##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13553994 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.523467178751886 31.0 31.0 34.0 30.0 34.0 2 31.57586110780335 33.0 31.0 34.0 30.0 34.0 3 31.607770595147084 33.0 31.0 34.0 30.0 34.0 4 35.232933185598284 37.0 35.0 37.0 33.0 37.0 5 35.24578563337124 37.0 35.0 37.0 33.0 37.0 6 35.3378370980539 37.0 35.0 37.0 33.0 37.0 7 35.28436186411179 37.0 35.0 37.0 33.0 37.0 8 35.25638428053015 37.0 35.0 37.0 33.0 37.0 9 36.853393103169445 39.0 37.0 39.0 33.0 39.0 10-11 36.78566148103651 39.0 37.0 39.0 33.0 39.0 12-13 36.744128852351565 39.0 37.0 39.0 33.0 39.0 14-15 37.94151015560432 40.0 38.0 41.0 33.0 41.0 16-17 37.92534849875247 40.0 38.0 41.0 33.0 41.0 18-19 37.92849672207321 40.0 38.0 41.0 33.0 41.0 20-21 37.882841065150245 40.0 38.0 41.0 33.0 41.0 22-23 37.78192457514737 40.0 38.0 41.0 33.0 41.0 24-25 37.67544747326876 40.0 38.0 41.0 32.5 41.0 26-27 37.53618287716521 40.0 37.0 41.0 32.0 41.0 28-29 37.3836684596437 40.0 37.0 41.0 32.0 41.0 30-31 37.21896630616776 40.0 37.0 41.0 32.0 41.0 32-33 37.00093101708618 40.0 36.5 41.0 31.0 41.0 34-35 36.81775670698984 39.5 36.0 41.0 31.0 41.0 36-37 36.6435301284625 39.0 36.0 41.0 30.0 41.0 38-39 36.4536853860198 39.0 36.0 40.5 30.0 41.0 40-41 36.25319592143836 39.0 35.0 40.0 30.0 41.0 42-43 36.036043139756444 39.0 35.0 40.0 29.5 41.0 44-45 35.78515768119715 39.0 35.0 40.0 28.5 41.0 46-47 35.47276367393995 38.5 35.0 40.0 27.5 41.0 48-49 35.21139399943662 38.0 35.0 40.0 27.0 41.0 50-51 33.87879528351569 37.0 32.5 39.0 25.0 40.0 52-53 34.210309300712396 37.5 33.5 39.0 26.0 40.0 54-55 35.27917697174722 39.0 35.0 40.0 28.0 41.0 56-57 35.40465301224127 39.0 35.0 40.0 28.0 41.0 58-59 35.25087332191529 39.0 35.0 40.0 27.5 41.0 60-61 34.996660836650804 38.0 34.5 40.0 26.5 41.0 62-63 34.725299273409746 38.0 34.0 40.0 26.0 41.0 64-65 34.44667254537666 37.5 34.0 40.0 26.0 41.0 66-67 34.12070862654949 37.0 34.0 40.0 26.0 41.0 68-69 33.77287373743857 36.5 34.0 39.5 25.0 41.0 70-71 33.428076845835996 36.0 33.0 39.0 24.5 41.0 72-73 33.1611979834136 36.0 33.0 39.0 24.0 40.0 74-75 32.9550232573513 35.0 33.0 38.5 24.0 40.0 76-77 32.605832900619546 35.0 33.0 37.5 23.5 39.5 78-79 32.223498512689325 35.0 33.0 37.0 22.0 39.0 80-81 31.87298802847338 35.0 32.5 36.5 21.0 39.0 82-83 31.523818698754035 35.0 32.0 36.0 20.0 38.5 84-85 31.20201879239433 35.0 32.0 36.0 19.0 37.0 86-87 30.90493130659494 35.0 32.0 35.0 18.0 37.0 88-89 30.614244406482694 35.0 31.5 35.0 16.0 36.5 90-91 30.34691482820488 34.5 31.0 35.0 9.0 36.0 92-93 30.0918030139308 34.0 31.0 35.0 4.5 36.0 94-95 29.830558763712013 34.0 31.0 35.0 2.0 36.0 96-97 29.567554589444264 34.0 31.0 35.0 2.0 35.0 98-99 29.241993282570437 34.0 31.0 35.0 2.0 35.0 100-101 28.68296724935838 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3816632214215403 1101 2 0.43812581849483934 1101 3 0.42413850252291496 1101 4 0.40459792666684535 1101 5 0.3633648621451755 1101 6 0.4164552661231937 1101 7 0.4172136035641074 1101 8 0.4193477617771322 1101 9 0.46886238134701586 1101 10-11 0.46501480280074503 1101 12-13 0.4642892579408979 1101 14-15 0.5816568709548804 1101 16-17 0.5483462060755713 1101 18-19 0.5267010310202309 1101 20-21 0.48695993933352355 1101 22-23 0.5196517828103993 1101 24-25 0.5228125407768829 1101 26-27 0.5419485868932767 1101 28-29 0.47939004814790565 1101 30-31 0.4489608628386108 1101 32-33 0.4303945826142339 1101 34-35 0.4316665420081449 1101 36-37 0.4445955598995113 1101 38-39 0.4087641380012741 1101 40-41 