##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049422_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9608918 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.316482147105425 33.0 31.0 34.0 31.0 34.0 2 32.503895339725034 34.0 31.0 34.0 31.0 34.0 3 32.58327836703362 34.0 31.0 34.0 31.0 34.0 4 36.160970361074995 37.0 35.0 37.0 35.0 37.0 5 36.080542262927004 37.0 35.0 37.0 35.0 37.0 6 36.11763905155607 37.0 36.0 37.0 35.0 37.0 7 36.059931617690985 37.0 35.0 37.0 35.0 37.0 8 36.054339937129235 37.0 35.0 37.0 35.0 37.0 9 37.72739344846111 39.0 38.0 39.0 35.0 39.0 10-11 37.724820057783816 39.0 37.5 39.0 35.0 39.0 12-13 37.71609207196897 39.0 37.0 39.0 35.0 39.0 14-15 39.03459333298504 40.0 38.0 41.0 36.0 41.0 16-17 39.02301918904917 40.0 38.0 41.0 36.0 41.0 18-19 39.04691693695378 40.0 38.5 41.0 36.0 41.0 20-21 39.03402609950464 40.0 38.5 41.0 36.0 41.0 22-23 38.95897940850364 40.0 38.0 41.0 35.0 41.0 24-25 38.87645835878712 40.0 38.0 41.0 35.0 41.0 26-27 38.73009708273086 40.0 38.0 41.0 35.0 41.0 28-29 38.56900303447277 40.0 38.0 41.0 34.5 41.0 30-31 38.40992648704048 40.0 38.0 41.0 34.0 41.0 32-33 38.191077601036866 40.0 38.0 41.0 34.0 41.0 34-35 38.03271741938062 40.0 38.0 41.0 34.0 41.0 36-37 37.86371764229854 40.0 37.0 41.0 33.0 41.0 38-39 38.01692557892574 40.0 38.0 41.0 34.0 41.0 40-41 37.94318777618874 40.0 38.0 41.0 34.0 41.0 42-43 37.777368742245486 40.0 38.0 41.0 33.0 41.0 44-45 37.57658755127268 40.0 37.0 41.0 33.0 41.0 46-47 37.28787429552422 40.0 37.0 41.0 32.5 41.0 48-49 37.05442855272571 40.0 37.0 41.0 32.0 41.0 50-51 36.85421750919302 40.0 37.0 41.0 31.0 41.0 52-53 36.69762474817664 40.0 36.0 41.0 31.0 41.0 54-55 36.610754977823724 39.0 36.0 41.0 31.0 41.0 56-57 36.538092790468184 39.0 36.0 41.0 31.0 41.0 58-59 36.32106752289904 39.0 35.5 41.0 30.5 41.0 60-61 36.09982846143551 39.0 35.0 41.0 30.0 41.0 62-63 35.84630002046016 38.5 35.0 40.0 30.0 41.0 64-65 35.612444033761136 38.0 35.0 40.0 29.0 41.0 66-67 35.29486233517655 38.0 35.0 40.0 29.0 41.0 68-69 34.991649319933835 37.0 34.0 40.0 29.0 41.0 70-71 34.66985471204978 37.0 34.0 39.5 28.0 41.0 72-73 34.30875016312971 36.0 34.0 39.0 28.0 41.0 74-75 33.938807470310394 36.0 34.0 39.0 27.5 40.0 76-77 31.908313089985782 34.0 31.0 36.5 25.0 39.0 78-79 33.200539384351075 35.0 33.5 37.0 27.0 39.0 80-81 33.183312574839334 35.0 34.0 37.0 27.5 39.0 82-83 32.8976438866478 35.0 34.0 36.5 27.0 39.0 84-85 32.62428980037086 35.0 33.5 36.0 27.0 37.5 86-87 32.35372041888587 35.0 33.0 36.0 26.0 37.0 88-89 32.092007497618354 35.0 33.0 35.5 26.0 37.0 90-91 31.83784365731917 35.0 33.0 35.0 25.5 36.0 92-93 31.61462039742664 35.0 33.0 35.0 25.0 36.0 94-95 31.388543434338807 35.0 33.0 35.0 25.0 36.0 96-97 31.183893909803373 35.0 33.0 35.0 24.0 36.0 98-99 30.942826861463487 35.0 32.0 35.0 23.0 35.0 100-101 30.384636542844888 34.5 31.5 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37230870098962754 1101 2 0.38124240958227773 1101 3 0.35894710888522496 1101 4 0.27039678860366223 1101 5 0.23802847823485962 1101 6 0.27520939148172374 1101 7 0.28618236711451317 1101 8 0.2919704950268809 1101 9 0.3478575183659629 1101 10-11 0.3422415764120146 1101 12-13 0.3269919447503682 1101 14-15 0.39968476299495137 1101 16-17 0.37335451385384744 1101 18-19 0.3358273426988845 1101 20-21 0.27918576496306713 1101 22-23 0.24731727983575524 1101 24-25 0.23375447090769086 1101 26-27 0.1895672302137683 1101 28-29 0.21832719611820295 