##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049421_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12050490 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.555069627874055 31.0 31.0 34.0 30.0 34.0 2 31.605045935891404 33.0 31.0 34.0 30.0 34.0 3 31.63810550442347 33.0 31.0 34.0 30.0 34.0 4 35.268114574594065 37.0 35.0 37.0 33.0 37.0 5 35.28995849961288 37.0 35.0 37.0 33.0 37.0 6 35.38005732546975 37.0 35.0 37.0 33.0 37.0 7 35.32622117440867 37.0 35.0 37.0 33.0 37.0 8 35.2994655818975 37.0 35.0 37.0 33.0 37.0 9 36.897377202088876 39.0 37.0 39.0 33.0 39.0 10-11 36.83066091088412 39.0 37.0 39.0 33.0 39.0 12-13 36.79818845540721 39.0 37.0 39.0 33.0 39.0 14-15 37.99648964481942 40.0 38.0 41.0 33.0 41.0 16-17 37.98610110460238 40.0 38.0 41.0 33.0 41.0 18-19 38.00383872357058 40.0 38.0 41.0 33.0 41.0 20-21 37.966352073650114 40.0 38.0 41.0 33.0 41.0 22-23 37.861829311505176 40.0 38.0 41.0 33.0 41.0 24-25 37.7606314763964 40.0 38.0 41.0 33.0 41.0 26-27 37.626709287340184 40.0 37.5 41.0 32.5 41.0 28-29 37.47541137331345 40.0 37.0 41.0 32.0 41.0 30-31 37.302399985394786 40.0 37.0 41.0 32.0 41.0 32-33 37.075139724608704 40.0 36.5 41.0 31.5 41.0 34-35 36.890638098533756 40.0 36.0 41.0 31.0 41.0 36-37 36.71381769538002 39.0 36.0 41.0 30.5 41.0 38-39 36.51856970131505 39.0 36.0 41.0 30.0 41.0 40-41 36.31583039361885 39.0 35.5 40.5 30.0 41.0 42-43 36.097063023993215 39.0 35.0 40.0 30.0 41.0 44-45 35.836963185729374 39.0 35.0 40.0 29.0 41.0 46-47 35.514229462868315 39.0 35.0 40.0 27.5 41.0 48-49 35.249822289384085 38.5 35.0 40.0 27.0 41.0 50-51 33.92063920222331 37.0 33.5 39.0 25.0 40.0 52-53 34.23840167495264 37.5 33.5 39.5 25.5 40.0 54-55 35.30478569751106 39.0 35.0 40.0 27.5 41.0 56-57 35.44039831575313 39.0 35.0 40.5 28.0 41.0 58-59 35.297737353418825 39.0 35.0 41.0 27.0 41.0 60-61 35.05891403586078 38.5 35.0 40.0 26.5 41.0 62-63 34.80258192820375 38.0 34.0 40.0 26.0 41.0 64-65 34.53832931274994 38.0 34.0 40.0 26.0 41.0 66-67 34.23189575693603 37.5 34.0 40.0 25.5 41.0 68-69 33.900674661362316 37.0 34.0 40.0 25.0 41.0 70-71 33.56811424265735 36.0 33.5 39.0 24.0 41.0 72-73 33.311023742603 36.0 33.0 39.0 23.5 41.0 74-75 33.111517083537684 36.0 33.0 39.0 24.0 40.5 76-77 32.76143497069414 35.0 33.0 38.5 22.5 40.0 78-79 32.37470725256816 35.0 33.0 37.0 22.0 39.0 80-81 32.015410991586236 35.0 33.0 37.0 20.5 39.0 82-83 31.651818764216227 35.0 32.0 36.5 20.0 39.0 84-85 31.31636920988275 35.0 32.0 36.0 18.5 37.5 86-87 31.005094025222213 35.0 32.0 36.0 17.5 37.0 88-89 30.697402636739252 35.0 32.0 35.0 13.0 37.0 90-91 30.416805042782492 35.0 31.5 35.0 7.0 36.0 92-93 30.15858836445655 34.5 31.0 35.0 3.5 36.0 94-95 29.89373083584153 34.0 31.0 35.0 2.0 36.0 96-97 29.63117230087739 34.0 31.0 35.0 2.0 35.5 98-99 29.308834329558383 34.0 31.0 35.0 2.0 35.0 100-101 28.761754999174308 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4159634322040269 1101 2 0.43233688666027703 1101 3 0.4202039076350239 1101 4 0.3754461573886374 1101 5 0.3601967228453802 1101 6 0.38218181645312654 1101 7 0.40521913568996126 1101 8 0.3605876700287993 1101 9 0.4463366289825075 1101 10-11 0.4465867721483221 1101 12-13 0.4568316662348195 1101 14-15 0.5949612984931392 1101 16-17 0.5654603345119611 1101 18-19 0.5376064923966766 1101 20-21 0.4507485920346781 1101 22-23 0.48689714177963594 1101 24-25 0.48349328499137556 1101 26-27 0.49412511607796716 1101 28-29 0.4587344501321837 1101 30-31 0.4157825425479942 1101 32-33 0.401648073574421 1101 34-35 0.4289249005295446 1101 36-37 0.4106575644884316 1101 38-39 0.3666944120627349 1101 40-41 0.29776262064027037 1101 42-43 0.25575210988869657 1101 44-45 0.17736826712121712 1101 46-47 