0.3516387647167889 1101 42-43 0.3319116715500172 1101 44-45 0.253164729889356 1101 46-47 0.24219732758552226 1101 48-49 0.15402125230126984 1101 50-51 0.05536312890453132 1101 52-53 0.19595170663792771 1101 54-55 0.39645787065152405 1101 56-57 0.41163469815575127 1101 58-59 0.3997153571677927 1101 60-61 0.4531944723364134 1101 62-63 0.38064022615833437 1101 64-65 0.3579515861617537 1101 66-67 0.2988970712064969 1101 68-69 0.24956941233797636 1101 70-71 0.16017732023596665 1101 72-73 0.18747635305145138 1101 74-75 0.16907596187535034 1101 76-77 0.1506975897210694 1101 78-79 0.11814411008850811 1101 80-81 -0.011925777853925013 1101 82-83 -0.0017940295141016804 1101 84-85 -0.04359934686948819 1101 86-87 -0.12892848547928537 1101 88-89 -0.17396367389688194 1101 90-91 -0.18634571740924244 1101 92-93 -0.19185852340809362 1101 94-95 -0.3438517796939422 1101 96-97 -0.39610698435200575 1101 98-99 -0.4907802088333497 1101 100-101 -0.5766015123007726 1102 1 0.3084552967954579 1102 2 0.2975418224314552 1102 3 0.346174609817524 1102 4 0.32684878662672645 1102 5 0.31645819096865324 1102 6 0.32959097512560476 1102 7 0.32041230272414367 1102 8 0.3511301065125352 1102 9 0.41051531412092146 1102 10-11 0.4044487735972737 1102 12-13 0.38529474581248024 1102 14-15 0.47101072651165765 1102 16-17 0.39743625263572113 1102 18-19 0.40141208500607206 1102 20-21 0.3948976323077886 1102 22-23 0.3968159854442419 1102 24-25 0.4128109305727037 1102 26-27 0.42102850108672385 1102 28-29 0.43681637551750896 1102 30-31 0.33967945107064423 1102 32-33 0.39472327035531407 1102 34-35 0.3998768409742155 1102 36-37 0.3777447590932965 1102 38-39 0.3069871763648564 1102 40-41 0.3429414727926954 1102 42-43 0.33049439388523894 1102 44-45 0.3101182499199382 1102 46-47 0.26207922901871683 1102 48-49 0.19463663077149107 1102 50-51 0.1243089319452011 1102 52-53 0.33665187767480376 1102 54-55 0.4790730352507069 1102 56-57 0.5031342124907567 1102 58-59 0.5181560835454917 1102 60-61 0.5304485198493367 1102 62-63 0.4833155983816937 1102 64-65 0.42028347378690256 1102 66-67 0.39684526636075645 1102 68-69 0.3979302343611977 1102 70-71 0.30562166611709074 1102 72-73 0.3765332106915622 1102 74-75 0.36431190457131635 1102 76-77 0.3083716643653034 1102 78-79 0.30493452424141765 1102 80-81 0.24450187027572312 1102 82-83 0.1508164567002055 1102 84-85 0.15761263035062356 1102 86-87 0.12493164048739658 1102 88-89 0.12185158083517322 1102 90-91 0.02994725661917741 1102 92-93 -0.04670160273396817 1102 94-95 -0.13283126630635778 1102 96-97 -0.17279142537035241 1102 98-99 -0.30419732681778555 1102 100-101 -0.3542841435497799 1103 1 0.285947396531661 1103 2 0.2682862702590967 1103 3 0.30949232539021665 1103 4 0.2962603123002836 1103 5 0.309083783871138 1103 6 0.334286612487773 1103 7 0.31656123793501934 1103 8 0.3012410414128013 1103 9 0.39526804156975004 1103 10-11 0.4227810149676614 1103 12-13 0.3863986173731462 1103 14-15 0.43829004655180626 1103 16-17 0.47734913124859446 1103 18-19 0.4042620526368452 1103 20-21 0.4149211285300751 1103 22-23 0.39520248911617983 1103 24-25 0.4652977560443716 1103 26-27 0.47431713166499634 1103 28-29 0.40975067571584134 1103 30-31 0.3910042628253265 1103 32-33 0.3418762418397776 1103 34-35 0.39389976050647135 1103 36-37 0.3925751949674279 1103 38-39 0.37134909611921785 1103 40-41 0.3070769526449979 1103 42-43 0.2783222430039771 1103 44-45 0.2976737577272459 1103 46-47 0.21310934437342155 1103 48-49 0.24287060660950743 1103 50-51 0.2075521175675874 1103 52-53 0.3328319073888153 1103 54-55 