##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049420_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13553994 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25117467220363 33.0 31.0 34.0 31.0 34.0 2 32.4326330674191 34.0 31.0 34.0 31.0 34.0 3 32.51014475880689 34.0 31.0 34.0 31.0 34.0 4 36.11110887314839 37.0 35.0 37.0 35.0 37.0 5 36.01898163744207 37.0 35.0 37.0 35.0 37.0 6 36.05420830199571 37.0 35.0 37.0 35.0 37.0 7 35.98920244468162 37.0 35.0 37.0 35.0 37.0 8 35.980391610030225 37.0 35.0 37.0 35.0 37.0 9 37.63835176553863 39.0 37.0 39.0 35.0 39.0 10-11 37.6303602096917 39.0 37.0 39.0 35.0 39.0 12-13 37.61805217709259 39.0 37.0 39.0 35.0 39.0 14-15 38.91899018842712 40.0 38.0 41.0 36.0 41.0 16-17 38.90194609795459 40.0 38.0 41.0 36.0 41.0 18-19 38.91179677370375 40.0 38.0 41.0 35.0 41.0 20-21 38.895597157561085 40.0 38.0 41.0 35.0 41.0 22-23 38.82105145538651 40.0 38.0 41.0 35.0 41.0 24-25 38.73361637905403 40.0 38.0 41.0 35.0 41.0 26-27 38.582874096004474 40.0 38.0 41.0 34.0 41.0 28-29 38.41650247152241 40.0 38.0 41.0 34.0 41.0 30-31 38.260947142222435 40.0 38.0 41.0 34.0 41.0 32-33 38.04917875129648 40.0 37.5 41.0 34.0 41.0 34-35 37.89368687192868 40.0 37.0 41.0 33.0 41.0 36-37 37.72618012078211 40.0 37.0 41.0 33.0 41.0 38-39 37.90235689937593 40.0 37.5 41.0 33.0 41.0 40-41 37.835103291325055 40.0 37.5 41.0 33.0 41.0 42-43 37.67178408814405 40.0 37.0 41.0 33.0 41.0 44-45 37.478151974982424 40.0 37.0 41.0 33.0 41.0 46-47 37.20457040190515 40.0 37.0 41.0 32.0 41.0 48-49 36.979047172368524 40.0 36.5 41.0 31.5 41.0 50-51 36.77383201586189 39.5 36.0 41.0 31.0 41.0 52-53 36.60569692593932 39.0 36.0 41.0 31.0 41.0 54-55 36.50546248581783 39.0 36.0 41.0 31.0 41.0 56-57 36.42201339324778 39.0 35.5 41.0 31.0 41.0 58-59 36.18923591820979 39.0 35.0 40.5 30.0 41.0 60-61 35.953699920481 38.5 35.0 40.0 30.0 41.0 62-63 35.681932831016454 38.0 35.0 40.0 29.0 41.0 64-65 35.43309485012314 38.0 35.0 40.0 29.0 41.0 66-67 35.09786786831985 37.0 34.0 40.0 29.0 41.0 68-69 34.782294355449764 37.0 34.0 39.5 28.0 41.0 70-71 34.45127473127109 36.0 34.0 39.0 28.0 41.0 72-73 34.08118300775402 36.0 34.0 39.0 28.0 40.0 74-75 33.706734155260804 35.0 33.5 38.5 27.0 40.0 76-77 31.681856322202886 33.5 31.0 36.0 25.0 38.5 78-79 33.00037498172125 35.0 33.0 37.0 26.5 39.0 80-81 33.00329947025209 35.0 33.0 37.0 27.0 39.0 82-83 32.728406807617006 35.0 33.0 36.0 27.0 38.5 84-85 32.46705598364586 35.0 33.0 36.0 26.0 37.0 86-87 32.211879354528264 35.0 33.0 35.5 26.0 37.0 88-89 31.961549009096508 35.0 33.0 35.0 26.0 36.5 90-91 31.71356063017292 35.0 33.0 35.0 25.0 36.0 92-93 31.490622764035457 35.0 33.0 35.0 25.0 36.0 94-95 31.260726579929134 35.0 32.0 35.0 24.5 36.0 96-97 31.04858774468987 35.0 32.0 35.0 23.5 35.0 98-99 30.792595193711904 34.0 32.0 35.0 21.5 35.0 100-101 30.20666502434633 34.0 31.5 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39625035297321887 1101 2 0.39492632181558207 1101 3 0.3787181234802759 1101 4 0.27053251614496787 1101 5 0.30258725105008466 1101 6 0.3154862112529102 1101 7 0.30891175555959904 1101 8 0.317847188517419 1101 9 0.3805785900995602 1101 10-11 0.34693710901993313 1101 12-13 0.33213301464686396 1101 14-15 0.4296724114724455 1101 16-17 0.42204678785613226 1101 18-19 0.3567285777241338 1101 20-21 0.34194217563345575 1101 22-23 0.29817351448637197 1101 24-25 0.2857073221502162 1101 26-27 0.23462249177604377 1101 28-29 0.263633016896037 1101 30-31 0.20935825909261752 1101 32-33 0.1895861162314887 1101 