0.15233705463236902 1101 48-49 0.06446400087742887 1101 50-51 0.0712870565062218 1101 52-53 0.12026567173346336 1101 54-55 0.33712072766225987 1101 56-57 0.3866048758234797 1101 58-59 0.35456280725987455 1101 60-61 0.3617018102097873 1101 62-63 0.2933539231637283 1101 64-65 0.2735193867291912 1101 66-67 0.304093422502433 1101 68-69 0.2514409166791651 1101 70-71 0.24134773659698538 1101 72-73 0.2024317712751369 1101 74-75 0.2188207471135044 1101 76-77 0.10449395569689557 1101 78-79 0.08372213065453593 1101 80-81 0.008498689347341326 1101 82-83 -0.03450097759616355 1101 84-85 -0.07584334256847924 1101 86-87 -0.1284756104431537 1101 88-89 -0.10033585268800493 1101 90-91 -0.14665226887370864 1101 92-93 -0.21296972895961375 1101 94-95 -0.3312167114102671 1101 96-97 -0.3477347299258646 1101 98-99 -0.46366154400495674 1101 100-101 -0.5268327948165776 1102 1 0.34463416469459673 1102 2 0.36029419927863415 1102 3 0.36918480449073243 1102 4 0.3496023789499034 1102 5 0.3635301298411875 1102 6 0.36957512626916156 1102 7 0.38175516281332733 1102 8 0.40131664475574524 1102 9 0.4987089734470729 1102 10-11 0.4870013977915377 1102 12-13 0.4585410594794439 1102 14-15 0.5550551952414509 1102 16-17 0.5092064233484663 1102 18-19 0.49587872837045666 1102 20-21 0.432248240856822 1102 22-23 0.40410169728410494 1102 24-25 0.4457681392597692 1102 26-27 0.4992547000798311 1102 28-29 0.5462890401262115 1102 30-31 0.42975755884674527 1102 32-33 0.4216756733530147 1102 34-35 0.3958531441354509 1102 36-37 0.3639545085573985 1102 38-39 0.3703689379245887 1102 40-41 0.40126334986184986 1102 42-43 0.3478669456030161 1102 44-45 0.35094139966098936 1102 46-47 0.3403482423614861 1102 48-49 0.24208175410630162 1102 50-51 0.2356226370871468 1102 52-53 0.37659554270858564 1102 54-55 0.5426601509707822 1102 56-57 0.5249505380138046 1102 58-59 0.5071354668569583 1102 60-61 0.5701314276701908 1102 62-63 0.5797735246457805 1102 64-65 0.4963401013966404 1102 66-67 0.5209850147139505 1102 68-69 0.5442468370138656 1102 70-71 0.4423566772756047 1102 72-73 0.4457025412670603 1102 74-75 0.34919770955030316 1102 76-77 0.3082691601823342 1102 78-79 0.2909875508288877 1102 80-81 0.2730531768298121 1102 82-83 0.19376765014798636 1102 84-85 0.21037200443980808 1102 86-87 0.18785040544680598 1102 88-89 0.0326277676804132 1102 90-91 0.013608377306855601 1102 92-93 -0.039492999416971486 1102 94-95 -0.1265581632356394 1102 96-97 -0.20761447211113548 1102 98-99 -0.20272962816435935 1102 100-101 -0.2983503229265132 1103 1 0.34659682950564985 1103 2 0.313141679605657 1103 3 0.3527935739278085 1103 4 0.32514831807660016 1103 5 0.3506603762184852 1103 6 0.3508510103831597 1103 7 0.37302580787307704 1103 8 0.3469928314596302 1103 9 0.47044346780810287 1103 10-11 0.43096899588687165 1103 12-13 0.44980343858966876 1103 14-15 0.4677168813016266 1103 16-17 0.4859646923836962 1103 18-19 0.47443822008277436 1103 20-21 0.4601615174053322 1103 22-23 0.4310438054575556 1103 24-25 0.45187858807746295 1103 26-27 0.47028806228715325 1103 28-29 0.4544838154805433 1103 30-31 0.44993927459024263 1103 32-33 0.36205536518368575 1103 34-35 0.4340940264370019 1103 36-37 0.40840949735019194 1103 38-39 0.37964965555608643 1103 40-41 0.3424551150983888 1103 42-43 0.3570654247553122 1103 44-45 0.34278185493433 1103 46-47 0.28750745829562163 1103 48-49 0.2668120015445581 1103 50-51 0.2403785648237502 1103 52-53 0.33516510554714785 1103 54-55 0.44142371297838423 1103 56-57 0.4640611399480363 1103 58-59 0.48453194718898374 1103 60-61 0.4930065013225686 1103 62-63 0.4709061872906375 1103 64-65 0.43924982285007275 1103 66-67 0.41922979365989477 1103 68-69 0.4085128375713012 1103 70-71 0.35774060786132367 