##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049419_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10596136 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.476280882012084 31.0 31.0 34.0 30.0 34.0 2 31.508166939344683 33.0 31.0 34.0 30.0 34.0 3 31.538170706755746 33.0 31.0 34.0 28.0 34.0 4 35.150626133903906 37.0 35.0 37.0 32.0 37.0 5 35.174815800778696 37.0 35.0 37.0 33.0 37.0 6 35.265181005604305 37.0 35.0 37.0 33.0 37.0 7 35.210579875531984 37.0 35.0 37.0 33.0 37.0 8 35.18510521193763 37.0 35.0 37.0 32.0 37.0 9 36.774792150648125 39.0 37.0 39.0 33.0 39.0 10-11 36.713864988142845 39.0 37.0 39.0 32.5 39.0 12-13 36.67875874752834 39.0 37.0 39.0 33.0 39.0 14-15 37.86557835799767 40.0 38.0 41.0 33.0 41.0 16-17 37.85399555083098 40.0 38.0 41.0 33.0 41.0 18-19 37.875030294061915 40.0 38.0 41.0 33.0 41.0 20-21 37.83782795917304 40.0 38.0 41.0 33.0 41.0 22-23 37.723079101664986 40.0 38.0 41.0 32.5 41.0 24-25 37.61613455131191 40.0 38.0 41.0 32.0 41.0 26-27 37.472529939215576 40.0 37.0 41.0 32.0 41.0 28-29 37.31260650108681 40.0 37.0 41.0 32.0 41.0 30-31 37.13143630848076 40.0 37.0 41.0 31.5 41.0 32-33 36.87978759427021 40.0 36.0 41.0 31.0 41.0 34-35 36.681085727854 40.0 36.0 41.0 30.0 41.0 36-37 36.489044261040064 39.0 36.0 41.0 30.0 41.0 38-39 36.27839539809606 39.0 35.5 41.0 30.0 41.0 40-41 36.06975972184577 39.0 35.0 41.0 30.0 41.0 42-43 35.84947984812577 39.0 35.0 40.0 29.0 41.0 44-45 35.58925248788803 39.0 35.0 40.0 27.5 41.0 46-47 35.25888356850082 39.0 35.0 40.0 26.5 41.0 48-49 34.9795872759655 38.5 35.0 40.0 25.5 41.0 50-51 33.63738876133715 37.0 32.5 39.0 23.5 40.0 52-53 33.92248400737778 37.5 33.5 39.5 23.5 40.0 54-55 34.96267408232586 39.0 35.0 40.0 26.0 41.0 56-57 35.09166822698387 39.0 35.0 41.0 26.0 41.0 58-59 34.948084707482046 39.0 35.0 41.0 26.0 41.0 60-61 34.69768630753701 38.5 34.5 40.0 25.5 41.0 62-63 34.43051773778668 38.0 34.0 40.0 24.5 41.0 64-65 34.15467539299232 38.0 34.0 40.0 23.5 41.0 66-67 33.84890628999099 37.0 34.0 40.0 23.0 41.0 68-69 33.5059884565468 37.0 33.5 40.0 22.0 41.0 70-71 33.16814233037401 36.0 33.0 39.0 21.5 41.0 72-73 32.904729563682466 36.0 33.0 39.0 20.5 41.0 74-75 32.70171735243866 35.5 33.0 39.0 20.0 40.0 76-77 32.355200612751666 35.0 33.0 38.0 19.5 40.0 78-79 31.971409908291097 35.0 32.5 37.0 18.0 39.0 80-81 31.620163755920082 35.0 32.0 37.0 16.5 39.0 82-83 31.269522682608077 35.0 32.0 36.0 12.0 39.0 84-85 30.935935939289568 35.0 32.0 36.0 7.5 37.0 86-87 30.62641447788137 35.0 32.0 35.5 4.5 37.0 88-89 30.328667874780017 35.0 31.0 35.0 2.0 36.5 90-91 30.054131760860752 35.0 31.0 35.0 2.0 36.0 92-93 29.79256273230166 34.5 31.0 35.0 2.0 36.0 94-95 29.529033177754606 34.0 31.0 35.0 2.0 36.0 96-97 29.269784617713476 34.0 31.0 35.0 2.0 35.5 98-99 28.963713187524206 34.0 30.5 35.0 2.0 35.0 100-101 28.43406152959909 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2664618417812896 1101 2 0.30118102458707696 1101 3 0.2843685054095495 1101 4 0.24156388897097258 1101 5 0.24649375087543035 1101 6 0.30512038368070193 1101 7 0.26876968686837444 1101 8 0.2751437707461619 1101 9 0.30675038173006897 1101 10-11 0.3109804269646048 1101 12-13 0.3247029442996521 1101 14-15 0.42605676004539106 1101 16-17 0.3950712012684292 1101 18-19 0.4071629793590006 1101 20-21 0.34264329422082795 1101 22-23 0.35528105818275435 