34-35 0.16841578831576953 1101 36-37 0.10280025516352964 1101 38-39 0.2379704782930574 1101 40-41 0.3115783341328111 1101 42-43 0.3296097703028309 1101 44-45 0.31618217845961283 1101 46-47 0.30706395661738384 1101 48-49 0.2671025332917836 1101 50-51 0.26235155546460476 1101 52-53 0.180935830239207 1101 54-55 0.21422632668945596 1101 56-57 0.15054839408706755 1101 58-59 0.16306726790589465 1101 60-61 0.20806899476993124 1101 62-63 0.19275999843977587 1101 64-65 0.10158341655465364 1101 66-67 0.10917195627930454 1101 68-69 0.07143722128334673 1101 70-71 0.09773609895811575 1101 72-73 0.033331165913772054 1101 74-75 0.008454600399410594 1101 76-77 -0.039653308927114495 1101 78-79 0.01492160274388965 1101 80-81 0.04595294033750719 1101 82-83 0.06220319278855868 1101 84-85 0.051836441035213454 1101 86-87 0.010176607800985948 1101 88-89 -0.07495007829326994 1101 90-91 -0.11258442895460163 1101 92-93 -0.14970361674576793 1101 94-95 -0.185155844502912 1101 96-97 -0.24399358894217116 1101 98-99 -0.30288066271129566 1101 100-101 -0.3136047688404666 1102 1 0.333383135349024 1102 2 0.3278603128366413 1102 3 0.32102312546822986 1102 4 0.266502527038476 1102 5 0.2677866692183528 1102 6 0.2625271860120719 1102 7 0.278749268231671 1102 8 0.2650268785224341 1102 9 0.3643428407386011 1102 10-11 0.31560161945338905 1102 12-13 0.26701747685483923 1102 14-15 0.3522117016944861 1102 16-17 0.3708287709009852 1102 18-19 0.31604800585080284 1102 20-21 0.3232769581348691 1102 22-23 0.2969860329880589 1102 24-25 0.3033226860559495 1102 26-27 0.24503516895654087 1102 28-29 0.28345465616170173 1102 30-31 0.23346300561318145 1102 32-33 0.26682400939276363 1102 34-35 0.21717744162187813 1102 36-37 0.18228873427490555 1102 38-39 0.30152350207088574 1102 40-41 0.2852100975766305 1102 42-43 0.38240826589863985 1102 44-45 0.33197268250324896 1102 46-47 0.2729507443584467 1102 48-49 0.12144860695913451 1102 50-51 0.220992806452891 1102 52-53 0.24623915759365644 1102 54-55 0.2453734418807869 1102 56-57 0.19306984499128532 1102 58-59 0.21597491484404685 1102 60-61 0.28324730654455266 1102 62-63 0.17839638179895445 1102 64-65 0.20383835539281137 1102 66-67 0.1987904246417571 1102 68-69 0.16361042821232274 1102 70-71 0.1263133321639529 1102 72-73 0.11574337402151258 1102 74-75 0.08337316418803198 1102 76-77 0.08587829619641951 1102 78-79 0.18833447692695415 1102 80-81 0.16644620683815248 1102 82-83 0.21637702468552078 1102 84-85 0.2158063017464329 1102 86-87 0.17587350888205577 1102 88-89 0.12908746753504374 1102 90-91 0.09076923266767878 1102 92-93 0.07543252914413756 1102 94-95 0.017049329998350515 1102 96-97 -0.024486995092591002 1102 98-99 -0.03687298740472045 1102 100-101 -0.05760828937827611 1103 1 0.33764164611937275 1103 2 0.35489010867389936 1103 3 0.3211804905959639 1103 4 0.26142532794848705 1103 5 0.25492380955943617 1103 6 0.24895758047139793 1103 7 0.21707026525970718 1103 8 0.25916654995383936 1103 9 0.32252158013648824 1103 10-11 0.29763088368930823 1103 12-13 0.2787899470308872 1103 14-15 0.3865925030375763 1103 16-17 0.4222713587919458 1103 18-19 0.36303889737685324 1103 20-21 0.3099921350467767 1103 22-23 0.2548631289863508 1103 24-25 0.26639590954727055 1103 26-27 0.23970949216680992 1103 28-29 0.23156680901350768 1103 30-31 0.21759683221316806 1103 32-33 0.2637225748484724 1103 34-35 0.187457436879221 1103 36-37 0.18600917543164996 1103 38-39 0.27563319230038275 1103 40-41 0.2731744986567506 1103 42-43 0.348814590785544 1103 44-45 