0.4260911414251325 1103 56-57 0.4709922153730943 1103 58-59 0.4476408421734419 1103 60-61 0.49368900571625574 1103 62-63 0.4325759154832838 1103 64-65 0.38928530461075894 1103 66-67 0.4982140535137063 1103 68-69 0.41331821148731507 1103 70-71 0.37374023158393044 1103 72-73 0.45305296983997323 1103 74-75 0.4342961290929992 1103 76-77 0.32802575054261496 1103 78-79 0.34121674944110225 1103 80-81 0.34446243714643643 1103 82-83 0.3133347997294642 1103 84-85 0.32121623611062233 1103 86-87 0.2542546364477829 1103 88-89 0.19605979822282293 1103 90-91 0.22832907885948472 1103 92-93 0.18964712551421314 1103 94-95 0.1564208568982366 1103 96-97 0.12279461360965982 1103 98-99 0.12508256768578718 1103 100-101 0.1361145797163772 1104 1 0.12376154211566259 1104 2 0.1414014292688286 1104 3 0.17614064528408946 1104 4 0.09988292115285446 1104 5 0.14108794346041975 1104 6 0.10701588234600479 1104 7 0.07841515430808954 1104 8 0.12770939783584367 1104 9 0.14508592406460252 1104 10-11 0.16128251696630258 1104 12-13 0.04468670198686908 1104 14-15 0.1192429398534145 1104 16-17 0.1586522867869391 1104 18-19 0.1125346552982549 1104 20-21 0.1505297299277899 1104 22-23 0.15231734032931143 1104 24-25 0.14662193170627802 1104 26-27 0.11575394029109276 1104 28-29 0.08511186120962577 1104 30-31 0.1237014416364488 1104 32-33 0.09118030451106307 1104 34-35 0.07752898590217683 1104 36-37 0.06161995525862807 1104 38-39 0.038728048196432496 1104 40-41 -0.01876238511543704 1104 42-43 -0.06610332481437098 1104 44-45 -0.02534124004150584 1104 46-47 -0.03332172151591095 1104 48-49 -0.0919400131319037 1104 50-51 -0.23865153106850556 1104 52-53 -0.1563153849358585 1104 54-55 -0.0013797323731097322 1104 56-57 -0.10140451764776515 1104 58-59 -0.0072842583955718965 1104 60-61 -0.055750807178093 1104 62-63 7.540440961975037E-4 1104 64-65 -0.0030780503613598853 1104 66-67 0.03220259621872401 1104 68-69 -0.0027784453159611644 1104 70-71 -0.014161264833184362 1104 72-73 -0.07538108514452802 1104 74-75 -0.03314379705749104 1104 76-77 -0.04181397846632251 1104 78-79 -0.11726778310229236 1104 80-81 -0.13219207390267584 1104 82-83 -0.16854609761684713 1104 84-85 -0.1339216288593299 1104 86-87 -0.14548104322049227 1104 88-89 -0.18147108236537335 1104 90-91 -0.23127218635795188 1104 92-93 -0.24518799873834496 1104 94-95 -0.2408259837836937 1104 96-97 -0.3002579158362586 1104 98-99 -0.29129166421131103 1104 100-101 -0.3325935876501944 1105 1 0.28744753642576626 1105 2 0.2633535514040126 1105 3 0.2460755307072482 1105 4 0.23635549308041703 1105 5 0.24888059428558762 1105 6 0.2970802185354202 1105 7 0.26958838794900686 1105 8 0.26904618212515174 1105 9 0.3905034595891692 1105 10-11 0.39043858059910974 1105 12-13 0.27513867301404815 1105 14-15 0.4119655925347345 1105 16-17 0.3767282653832851 1105 18-19 0.3869669183121047 1105 20-21 0.3721084388151965 1105 22-23 0.2961969514213223 1105 24-25 0.350868444798472 1105 26-27 0.41440700702726474 1105 28-29 0.43490453283381214 1105 30-31 0.4131194352646048 1105 32-33 0.3776701012805148 1105 34-35 0.4176085379375891 1105 36-37 0.4061705525948227 1105 38-39 0.3206608435285432 1105 40-41 0.32655451415880776 1105 42-43 0.34440507614990423 1105 44-45 0.3262308688348341 1105 46-47 0.35920862167520795 1105 48-49 0.22977435418776082 1105 50-51 0.29445792396469983 1105 52-53 0.4316846173751401 1105 54-55 0.5534956043109602 1105 56-57 0.5568591100935407 1105 58-59 0.6062502156062308 1105 60-61 0.5483027924120591 1105 62-63 0.5820272138589218 1105 64-65 0.6090873940479682 