1103 72-73 0.41037976720007663 1103 74-75 0.3962106974691082 1103 76-77 0.3137377138123014 1103 78-79 0.333648926810703 1103 80-81 0.3112573465270003 1103 82-83 0.38983594143019573 1103 84-85 0.370705143397025 1103 86-87 0.31897333476066336 1103 88-89 0.29669357367648885 1103 90-91 0.2900295640076891 1103 92-93 0.21379138405788467 1103 94-95 0.21864797161737926 1103 96-97 0.22257128307138174 1103 98-99 0.16901620766884307 1103 100-101 0.17564854691188714 1104 1 0.14396778264715593 1104 2 0.1281495851481509 1104 3 0.1984587383572567 1104 4 0.12990799853673707 1104 5 0.1341503769110517 1104 6 0.13029065075549795 1104 7 0.06209738764402317 1104 8 0.10505453371293072 1104 9 0.1072531335705591 1104 10-11 0.13824608613411726 1104 12-13 0.07846632373838958 1104 14-15 0.15915825135235195 1104 16-17 0.14982645254490734 1104 18-19 0.08947672238582527 1104 20-21 0.09889310857163736 1104 22-23 0.12496654837507037 1104 24-25 0.14656220851095725 1104 26-27 0.11676905343240662 1104 28-29 0.0946447037913174 1104 30-31 0.1292928448488766 1104 32-33 0.1329554134071671 1104 34-35 0.11379763800926668 1104 36-37 0.08335255533323505 1104 38-39 0.04152897129488764 1104 40-41 0.03239375481375362 1104 42-43 0.03853320882306832 1104 44-45 0.032564034882760495 1104 46-47 0.11793675807464155 1104 48-49 0.03272380328559876 1104 50-51 0.01620660948453434 1104 52-53 0.05537574111187382 1104 54-55 0.19507880307061498 1104 56-57 0.09413378373643155 1104 58-59 0.150279021334093 1104 60-61 0.09628444792599566 1104 62-63 0.09541400559609059 1104 64-65 0.0794322497243769 1104 66-67 0.1344460446309128 1104 68-69 0.15078068637475184 1104 70-71 0.12918387203663428 1104 72-73 0.08642507824100676 1104 74-75 0.11316334770624081 1104 76-77 0.09452135227252967 1104 78-79 0.09830198469153117 1104 80-81 0.12960728814115186 1104 82-83 0.1597700405984348 1104 84-85 0.11029855504355268 1104 86-87 0.06778743097046913 1104 88-89 0.05148238671625194 1104 90-91 0.07159259087229941 1104 92-93 0.02682979383408224 1104 94-95 0.01942715242946136 1104 96-97 -0.019230958955834154 1104 98-99 0.032690049208273564 1104 100-101 -0.023486255957013924 1105 1 0.3245533938485039 1105 2 0.32225246811337627 1105 3 0.3183693066738513 1105 4 0.34002881634784643 1105 5 0.35816548487613886 1105 6 0.32738574114523544 1105 7 0.3758072074635379 1105 8 0.33771188542345953 1105 9 0.4227050168782611 1105 10-11 0.4414348583643175 1105 12-13 0.37555906973973663 1105 14-15 0.5085709924459536 1105 16-17 0.4487338181415339 1105 18-19 0.4433367427942585 1105 20-21 0.4793250795863955 1105 22-23 0.41491057477897897 1105 24-25 0.46501577997929644 1105 26-27 0.497128682745398 1105 28-29 0.5175445033183053 1105 30-31 0.521666751091864 1105 32-33 0.5015994759799156 1105 34-35 0.5500944513549584 1105 36-37 0.5693972434094405 1105 38-39 0.5086139626571011 1105 40-41 0.5125991168045374 1105 42-43 0.506813084495306 1105 44-45 0.5215094362547106 1105 46-47 0.5214577285986337 1105 48-49 0.45863996433869403 1105 50-51 0.3901350635213916 1105 52-53 0.4658416021067069 1105 54-55 0.6224725787254712 1105 56-57 0.594147555929446 1105 58-59 0.6577913355373042 1105 60-61 0.6258212190247221 1105 62-63 0.6916838558622302 1105 64-65 0.6607226742599437 1105 66-67 0.6256500655564992 1105 68-69 0.6546054799858254 1105 70-71 0.6294971148883164 1105 72-73 0.6406550999547704 1105 74-75 0.6254960467169113 1105 76-77 0.6387487652093213 1105 78-79 0.6388363493985239 1105 80-81 0.6197563677921565 1105 82-83 0.6413848362928611 1105 84-85 0.5989973333230658 1105 86-87 0.5482621028187395 1105 88-89 0.5816572046563024 1105 90-91 0.5898320330474931 1105 92-93 0.6195377817877734 1105 94-95 0.5963184986507102 1105 96-97 