0.3554874635252361 1103 46-47 0.4089721453478319 1103 48-49 0.3378525772139511 1103 50-51 0.3825135369566226 1103 52-53 0.3145694339873799 1103 54-55 0.27568524415403317 1103 56-57 0.2604883666497244 1103 58-59 0.2385618372285805 1103 60-61 0.26561411814448377 1103 62-63 0.31515377509320075 1103 64-65 0.3198717633358896 1103 66-67 0.2888490399510957 1103 68-69 0.298086010454945 1103 70-71 0.2797257284390753 1103 72-73 0.23808962851438054 1103 74-75 0.1868353806928411 1103 76-77 0.14477702419410932 1103 78-79 0.28802112159849713 1103 80-81 0.2254139873441403 1103 82-83 0.27264543938354535 1103 84-85 0.24149143566821607 1103 86-87 0.25701948549493636 1103 88-89 0.22096631920936005 1103 90-91 0.2866083751140174 1103 92-93 0.22987711708073633 1103 94-95 0.1956504169143365 1103 96-97 0.1870456310346924 1103 98-99 0.17238753565759168 1103 100-101 0.09610314517158258 1104 1 0.33940331021519654 1104 2 0.30608060686274996 1104 3 0.30496245463632476 1104 4 0.22933286649960394 1104 5 0.22257904701936582 1104 6 0.2526109418893654 1104 7 0.26225792229084277 1104 8 0.26856944893179957 1104 9 0.32663469647141596 1104 10-11 0.2681534903472027 1104 12-13 0.2507508254325401 1104 14-15 0.3063896905461263 1104 16-17 0.3328804248649817 1104 18-19 0.29719486138307616 1104 20-21 0.2981397413998579 1104 22-23 0.2889405669264491 1104 24-25 0.28015547163074217 1104 26-27 0.28576070291020983 1104 28-29 0.28744673745080007 1104 30-31 0.22620564847242264 1104 32-33 0.2815109289196869 1104 34-35 0.2058678516757837 1104 36-37 0.23192117865917794 1104 38-39 0.334847779908479 1104 40-41 0.334970169400016 1104 42-43 0.3064313265754848 1104 44-45 0.31011530735857207 1104 46-47 0.326327519555079 1104 48-49 0.32820217562879606 1104 50-51 0.33400832297431293 1104 52-53 0.2733922393312298 1104 54-55 0.31662090247162666 1104 56-57 0.2552831332404679 1104 58-59 0.2573122236677605 1104 60-61 0.3088225698706779 1104 62-63 0.307953529289442 1104 64-65 0.2924562956354251 1104 66-67 0.2924233930160156 1104 68-69 0.2061883170599188 1104 70-71 0.20038400674518897 1104 72-73 0.2080910211856164 1104 74-75 0.22326513248576418 1104 76-77 0.13849404477719318 1104 78-79 0.292368905747459 1104 80-81 0.2566852279514009 1104 82-83 0.25700765149822047 1104 84-85 0.1925052569819954 1104 86-87 0.18758347941595588 1104 88-89 0.14861535738562281 1104 90-91 0.16622396533702855 1104 92-93 0.08599038297879957 1104 94-95 0.08262191511158434 1104 96-97 0.012982293213564589 1104 98-99 0.009015967989348894 1104 100-101 0.06357582077211887 1105 1 0.2782313129602514 1105 2 0.2639664908540311 1105 3 0.25131922331376444 1105 4 0.21589972204864694 1105 5 0.22756025909989575 1105 6 0.24184773984509178 1105 7 0.228377931984447 1105 8 0.2275799924573647 1105 9 0.2687709928452122 1105 10-11 0.22966512870986833 1105 12-13 0.2217334245343352 1105 14-15 0.2877195743941314 1105 16-17 0.28469593475443844 1105 18-19 0.29022383210173786 1105 20-21 0.2677914424389982 1105 22-23 0.1578842873095212 1105 24-25 0.19795583437398534 1105 26-27 0.2290682228785812 1105 28-29 0.21265610896229958 1105 30-31 0.23817145383922167 1105 32-33 0.2701532164081044 1105 34-35 0.24460586716180188 1105 36-37 0.18431938664535608 1105 38-39 0.28862555355446773 1105 40-41 0.3046567954539725 1105 42-43 0.3727544838325443 1105 44-45 0.3208960598858823 1105 46-47 0.3242294713116536 1105 48-49 0.31512197630279104 1105 50-51 0.31092961897151383 1105 52-53 0.3121973579061006 1105 54-55 0.3273575857701587 1105 56-57 0.31306738042269444 