1105 66-67 0.5393247335046283 1105 68-69 0.5481803762033834 1105 70-71 0.5094666251139657 1105 72-73 0.5633542700632859 1105 74-75 0.573725466734011 1105 76-77 0.5399269905850517 1105 78-79 0.5483217597615919 1105 80-81 0.5338159152305728 1105 82-83 0.5409597146052221 1105 84-85 0.5362027482075042 1105 86-87 0.4617699324161961 1105 88-89 0.48405453503714213 1105 90-91 0.4682809341720322 1105 92-93 0.4966193948077233 1105 94-95 0.44588403307506397 1105 96-97 0.37760683877053935 1105 98-99 0.4450891136739976 1105 100-101 0.44423569633526583 1106 1 0.29196876366857794 1106 2 0.35794759999111037 1106 3 0.3065495162905627 1106 4 0.30217611176067294 1106 5 0.34489015290205316 1106 6 0.35651419564040765 1106 7 0.37527451141659185 1106 8 0.3996567307567034 1106 9 0.4697722437484302 1106 10-11 0.4744177891231445 1106 12-13 0.4537737659636676 1106 14-15 0.5395553237130102 1106 16-17 0.5344224351943936 1106 18-19 0.5248149949093701 1106 20-21 0.5314630997300114 1106 22-23 0.5644962353271765 1106 24-25 0.5656576140595035 1106 26-27 0.5388826120011245 1106 28-29 0.5750099086163516 1106 30-31 0.6091390096361451 1106 32-33 0.6029384748740299 1106 34-35 0.6122724924071221 1106 36-37 0.6036744458282683 1106 38-39 0.6126957153294654 1106 40-41 0.6428778999404443 1106 42-43 0.6396085851157451 1106 44-45 0.6194347352733516 1106 46-47 0.6702470497807127 1106 48-49 0.6061954038016282 1106 50-51 0.5649554319537629 1106 52-53 0.7007602038026448 1106 54-55 0.6858942718300085 1106 56-57 0.6867404776014325 1106 58-59 0.7513474245767497 1106 60-61 0.7215144199611316 1106 62-63 0.6946820207952129 1106 64-65 0.7374124513817648 1106 66-67 0.7082231644852328 1106 68-69 0.7147954168402819 1106 70-71 0.7302567064788406 1106 72-73 0.7420572791987041 1106 74-75 0.7148387778096463 1106 76-77 0.7272828126133462 1106 78-79 0.7135162967221902 1106 80-81 0.7221607329169224 1106 82-83 0.7294215026495934 1106 84-85 0.7208021340458508 1106 86-87 0.7118583147923161 1106 88-89 0.7664513991510589 1106 90-91 0.7397933478608536 1106 92-93 0.6881820823605622 1106 94-95 0.735565792353956 1106 96-97 0.7813228753735046 1106 98-99 0.8220489959046553 1106 100-101 0.8313024224289229 1107 1 0.20560271937139873 1107 2 0.17014668524711496 1107 3 0.18937710796544138 1107 4 0.21526933980363339 1107 5 0.21323171380662131 1107 6 0.2385446789905501 1107 7 0.25543713670791846 1107 8 0.29859365805440774 1107 9 0.3589473522154307 1107 10-11 0.3063956601544433 1107 12-13 0.29651139701277174 1107 14-15 0.4026133687140927 1107 16-17 0.3457202288677763 1107 18-19 0.27803661652990996 1107 20-21 0.3714866484968198 1107 22-23 0.39015334849960226 1107 24-25 0.3523382078814379 1107 26-27 0.3675148642681805 1107 28-29 0.41314609986478956 1107 30-31 0.40995357742278316 1107 32-33 0.44139276489095636 1107 34-35 0.4509027730957058 1107 36-37 0.39991539173517765 1107 38-39 0.445114165480625 1107 40-41 0.4026677178016058 1107 42-43 0.42098979582693374 1107 44-45 0.4828008048252528 1107 46-47 0.5095535909438382 1107 48-49 0.45369457799247925 1107 50-51 0.4902117924953444 1107 52-53 0.5516100124855825 1107 54-55 0.5957955022378627 1107 56-57 0.6112609876018524 1107 58-59 0.59416912817953 1107 60-61 0.653434552267683 1107 62-63 0.694875491052052 1107 64-65 0.6760129244599753 1107 66-67 0.7206003885731604 1107 68-69 0.7104499904546131 1107 70-71 0.6935178029340534 1107 72-73 0.6545012195102373 1107 74-75 0.7010505645789351 1107 76-77 0.6679042718591219 1107 78-79 0.6648715795916331 1107 80-81 0.6897941555540221 1107 82-83 