0.5409166635428804 1105 98-99 0.5456754114743028 1105 100-101 0.5320958996332692 1106 1 0.26614977532456763 1106 2 0.323771581485488 1106 3 0.30975834188867424 1106 4 0.3340141006997328 1106 5 0.32483438076194915 1106 6 0.3457783858825749 1106 7 0.40602141705522854 1106 8 0.36005069951769286 1106 9 0.4030851508163735 1106 10-11 0.42695454634515784 1106 12-13 0.45009953827663907 1106 14-15 0.454370055075799 1106 16-17 0.5017759543735849 1106 18-19 0.5138065007033887 1106 20-21 0.5298065693449843 1106 22-23 0.573990223650469 1106 24-25 0.5492540223681104 1106 26-27 0.5458485029152058 1106 28-29 0.620309634893502 1106 30-31 0.6113166348631935 1106 32-33 0.5500184179566645 1106 34-35 0.6153769260747666 1106 36-37 0.593412035049738 1106 38-39 0.6303400371509653 1106 40-41 0.5539223665960264 1106 42-43 0.6173946157136498 1106 44-45 0.5819095326478063 1106 46-47 0.6133752148961591 1106 48-49 0.6041352401457445 1106 50-51 0.5310565539351373 1106 52-53 0.6234282429064564 1106 54-55 0.6670088253342001 1106 56-57 0.6811421449154693 1106 58-59 0.6862879763687246 1106 60-61 0.7091256359134093 1106 62-63 0.6899868586363596 1106 64-65 0.7277265087182698 1106 66-67 0.6885603638471949 1106 68-69 0.7093607566375084 1106 70-71 0.7227978269090656 1106 72-73 0.7382244534953202 1106 74-75 0.7101983387045223 1106 76-77 0.7182746656010153 1106 78-79 0.7432494378127146 1106 80-81 0.6634142859012186 1106 82-83 0.685469242775671 1106 84-85 0.7129353608404294 1106 86-87 0.6831271037924118 1106 88-89 0.7468636918393834 1106 90-91 0.7043211134466567 1106 92-93 0.6834421861164373 1106 94-95 0.7035433509922733 1106 96-97 0.684837856793056 1106 98-99 0.702482173023288 1106 100-101 0.7520555787619507 1107 1 0.1786709773700892 1107 2 0.18698516008669586 1107 3 0.18148103681957295 1107 4 0.24879481122901836 1107 5 0.19176025478607528 1107 6 0.21492656279286138 1107 7 0.21142870689298832 1107 8 0.261221486678032 1107 9 0.303572345415013 1107 10-11 0.2776586158130314 1107 12-13 0.30783205383886525 1107 14-15 0.40770090983674834 1107 16-17 0.32318908908531796 1107 18-19 0.30435501642964624 1107 20-21 0.35344084929247543 1107 22-23 0.3906157439918161 1107 24-25 0.35287043668284923 1107 26-27 0.37586506709164524 1107 28-29 0.3413947885956503 1107 30-31 0.31997185503427517 1107 32-33 0.3619659791461345 1107 34-35 0.3642833709177822 1107 36-37 0.3573142040466877 1107 38-39 0.4591792322814001 1107 40-41 0.4090788270351595 1107 42-43 0.3857889859928605 1107 44-45 0.46880803711527363 1107 46-47 0.4234573639552792 1107 48-49 0.344399265215408 1107 50-51 0.3291234559152585 1107 52-53 0.38918968205524607 1107 54-55 0.4214569233518901 1107 56-57 0.4089809313695909 1107 58-59 0.421660725905987 1107 60-61 0.48615868932773765 1107 62-63 0.4862757896797305 1107 64-65 0.4801633595963608 1107 66-67 0.5508034712545964 1107 68-69 0.5014456480932452 1107 70-71 0.5518156700062207 1107 72-73 0.51379577390545 1107 74-75 0.516612388860473 1107 76-77 0.48862202426341383 1107 78-79 0.44623696883741815 1107 80-81 0.4112343626668249 1107 82-83 0.4114551647435043 1107 84-85 0.45528611828722987 1107 86-87 0.49260712993678624 1107 88-89 0.5365325379672932 1107 90-91 0.5632652227027215 1107 92-93 0.5132193160330374 1107 94-95 0.5369733856505228 1107 96-97 0.5403965557267512 1107 98-99 0.6145929526290601 1107 100-101 0.6220775405477035 1108 1 0.12422928739762185 1108 2 0.1485717223072669 1108 3 0.16326666332713557 1108 4 0.13173772673477657 1108 5 0.15550215506845433 1108 6 0.1649089890711437 1108 7 0.1752102552060677 1108 8 0.19038993888613476 1108 9 0.15780606831541633 1108 10-11 0.21685564528905132 1108 12-13 0.23655480665522077 1108 14-15 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-1.5203366778241048 