##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049423_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11379479 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.237079746796844 33.0 31.0 34.0 31.0 34.0 2 32.4138787021796 34.0 31.0 34.0 31.0 34.0 3 32.486416117996264 34.0 31.0 34.0 31.0 34.0 4 36.09469194503544 37.0 35.0 37.0 35.0 37.0 5 36.00824949894455 37.0 35.0 37.0 35.0 37.0 6 36.053320543058256 37.0 35.0 37.0 35.0 37.0 7 35.98776736615095 37.0 35.0 37.0 35.0 37.0 8 35.98019918135092 37.0 35.0 37.0 35.0 37.0 9 37.62847675187941 39.0 37.0 39.0 35.0 39.0 10-11 37.62288673321511 39.0 37.0 39.0 35.0 39.0 12-13 37.61413646441986 39.0 37.0 39.0 35.0 39.0 14-15 38.886318960648374 40.0 38.0 41.0 35.5 41.0 16-17 38.874478963404215 40.0 38.0 41.0 35.0 41.0 18-19 38.915205915842016 40.0 38.0 41.0 35.0 41.0 20-21 38.91408745514623 40.0 38.0 41.0 35.0 41.0 22-23 38.83388351962335 40.0 38.0 41.0 35.0 41.0 24-25 38.746319800757135 40.0 38.0 41.0 35.0 41.0 26-27 38.594521067265035 40.0 38.0 41.0 34.5 41.0 28-29 38.429142054746094 40.0 38.0 41.0 34.0 41.0 30-31 38.25161310109189 40.0 38.0 41.0 34.0 41.0 32-33 38.008096679997394 40.0 37.0 41.0 34.0 41.0 34-35 37.84167232085054 40.0 37.0 41.0 33.0 41.0 36-37 37.66124174050499 40.0 37.0 41.0 33.0 41.0 38-39 37.806284716549854 40.0 37.0 41.0 33.0 41.0 40-41 37.71786256646723 40.0 37.0 41.0 33.0 41.0 42-43 37.53001547786151 40.0 37.0 41.0 33.0 41.0 44-45 37.287855533632076 40.0 37.0 41.0 32.5 41.0 46-47 36.94936701407859 40.0 37.0 41.0 31.5 41.0 48-49 36.66293188818223 40.0 36.5 41.0 31.0 41.0 50-51 36.440161627786296 39.5 36.0 41.0 30.5 41.0 52-53 36.265835369088514 39.0 36.0 41.0 30.0 41.0 54-55 36.167847403207126 39.0 36.0 41.0 30.0 41.0 56-57 36.08388512338746 39.0 35.5 41.0 30.0 41.0 58-59 35.85496488899008 39.0 35.0 40.5 29.0 41.0 60-61 35.62022698930241 39.0 35.0 40.0 29.0 41.0 62-63 35.35361781501596 38.0 35.0 40.0 28.0 41.0 64-65 35.10476547300628 38.0 35.0 40.0 28.0 41.0 66-67 34.775893474560654 37.5 34.0 40.0 28.0 41.0 68-69 34.460870660247274 37.0 34.0 40.0 27.0 41.0 70-71 34.12771880856759 36.5 34.0 39.0 26.5 41.0 72-73 33.76103141453137 36.0 34.0 39.0 26.0 40.5 74-75 33.387079144836065 35.5 33.5 38.5 26.0 40.0 76-77 31.376193321328685 33.5 30.5 36.0 23.5 38.5 78-79 32.67779487092511 35.0 33.0 37.0 26.0 39.0 80-81 32.67563611655683 35.0 33.0 37.0 26.0 39.0 82-83 32.400212654726985 35.0 33.0 36.0 26.0 38.5 84-85 32.136029470242 35.0 33.0 36.0 25.0 37.0 86-87 31.877986065970155 35.0 33.0 36.0 25.0 37.0 88-89 31.619954876668782 35.0 33.0 35.0 24.5 37.0 90-91 31.36336514176088 35.0 33.0 35.0 24.0 36.0 92-93 31.141196270936483 35.0 32.0 35.0 23.0 36.0 94-95 30.907188808907684 35.0 32.0 35.0 20.0 36.0 96-97 30.696238949076665 35.0 32.0 35.0 19.0 35.5 98-99 30.442294370418892 34.0 32.0 35.0 14.0 35.0 100-101 29.86657662446585 34.0 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3967337156681907 1101 2 0.4070629390753382 1101 3 0.37942733184428334 1101 4 0.2904725305494864 1101 5 0.3171170083226258 1101 6 0.30191669822980316 1101 7 0.2996923797524218 1101 8 0.31692746196456056 1101 9 0.37934227712543844 1101 10-11 0.3479774834354856 1101 12-13 0.3171912611788983 1101 14-15 0.4099210279012979 1101 16-17 0.38008253016003124 1101 18-19 0.36625053176095435 1101 20-21 0.3129790805638919 1101 22-23 0.3131686386439654 1101 24-25 0.29323950450412894 1101 26-27 0.29154664928501717 1101 28-29 0.31892614138326536 