1105 58-59 0.31117846403862615 1105 60-61 0.4082200549334658 1105 62-63 0.39212153082225853 1105 64-65 0.4000669288068508 1105 66-67 0.38626358577037223 1105 68-69 0.3525400803175245 1105 70-71 0.2899425906935349 1105 72-73 0.3925577669725797 1105 74-75 0.3395160420488068 1105 76-77 0.3465827139248283 1105 78-79 0.42550828037566646 1105 80-81 0.381605753124461 1105 82-83 0.47243546625377064 1105 84-85 0.41447054657520965 1105 86-87 0.4438245945331474 1105 88-89 0.4296168426244691 1105 90-91 0.4342410501532079 1105 92-93 0.38012314593587604 1105 94-95 0.36941457129407596 1105 96-97 0.39297283859002974 1105 98-99 0.3784291132407489 1105 100-101 0.46594770434678523 1106 1 0.2740339896999018 1106 2 0.23975109628398883 1106 3 0.21179920644816974 1106 4 0.20093660828157311 1106 5 0.20029273716899354 1106 6 0.25011103533952195 1106 7 0.22902694049454908 1106 8 0.2514959234537528 1106 9 0.2948125770390817 1106 10-11 0.26927175818240556 1106 12-13 0.23182129512633054 1106 14-15 0.30700186675647956 1106 16-17 0.2897629158604431 1106 18-19 0.28363776289672415 1106 20-21 0.2677782597285443 1106 22-23 0.252595821143629 1106 24-25 0.3079275858565893 1106 26-27 0.25766350378390257 1106 28-29 0.33560298007767386 1106 30-31 0.3085280012658771 1106 32-33 0.2978327788963284 1106 34-35 0.25026567695612556 1106 36-37 0.3106834802228775 1106 38-39 0.3454690853637956 1106 40-41 0.3821411762060478 1106 42-43 0.3549800917301411 1106 44-45 0.36506183726941543 1106 46-47 0.44042136376101837 1106 48-49 0.44316690039263307 1106 50-51 0.4574707810599179 1106 52-53 0.4173420924236524 1106 54-55 0.39088312949033366 1106 56-57 0.3292521575720144 1106 58-59 0.41723191034326135 1106 60-61 0.43753768368702595 1106 62-63 0.4314659912429235 1106 64-65 0.383446384808785 1106 66-67 0.4184433814851971 1106 68-69 0.47131501297574374 1106 70-71 0.491655132157625 1106 72-73 0.47206751658956847 1106 74-75 0.4171932526069426 1106 76-77 0.3634872437783976 1106 78-79 0.37235389709727684 1106 80-81 0.42408524838214845 1106 82-83 0.44005745897867854 1106 84-85 0.4012299563913402 1106 86-87 0.4013675758614639 1106 88-89 0.429254505672219 1106 90-91 0.45693605775856483 1106 92-93 0.40771713076988547 1106 94-95 0.4404613649225304 1106 96-97 0.49547536723829566 1106 98-99 0.5385192141558832 1106 100-101 0.5867282330979293 1107 1 0.196384108231058 1107 2 0.1866407463471731 1107 3 0.168457356271432 1107 4 0.1387853862500421 1107 5 0.1582196950913115 1107 6 0.1535087457327009 1107 7 0.16383699231501936 1107 8 0.15626014550732492 1107 9 0.18304013553066767 1107 10-11 0.1575891847326858 1107 12-13 0.15023047277133372 1107 14-15 0.1265774400913955 1107 16-17 0.14204564554732713 1107 18-19 0.11172937051246379 1107 20-21 0.1368039996188415 1107 22-23 0.11967790863622696 1107 24-25 0.12294528881018607 1107 26-27 0.11988303680907109 1107 28-29 0.18629747224503745 1107 30-31 0.19398370101927043 1107 32-33 0.1980737904051324 1107 34-35 0.17779787999158003 1107 36-37 0.1854152796456816 1107 38-39 0.2127293796043972 1107 40-41 0.21656109545735802 1107 42-43 0.22926129566983633 1107 44-45 0.1799468397601771 1107 46-47 0.21386134159723724 1107 48-49 0.13157330454640004 1107 50-51 0.14047973129171965 1107 52-53 0.19197763213055907 1107 54-55 0.23531341776539705 1107 56-57 0.21406493106627522 1107 58-59 0.23668176599393576 1107 60-61 0.297430550356097 1107 62-63 0.29173666748679494 1107 64-65 0.2874675169707501 1107 66-67 0.27882622741420704 1107 68-69 0.3106498675956004 1107 70-71 