FastQCFastQC Report
Wed 25 May 2016
SRR2049419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10596136
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355700.33568840566032754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294540.2779692521877786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169240.15971859930827614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182700.053.8976671
GTATCAA342200.041.393951
TATAACG12950.034.1111032
ATCAACG410300.033.9097823
TCAACGC416850.033.35594
TACCTGG100400.033.0693782
GTACCTG103750.032.690331
TATCAAC431800.032.329542
CAACGCA432200.032.169565
ATAACGC13050.032.0316543
AACGCAG466700.030.0866496
GTGGTAT82350.029.418181
TGGTATC82350.028.5511882
CGCAGAG517700.026.7852618
ACGCAGA525750.026.4745337
GTACATG292050.023.8118481
GCAGAGT568800.023.677379
ACCTGGG135850.023.4273053
GTACCGG8600.023.1985071
TACATGG288100.022.751942
Produced by FastQC (version 0.11.5)