1101 30-31 0.17025526912705402 1101 32-33 0.2406969696006911 1101 34-35 0.24349464580379276 1101 36-37 0.11357895273536656 1101 38-39 0.26031453027322726 1101 40-41 0.3269080288569626 1101 42-43 0.32803863707154335 1101 44-45 0.27459948327929595 1101 46-47 0.28048685675164364 1101 48-49 0.20654575664609354 1101 50-51 0.19531318157946487 1101 52-53 0.21620174948676407 1101 54-55 0.14118280932704863 1101 56-57 0.07788237971085721 1101 58-59 0.07431567432381314 1101 60-61 0.09570952424726187 1101 62-63 0.1112086845219693 1101 64-65 0.0692368777645882 1101 66-67 0.00830727639948492 1101 68-69 -0.01780116972940249 1101 70-71 -0.02708211070229538 1101 72-73 -0.10484798031700393 1101 74-75 -0.0782209550414521 1101 76-77 -0.0844268823637293 1101 78-79 -0.038216397792709245 1101 80-81 -0.04893701614677326 1101 82-83 -0.024444371236768347 1101 84-85 -0.09147391697202778 1101 86-87 -0.15984095137343957 1101 88-89 -0.15017039485172745 1101 90-91 -0.12189181400225735 1101 92-93 -0.20315362559953698 1101 94-95 -0.28848516911345357 1101 96-97 -0.360020953479701 1101 98-99 -0.39676453995327776 1101 100-101 -0.425816483412806 1102 1 0.34305820677688814 1102 2 0.3138580858862241 1102 3 0.28496529299068385 1102 4 0.2621622830402188 1102 5 0.2688676755668453 1102 6 0.2559766366407459 1102 7 0.2397379286528718 1102 8 0.2667607578953408 1102 9 0.3226598077449765 1102 10-11 0.2815549013504395 1102 12-13 0.23845636909079815 1102 14-15 0.3181268473128256 1102 16-17 0.2912549323044047 1102 18-19 0.26483104625152265 1102 20-21 0.2677449959843301 1102 22-23 0.26371749588304283 1102 24-25 0.26985976445853055 1102 26-27 0.2511356344317761 1102 28-29 0.2767256367773996 1102 30-31 0.21914889195429765 1102 32-33 0.2387114507446242 1102 34-35 0.19608004195505657 1102 36-37 0.19569584859088707 1102 38-39 0.29191494983615485 1102 40-41 0.24828287826360906 1102 42-43 0.2985365050528088 1102 44-45 0.27799217910331464 1102 46-47 0.2107453960980763 1102 48-49 0.04113036833354755 1102 50-51 0.05289468469874947 1102 52-53 0.11434346552365326 1102 54-55 0.1423666091766833 1102 56-57 0.05828670153790938 1102 58-59 0.12175138636443705 1102 60-61 0.10550340286085458 1102 62-63 0.08379545206086192 1102 64-65 0.06514176493974588 1102 66-67 0.011407880632226863 1102 68-69 0.04835271511561956 1102 70-71 0.02420350876698052 1102 72-73 -0.06821721208575582 1102 74-75 -0.014475833019382378 1102 76-77 -0.056914847658163126 1102 78-79 0.047773512728007006 1102 80-81 0.017112601323674426 1102 82-83 0.11673197733514229 1102 84-85 0.054172268568656534 1102 86-87 -0.019849912682197157 1102 88-89 -0.03488700528451716 1102 90-91 -0.1242853475442125 1102 92-93 -0.053073311831209224 1102 94-95 -0.09086576260294166 1102 96-97 -0.20283302571034056 1102 98-99 -0.17521702575186637 1102 100-101 -0.14221787631702298 1103 1 0.32657808947894296 1103 2 0.3352591016436861 1103 3 0.3017642642181002 1103 4 0.25287208029586594 1103 5 0.23984063343377926 1103 6 0.273067983543001 1103 7 0.23389525659913346 1103 8 0.27496965685168107 1103 9 0.3464359693960759 1103 10-11 0.3091608509120576 1103 12-13 0.2893115733592353 1103 14-15 0.34912377352295465 1103 16-17 0.38015052110357317 1103 18-19 0.34591562219437577 1103 20-21 0.3232291927296771 1103 22-23 0.2808679001321792 1103 24-25 0.31755557099669574 1103 26-27 0.2650768277663502 1103 28-29 0.2725618254266138 1103 30-31 0.28316057318988186 1103 32-33 0.2975000201161677 1103 34-35 0.2772688379598165 