2316 54-55 -1.4804251641593211 2316 56-57 -1.3986951085546409 2316 58-59 -1.3082569551648078 2316 60-61 -1.339489443176376 2316 62-63 -1.4747022719337295 2316 64-65 -1.3698876013661163 2316 66-67 -1.2186193539416905 2316 68-69 -1.232083733482881 2316 70-71 -1.4967546010224808 2316 72-73 -1.4643045798773642 2316 74-75 -1.2486795752156148 2316 76-77 -1.2279072836841536 2316 78-79 -1.2324214077892677 2316 80-81 -1.1920457228300805 2316 82-83 -1.1473424673911232 2316 84-85 -1.2032218756537745 2316 86-87 -1.0515958518250272 2316 88-89 -1.0398799053576049 2316 90-91 -1.071280635337022 2316 92-93 -1.2049856878324618 2316 94-95 -1.519368234065741 2316 96-97 -1.2880053704159984 2316 98-99 -1.1166664017947134 2316 100-101 -1.0405543965978836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96977.0 3 20190.0 4 16248.0 5 14640.0 6 15145.0 7 16021.0 8 17421.0 9 21177.0 10 26154.0 11 31121.0 12 38124.0 13 46425.0 14 52539.0 15 61269.0 16 73884.0 17 91554.0 18 73929.0 19 47835.0 20 43942.0 21 45525.0 22 48403.0 23 52611.0 24 61005.0 25 72131.0 26 87342.0 27 105709.0 28 130741.0 29 162247.0 30 204831.0 31 263160.0 32 348411.0 33 478745.0 34 686328.0 35 1039771.0 36 1627739.0 37 2474735.0 38 2687523.0 39 668622.0 40 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9542034809179 21.13957467546911 14.758909950687107 22.147311892925885 2 15.299655392046008 21.24818204262682 40.63089189351801 22.82127067180916 3 17.90689960424004 26.780905297693003 30.99981651423351 24.312378583833453 4 12.30030857827351 17.536495897559615 39.75642208873707 30.406773435429812 5 12.429946918494279 38.28869985663247 35.722200258453256 13.559152966419996 6 28.99600733302959 38.97076995980776 18.322656621511605 13.710566085651047 7 25.674691962383715 33.60040244594456 23.479147514330332 17.245758077341385 8 24.650011390008412 35.71868463452596 21.300145601236842 18.33115837422879 9 24.937925473387253 16.886271367140097 22.534161115411063 35.641642044061584 10-11 23.080486563663214 27.488870136712002 29.612731215834863 19.81791208378992 12-13 23.99784803366462 25.287919900045193 29.706878615571092 21.007353450719098 14-15 21.350022740515755 27.087433496802532 27.668475354745613 23.894068407936096 16-17 20.16689680223662 30.424771602704997 28.24442916247071 21.163902432587676 18-19 20.076306157575548 28.871294344515302 31.077068356081515 19.975331141827635 20-21 21.207794048549864 28.064921470487143 30.794251697084256 19.93303278387874 22-23 20.827661820627462 27.941389119898652 30.931134692986056 20.29981436648783 24-25 20.60041640256813 28.16544066159667 30.774124947742205 20.460017988093 26-27 20.31139649307587 28.24578567587252 30.822160880581123 20.620656950470487 28-29 20.396444964187495 28.191265699998812 30.96443943474403 20.447849901069663 30-31 20.68000177089197 28.538600445154444 30.52620677387755 20.255191010076036 32-33 20.330980812485226 28.36252034798034 30.8940265234098 20.41247231612463 34-35 20.62355273168575 28.16305718170387 30.740389773256872 20.473000313353506 36-37 20.927261012023767 28.312567459198974 30.314270398771754 20.445901130005506 38-39 20.690703938479135 28.47629508544445 30.421406595660883 20.411594380415536 40-41 20.609773666376324 28.320129930052474 30.481745660810265 20.588350742760937 42-43 20.63321684534051 28.363104285093428 30.295413284245225 20.708265585320838 44-45 20.74237402437414 28.564298502527848 30.042765830789193 20.65056164230882 46-47 21.00901763306968 28.652775479261983 29.670525074700148 20.66768181296819 48-49 20.99600526678056 28.58481994737535 29.51596723150282 20.903207554341268 50-51 20.946269069306673 28.715010342263376 29.300458508600354 