1101 24-25 0.32785738522640173 1101 26-27 0.33458781483405176 1101 28-29 0.3433007973107607 1101 30-31 0.2879043509153547 1101 32-33 0.27131380467258026 1101 34-35 0.2694712612829093 1101 36-37 0.2933358916200248 1101 38-39 0.2412996764581834 1101 40-41 0.21540684084279604 1101 42-43 0.11711107198111392 1101 44-45 0.051491875539490195 1101 46-47 0.03975815109486547 1101 48-49 -0.06369852835183565 1101 50-51 -0.08647344094072906 1101 52-53 0.029175144967766187 1101 54-55 0.28655998557127305 1101 56-57 0.26261802081791075 1101 58-59 0.2613441888025605 1101 60-61 0.19764896885119754 1101 62-63 0.17291707419600755 1101 64-65 0.1692924407347931 1101 66-67 0.24245757540335688 1101 68-69 0.18320085107368556 1101 70-71 0.14147759506073498 1101 72-73 0.11771263408390809 1101 74-75 0.06930254085003895 1101 76-77 0.05271018795713189 1101 78-79 0.01893474836744602 1101 80-81 -0.11883292691236136 1101 82-83 -0.1522064669235519 1101 84-85 -0.18018964134295246 1101 86-87 -0.21270770115369686 1101 88-89 -0.25794900613638916 1101 90-91 -0.2812941208510047 1101 92-93 -0.38229727192512897 1101 94-95 -0.486607538982085 1101 96-97 -0.46781686460178307 1101 98-99 -0.5819836647512311 1101 100-101 -0.6711712669352679 1102 1 0.2852909691351897 1102 2 0.2966937711985942 1102 3 0.3129021411118913 1102 4 0.34718218650443333 1102 5 0.32753413669336595 1102 6 0.3434890575549545 1102 7 0.29853718020503095 1102 8 0.28586812762099356 1102 9 0.3683502274785653 1102 10-11 0.3924589984097864 1102 12-13 0.3609756168208804 1102 14-15 0.43883446498087864 1102 16-17 0.4522398878380187 1102 18-19 0.3690594399135563 1102 20-21 0.38564952425941357 1102 22-23 0.40000521919444765 1102 24-25 0.3342414446953086 1102 26-27 0.39433030269027114 1102 28-29 0.3748966364835553 1102 30-31 0.28208380348863216 1102 32-33 0.29017780707813046 1102 34-35 0.2801499209935372 1102 36-37 0.21786537108393844 1102 38-39 0.1649807022979033 1102 40-41 0.1476867117935754 1102 42-43 0.12054852670779326 1102 44-45 0.1734707496813641 1102 46-47 0.13388232441493386 1102 48-49 0.05810351784532486 1102 50-51 -0.04664703094844924 1102 52-53 0.126950992213132 1102 54-55 0.22139562909747212 1102 56-57 0.20350769090969578 1102 58-59 0.2688177442987367 1102 60-61 0.3190324704560865 1102 62-63 0.3574345280698168 1102 64-65 0.2611418890552528 1102 66-67 0.25104812437576385 1102 68-69 0.24250586174795075 1102 70-71 0.2253071446480277 1102 72-73 0.23092688203073664 1102 74-75 0.22448715455963963 1102 76-77 0.18063766711941298 1102 78-79 0.11793131525134015 1102 80-81 0.0729865556380247 1102 82-83 0.019817520037303638 1102 84-85 0.010235063889570739 1102 86-87 -0.00516457339841736 1102 88-89 -0.002406306067090469 1102 90-91 -0.10284326478232586 1102 92-93 -0.22128561752127496 1102 94-95 -0.315184382029873 1102 96-97 -0.29101953938632974 1102 98-99 -0.3066624913227116 1102 100-101 -0.4421296827992194 1103 1 0.21452438851615696 1103 2 0.2053084687338469 1103 3 0.2210811564214623 1103 4 0.21775465829446006 1103 5 0.2589122562005812 1103 6 0.2373920288793201 1103 7 0.27160766903203637 1103 8 0.26689578682068316 1103 9 0.32036746179149844 1103 10-11 0.359673810457771 1103 12-13 0.3131105312235789 1103 14-15 0.3380963174005345 1103 16-17 0.3338539324901362 1103 18-19 0.3478296255443567 1103 20-21 0.39673819626079165 1103 22-23 