1103 36-37 0.28626692766410144 1103 38-39 0.3357376896791777 1103 40-41 0.36006725620486435 1103 42-43 0.4260748044803506 1103 44-45 0.3978741595867419 1103 46-47 0.4105711165357775 1103 48-49 0.36115573088846986 1103 50-51 0.40861011248040313 1103 52-53 0.36766215602087726 1103 54-55 0.2958722103443421 1103 56-57 0.3188698328106767 1103 58-59 0.3174156181052652 1103 60-61 0.31748858300755245 1103 62-63 0.33389108862618144 1103 64-65 0.28822436871200097 1103 66-67 0.25932616851131485 1103 68-69 0.20667045776358606 1103 70-71 0.2375929241791468 1103 72-73 0.2469486371719043 1103 74-75 0.1983312053115469 1103 76-77 0.1803665059605919 1103 78-79 0.2657973865959704 1103 80-81 0.26772629810258763 1103 82-83 0.29527128175954687 1103 84-85 0.2589127556595301 1103 86-87 0.27485241619849177 1103 88-89 0.27243528815474605 1103 90-91 0.2709741870430129 1103 92-93 0.22780786363069794 1103 94-95 0.19505707899916302 1103 96-97 0.21501012194033464 1103 98-99 0.18161767346713376 1103 100-101 0.12775060908882097 1104 1 0.3335764658646099 1104 2 0.29549526233083157 1104 3 0.2925106520647418 1104 4 0.23391032411144863 1104 5 0.2309250617840064 1104 6 0.24057991234874976 1104 7 0.22523105255181974 1104 8 0.21414430256531602 1104 9 0.28764251081827297 1104 10-11 0.2505068926013365 1104 12-13 0.2287176284337349 1104 14-15 0.28509757766559574 1104 16-17 0.3070153534292501 1104 18-19 0.29895275660492615 1104 20-21 0.28365743821193945 1104 22-23 0.2556566797089346 1104 24-25 0.26452670521734234 1104 26-27 0.26256356153075444 1104 28-29 0.26682060577801536 1104 30-31 0.2521436545910163 1104 32-33 0.26893887081609336 1104 34-35 0.25601400882421643 1104 36-37 0.2899194578854818 1104 38-39 0.3255335390772558 1104 40-41 0.35476822923212836 1104 42-43 0.3780314973322305 1104 44-45 0.32859881971281624 1104 46-47 0.4069033128210151 1104 48-49 0.3647101394126011 1104 50-51 0.34371775929272275 1104 52-53 0.3544347137816928 1104 54-55 0.3308898229739867 1104 56-57 0.3327431885045229 1104 58-59 0.37109755599881566 1104 60-61 0.36002723511462165 1104 62-63 0.3893781217287966 1104 64-65 0.4158625042299704 1104 66-67 0.3977274574059635 1104 68-69 0.3895603518530706 1104 70-71 0.3627524332790557 1104 72-73 0.3142468008153543 1104 74-75 0.35266295522021807 1104 76-77 0.27400879469503536 1104 78-79 0.41130425469992815 1104 80-81 0.4015048181009746 1104 82-83 0.40774285353365514 1104 84-85 0.36753445092886494 1104 86-87 0.29817857184999497 1104 88-89 0.2997360985291735 1104 90-91 0.2917397787147742 1104 92-93 0.23246360789289966 1104 94-95 0.21244817469114707 1104 96-97 0.20049944318325075 1104 98-99 0.24509177225311518 1104 100-101 0.2496929255310789 1105 1 0.31145343227395017 1105 2 0.26990182978414623 1105 3 0.25198018030825153 1105 4 0.19796183289587077 1105 5 0.21440611145857247 1105 6 0.21304923001500242 1105 7 0.20343787513888856 1105 8 0.2173845821127003 1105 9 0.27460368626719145 1105 10-11 0.23719371079649676 1105 12-13 0.20582515827262426 1105 14-15 0.26692212755904166 1105 16-17 0.266359844357531 1105 18-19 0.30881336047983865 1105 20-21 0.26219826243065825 1105 22-23 0.14613397901149483 1105 24-25 0.19177689522222607 1105 26-27 0.19369413244177736 1105 28-29 0.18994919666682364 1105 30-31 0.2338883344221685 1105 32-33 0.287525119178035 1105 34-35 0.19505254204283062 1105 36-37 0.16748196678184968 1105 38-39 0.23295463134545003 1105 40-41 0.23916752596250745 1105 42-43 0.26829660643728914 1105 44-45 