1101 30-31 0.29592726132086966 1101 32-33 0.30290907107197285 1101 34-35 0.2520052297163602 1101 36-37 0.19858699286212556 1101 38-39 0.30486293304214485 1101 40-41 0.36169938977791816 1101 42-43 0.3544845849091587 1101 44-45 0.31803165540522826 1101 46-47 0.3361026484796241 1101 48-49 0.1905994723692146 1101 50-51 0.21436674778259857 1101 52-53 0.26260882069960445 1101 54-55 0.21777838931387095 1101 56-57 0.1414886992494928 1101 58-59 0.15690323760640723 1101 60-61 0.1960559010790206 1101 62-63 0.2079568321948173 1101 64-65 0.08427049854572743 1101 66-67 0.04621268780879717 1101 68-69 -0.031161074035317426 1101 70-71 -0.022496419205459972 1101 72-73 -0.0336161097791603 1101 74-75 -0.08072171573333975 1101 76-77 -0.11260734631323288 1101 78-79 -0.04145669868443491 1101 80-81 -0.09625333915288792 1101 82-83 -0.13043603482682187 1101 84-85 -0.14626127628251595 1101 86-87 -0.14986831837707015 1101 88-89 -0.14425057840168165 1101 90-91 -0.18792546513876474 1101 92-93 -0.22483369616501037 1101 94-95 -0.21173925466448296 1101 96-97 -0.25887828561769766 1101 98-99 -0.31096788751865034 1101 100-101 -0.3303301870693822 1102 1 0.33468025575245974 1102 2 0.336320990958086 1102 3 0.2969193383655977 1102 4 0.24173403932257287 1102 5 0.2616592252420631 1102 6 0.24703566495291795 1102 7 0.25804373019544613 1102 8 0.27579931093998766 1102 9 0.4102544296651729 1102 10-11 0.33145967364659157 1102 12-13 0.2832283239351554 1102 14-15 0.36535222879123097 1102 16-17 0.37411233810151856 1102 18-19 0.32389187700137256 1102 20-21 0.2854506564496191 1102 22-23 0.3202772813110357 1102 24-25 0.30027140547840503 1102 26-27 0.2454982014485907 1102 28-29 0.27798583630832496 1102 30-31 0.24796611444052985 1102 32-33 0.2885735424327933 1102 34-35 0.17871016359499237 1102 36-37 0.1754738301810974 1102 38-39 0.2805082115577875 1102 40-41 0.25730880296193703 1102 42-43 0.29946233707621417 1102 44-45 0.25382214142326376 1102 46-47 0.2992863718564962 1102 48-49 0.1428840055200027 1102 50-51 0.15996417835508936 1102 52-53 0.19749461585696082 1102 54-55 0.2379383069460701 1102 56-57 0.16893310320424604 1102 58-59 0.1789552162413912 1102 60-61 0.16900273828876067 1102 62-63 0.10719482962593929 1102 64-65 0.11383752892687227 1102 66-67 0.1040590996460864 1102 68-69 0.10603686323982942 1102 70-71 0.01918495679823451 1102 72-73 -0.023698602239775823 1102 74-75 0.0012337170544611809 1102 76-77 -0.011326296804750058 1102 78-79 0.09694353467920536 1102 80-81 0.07397074544193316 1102 82-83 0.15904567781846168 1102 84-85 0.08048897189474502 1102 86-87 0.03710686419188747 1102 88-89 0.019118761048638078 1102 90-91 -0.040300211911681316 1102 92-93 -0.019670699597874375 1102 94-95 -0.029602513835417454 1102 96-97 -0.13066433819248502 1102 98-99 -0.17864198774557138 1102 100-101 -0.15163691960107428 1103 1 0.33847852372466036 1103 2 0.3394166194397812 1103 3 0.30389613870264043 1103 4 0.24146060112625634 1103 5 0.23560311952826396 1103 6 0.24521578795938836 1103 7 0.2407810007474751 1103 8 0.2629988822165643 1103 9 0.34722220174733565 1103 10-11 0.2959593946123107 1103 12-13 0.28958734572466227 1103 14-15 0.36461386706438503 1103 16-17 0.3801003202137636 1103 18-19 0.33154658242236934 1103 20-21 0.29584493908093634 1103 22-23 0.23637018100686902 1103 24-25 0.27201521870932055 1103 26-27 0.24828466163796037 1103 28-29 0.2733225793376235 1103 30-31 0.26062289968950836 1103 32-33 0.2677429278930603 1103 34-35 