FastQCFastQC Report
Wed 25 May 2016
SRR2049421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12050490
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344500.28588049116674924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286920.23809820181585978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190820.15835040732783479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194550.055.3020971
GTATCAA374950.042.154631
ATCAACG473800.032.9423373
TCAACGC477550.032.683654
CAACGCA497450.031.4524335
TATCAAC500000.031.4065442
AACGCAG529900.029.6873256
GTGGTAT94050.025.7190761
CGCAGAG611350.025.41268
ACGCAGA618150.025.2713497
TGGTATC90000.024.6468642
GTACATG389650.024.111731
TACATGG382550.023.3802622
GCAGAGT663750.022.8770549
ACATGGG396950.021.9454963
AGAGTAC612550.020.7803810-11
CATGGGT128950.020.5539234
GAGTACT430850.020.01383812-13
AGTACTT452250.019.24009112-13
CAGAGTA649600.019.236910-11
Produced by FastQC (version 0.11.5)