0.34393722041490804 1107 72-73 0.33744525774521605 1107 74-75 0.2990988257194971 1107 76-77 0.29091865181344545 1107 78-79 0.3508835432686155 1107 80-81 0.3558471689360445 1107 82-83 0.38685503245304886 1107 84-85 0.3855361061566498 1107 86-87 0.3879489744838409 1107 88-89 0.36018430339198204 1107 90-91 0.4138556794368533 1107 92-93 0.40365606898708606 1107 94-95 0.49954956445935395 1107 96-97 0.49029396692556304 1107 98-99 0.535016160900728 1107 100-101 0.6621007125186047 1108 1 0.1415811144207879 1108 2 0.14028966260767106 1108 3 0.12329708927893535 1108 4 0.1198619840877484 1108 5 0.09079007680765727 1108 6 0.1093403607043868 1108 7 0.153321769220085 1108 8 0.10909508666971846 1108 9 0.20048732162219807 1108 10-11 0.13156733583949887 1108 12-13 0.11227301416545288 1108 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2316 96-97 -0.9750991948974033 2316 98-99 -0.9225502390590492 2316 100-101 -1.2414292500689506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 35.0 4 60.0 5 144.0 6 355.0 7 1017.0 8 2692.0 9 5942.0 10 10163.0 11 15266.0 12 22659.0 13 32049.0 14 41007.0 15 52061.0 16 65056.0 17 86593.0 18 73169.0 19 44403.0 20 36035.0 21 35678.0 22 36550.0 23 39761.0 24 47175.0 25 57522.0 26 73033.0 27 94276.0 28 121854.0 29 159226.0 30 208902.0 31 276035.0 32 372892.0 33 519776.0 34 766933.0 35 1193030.0 36 1960502.0 37 3110866.0 38 3277128.0 39 713905.0 40 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.166022399961264 21.799409228221876 15.625668471302365 23.408899900514495 2 14.999419716120551 21.515273566036637 39.25414965687257 24.23115706097024 3 17.403279352295424 25.981895914233753 31.048984440484794 25.565840292986024 4 12.695571966337225 17.787721405740992 37.90425264583153 31.612453982090255 5 13.078784683402692 37.600438908021516 35.502374750039415 13.818401658536377 6 30.719886968342653 38.12544521893428 17.34643428282631 13.808233529896762 7 26.84072311084098 32.80865403953993 22.485054958708112 17.865567890910974 8 25.296196825821234 35.93266309546839 20.32125733566062 18.449882743049763 9 24.9411298595948 16.749391415144697 21.655173736727612 36.65430498853289 10-11 23.944849605931594 27.15365701853742 28.725537096377213 20.175956279153773 12-13 24.613894222905383 24.554184881163803 29.089756059339855 21.74216483659096 14-15 21.806439832004383 26.481270185573553 26.7204960924603 24.991793889961762 16-17 21.093937329469085 29.76192478763086 27.427354623294065 21.716783259605986 18-19 20.984249281668824 28.22458118405768 30.137372883325458 20.653796650948042 20-21 21.90856336681021 27.42446048685647 29.973167198037647 20.693808948295672 22-23 21.636412115867838 27.209459440516206 30.053082508373546 21.10104593524241 24-25 21.46545771220132 27.382150198590217 29.91918091860614 21.233211170602324 26-27 21.247960595517913 27.46569093016914 29.943995326192564 21.34235314812038 28-29 21.320783458171345 27.469036569013117 30.050903627092335 21.159276345723207 30-31 21.492236038477948 27.65164133178189 29.785796513327846 21.070326116412314 32-33 21.27485193024873 27.55349119373084 29.986234855094107 21.185422020926325 34-35 21.465161142919207 27.416522149535393 29.905175585593675 21.213141121951725 36-37 21.640647719803514 27.56964591917997 29.548734426417717 21.240971934598804 38-39 21.475071586855208 27.728922720061817 29.638892960160778 21.157112732922197 40-41 21.427293445498332 27.56634099723006 29.713640303848614 21.29272525342299 42-43 21.423743849658933 27.62899009699958 29.52887124859952 