0.2063138695113267 1105 46-47 0.26978457127500377 1105 48-49 0.2192655856061947 1105 50-51 0.22027094113415302 1105 52-53 0.22374917578925846 1105 54-55 0.22664981238444426 1105 56-57 0.1924953029491121 1105 58-59 0.1676431281047499 1105 60-61 0.22712099762075155 1105 62-63 0.24207544073736642 1105 64-65 0.21051258597309896 1105 66-67 0.23295448129647411 1105 68-69 0.22303696151536911 1105 70-71 0.1323434182081229 1105 72-73 0.1677922757612933 1105 74-75 0.17641059269259785 1105 76-77 0.23719027941212545 1105 78-79 0.2613725002799967 1105 80-81 0.22054741455288251 1105 82-83 0.30234195356987215 1105 84-85 0.24440921279269645 1105 86-87 0.21339352563428093 1105 88-89 0.24956210600945639 1105 90-91 0.284518053766714 1105 92-93 0.1996576264562968 1105 94-95 0.23975700885407747 1105 96-97 0.27453365544231545 1105 98-99 0.23680397719821755 1105 100-101 0.3113697461994427 1106 1 0.23889325684696416 1106 2 0.21809485327942468 1106 3 0.20145757006720544 1106 4 0.18177814139778548 1106 5 0.2062202869664702 1106 6 0.24776605191326695 1106 7 0.22927168301492173 1106 8 0.20679160206070435 1106 9 0.3262642296267444 1106 10-11 0.22582373198498118 1106 12-13 0.20823839213349515 1106 14-15 0.2813442542363731 1106 16-17 0.28059358821604263 1106 18-19 0.2841562552921957 1106 20-21 0.23762678264973403 1106 22-23 0.24943393757617827 1106 24-25 0.31514985670400364 1106 26-27 0.2541150406893067 1106 28-29 0.31561099703287 1106 30-31 0.3244357158416378 1106 32-33 0.3283631314183211 1106 34-35 0.34770557946725944 1106 36-37 0.3439152415280802 1106 38-39 0.39576568679073887 1106 40-41 0.3891966266321347 1106 42-43 0.4648622625233543 1106 44-45 0.4922147681799558 1106 46-47 0.5325287406808599 1106 48-49 0.5312075528173992 1106 50-51 0.5503841597522907 1106 52-53 0.47335532265782376 1106 54-55 0.4410455783260403 1106 56-57 0.39181817329593116 1106 58-59 0.4794184638134169 1106 60-61 0.5587514224796521 1106 62-63 0.5080808189209449 1106 64-65 0.45119101437472153 1106 66-67 0.49686007603374804 1106 68-69 0.5286337908282661 1106 70-71 0.5227366363079469 1106 72-73 0.49750099803912207 1106 74-75 0.49102465322218336 1106 76-77 0.4615247104005604 1106 78-79 0.4882734233805408 1106 80-81 0.552470680973471 1106 82-83 0.5429607364793654 1106 84-85 0.5109305788861391 1106 86-87 0.5361314604541221 1106 88-89 0.5678761307220768 1106 90-91 0.5422136575972054 1106 92-93 0.47568464418268164 1106 94-95 0.5687999667640433 1106 96-97 0.5544945159319035 1106 98-99 0.5506554854427996 1106 100-101 0.6644829985474523 1107 1 0.20612237128633382 1107 2 0.18696066638899111 1107 3 0.1747469983865244 1107 4 0.1600927849081657 1107 5 0.12913572403952145 1107 6 0.15662643046253066 1107 7 0.18317570361561053 1107 8 0.16774019579518296 1107 9 0.22824394839312134 1107 10-11 0.1724539150264306 1107 12-13 0.17246048060190589 1107 14-15 0.15478858700287645 1107 16-17 0.21563364618468484 1107 18-19 0.20275340828383293 1107 20-21 0.18666024902266543 1107 22-23 0.16004691312455321 1107 24-25 0.20397438468614126 1107 26-27 0.23528492231717735 1107 28-29 0.22662757146783719 1107 30-31 0.2411700223313602 1107 32-33 0.2538488330706983 1107 34-35 0.25358001774993966 1107 36-37 0.28583401140853937 1107 38-39 0.297373362181915 1107 40-41 0.3662769042427172 1107 42-43 0.30650716173076376 1107 44-45 0.2895546580473294 1107 46-47 0.3140140800292244 1107 48-49 0.28143073014573616 1107 50-51 0.3428530084241359 1107 52-53 0.316287472464694 