FastQCFastQC Report
Wed 25 May 2016
SRR2049422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608918
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328280.3416409631136409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270190.2811867059329677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154090.16036144756360707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169600.057.025641
GTATCAA320050.041.988791
ATCAACG386700.034.3581473
TCAACGC389750.034.0177424
CAACGCA405900.032.733955
TATCAAC409100.032.5324062
AACGCAG438400.030.5453436
GTGGTAT71100.029.062831
CGCAGAG480650.027.3674838
ACGCAGA491400.027.0396087
TGGTATC75100.025.8678842
GCAGAGT524800.024.4495939
GTACATG304850.024.214921
TACATGG303500.023.068342
TACCTGG72300.022.5337452
GTACCTG77100.022.4266951
ACATGGG307200.022.0655253
GAGTACT349950.021.42043312-13
CAGAGTA510000.021.27353510-11
AGAGTAC486200.020.78587510-11
Produced by FastQC (version 0.11.5)