21.41839480474197 44-45 21.501187408716202 27.789089828421137 29.345577252985194 21.364145509877464 46-47 21.679784962380776 27.85718435809009 29.025751429984304 21.437279249544826 48-49 21.652413769900786 27.836454096494133 28.866114848334067 21.645017285271013 50-51 21.661293330305895 27.95161802320123 28.678771629300474 21.708317017192403 52-53 21.645693893637418 28.06480447974315 28.594267840863065 21.695233785756365 54-55 21.661142238090235 28.31712414970447 28.54787708370523 21.473856528500065 56-57 21.7145534902979 28.00833217008269 28.5995057389819 21.67760860063751 58-59 21.6321250404312 27.757068672841328 28.79396672010275 21.816839566624722 60-61 21.78956951709847 28.01592657319697 28.576945455972826 21.617558453731732 62-63 21.578774643383568 28.02981384038736 28.601730398878882 21.78968111735019 64-65 21.59244760853049 27.939768416396742 28.676857742106904 21.79092623296587 66-67 21.493351405272833 27.996265099710477 28.614777721046774 21.895605773969915 68-69 21.76337602505132 28.11290948558915 28.396286277999376 21.72742821136016 70-71 21.780504306585826 27.888186659544363 28.44467103350682 21.886638000363 72-73 21.61073496714454 28.05678906576935 28.47514429782339 21.85733166926272 74-75 21.64841451653709 28.136199315264925 28.350275864730186 21.865110303467805 76-77 21.67689010674066 28.159935356580768 28.25007653792347 21.913097998755106 78-79 21.63413992433635 28.200554270622536 28.368176791699483 21.797129013341628 80-81 21.60215277164997 28.171384333160493 28.31796601946189 21.90849687572765 82-83 21.540236890680063 28.10418288039297 28.478054793304707 21.877525435622257 84-85 21.524024790897307 28.141262238658292 28.285172566045713 22.049540404398684 86-87 21.575276351958987 28.239324472702208 28.239073611723352 21.94632556361545 88-89 21.406018437342397 28.12146439392327 28.32839781831213 22.144119350422205 90-91 21.363004556240327 28.101617192515015 28.288501658405963 22.246876592838696 92-93 21.477837124414386 28.19413569449896 28.205977231711994 22.122049949374663 94-95 21.30702887222918 27.980096391273847 28.35777299621866 22.355101740278315 96-97 21.321675871485738 28.12918904605749 28.255178139569782 22.293956942886986 98-99 21.207863469125822 28.11156569068305 28.2798823601976 22.40068847999353 100-101 21.163866618493902 28.097723674492087 28.194750432584698 22.543659274429313 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3178.0 1 2292.0 2 1722.5 3 2133.5 4 1914.0 5 1834.5 6 3327.5 7 6973.5 8 10962.5 9 13055.5 10 12134.0 11 10355.0 12 10521.0 13 11527.0 14 12393.0 15 13249.0 16 14072.5 17 15026.5 18 16417.0 19 18205.5 20 20744.0 21 24562.5 22 31106.5 23 41606.0 24 53661.0 25 67587.5 26 88146.5 27 114393.0 28 148121.5 29 186184.5 30 219432.5 31 254423.5 32 292447.5 33 329344.5 34 361745.5 35 389665.0 36 415777.5 37 440531.0 38 463817.0 39 478280.5 40 488596.5 41 495678.0 42 495932.5 43 494809.0 44 491054.0 45 485824.5 46 479385.5 47 462303.0 48 444545.0 49 437070.5 50 426954.5 51 409677.5 52 384152.5 53 361521.5 54 344598.0 55 323722.0 56 293922.5 57 258823.5 58 229844.0 59 207874.0 60 183786.5 61 154356.0 62 129681.0 63 106902.5 64 85812.5 65 69056.0 66 53649.5 67 40541.0 68 31066.0 69 23741.0 70 16948.0 71 11928.0 72 8884.5 73 6457.0 74 4586.5 75 3157.5 76 2024.0 77 1351.0 78 884.0 79 534.0 80 327.0 81 223.0 82 157.5 83 104.0 84 72.0 85 51.0 86 37.5 87 28.0 88 20.0 89 17.5 90 12.0 91 9.5 92 7.5 93 3.0 94 1.0 95 1.5 96 0.5 97 0.