0.25918620794105607 1103 36-37 0.2591633745992681 1103 38-39 0.31163890262877203 1103 40-41 0.3578876949667844 1103 42-43 0.3950409648386852 1103 44-45 0.387239074483233 1103 46-47 0.41146440368561343 1103 48-49 0.32807603270040175 1103 50-51 0.3847676620410212 1103 52-53 0.30539781600029414 1103 54-55 0.3075396694243864 1103 56-57 0.30004481629682545 1103 58-59 0.30295904528785655 1103 60-61 0.2555744737738763 1103 62-63 0.4174546347392507 1103 64-65 0.34716430676480314 1103 66-67 0.32301551738595435 1103 68-69 0.2546902771899866 1103 70-71 0.23384115609250244 1103 72-73 0.2660531180786734 1103 74-75 0.2482357843698324 1103 76-77 0.2556939851571087 1103 78-79 0.3104694258259926 1103 80-81 0.2768701476316835 1103 82-83 0.35451678047059687 1103 84-85 0.2914485681512602 1103 86-87 0.288801473638042 1103 88-89 0.26197871520030347 1103 90-91 0.31407315970058747 1103 92-93 0.22694757964258727 1103 94-95 0.18909319052675855 1103 96-97 0.20313994966425852 1103 98-99 0.18416022826862033 1103 100-101 0.13898925276813756 1104 1 0.3068037856036838 1104 2 0.2874506589198589 1104 3 0.29032054699325727 1104 4 0.22314108094223428 1104 5 0.1973787056135663 1104 6 0.251400175410474 1104 7 0.2731574571750386 1104 8 0.24868060621764698 1104 9 0.3124723166388961 1104 10-11 0.24626803012927212 1104 12-13 0.2372511231351453 1104 14-15 0.30258329713938537 1104 16-17 0.32550621776861277 1104 18-19 0.2657595571648059 1104 20-21 0.2895196344908939 1104 22-23 0.3005290602033952 1104 24-25 0.2639471417171322 1104 26-27 0.26254868403869835 1104 28-29 0.28846072848606497 1104 30-31 0.2657120486331692 1104 32-33 0.2634158238747233 1104 34-35 0.25722195862920216 1104 36-37 0.24454926946938116 1104 38-39 0.3166741323747573 1104 40-41 0.3585390840764475 1104 42-43 0.3707789056379269 1104 44-45 0.36274404191423315 1104 46-47 0.43770817789783933 1104 48-49 0.37863529331952606 1104 50-51 0.3574707837842226 1104 52-53 0.2535153441382576 1104 54-55 0.3012309157372002 1104 56-57 0.30728420657232647 1104 58-59 0.36237271990670195 1104 60-61 0.321522791538726 1104 62-63 0.3284936587547236 1104 64-65 0.37898704209047196 1104 66-67 0.35314171598304256 1104 68-69 0.29717749029146034 1104 70-71 0.24554451628247165 1104 72-73 0.19140827716114472 1104 74-75 0.19430058928004001 1104 76-77 0.19414696604082593 1104 78-79 0.3003636198169417 1104 80-81 0.2801878156981843 1104 82-83 0.26067698961251295 1104 84-85 0.24137806653251914 1104 86-87 0.17692565350160194 1104 88-89 0.1959121195946416 1104 90-91 0.1794090141113145 1104 92-93 0.14639119901893238 1104 94-95 0.07159413388209401 1104 96-97 0.06327807874866309 1104 98-99 0.05770193267705892 1104 100-101 0.10090150487801353 1105 1 0.29604523872844624 1105 2 0.29847836431388686 1105 3 0.2652159844618467 1105 4 0.19683087320133552 1105 5 0.20858893759417185 1105 6 0.24133592801545944 1105 7 0.2349945922150738 1105 8 0.24979802324911304 1105 9 0.3072280496250386 1105 10-11 0.26331146139229133 1105 12-13 0.2703490514291573 1105 14-15 0.3448447555332663 1105 16-17 0.30758886440711564 1105 18-19 0.33125187777984166 1105 20-21 0.31893394454979784 1105 22-23 0.24182797113284238 1105 24-25 0.29937082638529233 1105 26-27 0.31833646338312604 1105 28-29 0.32952832616122407 1105 30-31 0.33403852241863063 1105 32-33 0.31328993591147025 1105 34-35 0.3394830700457021 1105 36-37 0.2896930681724399 1105 38-39 0.3495856821087244 1105 40-41 0.35639469644861066 1105 42-43 