21.0382620798296 52-53 20.854136458096363 28.894512191225218 29.265762742417937 20.985588608260475 54-55 20.944336592999406 29.174842665618545 29.1846812513984 20.696139489983647 56-57 20.909372315837956 28.87141333709548 29.19048784839645 21.028726498670107 58-59 20.86757418655584 28.57159810750318 29.455683092262124 21.10514461367886 60-61 21.053563197472798 28.792302089846377 29.22683770920735 20.927297003473473 62-63 20.81186292871291 28.851854591929776 29.344082466934296 20.99220001242302 64-65 20.89266054752756 28.7681403777996 29.269247224398185 21.069951850274656 66-67 20.789836322708354 28.71390865491879 29.219870980623526 21.27638404174933 68-69 21.047759451332478 28.980393409782423 29.047176062640844 20.924671076244255 70-71 21.09046009138999 28.706018820975594 29.037357018754545 21.166164068879873 72-73 20.874698037315163 28.81214456367585 29.159989809407712 21.153167589601278 74-75 20.947049848653215 28.954344767441786 29.01708176610128 21.081523617803715 76-77 21.03996435382004 28.9822647142156 28.859131362546847 21.118639569417518 78-79 21.02679957982435 28.971555154070977 29.02334887795134 20.978296388153336 80-81 20.996856636106404 28.97284425569534 28.96354475539098 21.066754352807273 82-83 20.829139939747925 28.94283157522039 29.17730473144271 21.050723753588972 84-85 20.798044638886186 28.84016558641787 29.00700599417123 21.354783780524716 86-87 20.939427143735816 28.9469797930034 28.907186639392656 21.206406423868124 88-89 20.780143491930186 28.850778267997384 29.056559853379476 21.312518386692954 90-91 20.684117843785035 28.68310790120322 29.12875647603897 21.50401777897278 92-93 20.781811470603515 28.84200970823673 28.996705997633605 21.379472823526154 94-95 20.711333042940588 28.59318076068681 29.15147173770183 21.544014458670777 96-97 20.713896422717237 28.705430227644136 29.07750879595926 21.503164553679365 98-99 20.628737661083985 28.692050109166335 29.01339336897258 21.665818860777105 100-101 20.655631568957617 28.637333411014897 28.97462161567528 21.73241340435221 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4225.0 1 2750.0 2 1662.0 3 2178.0 4 2019.0 5 1953.0 6 3229.5 7 5941.5 8 9380.5 9 11511.5 10 10836.5 11 9932.5 12 10431.0 13 11334.5 14 12298.5 15 13152.0 16 13980.0 17 15113.0 18 16918.5 19 19285.0 20 22646.0 21 27830.5 22 36249.0 23 49576.5 24 65606.0 25 83218.5 26 107237.5 27 138381.0 28 178570.5 29 220528.5 30 253188.5 31 286192.5 32 324693.5 33 358417.0 34 384979.0 35 408973.5 36 425880.0 37 437459.0 38 449357.0 39 454385.5 40 453278.0 41 445735.5 42 434539.0 43 422702.0 44 406669.0 45 393540.5 46 382613.5 47 367039.0 48 351667.0 49 341253.5 50 330807.0 51 313493.5 52 288581.5 53 267913.5 54 253672.5 55 240970.0 56 219839.5 57 192973.5 58 173833.5 59 157219.0 60 136943.0 61 115476.0 62 96430.5 63 79496.0 64 65251.5 65 52820.5 66 41559.5 67 32368.0 68 25193.0 69 19369.0 70 14330.5 71 10563.5 72 8057.0 73 6081.0 74 4515.5 75 3378.5 76 2448.5 77 1796.5 78 1413.5 79 1068.0 80 781.5 81 603.5 82 482.0 83 371.0 84 310.5 85 269.5 86 227.0 87 200.0 88 180.0 89 154.5 90 123.5 91 110.5 92 93.5 93 67.0 94 53.5 95 44.5 96 45.5 97 35.5 98 20.0 99 14.