FastQCFastQC Report
Wed 25 May 2016
SRR2049420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13553994
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347800.2566033303541377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292940.2161281759457766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199090.146886592985064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202150.054.420621
GTATCAA379650.040.292461
ATCAACG468850.032.32743
TCAACGC474250.031.9291424
CAACGCA492400.030.7906975
TATCAAC496450.030.7414472
AACGCAG517550.029.477016
GTGGTAT97850.026.4680461
ACGCAGA607550.024.9299587
CGCAGAG614550.024.5223398
TGGTATC103550.023.9447062
GTACATG397000.022.4318621
GCAGAGT671950.021.968219
TACATGG402350.020.8013252
AGAGTAC608450.020.2564410-11
GAGTACT424700.019.80003412-13
ACATGGG416300.019.567513
CAGAGTA642550.019.17034710-11
AGTACTT445450.018.87770712-13
GTACTTT466500.017.89372414-15
Produced by FastQC (version 0.11.5)