0.32802321535546497 1103 24-25 0.39976533362269606 1103 26-27 0.3437052000537264 1103 28-29 0.3523061527938509 1103 30-31 0.31689962837699426 1103 32-33 0.24732992236314288 1103 34-35 0.2941946889988003 1103 36-37 0.27894734247455233 1103 38-39 0.2602308305039216 1103 40-41 0.1446707920788839 1103 42-43 0.13483782798619615 1103 44-45 0.1670605329915773 1103 46-47 0.08558641183609694 1103 48-49 0.0858061810864541 1103 50-51 0.02104187629721821 1103 52-53 0.12027380719040792 1103 54-55 0.29302506463987754 1103 56-57 0.4198769825176143 1103 58-59 0.3481460456177885 1103 60-61 0.3030158097629112 1103 62-63 0.29473250330029543 1103 64-65 0.26213741504356847 1103 66-67 0.2779168632195095 1103 68-69 0.18667613832835883 1103 70-71 0.17346013267135163 1103 72-73 0.1996631986185946 1103 74-75 0.139336387453028 1103 76-77 0.14049203185165027 1103 78-79 0.18067763068880538 1103 80-81 0.13774380665542552 1103 82-83 0.16186417825126398 1103 84-85 0.14452342982084687 1103 86-87 0.06290180454617911 1103 88-89 0.01225342370338467 1103 90-91 -0.023245364232884924 1103 92-93 -0.048283618945774265 1103 94-95 -0.07756916355823051 1103 96-97 -0.14911641695591982 1103 98-99 -0.2068001475411272 1103 100-101 -0.19013570786280454 1104 1 0.0962219022794848 1104 2 0.11866020567173763 1104 3 0.17444935790021532 1104 4 0.16763487852635706 1104 5 0.15132245520894827 1104 6 0.10735214040231966 1104 7 0.09033554757618134 1104 8 0.13398358732160176 1104 9 0.13067254698021458 1104 10-11 0.13849565308863276 1104 12-13 0.13647162863563267 1104 14-15 0.12060599784354764 1104 16-17 0.1806447995701177 1104 18-19 0.14220347072517114 1104 20-21 0.13330659253261246 1104 22-23 0.15416702084613831 1104 24-25 0.18343833561414158 1104 26-27 0.1376826572131975 1104 28-29 0.12382302212300544 1104 30-31 0.0650234209102507 1104 32-33 0.1108725147373022 1104 34-35 0.08964178610261087 1104 36-37 0.05307238081540078 1104 38-39 0.05764184212232237 1104 40-41 0.013709089986974732 1104 42-43 0.022899717485934445 1104 44-45 -0.02540454781965451 1104 46-47 0.015672548499019 1104 48-49 -0.03826659470423266 1104 50-51 -0.11652524278264309 1104 52-53 -0.07047522558198693 1104 54-55 0.10405088183796352 1104 56-57 0.057245878419173835 1104 58-59 0.15303633626383117 1104 60-61 0.07601334439859642 1104 62-63 0.05826741875560515 1104 64-65 0.04195264864065962 1104 66-67 0.056362851836901484 1104 68-69 0.09729842727055171 1104 70-71 0.051151589835711775 1104 72-73 0.022010312721299385 1104 74-75 0.04592076874995854 1104 76-77 0.11359265654051143 1104 78-79 0.16307082730531164 1104 80-81 0.13187300546627512 1104 82-83 0.07847143046616978 1104 84-85 0.1150825043531114 1104 86-87 0.07470710901709765 1104 88-89 0.04128285232011564 1104 90-91 0.05064626310707254 1104 92-93 0.001448289747425946 1104 94-95 -0.08545323088317502 1104 96-97 -0.0789371865682007 1104 98-99 -0.09879091610454438 1104 100-101 -0.13564323445171667 1105 1 0.26905612400131673 1105 2 0.283916177187546 1105 3 0.2565120860137746 1105 4 0.26458837605379415 1105 5 0.316382999716744 1105 6 0.2654463732936705 1105 7 0.3157708304361435 1105 8 0.2984483563758147 1105 9 0.37023825368846985 1105 10-11 0.3661982961456758 1105 12-13 0.32350736939836366 1105 14-15 0.43577771194863857 1105 16-17 0.3315075549914681 1105 18-19 0.3919533659756169 1105 20-21 0.4345756360694253 1105 