FastQCFastQC Report
Wed 25 May 2016
SRR2049423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11379479
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424720.3732332561095284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364100.3199619244431138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237360.20858599941174813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225150.058.6312181
GTATCAA425700.042.9536481
ATCAACG556050.032.5198633
TCAACGC563450.032.0586244
TGGTATC89900.032.0184482
GTGGTAT95300.031.5132241
TATCAAC577600.031.3564532
CAACGCA585250.030.9131685
AACGCAG616750.029.4951236
CGCAGAG697850.025.6793168
ACGCAGA704500.025.598617
GTACATG450900.024.3971671
TACATGG444350.023.730952
GCAGAGT753250.023.3997149
ACATGGG456350.022.2738553
AGAGTAC706200.020.98050710-11
GAGTACT486250.020.61993612-13
AGTACTT499250.020.22095712-13
CAGAGTA744900.019.9415110-11
CATGGGT116200.019.4569514
Produced by FastQC (version 0.11.5)