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014223488007541604 2 0.004688606023489501 3 0.004223894630010896 4 0.005900175013630151 5 0.0028380588673157688 6 0.007468575966620445 7 0.0014605215223613313 8 0.003775780072013669 9 0.01048920002423138 10-11 0.007684334827878368 12-13 0.008671846539020405 14-15 0.003414798900293681 16-17 0.005879428969278427 18-19 0.003979091306660559 20-21 9.626164579199683E-4 22-23 0.0011078387683820326 24-25 0.007074401123937698 26-27 0.001074645097419275 28-29 7.551560144027338E-4 30-31 5.435463620151546E-4 32-33 0.001277956332066165 34-35 0.0016762803836192551 36-37 0.001248911869973752 38-39 0.0016928772191006339 40-41 0.002605703170576466 42-43 0.002663792094761292 44-45 0.007609649068212164 46-47 0.013708986107618861 48-49 0.005178212670190175 50-51 0.005489403335466027 52-53 0.01343513832217611 54-55 0.008248627234245246 56-57 0.005310987354041205 58-59 0.006638734192551505 60-61 0.0012821055409365095 62-63 0.002311109340781993 64-65 0.002203229910153031 66-67 0.002360899847226129 68-69 0.0017011756368413235 70-71 0.0021949314924123416 72-73 0.0013277468385103012 74-75 0.001531058073157191 76-77 0.007900093689136292 78-79 0.006369035615979101 80-81 0.012078347021573397 82-83 0.014737989907464343 84-85 0.007891795271395602 86-87 0.0056304764370577465 88-89 0.0027799699431309433 90-91 0.002489525322206815 92-93 8.630354450316957E-4 94-95 0.0011866737369185818 96-97 0.001763413769896494 98-99 0.006294349856312897 100-101 0.010779644645155509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.205049E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.35971925519454 #Duplication Level Percentage of deduplicated Percentage of total 1 66.49524987374474 31.491963658245613 2 17.538968502653628 16.61281248622751 3 6.909893158715437 9.817518002404576 4 3.1453864634624935 5.958584794346916 5 1.7638106342088258 4.176678822772831 6 1.0455518160413086 2.9710224284685114 7 0.6674065942642016 2.2125732253392685 8 0.4425880389483414 1.6768676216240446 9 0.3236142299162096 1.3793651168235899 >10 1.515790341082342 12.589518944666212 >50 0.09180770374229522 2.9973267330770823 >100 0.053925620573173266 4.786435563620518 >500 0.004531253953839149 1.470159186075577 >1k 0.0014054952733919348 1.1263858788704264 >5k 1.7568354924029435E-5 0.06372535822091813 >10k+ 5.2705064772088305E-5 0.6690621792164367 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35713 0.2963613927732399 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28334 0.23512736826469294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16366 0.13581190474412244 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.298417740689382E-6 0.0 0.0 0.0 3 0.0 8.298417740689382E-6 0.0 8.298417740689382E-6 0.0 4 0.0 8.298417740689382E-6 0.0 3.319367096275753E-5 0.0 5 0.0 1.6596835481378764E-5 0.0 4.149208870344691E-5 0.0 6 0.0 1.6596835481378764E-5 0.0 4.149208870344691E-5 0.0 7 0.0 1.6596835481378764E-5 0.0 4.97905064441363E-5 0.0 8 0.0 1.6596835481378764E-5 0.0 6.638734192551506E-5 0.0 9 4.149208870344691E-5 1.6596835481378764E-5 0.0 1.2447626611034073E-4 0.0 10-11 4.149208870344691E-5 1.6596835481378764E-5 0.0 1.6181914594344296E-4 0.0 12-13 4.149208870344691E-5 1.6596835481378764E-5 0.0 2.1160965238757926E-4 0.0 14-15 4.149208870344691E-5 2.0746044351723456E-5 0.0 2.738477854427496E-4 0.0 16-17 4.149208870344691E-5 2.489525322206815E-5 0.0 3.1948908301654124E-4 0.0 18-19 4.149208870344691E-5 3.734287983310222E-5 0.0 3.6098117171998813E-4 0.0 20-21 4.149208870344691E-5 4.149208870344691E-5 0.0 4.0662246929377976E-4 4.149208870344691E-6 22-23 4.149208870344691E-5 4.97905064441363E-5 0.0 5.352479442744652E-4 8.298417740689382E-6 24-25 4.149208870344691E-5 6.223813305517037E-5 0.0 7.178131345696316E-4 8.298417740689382E-6 26-27 4.149208870344691E-5 6.638734192551506E-5 0.0 9.79213293401347E-4 8.298417740689382E-6 28-29 4.149208870344691E-5 8.298417740689383E-5 0.0 0.0019003376626178686 8.298417740689382E-6 30-31 4.149208870344691E-5 9.12825951475832E-5 0.0 0.004373266149343305 8.298417740689382E-6 32-33 4.149208870344691E-5 9.12825951475832E-5 0.0 0.010107472808159667 8.298417740689382E-6 34-35 5.393971531448099E-5 9.543180401792791E-5 0.0 