22-23 0.3265577735882559 1105 24-25 0.41732156972879864 1105 26-27 0.4504897497329097 1105 28-29 0.370316101983704 1105 30-31 0.36712950013696855 1105 32-33 0.3779960292405491 1105 34-35 0.4264556002263191 1105 36-37 0.3876418926423497 1105 38-39 0.3721143027947491 1105 40-41 0.3826101199404306 1105 42-43 0.37505149995769926 1105 44-45 0.2741206289773501 1105 46-47 0.391058443371044 1105 48-49 0.3370371281407074 1105 50-51 0.2993260238138191 1105 52-53 0.37204606313310506 1105 54-55 0.5135397503540275 1105 56-57 0.4958015473678543 1105 58-59 0.5265857371935923 1105 60-61 0.5367047671874658 1105 62-63 0.5735404519873484 1105 64-65 0.5107278434940525 1105 66-67 0.4796875343132214 1105 68-69 0.49658197083579836 1105 70-71 0.49625988454235426 1105 72-73 0.4811191610436012 1105 74-75 0.4391392368169633 1105 76-77 0.4801803235251967 1105 78-79 0.5358069286190101 1105 80-81 0.534315990004032 1105 82-83 0.4959525176190418 1105 84-85 0.4896615422576964 1105 86-87 0.4228148861643035 1105 88-89 0.45996198954322054 1105 90-91 0.48245116659563436 1105 92-93 0.5059897918769494 1105 94-95 0.4572156396967877 1105 96-97 0.4376664231460907 1105 98-99 0.4494221926960833 1105 100-101 0.4789831264023867 1106 1 0.3193742560321411 1106 2 0.3323049212603202 1106 3 0.32426247934673214 1106 4 0.31408768511792573 1106 5 0.3291789712070283 1106 6 0.3507055593413142 1106 7 0.4236558103009571 1106 8 0.42407844139857076 1106 9 0.46191900936486974 1106 10-11 0.4456560704737953 1106 12-13 0.45347442887863565 1106 14-15 0.5429206063661312 1106 16-17 0.5177979847207794 1106 18-19 0.5382909120769952 1106 20-21 0.5654908220478205 1106 22-23 0.6008813197911493 1106 24-25 0.5892148804842847 1106 26-27 0.5679376962033018 1106 28-29 0.5507979851889928 1106 30-31 0.6200734752751913 1106 32-33 0.6683298443347141 1106 34-35 0.6397091709767082 1106 36-37 0.7048496615086464 1106 38-39 0.6730470738955532 1106 40-41 0.7208173462546128 1106 42-43 0.713433211051516 1106 44-45 0.6988646186412808 1106 46-47 0.7012566154385169 1106 48-49 0.6326427393368732 1106 50-51 0.6569560078705337 1106 52-53 0.7032797701141433 1106 54-55 0.7290679336167756 1106 56-57 0.7616588827989403 1106 58-59 0.7806877520579931 1106 60-61 0.7832193932604241 1106 62-63 0.7842933241890648 1106 64-65 0.79440570602695 1106 66-67 0.7322329602365372 1106 68-69 0.7586643719779076 1106 70-71 0.7927676253316349 1106 72-73 0.7985480737675417 1106 74-75 0.7855323466884556 1106 76-77 0.821700318067137 1106 78-79 0.8036244584012948 1106 80-81 0.770767292253197 1106 82-83 0.7719475770541528 1106 84-85 0.7816704314084539 1106 86-87 0.7998003318910634 1106 88-89 0.7714782893351959 1106 90-91 0.7299187999518608 1106 92-93 0.7323359876241824 1106 94-95 0.7801826911695819 1106 96-97 0.8122636520301185 1106 98-99 0.8189092921670387 1106 100-101 0.8680523392771597 1107 1 0.24000864733186233 1107 2 0.20916030627966364 1107 3 0.21855818495595614 1107 4 0.288066530013964 1107 5 0.231608697926184 1107 6 0.2751091612222538 1107 7 0.2915888743704329 1107 8 0.3324744930944661 1107 9 0.41827306164531564 1107 10-11 0.3342522403273378 1107 12-13 0.3426210096259368 1107 14-15 0.4768707768469298 1107 16-17 0.39851217930719685 1107 18-19 0.3699058170570808 1107 20-21 0.43075545451470276 1107 22-23 0.44951907316331585 1107 24-25 0.42218581667091115 1107 26-27 