1107 54-55 0.367401296593755 1107 56-57 0.40670234314249853 1107 58-59 0.4407390522263199 1107 60-61 0.4401463319133825 1107 62-63 0.47313498921552366 1107 64-65 0.5234812335297008 1107 66-67 0.5438181344737743 1107 68-69 0.567535113627514 1107 70-71 0.5547957210812413 1107 72-73 0.5334269477723552 1107 74-75 0.5199491264691503 1107 76-77 0.5192475809392079 1107 78-79 0.6180702506881417 1107 80-81 0.5563376433269624 1107 82-83 0.6041061625859072 1107 84-85 0.6092240003411646 1107 86-87 0.5866751599677329 1107 88-89 0.5522453905895119 1107 90-91 0.5987393665735716 1107 92-93 0.6015730681796647 1107 94-95 0.6538393429778147 1107 96-97 0.6875198908483888 1107 98-99 0.7006562158944512 1107 100-101 0.7776316152668059 1108 1 0.12370187077662109 1108 2 0.13631746566193215 1108 3 0.11764884562672506 1108 4 0.12581779454944808 1108 5 0.09189644978160061 1108 6 0.12491482594353442 1108 7 0.1464784713368914 1108 8 0.13039490477787297 1108 9 0.1924638713226372 1108 10-11 0.12258666953391639 1108 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-1.2506525604816652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 42.0 5 95.0 6 242.0 7 661.0 8 1870.0 9 4181.0 10 7308.0 11 11552.0 12 17644.0 13 25367.0 14 31329.0 15 39657.0 16 50612.0 17 69065.0 18 58490.0 19 33041.0 20 25365.0 21 24593.0 22 24732.0 23 26437.0 24 30344.0 25 37151.0 26 46606.0 27 59606.0 28 77618.0 29 100367.0 30 130757.0 31 173291.0 32 235557.0 33 330443.0 34 492130.0 35 777121.0 36 1288643.0 37 2153699.0 38 2554617.0 39 668291.0 40 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.09284493276357 22.312630395068293 15.93088048830617 22.663644183861965 2 14.656582326024987 22.218131019484126 39.886028203835835 23.239258450655047 3 16.93906002540562 27.191245031428164 31.879701501670638 23.98999344149558 4 12.294132112825068 18.614506374459577 39.10942706927843 29.98193444343693 5 12.416348915176743 38.04854411571559 35.93265118494126 13.602455784166413 6 28.860430310276865 39.30105931672006 18.30146113835309 13.537049234649992 7 25.332165390525756 33.61286879542525 23.703470047303973 17.351495766745018 8 24.60331121568526 36.00648897201537 21.258782726629573 18.131417085669792 9 24.689564035697718 16.953813472626333 22.634262422798184 35.72236006887777 10-11 23.02940071185962 27.67902459767318 29.710252466537074 19.581322223930126 12-13 23.97002451264544 25.331187132619924 29.888094580472014 20.810693774262617 14-15 21.226049473038973 27.252928804323506 27.71256661813512 23.8084551045024 16-17 20.14687813966151 30.434347550889708 28.49073641798171 20.92803789146707 18-19 20.16824795019749 28.80005111920302 31.224076478573775 19.807624452025717 20-21 21.155191465881614 27.963440222810892 31.033760903732034 19.847607407575463 22-23 20.78614886712531 27.884955413294193 31.184515259678562 20.144380459901935 24-25 20.675857560195233 28.01915919465568 30.99927369108028 20.3057095540688 26-27 20.397109705937925 28.12134997025726 31.021385684444468 20.460154639360344 28-29 20.41005259410276 28.08288414700496 31.19807010808937 20.30899315080291 30-31 20.671489913190616 28.337557667771918 30.843752112318988 20.147200306718474 32-33 20.39615872576437 28.198302087680716 31.13245152353129 20.273087663023624 34-35 20.627340428803738 28.075164533520685 30.95327887573761 20.344216161937965 36-37 20.88162118869888 28.0984074432997 30.63279880726198 20.38717256073944 38-39 20.617551487991708 28.30329059503277 30.758770267172704 