37242174153848 78-79 21.36007845502862 28.793031535631158 28.791155230786377 21.055734778553845 80-81 21.212654937797247 28.639983842493844 28.844896949536842 21.302464270172063 82-83 21.076149707522156 28.70801302714017 29.027517218861142 21.188320046476534 84-85 20.993815540158124 28.597510924092063 28.949467944864534 21.459205590885283 86-87 21.216334388760647 28.759631889240378 28.715492802033182 21.308540919965793 88-89 20.996287459622923 28.701226907791018 28.875937016480734 21.426548616105325 90-91 20.87772397679587 28.456059359632295 28.99200742127223 21.6742092422996 92-93 20.96121066717308 28.78719167307775 28.73412638484606 21.51747127490311 94-95 20.8751767679697 28.420146047339895 29.00630564697868 21.69837153771173 96-97 20.857059058089224 28.52877328385737 28.874779890173023 21.739387767880388 98-99 20.78201808368483 28.49436208586784 28.821273661948375 21.902346168498955 100-101 20.812770248221224 28.550964621196133 28.73392858288532 21.90233654769732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5081.0 1 3280.0 2 1894.0 3 2421.5 4 2107.5 5 2020.5 6 3561.5 7 6862.0 8 10831.0 9 13003.0 10 11879.5 11 10269.5 12 10569.0 13 11519.0 14 12401.0 15 13328.5 16 14286.5 17 15540.5 18 17379.5 19 19561.0 20 22662.5 21 27162.5 22 34214.0 23 44881.5 24 57000.5 25 71788.0 26 91525.5 27 116389.5 28 148250.5 29 180262.5 30 208297.5 31 239460.0 32 274239.5 33 304837.0 34 332364.5 35 362122.5 36 385473.5 37 400865.5 38 413719.5 39 420758.0 40 425664.5 41 424694.0 42 421467.5 43 417110.5 44 406342.5 45 396994.5 46 389227.5 47 378068.5 48 365488.0 49 357336.0 50 350499.0 51 331474.5 52 301818.0 53 272753.0 54 251285.5 55 241144.5 56 221385.5 57 193185.5 58 170197.0 59 149100.5 60 126040.5 61 101632.5 62 81962.0 63 66365.5 64 53144.0 65 41794.5 66 32447.5 67 24645.0 68 17719.0 69 12615.5 70 9275.0 71 6591.0 72 4625.0 73 3274.5 74 2372.0 75 1769.0 76 1233.5 77 932.5 78 750.0 79 601.0 80 506.5 81 416.5 82 310.5 83 267.0 84 254.0 85 201.0 86 163.5 87 140.0 88 125.5 89 102.5 90 79.0 91 77.5 92 66.0 93 52.0 94 45.0 95 33.5 96 25.5 97 20.0 98 16.5 99 13.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014622813575208496 2 0.0044202375170251645 3 0.003945699095714312 4 0.005694461055730232 5 0.0025660225744957217 6 0.007162015062376757 7 0.0013269500299618287 8 0.004393874271396783 9 0.010808930707636088 10-11 0.007205953805090725 12-13 0.008682295560280044 14-15 0.003506311668574633 16-17 0.006375511567796733 18-19 0.004090696946670406 20-21 0.0010413482023210376 22-23 0.0011687705561915444 24-25 0.007293831290518661 26-27 0.0012434664188052899 28-29 7.425647518660564E-4 30-31 5.799914038243755E-4 32-33 0.0013269500299618287 34-35 0.0017531558342873165 36-37 0.0013093745328762415 38-39 0.0017751252056443004 40-41 0.002790110162336958 42-43 0.0027461714196229895 44-45 0.00786942882007164 46-47 0.01442508923299564 48-49 0.005553857079045535 50-51 0.005791126289700961 52-53 0.014082367039826692 54-55 0.008282453001582937 56-57 0.005320981742661505 58-59 0.006814898994936412 60-61 0.0014280091382039546 62-63 0.002271632998312137 64-65 0.0022672391240407402 66-67 0.0023507227351972793 68-69 0.0019992127934855366 70-71 0.002104665775999059 72-73 0.0013269500299618287 74-75 0.001603764109059826 76-77 0.008480177343795792 78-79 0.006423844184782097 80-81 0.013054200460319845 82-83 0.014886446031492303 84-85 0.00842745085253903 86-87 0.005936124140657055 88-89 0.0030185916244495903 90-91 0.002618749065752483 92-93 8.831687285507535E-4 94-95 0.0013621010241330028 96-97 0.0019025475595148074 98-99 0.006274452459554608 100-101 0.011265893631861354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1379479E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.43546754461929 #Duplication Level Percentage of deduplicated Percentage of total 1 65.99264392266662 31.3039191897727 2 17.586355180458124 16.684339607815375 3 7.224488423719913 10.280909584495312 4 3.442520784447672 6.531903317693796 5 1.879972428144013 