0.47596560865472526 1107 28-29 0.5074351411546871 1107 30-31 0.4613783543222496 1107 32-33 0.4492361112353862 1107 34-35 0.4305135444380568 1107 36-37 0.42583219944970097 1107 38-39 0.47040704512867393 1107 40-41 0.4449804598341771 1107 42-43 0.3987418892325678 1107 44-45 0.5005540616726663 1107 46-47 0.49682898630311456 1107 48-49 0.4547979049684159 1107 50-51 0.42117333564315373 1107 52-53 0.45362250442878604 1107 54-55 0.6066614764349083 1107 56-57 0.6094185618289174 1107 58-59 0.6162939044372138 1107 60-61 0.7031857733921285 1107 62-63 0.7036644083163424 1107 64-65 0.6619039988302191 1107 66-67 0.7349796379099942 1107 68-69 0.7200860067656407 1107 70-71 0.6836124129442425 1107 72-73 0.6863869930618165 1107 74-75 0.6763273475736113 1107 76-77 0.6784062011211418 1107 78-79 0.6416945443633253 1107 80-81 0.6685948802937887 1107 82-83 0.7241272455468071 1107 84-85 0.7404670770450323 1107 86-87 0.7892616375309096 1107 88-89 0.831783813572013 1107 90-91 0.817302306394172 1107 92-93 0.7749562388597404 1107 94-95 0.8063170843229308 1107 96-97 0.7983354832588141 1107 98-99 0.830933946965434 1107 100-101 0.8746336651858115 1108 1 0.14989690546250145 1108 2 0.1642381431679958 1108 3 0.17143585709563425 1108 4 0.17183638506946863 1108 5 0.2348438718278274 1108 6 0.22343143576271274 1108 7 0.23066026741723533 1108 8 0.23257026781521972 1108 9 0.2047202599962361 1108 10-11 0.22254499238535885 1108 12-13 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-1.0526406369763706 2316 52-53 -1.139040385432331 2316 54-55 -1.045153349263444 2316 56-57 -0.9310735663139766 2316 58-59 -0.8413748176577229 2316 60-61 -0.8419334293499006 2316 62-63 -1.0248278743641706 2316 64-65 -0.9694455125616628 2316 66-67 -0.7583605520541923 2316 68-69 -0.7872211168328391 2316 70-71 -0.9924245402765237 2316 72-73 -0.9865689211822968 2316 74-75 -0.8100956179641088 2316 76-77 -0.7172235611055768 2316 78-79 -0.7646480225922403 2316 80-81 -0.651024859622531 2316 82-83 -0.5800778945458269 2316 84-85 -0.6832710433639626 2316 86-87 -0.5684856060605625 2316 88-89 -0.6265550788225873 2316 90-91 -0.5889201231790189 2316 92-93 -0.6906911742157824 2316 94-95 -0.9924833447921166 2316 96-97 -0.7855731943792428 2316 98-99 -0.5435814703075827 2316 100-101 -0.4923264044029416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98148.0 3 19920.0 4 16125.0 5 14544.0 6 15140.0 7 16498.0 8 18145.0 9 21798.0 10 27217.0 11 33696.0 12 41566.0 13 49198.0 14 52916.0 15 60352.0 16 70708.0 17 87212.0 18 72473.0 19 46833.0 20 43716.0 21 44094.0 22 46529.0 23 51428.0 24 58467.0 25 68765.0 26 82841.0 27 100369.0 28 122472.0 29 150411.0 30 186853.0 31 236248.0 32 305587.0 33 410671.0 34 577448.0 35 870539.0 36 1369043.0 37 2154537.0 38 2362900.0 39 590391.0 40 338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.257747422890105 20.4635168567759 14.065890460570996 22.212845259762993 2 16.042076897986796 21.156903287085417 40.04619632513273 22.754823489795058 3 18.03557010653855 26.398740243089293 31.000401578188125 24.565288072184032 4 12.48369589633025 17.726869125095842 39.392007468836745 30.397427509737163 5 12.34902798627159 37.147604559450606 35.36105402894986 15.142313425327943 6 28.703127543857583 38.61561294432712 18.73810035038873 13.943159161426566 7 25.571973831970098 33.03430177266234 23.547215959104502 