0.41479869249115353 1105 44-45 0.42281358202570374 1105 46-47 0.39824501334951634 1105 48-49 0.3956711367943626 1105 50-51 0.37563642831024424 1105 52-53 0.3439149447920258 1105 54-55 0.3392668903964662 1105 56-57 0.35994210010715477 1105 58-59 0.370735253109828 1105 60-61 0.3766659011582121 1105 62-63 0.4423744661808229 1105 64-65 0.4741264211411078 1105 66-67 0.4944357557398291 1105 68-69 0.39159395734309044 1105 70-71 0.36949265867764325 1105 72-73 0.3663131084988436 1105 74-75 0.3956307791332492 1105 76-77 0.4387119944909088 1105 78-79 0.5076385138417692 1105 80-81 0.4837541810603412 1105 82-83 0.50955514168745 1105 84-85 0.4826832987452576 1105 86-87 0.442077591559471 1105 88-89 0.46875922896022715 1105 90-91 0.4704343955812753 1105 92-93 0.4946655729898737 1105 94-95 0.5095759560896127 1105 96-97 0.5166424984070837 1105 98-99 0.4938447298295543 1105 100-101 0.6162171986459732 1106 1 0.3094990391184851 1106 2 0.26202540391069107 1106 3 0.25808843923165625 1106 4 0.19441806270143047 1106 5 0.24202174832091572 1106 6 0.2326158771492146 1106 7 0.23676163363996494 1106 8 0.2349672182161342 1106 9 0.28931644773209797 1106 10-11 0.2464366994941969 1106 12-13 0.23070650953156502 1106 14-15 0.34393945120650216 1106 16-17 0.3655277742122749 1106 18-19 0.31138895776140174 1106 20-21 0.3197935800128562 1106 22-23 0.3168589118714138 1106 24-25 0.3158637578859995 1106 26-27 0.3154605592630517 1106 28-29 0.378858505040391 1106 30-31 0.35909998690466693 1106 32-33 0.35778948989780446 1106 34-35 0.3402005282281664 1106 36-37 0.39559562702989837 1106 38-39 0.4312033139596423 1106 40-41 0.4304314303609331 1106 42-43 0.4109773194629369 1106 44-45 0.4101292135870622 1106 46-47 0.43792191531257174 1106 48-49 0.41645717499875445 1106 50-51 0.4566150296621032 1106 52-53 0.45577250330588726 1106 54-55 0.42315201969372396 1106 56-57 0.3552864113146015 1106 58-59 0.3887738204485558 1106 60-61 0.4335570958410031 1106 62-63 0.45242648665275453 1106 64-65 0.49820415361697457 1106 66-67 0.4227529865453974 1106 68-69 0.46087025430212236 1106 70-71 0.4215709422489269 1106 72-73 0.432497266440393 1106 74-75 0.39006491555805667 1106 76-77 0.39297484432140806 1106 78-79 0.3784863334454158 1106 80-81 0.403748469950429 1106 82-83 0.3796823673356684 1106 84-85 0.3826006344324213 1106 86-87 0.3971714545849103 1106 88-89 0.4010075848477719 1106 90-91 0.45301150724081296 1106 92-93 0.4058672545648072 1106 94-95 0.5346439589958756 1106 96-97 0.509278902863084 1106 98-99 0.6003009861302857 1106 100-101 0.617356218136468 1107 1 0.24506583568415863 1107 2 0.24369372071851814 1107 3 0.21638758381684653 1107 4 0.19639997683399457 1107 5 0.19188331365524647 1107 6 0.1419229907642361 1107 7 0.19017972260503058 1107 8 0.1805904370672664 1107 9 0.2135234986664969 1107 10-11 0.16928693711037823 1107 12-13 0.21213661523112393 1107 14-15 0.21605610000455755 1107 16-17 0.22190235738484887 1107 18-19 0.17528679954380522 1107 20-21 0.18205471301692455 1107 22-23 0.12873502218787536 1107 24-25 0.1814916379193292 1107 26-27 0.18033856787467784 1107 28-29 0.22690733343015523 1107 30-31 0.22922564389889288 1107 32-33 0.22587583141551448 1107 34-35 0.21724465068736265 1107 36-37 0.23803796193652005 1107 38-39 0.22845797017575364 1107 40-41 0.20589685819111736 1107 42-43 0.16065664062502805 1107 44-45 0.11704341086613823 1107 46-47 0.14814988091556103 1107 48-49 0.09979149010779054 1107 50-51 0.15401710382739964 1107 52-53 