0.6274512950668765 1107 84-85 0.6765489809540632 1107 86-87 0.6912986551494456 1107 88-89 0.7116916721354407 1107 90-91 0.6903349911300118 1107 92-93 0.7119499086950363 1107 94-95 0.7032069322694205 1107 96-97 0.6799578528359334 1107 98-99 0.7498794360299961 1107 100-101 0.7702483191761651 1108 1 0.13965607988162176 1108 2 0.14800010816338371 1108 3 0.15523120266780666 1108 4 0.1823110965882364 1108 5 0.18080636076336276 1108 6 0.2093518598666222 1108 7 0.21951314589757942 1108 8 0.24249886004410826 1108 9 0.22920154088004097 1108 10-11 0.2483175897898633 1108 12-13 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-1.334387621869631 2316 62-63 -1.515946876939637 2316 64-65 -1.4229915776992499 2316 66-67 -1.3669402495413152 2316 68-69 -1.312350644575595 2316 70-71 -1.525994244801499 2316 72-73 -1.472272560161933 2316 74-75 -1.2859032893766837 2316 76-77 -1.1706010093433399 2316 78-79 -1.2116283626387983 2316 80-81 -1.1210030500423258 2316 82-83 -1.135270592059591 2316 84-85 -1.2041522353008922 2316 86-87 -1.057985050495489 2316 88-89 -1.1467421415796828 2316 90-91 -1.0998046372013448 2316 92-93 -1.2035516375474522 2316 94-95 -1.518387285552322 2316 96-97 -1.3034759608791902 2316 98-99 -1.1003052453164273 2316 100-101 -1.046659348127175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114523.0 3 24113.0 4 19339.0 5 17133.0 6 17718.0 7 18590.0 8 20734.0 9 24428.0 10 29448.0 11 34411.0 12 41170.0 13 48081.0 14 53935.0 15 63469.0 16 75590.0 17 93626.0 18 77588.0 19 52457.0 20 49622.0 21 51401.0 22 55120.0 23 60907.0 24 70320.0 25 84246.0 26 102039.0 27 124239.0 28 153290.0 29 190023.0 30 240128.0 31 310716.0 32 411601.0 33 565174.0 34 813000.0 35 1233930.0 36 1937704.0 37 2851940.0 38 2813629.0 39 608297.0 40 315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42608290709586 20.349223873568313 14.468973057994146 22.755720161341678 2 15.69668994197626 20.518366658506363 40.01426798760283 23.770675411914542 3 18.41807496094001 25.480960211641086 30.378332936880128 25.72263189053878 4 12.85665314762008 16.876825764851226 38.30111566074611 31.96540542678259 5 13.231155229431119 37.51468792105804 35.20119371499376 14.052963134517082 6 30.689904257642564 37.57885315766794 17.651456794933093 14.079785789756393 7 26.963101111767507 32.77487097182839 22.426513906814336 17.83551400958977 8 25.29318178816598 35.47993791396745 20.53543411194684 18.691446185919734 9 25.149708375065536 16.745121002617786 21.675894114537623 36.42927650777905 10-11 23.947807708914816 26.94966279142179 28.70882583061094 20.393703669052453 12-13 24.62750633984272 24.511781013800785 28.949995576553622 21.910717069802867 14-15 21.91666607334142 26.38070135913783 26.65589114236482 25.046741425155933 16-17 21.167050081002873 29.6903326979863 27.28213429381336 21.860482927197463 18-19 21.05438661322066 28.250189259350627 29.89742532394697 20.797998803481747 20-21 21.95802829590472 27.480432801865177 29.745512895782273 20.816026006447828 22-23 21.742758953639736 27.276068488093742 29.78053225356217 21.200640304704354 24-25 21.518540574493183 27.466647935348025 29.667682402917308 21.347129087241484 26-27 21.289075440284623 27.55251535246896 29.686079508355046 21.47232969889137 28-29 21.327812453991495 27.54855906753487 29.79324480492836 21.330383673545274 30-31 21.50163303261845 27.779818597182633 29.501961941058546 21.21658642914037 32-33 21.310428280230038 27.675895591476834 29.720556601098018 21.293119527195106 34-35 21.5472213502835 27.479272646570585 29.64524958501394 21.328256418131975 