FastQCFastQC Report
Wed 25 May 2016
SRR2049421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12050490
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357130.2963613927732399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283340.23512736826469294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163660.13581190474412244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203650.054.417921
GTATCAA384650.042.1238021
ATCAACG461200.034.7495653
TCAACGC465700.034.3741264
CAACGCA487100.032.853525
TATCAAC489450.032.849422
AACGCAG514200.031.2236466
ACGCAGA590600.026.9685237
CGCAGAG587650.026.9502038
GTACATG405250.026.4791971
GTGGTAT94600.026.2114451
TACATGG396350.025.5377122
GCAGAGT634050.024.2212989
ACATGGG409950.024.0657393
TGGTATC96050.023.8851032
CATGGGT131400.021.979424
GAGTACT404150.021.0735912-13
CAGAGTA616150.020.49137710-11
AGAGTAC583050.020.36745610-11
GTACTTT439400.019.18702114-15
Produced by FastQC (version 0.11.5)