FastQCFastQC Report
Wed 25 May 2016
SRR2049420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13553994
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355030.26193755139628955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297340.2193744515454264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170400.1257194004955292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA201950.053.2306141
GTATCAA377450.040.9775241
ATCAACG447950.034.0067063
TCAACGC451200.033.7622534
CAACGCA473100.032.2181555
TATCAAC480000.031.8537392
AACGCAG496450.030.8563486
GTGGTAT98600.028.3765221
ACGCAGA569750.026.6050977
CGCAGAG573300.026.3243488
TGGTATC102650.025.5423492
GTACATG411650.024.1871051
GCAGAGT631900.023.161459
TACATGG409850.022.761312
ACATGGG427150.021.02843
GAGTACT389050.020.83642612-13
CAGAGTA599350.020.24632310-11
AGAGTAC566750.020.16623710-11
AGTACTT413250.018.5127212-13
GAGTACA303100.018.336681
Produced by FastQC (version 0.11.5)