36-37 21.724674540116766 27.645418218227906 29.28319622931423 21.346711012341096 38-39 21.524414395700145 27.878033508867706 29.326550858911393 21.271001236520757 40-41 21.51594551037888 27.637716457742272 29.45054117248468 21.395796859394167 42-43 21.512416633975555 27.687628106796165 29.237828706148385 21.562126553079903 44-45 21.59633946241499 27.88701409033082 29.046216544816485 21.470429902437708 46-47 21.80382820063974 27.9251070183826 28.76825610415971 21.50280867681795 48-49 21.79453527874672 27.903421610731073 28.596523181232765 21.705519929289444 50-51 21.721855037582447 28.048381923017825 28.454134733100904 21.775628306298824 52-53 21.73070644617548 28.151125839088177 28.391327817573263 21.726839897163078 54-55 21.706840286090138 28.419876496192337 28.34104443552382 21.53223878219371 56-57 21.69482206239485 28.144839821993777 28.41137768299433 21.748960432617043 58-59 21.644318235772868 27.878465933785556 28.637399012966796 21.83981681747478 60-61 21.81641237359234 28.117047327592193 28.41524378022972 21.651296518585745 62-63 21.581485649257726 28.133533632527858 28.48537051514403 21.799610203070387 64-65 21.665205626022807 27.995652550996148 28.524298041076694 21.814843781904354 66-67 21.543047345103655 28.050646417917243 28.442318267058287 21.96398796992082 68-69 21.820647491465177 28.228056783004924 28.24697776189204 21.704317963637855 70-71 21.836771042117068 27.995171502271926 28.28258752703506 21.88546992857595 72-73 21.67679708964501 28.09726829218986 28.36535526300888 21.860579355156247 74-75 21.675767388407593 28.23797068839512 28.24510523025 21.841156692947283 76-77 21.789951656031782 28.22190191309891 28.082606796489014 21.905539634380293 78-79 21.741925401655667 28.238536617002957 28.245129210027326 21.774408771314054 80-81 21.722476518083326 28.195396818990105 28.224915930853335 21.857210732073238 82-83 21.65125325571396 28.171060046518793 28.37265475511519 21.80503194265206 84-85 21.650398674728002 28.137094212833098 28.184076983714224 22.028430128724676 86-87 21.70008106579197 28.24550000741523 28.148508074372753 21.905910852420046 88-89 21.550982032919904 28.115967916552904 28.271402709547104 22.061647340980088 90-91 21.52650879657137 28.062487009940433 28.238827183825915 22.17217700966228 92-93 21.62823855604447 28.157084908528034 28.189728697612786 22.024947837814707 94-95 21.487986656459952 27.92775205256642 28.342686597424954 22.241574693548674 96-97 21.526936938259666 28.038880607867238 28.21178848745096 22.222393966422132 98-99 21.475559768111207 28.016087348649865 28.24805213878753 22.260300744451403 100-101 21.50635509685929 27.944329846625358 28.1513179683834 22.39799708813195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3439.0 1 2345.0 2 1727.5 3 2337.5 4 2057.0 5 1998.5 6 3455.0 7 6618.0 8 10476.0 9 12692.0 10 11765.5 11 10186.0 12 10299.0 13 11229.5 14 12113.0 15 12784.5 16 13756.5 17 14852.0 18 16295.0 19 18194.5 20 20654.5 21 24812.0 22 31465.5 23 41815.0 24 54092.0 25 67898.0 26 88143.0 27 113766.0 28 147293.5 29 185287.5 30 217722.0 31 252348.5 32 290244.0 33 325905.0 34 357121.0 35 384758.5 36 411378.5 37 435912.5 38 458337.5 39 473774.0 40 484021.5 41 490096.5 42 490383.5 43 487525.5 44 483487.0 45 479698.5 46 475352.0 47 460918.0 48 441876.0 49 433237.0 50 424703.5 51 408313.5 52 385420.5 53 364132.5 54 346229.5 55 327024.0 56 298299.5 57 263088.0 58 235121.0 59 211831.5 60 187336.5 61 160101.0 62 135227.0 63 112239.5 64 91694.0 65 74808.5 66 58943.5 67 45050.0 68 34675.0 69 26913.5 