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04642174107499236 2 0.0020879454425020406 3 2.7298226633418903E-4 4 2.3609277088362294E-4 5 0.002633909975170418 6 0.0019846548552404555 7 0.0 8 0.0 9 6.640109181101894E-5 10-11 1.4755798180226434E-5 12-13 7.377899090113217E-6 14-15 5.09075037217812E-4 16-17 0.0 18-19 6.640109181101894E-5 20-21 4.5005184449690625E-4 22-23 0.0 24-25 5.791650785738875E-4 26-27 0.007510701273735255 28-29 0.00760292501236167 30-31 0.0011288185607873221 32-33 0.0068024229610843855 34-35 0.008134133746849822 36-37 0.004673899073586722 38-39 0.0026080873283550222 40-41 0.001999410653420682 42-43 0.00127268759304453 44-45 8.115688999124538E-4 46-47 6.381882712947932E-4 48-49 0.00190349796524921 50-51 0.001375978180306115 52-53 0.001995721703875625 54-55 7.636125558267179E-4 56-57 1.623137799824908E-4 58-59 7.82057303552001E-4 60-61 0.00587280767573012 62-63 0.0026265320760803052 64-65 0.0010439727212510203 66-67 0.005562935913945365 68-69 0.007481189677374802 70-71 0.0024642182960978145 72-73 6.049877253892838E-4 74-75 0.0031798745078387967 76-77 0.0018850532175239269 78-79 0.0024051951033769088 80-81 8.705920926333595E-4 82-83 1.5124693134732094E-4 84-85 4.980081885826421E-4 86-87 0.004946881339920912 88-89 0.002652354722895701 90-91 0.0012210422994137374 92-93 7.746794044618879E-5 94-95 5.164529363079252E-4 96-97 3.172496608748683E-4 98-99 5.754761290288309E-4 100-101 7.377899090113217E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3553994E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.2283666931816 #Duplication Level Percentage of deduplicated Percentage of total 1 63.79196238847609 26.93830379818217 2 18.33144928817774 15.482143251172648 3 7.33190167873063 9.288426979433723 4 3.4151806099961086 5.768699964894371 5 1.9339440650183548 4.083364957084867 6 1.1702915572524717 2.965170061055514 7 0.761949807737873 2.252312711806788 8 0.543429789454188 1.8358521936855932 9 0.37804623822885597 1.4367847657415416 >10 2.0898459296215126 16.171070102277753 >50 0.15951084818840625 4.6606798763940445 >100 0.08753007223516882 6.64970489849259 >500 0.0038521336856381775 1.0709527154534895 >1k 0.0010178518828900566 0.6702236016892517 >5k 3.5096525664471916E-5 0.10396713722243091 >10k+ 5.264478849670787E-5 0.6223429854135007 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34780 0.2566033303541377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29294 0.2161281759457766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19909 0.146886592985064 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.2133697270339652E-5 0.0 3 0.0 0.0 0.0 2.2133697270339652E-5 0.0 4 0.0 0.0 0.0 3.6889495450566084E-5 0.0 5 0.0 1.4755798180226434E-5 0.0 4.4267394540679303E-5 0.0 6 0.0 1.4755798180226434E-5 0.0 5.1645293630792516E-5 0.0 7 0.0 1.4755798180226434E-5 0.0 9.591268817147182E-5 0.0 8 0.0 1.4755798180226434E-5 0.0 9.591268817147182E-5 0.0 9 0.0 1.4755798180226434E-5 0.0 1.9920327543305685E-4 0.0 10-11 0.0 2.2133697270339652E-5 0.0 2.0658117452317006E-4 0.0 12-13 0.0 3.6889495450566084E-5 0.0 2.176480231583399E-4 0.0 14-15 0.0 9.960163771652843E-5 0.0 2.4347066997373618E-4 0.0 16-17 0.0 1.5862483043743415E-4 0.0 2.8036016542430225E-4 0.0 18-19 0.0 1.6231377998249076E-4 0.0 3.3200545905509474E-4 0.0 20-21 0.0 1.6231377998249076E-4 0.0 3.430723076902646E-4 0.0 22-23 0.0 1.6231377998249076E-4 0.0 4.316070967716232E-4 0.0 24-25 0.0 1.6231377998249076E-4 0.0 5.828540281189441E-4 0.0 26-27 0.0 1.6231377998249076E-4 0.0 8.4845839536302E-4 0.0 28-29 0.0 1.6231377998249076E-4 