20.32038764980282 40-41 20.51792871184767 28.151107916333007 30.83720017061558 20.493763201203738 42-43 20.529210288622217 28.266902312039534 30.61380575637575 20.590081642962502 44-45 20.672422127737516 28.46944664004883 30.36069371500945 20.4974375172042 46-47 20.901601661340596 28.581154707477722 29.969091567668254 20.548152063513424 48-49 20.874211537074576 28.510004499035098 29.85789376678097 20.757890197109358 50-51 20.881847720584602 28.591782701475278 29.610110658568317 20.916258919371796 52-53 20.805441768511283 28.771980892969605 29.51857920224396 20.90399813627515 54-55 20.872521416863833 29.08448432623225 29.45752617524183 20.585468081662082 56-57 20.853886126835118 28.774679287961646 29.40755139054787 20.963883194655367 58-59 20.784260506566447 28.561285390298607 29.635821068248763 21.01863303488618 60-61 20.946776058273038 28.776290466801573 29.45956042733436 20.81737304759103 62-63 20.756741685905805 28.75848511164684 29.52949204148317 20.955281160964184 64-65 20.778725090206176 28.767670872420247 29.5158844149558 20.937719622417777 66-67 20.713478943581993 28.73910752686773 29.49693822991051 21.050475299639768 68-69 20.931463219272235 28.92491442711755 29.223253842765708 20.920368510844504 70-71 20.943417580922254 28.66743177329462 29.194382268328674 21.194768377454455 72-73 20.73690443253404 28.84027016075717 29.38691083547116 21.035914571237633 74-75 20.800119767876367 29.005660718150377 29.138869697431563 21.055349816541693 76-77 20.808938835166877 29.05852535674872 29.08779563238574 21.044740175698667 78-79 20.855324516201783 28.967229163255038 29.248922367644546 20.92852395289863 80-81 20.81496286150536 28.924971670589883 29.16422555273506 21.095839915169698 82-83 20.61668134576472 28.94489182477788 29.37671019817979 21.061716631277605 84-85 20.62073274402612 29.017749099827572 29.126809977902152 21.23470817824416 86-87 20.710286966545972 29.05953813904203 29.040612098438935 21.189562795973067 88-89 20.558752092643076 28.989499266391093 29.180993149722617 21.27075549124321 90-91 20.478486462173183 28.917325095327996 29.17852320454009 21.425665237958732 92-93 20.61071780700113 28.985911611582004 29.029506584828702 21.373863996588167 94-95 20.48678153485077 28.830338929586823 29.237015303257856 21.44586423230455 96-97 20.489922506113352 28.95857761674167 29.095445705626467 21.456054171518513 98-99 20.3637776914917 28.954086451227372 29.036250178611144 21.645885678669785 100-101 20.311513774132923 29.046993537357018 28.921578779377533 21.719913909132526 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5007.0 1 3380.0 2 2457.0 3 3138.5 4 2568.0 5 2187.0 6 3450.5 7 6695.0 8 10338.5 9 12109.0 10 11051.0 11 9721.0 12 9902.5 13 10493.5 14 11187.0 15 11866.5 16 12697.5 17 13665.0 18 14990.0 19 16657.0 20 19201.5 21 23222.0 22 29876.0 23 41109.5 24 53893.5 25 67648.5 26 87343.5 27 113130.0 28 147025.0 29 178563.0 30 199898.5 31 224961.5 32 253428.5 33 278381.5 34 302058.5 35 322997.0 36 335685.0 37 344717.5 38 355482.0 39 360907.0 40 363538.5 41 360236.5 42 349981.0 43 340509.5 44 330895.5 45 320617.5 46 310302.5 47 299466.0 48 287116.5 49 277337.0 50 268362.5 51 252864.0 52 230946.5 53 212683.5 54 199395.5 55 187406.0 56 170689.5 57 150252.5 58 133103.0 59 118159.5 60 102747.5 61 86471.0 62 72284.5 63 59404.5 64 48119.5 65 39532.5 66 31781.0 67 24615.0 68 18592.0 69 13932.5 70 10402.0 71 7489.5 72 5652.5 73 4110.0 74 2971.5 75 2107.0 76 1359.0 77 942.0 