70 20012.0 71 14625.5 72 11055.0 73 8287.0 74 6097.5 75 4412.0 76 2988.5 77 2125.5 78 1543.5 79 1095.5 80 818.0 81 627.0 82 482.0 83 403.5 84 338.5 85 288.5 86 245.0 87 210.0 88 184.0 89 148.0 90 122.0 91 110.5 92 92.5 93 75.5 94 67.0 95 50.0 96 32.0 97 27.5 98 24.5 99 16.0 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013907339784863413 2 0.004249669875905212 3 0.0035561473614345706 4 0.005680982299387177 5 0.0025084856906384934 6 0.007038515731968009 7 0.00126162074440936 8 0.004006199205931476 9 0.00967980360622854 10-11 0.007068027328328461 12-13 0.008949391596307333 14-15 0.0032352087510146453 16-17 0.00568836019847729 18-19 0.0037073942927818918 20-21 9.185484367190954E-4 22-23 9.923274276202277E-4 24-25 0.006898335649255858 26-27 0.0010919290653367561 28-29 8.447694458179633E-4 30-31 5.164529363079252E-4 32-33 0.0011657080562378884 34-35 0.0016637162448205306 36-37 0.0012763765425895864 38-39 0.0017596289329920023 40-41 0.0027076889660715506 42-43 0.0026634215715308713 44-45 0.007466433879194575 46-47 0.013892583986683187 48-49 0.00526413100079578 50-51 0.005839607129824611 52-53 0.013704447559885301 54-55 0.008355470719553218 56-57 0.005607203308486045 58-59 0.007042204681513065 60-61 0.001309577088495096 62-63 0.0019920327543305684 64-65 0.0022023028783987953 66-67 0.0022207476261240783 68-69 0.0018850532175239269 70-71 0.0021838581306735123 72-73 0.0013538444830357753 74-75 0.0015899372539193983 76-77 0.008218979586386123 78-79 0.006676998676552461 80-81 0.012962968701328922 82-83 0.01513207103382221 84-85 0.008510406600445596 86-87 0.00593920876754114 88-89 0.0029548485855903435 90-91 0.0026634215715308713 92-93 8.410804962729068E-4 94-95 0.0012505538957741902 96-97 0.0018075852770777384 98-99 0.0063413042679523095 100-101 0.011254985061967713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3553994E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.55996069189479 #Duplication Level Percentage of deduplicated Percentage of total 1 65.86342253586878 28.690080966961013 2 17.20639874216651 14.99020105715682 3 6.787424601330646 8.869798464994878 4 3.331051339234639 5.804018615989775 5 1.8688897468795131 4.0704381955778395 6 1.1085772631605286 2.8973749204320556 7 0.7250760332101728 2.210899845368909 8 0.5032573071703172 1.7537494814598271 9 0.3754463111727604 1.4718983900942106 >10 1.9917105888272144 15.759108227501773 >50 0.1506056846484188 4.519199991885697 >100 0.08351301404464306 6.541535921042456 >500 0.00355668840116377 1.0347245344817524 >1k 9.85216172719442E-4 0.6780758028563707 >5k 3.397108473099545E-5 0.1001366527488076 >10k+ 5.095662709649318E-5 0.6087589314477347 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35503 0.26193755139628955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29734 0.2193744515454264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17040 0.1257194004955292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.4755798180226434E-5 0.0 7.377899090113217E-6 0.0 3 0.0 1.4755798180226434E-5 0.0 7.377899090113217E-6 0.0 4 0.0 1.4755798180226434E-5 0.0 5.1645293630792516E-5 0.0 5 0.0 2.2133697270339652E-5 0.0 5.9023192720905736E-5 0.0 6 0.0 2.2133697270339652E-5 0.0 6.640109181101896E-5 0.0 7 0.0 2.2133697270339652E-5 0.0 1.0329058726158503E-4 0.0 8 0.0 2.2133697270339652E-5 0.0 1.0329058726158503E-4 0.0 9 0.0 2.2133697270339652E-5 0.0 1.6969167907260397E-4 0.0 10-11 0.0 2.2133697270339652E-5 0.0 1.7706957816271721E-4 0.0 12-13 0.0 2.2133697270339652E-5 0.0 1.9182537634294364E-4 0.0 14-15 