FastQCFastQC Report
Wed 25 May 2016
SRR2049422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608918
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298260.31039915212097763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250480.2606745109074716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161120.1676775678593573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166200.054.588541
GTATCAA305750.040.1032681
ATCAACG375950.032.043
TCAACGC381150.031.6027184
CAACGCA396450.030.4310115
TATCAAC400500.030.2665422
GTGGTAT73400.029.0721281
AACGCAG428300.028.4111356
TGGTATC78600.026.8919412
CGCAGAG479100.024.9029628
ACGCAGA489550.024.662317
TATAACG9050.022.5685962
TACCTGG70600.022.2020912
GCAGAGT528200.022.1745329
GTACATG297500.022.1253091
GTACCTG75500.021.6539961
TACATGG297500.021.3146882
AGAGTAC486850.020.52617810-11
ACATGGG298050.020.2867583
GAGTACT347150.019.79743412-13
Produced by FastQC (version 0.11.5)