0.18390219797964136 1107 54-55 0.2269333535160385 1107 56-57 0.25620361149736226 1107 58-59 0.32048084410345723 1107 60-61 0.31071177711420717 1107 62-63 0.26590634153757264 1107 64-65 0.32127399079136865 1107 66-67 0.3393780189143243 1107 68-69 0.3153843939531882 1107 70-71 0.3665973194088892 1107 72-73 0.37924821880216086 1107 74-75 0.3258965055423175 1107 76-77 0.35784523255156486 1107 78-79 0.464526764928479 1107 80-81 0.4775962088508905 1107 82-83 0.4769990720627746 1107 84-85 0.4550331790401856 1107 86-87 0.43105074674135224 1107 88-89 0.40616738468336777 1107 90-91 0.4632753466279702 1107 92-93 0.45055936891511905 1107 94-95 0.5021078940407371 1107 96-97 0.4873224937332772 1107 98-99 0.5454677364802833 1107 100-101 0.6193587116608619 1108 1 0.13721323783014583 1108 2 0.13420791400247367 1108 3 0.1040591167161864 1108 4 0.14096181244619999 1108 5 0.08559362180457697 1108 6 0.09860333184862213 1108 7 0.15937106565792192 1108 8 0.1390049448324433 1108 9 0.1920437458251243 1108 10-11 0.08633458792779436 1108 12-13 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2316 56-57 -1.0946534077061756 2316 58-59 -0.8129530143655614 2316 60-61 -0.6975612609402404 2316 62-63 -1.264276686386168 2316 64-65 -1.5289423654672802 2316 66-67 -1.0165600349589354 2316 68-69 -1.064695305753098 2316 70-71 -0.9207984988164881 2316 72-73 -1.0261981793094748 2316 74-75 -0.8187562387191107 2316 76-77 -0.7987098509754915 2316 78-79 -0.8094479148783265 2316 80-81 -0.7148910164709932 2316 82-83 -0.7862648650236466 2316 84-85 -0.9259577680641229 2316 86-87 -1.0956064857285703 2316 88-89 -0.8614512679581914 2316 90-91 -0.7723044535932182 2316 92-93 -0.7192431200029787 2316 94-95 -1.015473686905576 2316 96-97 -0.9667043739249372 2316 98-99 -1.0564450578364735 2316 100-101 -1.3474329260190068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 63.0 5 127.0 6 302.0 7 861.0 8 2351.0 9 5387.0 10 9433.0 11 15215.0 12 23503.0 13 34004.0 14 43949.0 15 57684.0 16 75485.0 17 103296.0 18 86652.0 19 46736.0 20 34503.0 21 32537.0 22 32846.0 23 35251.0 24 40706.0 25 49274.0 26 62050.0 27 78912.0 28 101332.0 29 130781.0 30 171477.0 31 224896.0 32 303068.0 33 421626.0 34 615804.0 35 960103.0 36 1583219.0 37 2571569.0 38 2773962.0 39 650047.0 40 444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.31854632171298 22.301115707703605 15.859759246556616 22.520578724026798 2 15.039441634790576 21.92437811417462 39.56738155755681 23.468798693477996 3 17.279575824693048 27.02441753315583 31.202503179856034 24.493503462295088 4 12.171488001841917 18.313003566515267 38.92310308358353 30.592405348059287 5 12.220563149492042 38.34389311497901 35.82884392119257 13.606699814336373 6 29.41391205205171 39.061984398160206 17.79597319659146 13.728130353196624 7 25.992648696834014 33.48791275945059 22.919247884723017 17.600190658992386 8 25.13989657522076 35.3837232252657 20.729966699702747 18.7464134998108 9 25.077356448655543 16.534705278323717 22.292910958467733 36.095027314553015 10-11 23.37284247534772 27.544392202242 29.018322109032084 20.06444321337819 12-13 24.50637893935029 25.426109176536922 29.15185564750861 20.915656236604175 14-15 21.725011695457024 27.01181523467126 27.54899118966404 23.714181880207676 16-17 20.101539798087416 30.18676426223028 28.269914641962078 21.441781297720222 18-19 19.935895103763144 28.36828398219461 31.65336552462794 20.04245538941431 20-21 21.42175994509385 27.493533829829165 