4.4588685550002225 6 1.0894065774619013 3.100590620885328 7 0.6828840735124699 2.267504771408673 8 0.4489775447224698 1.703796780075646 9 0.32626474299492153 1.392886856456024 >10 1.1884828047522227 9.292451609760617 >50 0.06843925586286015 2.2555980844922106 >100 0.06071549857519631 5.89289987069523 >500 0.006468037939158054 2.1107475988080164 >1k 0.0022877286653666244 1.7004590428226936 >5k 1.8599215473529985E-5 0.05036836509452622 >10k+ 7.439686189411994E-5 0.9727561447236254 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42904 0.3770295634800152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36391 0.3197949572208007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20579 0.18084307726214882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.787748542793568E-6 0.0 9 0.0 0.0 0.0 1.7575497085587136E-5 0.0 10-11 0.0 0.0 0.0 2.63632456283807E-5 0.0 12-13 0.0 0.0 0.0 2.63632456283807E-5 0.0 14-15 0.0 0.0 0.0 4.3938742713967834E-5 0.0 16-17 0.0 0.0 0.0 8.787748542793567E-5 0.0 18-19 0.0 8.787748542793568E-6 0.0 1.0105910824212602E-4 0.0 20-21 0.0 8.787748542793568E-6 0.0 1.318162281419035E-4 0.0 22-23 0.0 1.7575497085587136E-5 0.0 2.460569591982199E-4 0.0 24-25 0.0 3.075711989977749E-5 0.0 4.7014454703945587E-4 0.0 26-27 0.0 3.515099417117427E-5 0.0 8.084728659370083E-4 0.0 28-29 0.0 3.515099417117427E-5 0.0 0.0017355803372017295 0.0 30-31 0.0 3.515099417117427E-5 0.0 0.004389480397125387 0.0 32-33 0.0 3.515099417117427E-5 0.0 0.01042226977175317 0.0 34-35 0.0 3.515099417117427E-5 0.0 0.021371804456073958 0.0 36-37 0.0 3.515099417117427E-5 0.0 0.03896487703874668 0.0 38-39 0.0 3.515099417117427E-5 0.0 0.06294664281203033 0.0 40-41 0.0 3.515099417117427E-5 0.0 0.08848823395165983 0.0 42-43 0.0 4.3938742713967834E-5 0.0 0.11362558865832083 0.0 44-45 0.0 4.3938742713967834E-5 0.0 0.14566132597107478 0.0 46-47 0.0 5.27264912567614E-5 0.0 0.18477120086077753 0.0 48-49 0.0 5.27264912567614E-5 0.0 0.22574847231582396 0.0 50-51 0.0 5.27264912567614E-5 0.0 0.267995573435304 0.0 52-53 0.0 5.27264912567614E-5 0.0 0.31022509905769846 0.0 54-55 0.0 5.27264912567614E-5 0.0 0.3574416719781284 0.0 56-57 0.0 7.469586261374533E-5 0.0 0.41492672907081246 0.0 58-59 0.0 9.666523397072924E-5 0.0 0.4805316658170379 0.0 60-61 0.0 9.666523397072924E-5 0.0 0.5533864951110679 0.0 62-63 0.0 1.0105910824212602E-4 0.0 0.6266323792152523 0.0 64-65 0.0 1.054529825135228E-4 0.0 0.7042765314651048 0.0 66-67 0.0 1.0984685678491959E-4 0.0 0.7969565214716772 0.0 68-69 0.0 1.1424073105631637E-4 8.787748542793568E-6 0.9052743100101507 0.0 70-71 0.0 1.1424073105631637E-4 8.787748542793568E-6 1.0229993833636848 0.0 72-73 0.0 1.1424073105631637E-4 8.787748542793568E-6 1.147139513153458 0.0 74-75 0.0 1.2302847959910995E-4 8.787748542793568E-6 1.2758668476825696 0.0 76-77 0.0 1.3181622814190352E-4 8.787748542793568E-6 1.4193136610208605 0.0 78-79 0.0 1.3181622814190352E-4 8.787748542793568E-6 1.588117522779382 0.0 80-81 0.0 1.362101024133003E-4 8.787748542793568E-6 1.778253644125535 0.0 82-83 0.0 1.406039766846971E-4 8.787748542793568E-6 1.977480691339208 0.0 84-85 0.0 1.406039766846971E-4 8.787748542793568E-6 2.179396789606976 0.0 86-87 0.0 1.406039766846971E-4 8.787748542793568E-6 2.401854250093524 0.0 88-89 0.0 1.406039766846971E-4 8.787748542793568E-6 2.6529641647038495 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22865 0.0 59.311543 1 GTATCAA 43860 0.0 43.17993 1 ATCAACG 54265 0.0 34.27787 3 TCAACGC 54985 0.0 33.7958 4 TATCAAC 56895 0.0 32.875008 2 CAACGCA 57865 0.0 32.13767 5 TGGTATC 9115 0.0 31.473461 2 AACGCAG 60630 0.0 30.695957 6 GTGGTAT 9650 0.0 30.321991 1 CGCAGAG 66645 0.0 27.335253 8 ACGCAGA 67390 0.0 27.181091 7 GTACATG 47140 0.0 26.168974 1 TACATGG 46110 0.0 25.556177 2 GCAGAGT 72100 0.0 24.509417 9 ACATGGG 47525 0.0 24.067184 3 GAGTACT 45305 0.0 21.835073 12-13 CATGGGT 11355 0.0 21.209429 4 CAGAGTA 71040 0.0 20.999489 10-11 AGAGTAC 66965 0.0 20.66003 10-11 GTACTTT 48905 0.0 19.983204 14-15 >>END_MODULE