0.0 0.0016231377998249077 0.0 30-31 0.0 1.6231377998249076E-4 0.0 0.0037701064350478537 7.377899090113217E-6 32-33 0.0 1.6231377998249076E-4 0.0 0.00863583088497752 7.377899090113217E-6 34-35 3.6889495450566085E-6 1.6231377998249076E-4 0.0 0.017529888238109005 7.377899090113217E-6 36-37 7.377899090113217E-6 1.9551432588800025E-4 0.0 0.032709915616016945 7.377899090113217E-6 38-39 7.377899090113217E-6 2.4347066997373618E-4 0.0 0.05310980660017999 7.377899090113217E-6 40-41 7.377899090113217E-6 2.766712158792456E-4 0.0 0.07439873442470168 7.377899090113217E-6 42-43 7.377899090113217E-6 3.098717617847551E-4 0.0 0.09601966770827847 7.377899090113217E-6 44-45 7.377899090113217E-6 3.172496608748683E-4 0.0 0.1232478043003413 7.377899090113217E-6 46-47 7.377899090113217E-6 3.246275599649815E-4 0.0 0.15752921242255236 7.377899090113217E-6 48-49 7.377899090113217E-6 3.246275599649815E-4 0.0 0.1938506096431797 7.377899090113217E-6 50-51 7.377899090113217E-6 3.467612572353212E-4 0.0 0.2312602469795988 7.377899090113217E-6 52-53 1.4755798180226434E-5 3.467612572353212E-4 0.0 0.2682382772192462 7.377899090113217E-6 54-55 1.4755798180226434E-5 3.467612572353212E-4 0.0 0.3112256062677909 7.377899090113217E-6 56-57 1.4755798180226434E-5 3.5413915632543443E-4 0.0 0.36398865161073557 7.377899090113217E-6 58-59 1.4755798180226434E-5 3.57828105870491E-4 0.0 0.4230192222307314 7.377899090113217E-6 60-61 1.4755798180226434E-5 3.6151705541554764E-4 0.0 0.48603016940984334 7.377899090113217E-6 62-63 1.4755798180226434E-5 3.6151705541554764E-4 0.0 0.5505794085492439 7.377899090113217E-6 64-65 1.4755798180226434E-5 3.652060049606043E-4 0.0 0.6219015590533683 7.377899090113217E-6 66-67 1.4755798180226434E-5 3.984065508661137E-4 0.0 0.7071605609387166 7.377899090113217E-6 68-69 1.4755798180226434E-5 4.057844499562269E-4 0.0 0.8053640867776686 7.377899090113217E-6 70-71 1.4755798180226434E-5 4.1316234904634013E-4 0.0 0.9127235853874511 7.377899090113217E-6 72-73 1.4755798180226434E-5 4.168512985913967E-4 0.0 1.0263284755770143 1.4755798180226434E-5 74-75 1.4755798180226434E-5 4.2422919768151E-4 0.0 1.144780645468782 1.4755798180226434E-5 76-77 1.4755798180226434E-5 4.2791814722656656E-4 0.0 1.2768339723331734 1.4755798180226434E-5 78-79 1.4755798180226434E-5 4.352960463166798E-4 0.0 1.4333302788831102 1.4755798180226434E-5 80-81 1.4755798180226434E-5 4.4267394540679303E-4 0.0 1.6064268583858015 1.4755798180226434E-5 82-83 1.4755798180226434E-5 4.4267394540679303E-4 0.0 1.7903578827023239 1.4755798180226434E-5 84-85 1.4755798180226434E-5 4.5742974358701946E-4 0.0 1.979671084405084 1.4755798180226434E-5 86-87 1.4755798180226434E-5 4.5742974358701946E-4 0.0 2.188148378994413 1.4755798180226434E-5 88-89 1.4755798180226434E-5 4.6480764267713267E-4 0.0 2.4249199165943263 1.4755798180226434E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20215 0.0 54.42062 1 GTATCAA 37965 0.0 40.29246 1 ATCAACG 46885 0.0 32.3274 3 TCAACGC 47425 0.0 31.929142 4 CAACGCA 49240 0.0 30.790697 5 TATCAAC 49645 0.0 30.741447 2 AACGCAG 51755 0.0 29.47701 6 GTGGTAT 9785 0.0 26.468046 1 ACGCAGA 60755 0.0 24.929958 7 CGCAGAG 61455 0.0 24.522339 8 TGGTATC 10355 0.0 23.944706 2 GTACATG 39700 0.0 22.431862 1 GCAGAGT 67195 0.0 21.96821 9 TACATGG 40235 0.0 20.801325 2 AGAGTAC 60845 0.0 20.25644 10-11 GAGTACT 42470 0.0 19.800034 12-13 ACATGGG 41630 0.0 19.56751 3 CAGAGTA 64255 0.0 19.170347 10-11 AGTACTT 44545 0.0 18.877707 12-13 GTACTTT 46650 0.0 17.893724 14-15 >>END_MODULE