31.26627966677556 19.818426558301425 22-23 20.84064657090188 27.433378101053663 31.484995929954263 20.240979398090193 24-25 20.545710574246346 27.764068797288292 31.40204555564519 20.288175072820174 26-27 20.23668836142268 27.73911418006012 31.434887653024752 20.589309805492455 28-29 20.317941391579556 27.657576416439294 31.739589571265032 20.284892620716118 30-31 20.66848736274202 28.121603633248743 31.134816824954154 20.075092179055083 32-33 20.16831939291614 27.831506949658547 31.547209003961736 20.452964653463575 34-35 20.49263122833514 27.690415137021564 31.46020476609915 20.356748868544155 36-37 20.82411254706111 27.921262193177572 30.85558248294406 20.399042776817257 38-39 20.637154874077044 28.092459210593386 31.038031738666582 20.23235417666299 40-41 20.474476493852485 27.90191714101017 31.144621660013026 20.478984705124315 42-43 20.474484540680255 27.883905510321927 30.908946468404036 20.732663480593782 44-45 20.589201953847798 28.083332571722636 30.671168547493576 20.65629692693599 46-47 20.887138300102478 28.34304802898604 30.06792663538473 20.701887035526756 48-49 20.877157113808625 28.294740460884093 29.81081730556926 21.017285119738023 50-51 20.9563655404556 28.378926492139843 29.453973108499753 21.2107348589048 52-53 20.958606319274672 28.453015660348058 29.45282241385174 21.13555560652553 54-55 21.034243972098643 28.937199978030424 29.289785247432302 20.738770802438623 56-57 21.0398446778994 28.512367843453234 29.294738730551067 21.153048748096296 58-59 20.900755862299267 28.184895716047986 29.571714646328722 21.342633775324025 60-61 21.10781561104424 28.528016447405424 29.298623863853674 21.06554407769666 62-63 20.838401797808736 28.484739593851437 29.45824508009624 21.218613528243583 64-65 20.857106651221148 28.38293368850288 29.425097417656577 21.334862242619394 66-67 20.79097110709274 28.369537056826843 29.32331111198603 21.51618072409439 68-69 21.18115890572088 28.65190925136985 29.088571208609572 21.0783606342997 70-71 21.256468387726787 28.42165012125645 29.015387565439642 21.306493925577115 72-73 21.029712527249877 28.468947728304695 29.105799782747944 21.395539961697484 74-75 21.080845324310015 28.559047489821705 29.0029265904053 21.357180595462985 76-77 21.060761094072564 28.713321384991218 28.8001943183757 21.42572320256052 78-79 21.206556033210777 28.701627302732586 28.9171741277373 21.174642536319343 80-81 20.985995753103897 28.597177550127007 28.961789139547673 21.455037557221427 82-83 20.914629476502864 28.59766067117154 29.155503461697503 21.332206390628098 84-85 20.81777104827294 28.57280135191573 29.04585761604899 21.563569983762335 86-87 20.960015417995624 28.710915306394437 28.87433164737821 21.454737628231733 88-89 20.787789014543996 28.628897018660464 28.955520324494856 21.627793642300684 90-91 20.646952681672996 28.42341901761729 29.07064939570711 21.858978905002605 92-93 20.719227949712227 28.731620622619726 28.829133985724813 21.720017441943234 94-95 20.603165350893786 28.380876780904778 29.047065480017086 21.96889238818435 96-97 20.561372868722387 28.540700853345886 28.930118014574003 21.96780826335773 98-99 20.375345569413238 28.516846264484606 28.917640435920784 22.190167730181372 100-101 20.35448814084442 28.640461363869242 28.782620784538622 22.222429710747715 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4800.0 1 3209.5 2 1907.0 3 2276.5 4 1978.5 5 1888.0 6 3472.5 7 7348.0 8 11483.5 9 13484.0 10 12336.0 