78 666.5 79 419.0 80 270.5 81 185.0 82 118.5 83 79.5 84 63.0 85 44.5 86 30.5 87 21.5 88 17.5 89 13.0 90 8.0 91 6.0 92 3.0 93 2.5 94 2.5 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04633195954008557 2 0.0023415747746000125 3 2.081399799644455E-4 4 2.6017497495555694E-4 5 0.0026641917435449025 6 0.0018420388226853429 7 0.0 8 0.0 9 1.248839879786673E-4 10-11 1.0406998998222275E-5 12-13 0.0 14-15 4.995359519146692E-4 16-17 0.0 18-19 4.683149549200024E-5 20-21 5.411639479075584E-4 22-23 0.0 24-25 6.140129408951144E-4 26-27 0.007196439807270704 28-29 0.007404579787235149 30-31 0.0011083453933106724 32-33 0.006743735350848035 34-35 0.008008185729132042 36-37 0.004553062061722246 38-39 0.002554918254063569 40-41 0.0018992773171755653 42-43 0.0013425028707706736 44-45 7.597109268702262E-4 46-47 5.359604484084472E-4 48-49 0.001987736808660455 50-51 0.0013268923722733403 52-53 0.002117824296138233 54-55 7.961354233640041E-4 56-57 2.653784744546681E-4 58-59 7.284899298755593E-4 60-61 0.005864343935498253 62-63 0.002596546250056458 64-65 0.0010094789028275608 66-67 0.005260737993601361 68-69 0.00763873726469515 70-71 0.002450848264081346 72-73 4.8392545341733586E-4 74-75 0.003449920167910685 76-77 0.0020189578056551217 78-79 0.002471662262077791 80-81 9.106124123444493E-4 82-83 1.769189829697787E-4 84-85 6.712514353853368E-4 86-87 0.004823644035676025 88-89 0.0026641917435449025 90-91 0.001243636380287562 92-93 1.248839879786673E-4 94-95 5.099429509128915E-4 96-97 3.2261696894489054E-4 98-99 5.723849449022251E-4 100-101 1.0406998998222275E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9608918.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.125862020087894 #Duplication Level Percentage of deduplicated Percentage of total 1 68.63827823497932 33.71914585867996 2 17.4796543874806 17.17406179196392 3 6.079475950575535 8.959784901072492 4 2.6470794444500805 5.201602381770623 5 1.423401774205444 3.4962919579382468 6 0.8579256714475689 2.528780289541472 7 0.5671584304701451 1.9503502759164175 8 0.38965521456185553 1.5313718644778767 9 0.2772795426696432 1.225943689876377 >10 1.482423489452725 13.110996666967296 >50 0.09980876230685337 3.3788982259023106 >100 0.053222581875666794 4.669170421176593 >500 0.0031273636019698826 1.0595103409068733 >1k 0.0014241293930382394 1.1823814802853523 >5k 2.1255632416193008E-5 0.07047314339021107 >10k+ 6.376689724857902E-5 0.7412367101344679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29826 0.31039915212097763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25048 0.2606745109074716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16112 0.1676775678593573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0406998998222277E-5 0.0 3 0.0 0.0 0.0 1.0406998998222277E-5 0.0 4 0.0 0.0 0.0 2.0813997996444554E-5 0.0 5 0.0 1.0406998998222277E-5 0.0 2.0813997996444554E-5 0.0 6 0.0 1.0406998998222277E-5 0.0 2.0813997996444554E-5 0.0 7 0.0 1.0406998998222277E-5 0.0 2.0813997996444554E-5 0.0 8 0.0 1.0406998998222277E-5 0.0 2.0813997996444554E-5 0.0 9 0.0 1.0406998998222277E-5 0.0 2.0813997996444554E-5 0.0 10-11 2.0813997996444554E-5 1.5610498497333417E-5 0.0 2.0813997996444554E-5 0.0 12-13 2.0813997996444554E-5 2.0813997996444554E-5 0.0 4.162799599288911E-5 0.0 14-15 2.0813997996444554E-5 2.601749749555569E-5 0.0 5.2034994991111384E-5 0.0 16-17 2.0813997996444554E-5 3.1220996994666826E-5 0.0 5.2034994991111384E-5 0.0 18-19 