0.0 3.320054590550948E-5 0.0 2.2502592224845312E-4 0.0 16-17 0.0 5.9023192720905736E-5 0.0 2.4715961951879276E-4 0.0 18-19 0.0 8.115688999124538E-5 0.0 2.6929331678913245E-4 0.0 20-21 7.377899090113217E-6 8.115688999124538E-5 0.0 2.8036016542430225E-4 0.0 22-23 7.377899090113217E-6 8.115688999124538E-5 0.0 3.39383358145208E-4 0.0 24-25 7.377899090113217E-6 9.222373862641521E-5 0.0 5.016971381276988E-4 0.0 26-27 7.377899090113217E-6 9.960163771652843E-5 0.0 7.746794044618877E-4 0.0 28-29 7.377899090113217E-6 1.1435743589675487E-4 0.0 0.0015530477584688321 0.0 30-31 7.377899090113217E-6 1.2173533498686809E-4 0.0 0.003644682150515929 0.0 32-33 7.377899090113217E-6 1.254242845319247E-4 0.0 0.008554673994986274 0.0 34-35 7.377899090113217E-6 1.291132340769813E-4 0.0 0.017474553994933154 0.0 36-37 7.377899090113217E-6 1.4386903225720773E-4 0.0 0.03267671507011144 0.0 38-39 7.377899090113217E-6 1.623137799824908E-4 0.0 0.05317620769199101 0.0 40-41 7.377899090113217E-6 1.8444747725283043E-4 0.0 0.07455735925513912 0.0 42-43 7.377899090113217E-6 1.8444747725283043E-4 0.0 0.09628158312597748 0.0 44-45 7.377899090113217E-6 1.8444747725283043E-4 0.0 0.12357612080985134 0.0 46-47 7.377899090113217E-6 1.8444747725283043E-4 0.0 0.157831706285247 0.0 48-49 7.377899090113217E-6 1.8813642679788703E-4 0.0 0.19409408031315345 0.0 50-51 7.377899090113217E-6 1.9920327543305685E-4 0.0 0.2315037176495725 0.0 52-53 7.377899090113217E-6 2.2133697270339652E-4 0.0 0.2685370821323958 0.0 54-55 7.377899090113217E-6 2.2133697270339652E-4 0.0 0.31172361445637353 0.0 56-57 7.377899090113217E-6 2.2133697270339652E-4 0.0 0.3647153746711117 0.0 58-59 7.377899090113217E-6 2.2133697270339652E-4 0.0 0.4239635933142659 0.0 60-61 7.377899090113217E-6 2.2871487179350973E-4 0.0 0.4872807233056175 0.0 62-63 7.377899090113217E-6 2.2871487179350973E-4 0.0 0.5519037414359191 0.0 64-65 7.377899090113217E-6 2.4347066997373616E-4 0.0 0.623635365339545 0.0 66-67 7.377899090113217E-6 2.4347066997373616E-4 0.0 0.7094255759593815 0.0 68-69 7.377899090113217E-6 2.4347066997373616E-4 0.0 0.8078983951151225 0.0 70-71 7.377899090113217E-6 2.4347066997373616E-4 0.0 0.9157263903171271 0.0 72-73 7.377899090113217E-6 2.508485690638494E-4 0.0 1.0298956897870841 0.0 74-75 7.377899090113217E-6 2.5453751860890603E-4 0.0 1.1489048910601554 0.0 76-77 7.377899090113217E-6 2.582264681539626E-4 0.0 1.2814894266590349 0.0 78-79 7.377899090113217E-6 2.656043672440758E-4 0.0 1.4382144480807648 0.0 80-81 7.377899090113217E-6 2.7298226633418903E-4 0.0 1.6116688556893266 0.0 82-83 7.377899090113217E-6 2.840491149693589E-4 0.0 1.7958913070199087 0.0 84-85 7.377899090113217E-6 3.1356071132981173E-4 0.0 1.9851713081767632 0.0 86-87 7.377899090113217E-6 3.209386104199249E-4 0.0 2.1940912767114993 0.0 88-89 7.377899090113217E-6 3.57828105870491E-4 0.0 2.4311689971236525 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20195 0.0 53.230614 1 GTATCAA 37745 0.0 40.977524 1 ATCAACG 44795 0.0 34.006706 3 TCAACGC 45120 0.0 33.762253 4 CAACGCA 47310 0.0 32.218155 5 TATCAAC 48000 0.0 31.853739 2 AACGCAG 49645 0.0 30.856348 6 GTGGTAT 9860 0.0 28.376522 1 ACGCAGA 56975 0.0 26.605097 7 CGCAGAG 57330 0.0 26.324348 8 TGGTATC 10265 0.0 25.542349 2 GTACATG 41165 0.0 24.187105 1 GCAGAGT 63190 0.0 23.16145 9 TACATGG 40985 0.0 22.76131 2 ACATGGG 42715 0.0 21.0284 3 GAGTACT 38905 0.0 20.836426 12-13 CAGAGTA 59935 0.0 20.246323 10-11 AGAGTAC 56675 0.0 20.166237 10-11 AGTACTT 41325 0.0 18.51272 12-13 GAGTACA 30310 0.0 18.33668 1 >>END_MODULE