11 10542.0 12 10725.5 13 11600.0 14 12642.5 15 13681.5 16 14699.0 17 15947.0 18 17655.5 19 19879.0 20 23001.0 21 27337.0 22 34193.5 23 44649.5 24 57047.5 25 71810.0 26 91393.5 27 116302.0 28 148327.0 29 181322.5 30 210838.5 31 243028.0 32 277142.0 33 307541.0 34 336412.5 35 366014.0 36 388012.5 37 402753.0 38 416480.0 39 424416.5 40 428379.5 41 427285.0 42 424359.0 43 420036.5 44 410071.5 45 401123.0 46 391601.0 47 381347.5 48 369136.0 49 359818.0 50 352754.5 51 332835.0 52 299202.0 53 268917.5 54 250159.5 55 237289.0 56 216430.5 57 189293.5 58 166473.5 59 145628.0 60 121827.0 61 97278.0 62 78347.0 63 62816.5 64 49564.5 65 38575.5 66 29561.0 67 21972.0 68 15528.5 69 10883.0 70 7753.5 71 5229.0 72 3469.0 73 2343.0 74 1642.5 75 1133.0 76 677.0 77 446.5 78 334.0 79 227.0 80 154.5 81 113.0 82 80.5 83 60.5 84 38.5 85 30.0 86 28.0 87 16.0 88 9.5 89 7.5 90 4.0 91 4.5 92 4.0 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04704081794957397 2 0.0022848146211263275 3 2.8999570191218775E-4 4 2.109059650270456E-4 5 0.002618749065752483 6 0.001581794737702842 7 0.0 8 8.787748542793566E-6 9 1.2302847959910993E-4 10-11 2.63632456283807E-5 12-13 8.787748542793566E-6 14-15 5.316587868390108E-4 16-17 0.0 18-19 4.393874271396784E-5 20-21 5.404465353818044E-4 22-23 0.0 24-25 6.766566377951047E-4 26-27 0.007434435267203358 28-29 0.00739049652448939 30-31 0.001155588933377354 32-33 0.006630356275537746 34-35 0.007790339083186498 36-37 0.004600386362152433 38-39 0.002627536814295277 40-41 0.002135422895898837 42-43 0.001423615263932558 44-45 7.645341232230403E-4 46-47 6.10748523724153E-4 48-49 0.0021134535245418526 50-51 0.0013928581440327806 52-53 0.0020211821648425205 54-55 9.13925848450531E-4 56-57 2.5923858201241023E-4 58-59 8.040789916656113E-4 60-61 0.0058262772838721345 62-63 0.002574810323038515 64-65 0.0010984685678491958 66-67 0.005545069330502741 68-69 0.00775958196328672 70-71 0.002557234825952928 72-73 6.151423979955498E-4 74-75 0.0032251037152052388 76-77 0.0018937598109720136 78-79 0.0024693573405249925 80-81 9.622584654358957E-4 82-83 1.6257334804168098E-4 84-85 6.283240208097401E-4 86-87 0.004991441172306746 88-89 0.0027725346652513706 90-91 0.0012830112872478608 92-93 1.1424073105631637E-4 94-95 3.998425586971073E-4 96-97 3.2514669608336196E-4 98-99 6.327178950811368E-4 100-101 8.787748542793566E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1379479E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.12752299694709 #Duplication Level Percentage of deduplicated Percentage of total 1 63.92450976102213 29.48679294070117 2 18.71838533671502 17.26865500170079 3 7.636258541723687 10.567250744819793 4 3.672594065363139 6.776306688339585 5 1.9894006819812924 4.588306285411716 6 1.1634216079993047 3.2199454186879852 7 0.7213566764969392 2.329207767887972 8 0.4670166622337281 1.7233857461715014 9 0.32242821611701844 1.3385533458422159 >10 1.2395268443554586 9.450546930995447 >50 0.0717296083574865 2.313915258216576 >100 0.06414332150733787 6.040060441030928 >500 0.006700143187134085 2.1182071348969718 >1k 0.0024327561040237405 1.7256716174514624 >5k 1.915536729487997E-5 0.0542789119175083 >10k+ 7.662146917951989E-5 0.998915765928398 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42472 0.3732332561095284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36410 0.3199619244431138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23736 0.20858599941174813 