2.0813997996444554E-5 3.1220996994666826E-5 0.0 5.7238494490222515E-5 0.0 20-21 2.0813997996444554E-5 3.1220996994666826E-5 0.0 8.325599198577822E-5 0.0 22-23 2.0813997996444554E-5 4.162799599288911E-5 0.0 1.6651198397155643E-4 0.0 24-25 2.0813997996444554E-5 5.2034994991111384E-5 0.0 3.902624624333354E-4 0.0 26-27 2.0813997996444554E-5 6.244199398933365E-5 0.0 5.723849449022253E-4 0.0 28-29 2.0813997996444554E-5 6.244199398933365E-5 0.0 0.0014049448647600073 0.0 30-31 2.0813997996444554E-5 6.244199398933365E-5 0.0 0.0038557931288413535 0.0 32-33 3.1220996994666826E-5 6.244199398933365E-5 0.0 0.009199787114428492 0.0 34-35 3.1220996994666826E-5 6.244199398933365E-5 0.0 0.019102046661236986 0.0 36-37 3.1220996994666826E-5 6.244199398933365E-5 0.0 0.03543062808944774 0.0 38-39 3.1220996994666826E-5 6.244199398933365E-5 0.0 0.056140556095910066 0.0 40-41 3.1220996994666826E-5 6.244199398933365E-5 0.0 0.0770898450793315 0.0 42-43 4.162799599288911E-5 6.244199398933365E-5 0.0 0.0994596894260103 0.0 44-45 4.162799599288911E-5 6.244199398933365E-5 0.0 0.12891149659097934 0.0 46-47 4.162799599288911E-5 8.845949148488935E-5 0.0 0.16410796720296708 0.0 48-49 5.2034994991111384E-5 9.366299098400049E-5 0.0 0.20062092318822994 0.0 50-51 5.2034994991111384E-5 9.366299098400049E-5 0.0 0.23869492902322614 0.0 52-53 5.2034994991111384E-5 9.366299098400049E-5 0.0 0.2773100988061299 0.0 54-55 5.2034994991111384E-5 1.0406998998222277E-4 0.0 0.3222683344784501 0.0 56-57 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.3782840065863815 0.0 58-59 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.43935227670794985 0.0 60-61 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.5047914864087715 0.0 62-63 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.5710424420314546 0.0 64-65 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.6441203889969713 0.0 66-67 5.2034994991111384E-5 1.1447698898044504E-4 0.0 0.7321844145199283 0.0 68-69 5.2034994991111384E-5 1.248839879786673E-4 0.0 0.8337775387405741 0.0 70-71 6.76454934884448E-5 1.248839879786673E-4 0.0 0.9461471104238791 0.0 72-73 8.325599198577822E-5 1.248839879786673E-4 0.0 1.064079223071734 0.0 74-75 8.325599198577822E-5 1.3529098697688958E-4 0.0 1.1867257062657837 0.0 76-77 1.0406998998222277E-4 1.4569798597511188E-4 0.0 1.3247797514767012 0.0 78-79 1.0406998998222277E-4 1.4569798597511188E-4 0.0 1.4859633519611677 0.0 80-81 1.0406998998222277E-4 1.6651198397155643E-4 0.0 1.6652603342020402 0.0 82-83 1.0406998998222277E-4 1.6651198397155643E-4 0.0 1.854782192958666 0.0 84-85 1.0406998998222277E-4 1.6651198397155643E-4 0.0 2.0505066231182325 0.0 86-87 1.0406998998222277E-4 1.6651198397155643E-4 0.0 2.2642819930402154 0.0 88-89 1.0406998998222277E-4 1.6651198397155643E-4 0.0 2.5084926315324996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16620 0.0 54.58854 1 GTATCAA 30575 0.0 40.103268 1 ATCAACG 37595 0.0 32.04 3 TCAACGC 38115 0.0 31.602718 4 CAACGCA 39645 0.0 30.431011 5 TATCAAC 40050 0.0 30.266542 2 GTGGTAT 7340 0.0 29.072128 1 AACGCAG 42830 0.0 28.411135 6 TGGTATC 7860 0.0 26.891941 2 CGCAGAG 47910 0.0 24.902962 8 ACGCAGA 48955 0.0 24.66231 7 TATAACG 905 0.0 22.568596 2 TACCTGG 7060 0.0 22.202091 2 GCAGAGT 52820 0.0 22.174532 9 GTACATG 29750 0.0 22.125309 1 GTACCTG 7550 0.0 21.653996 1 TACATGG 29750 0.0 21.314688 2 AGAGTAC 48685 0.0 20.526178 10-11 ACATGGG 29805 0.0 20.286758 3 GAGTACT 34715 0.0 19.797434 12-13 >>END_MODULE