0.021206606536331718 8.298417740689382E-6 36-37 5.808892418482568E-5 1.1202863949930666E-4 4.149208870344691E-6 0.0390025633812401 8.298417740689382E-6 38-39 5.808892418482568E-5 1.2862547498068542E-4 8.298417740689382E-6 0.06290615568329587 8.298417740689382E-6 40-41 5.808892418482568E-5 1.327746838510301E-4 8.298417740689382E-6 0.08715413232159025 8.298417740689382E-6 42-43 5.808892418482568E-5 1.327746838510301E-4 8.298417740689382E-6 0.11177553775821564 8.298417740689382E-6 44-45 5.808892418482568E-5 1.369238927213748E-4 8.298417740689382E-6 0.14408542723158974 8.298417740689382E-6 46-47 6.223813305517037E-5 1.493715193324089E-4 8.298417740689382E-6 0.18397592131108362 8.298417740689382E-6 48-49 6.638734192551506E-5 1.493715193324089E-4 8.298417740689382E-6 0.22529374324197604 8.298417740689382E-6 50-51 6.638734192551506E-5 1.493715193324089E-4 8.298417740689382E-6 0.26695594950910706 8.298417740689382E-6 52-53 6.638734192551506E-5 1.5352072820275358E-4 8.298417740689382E-6 0.308663797073812 8.298417740689382E-6 54-55 6.638734192551506E-5 1.5766993707309827E-4 8.298417740689382E-6 0.35671993421014414 8.298417740689382E-6 56-57 6.638734192551506E-5 1.5766993707309827E-4 8.298417740689382E-6 0.4156138049158167 8.298417740689382E-6 58-59 6.638734192551506E-5 1.5766993707309827E-4 8.298417740689382E-6 0.48355295095884065 8.298417740689382E-6 60-61 6.638734192551506E-5 1.5766993707309827E-4 8.298417740689382E-6 0.5548778514400659 8.298417740689382E-6 62-63 6.638734192551506E-5 1.6181914594344296E-4 8.298417740689382E-6 0.6264600028712526 8.298417740689382E-6 64-65 6.638734192551506E-5 1.7011756368413234E-4 8.298417740689382E-6 0.7054360445093935 8.298417740689382E-6 66-67 6.638734192551506E-5 1.7426677255447703E-4 8.298417740689382E-6 0.8009010421982841 8.298417740689382E-6 68-69 6.638734192551506E-5 1.7426677255447703E-4 8.298417740689382E-6 0.9105770802681052 8.298417740689382E-6 70-71 6.638734192551506E-5 1.8256519029516644E-4 8.298417740689382E-6 1.0302029212090131 1.2447626611034073E-5 72-73 7.053655079585974E-5 1.9086360803585581E-4 8.298417740689382E-6 1.1558160705498284 1.6596835481378764E-5 74-75 8.298417740689383E-5 1.9086360803585581E-4 8.298417740689382E-6 1.2870389502833495 1.6596835481378764E-5 76-77 8.298417740689383E-5 1.9086360803585581E-4 8.298417740689382E-6 1.4333898455581475 1.6596835481378764E-5 78-79 8.298417740689383E-5 1.9086360803585581E-4 8.298417740689382E-6 1.604038507977684 1.6596835481378764E-5 80-81 8.298417740689383E-5 1.9086360803585581E-4 8.298417740689382E-6 1.794267287056377 1.6596835481378764E-5 82-83 9.12825951475832E-5 1.9086360803585581E-4 8.298417740689382E-6 1.9961138509720353 1.6596835481378764E-5 84-85 9.12825951475832E-5 1.9086360803585581E-4 8.298417740689382E-6 2.204632342751208 1.6596835481378764E-5 86-87 9.12825951475832E-5 1.9086360803585581E-4 8.298417740689382E-6 2.431195743907509 1.6596835481378764E-5 88-89 9.95810128882726E-5 1.9086360803585581E-4 8.298417740689382E-6 2.6868907405425007 1.6596835481378764E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20365 0.0 54.41792 1 GTATCAA 38465 0.0 42.123802 1 ATCAACG 46120 0.0 34.749565 3 TCAACGC 46570 0.0 34.374126 4 CAACGCA 48710 0.0 32.85352 5 TATCAAC 48945 0.0 32.84942 2 AACGCAG 51420 0.0 31.223646 6 ACGCAGA 59060 0.0 26.968523 7 CGCAGAG 58765 0.0 26.950203 8 GTACATG 40525 0.0 26.479197 1 GTGGTAT 9460 0.0 26.211445 1 TACATGG 39635 0.0 25.537712 2 GCAGAGT 63405 0.0 24.221298 9 ACATGGG 40995 0.0 24.065739 3 TGGTATC 9605 0.0 23.885103 2 CATGGGT 13140 0.0 21.97942 4 GAGTACT 40415 0.0 21.07359 12-13 CAGAGTA 61615 0.0 20.491377 10-11 AGAGTAC 58305 0.0 20.367456 10-11 GTACTTT 43940 0.0 19.187021 14-15 >>END_MODULE