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.787748542793568E-6 0.0 8.787748542793568E-6 0.0 5 0.0 8.787748542793568E-6 0.0 8.787748542793568E-6 0.0 6 0.0 8.787748542793568E-6 0.0 8.787748542793568E-6 0.0 7 0.0 8.787748542793568E-6 0.0 8.787748542793568E-6 0.0 8 0.0 8.787748542793568E-6 0.0 1.7575497085587136E-5 0.0 9 0.0 8.787748542793568E-6 0.0 1.7575497085587136E-5 0.0 10-11 0.0 1.7575497085587136E-5 0.0 2.63632456283807E-5 0.0 12-13 0.0 1.7575497085587136E-5 0.0 2.63632456283807E-5 0.0 14-15 0.0 1.7575497085587136E-5 0.0 4.3938742713967834E-5 0.0 16-17 0.0 2.196937135698392E-5 0.0 9.666523397072924E-5 0.0 18-19 0.0 2.63632456283807E-5 0.0 1.054529825135228E-4 0.0 20-21 0.0 2.63632456283807E-5 0.0 1.2302847959910995E-4 0.0 22-23 0.0 2.63632456283807E-5 0.0 2.5923858201241023E-4 0.0 24-25 0.0 2.63632456283807E-5 0.0 4.921139183964398E-4 0.0 26-27 0.0 2.63632456283807E-5 0.0 8.392299858367856E-4 0.0 28-29 0.0 3.9544868442571056E-5 0.0 0.0017355803372017297 0.0 30-31 0.0 4.3938742713967834E-5 0.0 0.0044202375170251645 0.0 32-33 0.0 4.3938742713967834E-5 0.0 0.010444239143110155 0.0 34-35 0.0 4.3938742713967834E-5 0.0 0.02140695545024513 0.0 36-37 0.0 4.3938742713967834E-5 0.0 0.038898968924675724 0.0 38-39 0.0 4.3938742713967834E-5 0.0 0.06276210009263167 0.0 40-41 0.0 4.3938742713967834E-5 0.0 0.08822899536964741 0.0 42-43 0.0 4.3938742713967834E-5 0.0 0.11346301531027914 0.0 44-45 0.0 4.3938742713967834E-5 0.0 0.14531860377790584 0.0 46-47 0.0 4.3938742713967834E-5 0.0 0.18425272369675272 0.0 48-49 0.0 5.27264912567614E-5 0.0 0.2251596931634568 0.0 50-51 0.0 5.27264912567614E-5 0.0 0.2673364922945945 0.0 52-53 0.0 5.712036552815819E-5 0.0 0.3094034445689473 0.0 54-55 0.0 6.151423979955498E-5 0.0 0.3562553259248512 0.0 56-57 0.0 1.4939172522749066E-4 0.0 0.4135162954296941 0.0 58-59 0.0 1.6696722231307777E-4 0.0 0.4790772934332055 0.0 60-61 0.0 1.845427193986649E-4 0.0 0.5515103107971815 0.0 62-63 0.0 1.845427193986649E-4 0.0 0.6247957397698085 0.0 64-65 0.0 2.0211821648425204E-4 0.0 0.7020883820779492 0.0 66-67 0.0 2.109059650270456E-4 0.0 0.7944168621428098 0.0 68-69 0.0 2.724202048266006E-4 0.0 0.902321626499772 0.0 70-71 0.0 2.724202048266006E-4 0.0 1.0196424634203376 0.0 72-73 0.0 2.724202048266006E-4 0.0 1.1432772976689003 0.0 74-75 0.0 2.724202048266006E-4 0.0 1.2719650873295691 0.0 76-77 0.0 2.9878345045498126E-4 0.0 1.4152141763256472 0.0 78-79 0.0 2.9878345045498126E-4 0.0 1.5835698629084864 0.0 80-81 0.0 2.9878345045498126E-4 0.0 1.7733061416959424 0.0 82-83 0.0 2.9878345045498126E-4 0.0 1.9727309132518283 0.0 84-85 0.0 3.03177324726378E-4 0.0 2.1744009545603977 0.0 86-87 0.0 3.0757119899777483E-4 0.0 2.3964146337455343 0.0 88-89 0.0 3.0757119899777483E-4 0.0 2.6472345526539485 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22515 0.0 58.631218 1 GTATCAA 42570 0.0 42.953648 1 ATCAACG 55605 0.0 32.519863 3 TCAACGC 56345 0.0 32.058624 4 TGGTATC 8990 0.0 32.018448 2 GTGGTAT 9530 0.0 31.513224 1 TATCAAC 57760 0.0 31.356453 2 CAACGCA 58525 0.0 30.913168 5 AACGCAG 61675 0.0 29.495123 6 CGCAGAG 69785 0.0 25.679316 8 ACGCAGA 70450 0.0 25.59861 7 GTACATG 45090 0.0 24.397167 1 TACATGG 44435 0.0 23.73095 2 GCAGAGT 75325 0.0 23.399714 9 ACATGGG 45635 0.0 22.273855 3 AGAGTAC 70620 0.0 20.980507 10-11 GAGTACT 48625 0.0 20.619936 12-13 AGTACTT 49925 0.0 20.220957 12-13 CAGAGTA 74490 0.0 19.94151 10-11 CATGGGT 11620 0.0 19.456951 4 >>END_MODULE