17.846508436263065 8 24.708360164213953 34.75049144987173 21.817760034293194 18.723388351621118 9 24.721415446624384 16.913553842628232 22.924594157161856 35.44043655358553 10-11 22.97256656006787 27.08976039503421 29.662921008966634 20.274752035931286 12-13 24.212159677227394 24.87409627700244 29.70922490460066 21.204519141169506 14-15 21.577536379810038 26.95833369904493 27.7241432226657 23.739986698479328 16-17 20.21833462091074 30.06196652072235 28.16566725460608 21.55403160376083 18-19 20.05573125888836 28.25232515982119 31.240149572426734 20.451794008863715 20-21 21.37853513530501 27.766252489259415 30.77634882445989 20.078863550975683 22-23 20.871852704338135 27.917983178875204 30.895275174743652 20.314888942043012 24-25 20.67069674597051 27.80074702758056 30.967705096351455 20.560851130097472 26-27 20.475308091162415 27.717651118064566 31.025560891473177 20.781479899299846 28-29 20.531396600783527 27.72952058262791 31.069965207349014 20.66911760923955 30-31 20.953374087745633 28.418220847226255 30.411690785589734 20.216714279438374 32-33 20.32837886587783 28.00893828492234 31.069939885291536 20.59274296390829 34-35 20.530369954976557 27.643755861038837 30.98972156700437 20.836152616980236 36-37 21.214235364988053 28.119974212854558 30.20213237533452 20.463658046822868 38-39 20.900505925268963 28.222475935931072 30.45118020523441 20.425837933565543 40-41 20.605705549119584 27.962494661812553 30.633593891933852 20.79820589713401 42-43 20.644500195006373 28.059569787389965 30.338834487130878 20.957095530472785 44-45 20.954625305808715 28.377562358739993 30.025751120759864 20.642061214691427 46-47 21.061776253102405 28.77861892916253 29.509722165196344 20.64988265253872 48-49 20.973348909080315 28.418902023093224 29.657556144535448 20.95019292329102 50-51 20.995625031558195 28.355651759873645 29.45315018019434 21.195573028373822 52-53 20.975103818644023 28.491581196859517 29.372731230975706 21.160583753520754 54-55 21.083807673384115 29.1127946332917 29.104361606671368 20.699036086652818 56-57 20.772046974073167 28.720239738448615 29.18463294061175 21.323080346866472 58-59 20.684653653033045 28.28965133079909 29.621686805435683 21.404008210732183 60-61 21.19576465511945 28.67777692525264 29.071827570719556 21.054630848908353 62-63 20.912902551929477 28.678625177539452 29.23721469406353 21.171257576467543 64-65 20.924804926298147 28.436762182839782 29.419484974660403 21.218947916201667 66-67 20.819695330762233 28.546445748130765 29.13134447005038 21.502514451056623 68-69 21.364355336771133 28.869300636471394 28.823495269877462 20.942848756880007 70-71 21.409301844043625 28.456448103176523 28.919315859843508 21.214934192936344 72-73 20.997405956668675 28.634231207766813 29.279979207260777 21.08838362830374 74-75 21.034563384608344 28.68369028123199 29.117614790721092 21.16413154343858 76-77 21.174437825077668 28.924981665144205 28.744726472643677 21.155854037134446 78-79 21.3078525288159 28.888453377123184 28.907598382060684 20.896095712000232 80-81 21.115547776054186 28.699521793484443 29.01334098488928 21.171589445572096 82-83 20.90436485697262 28.77404328871532 29.179619262239477 21.141972592072584 84-85 21.000985582464608 28.749697683137953 28.959211232006393 21.290105502391047 86-87 21.162793902197436 28.8954101127376 28.83732407253691 21.10447191252806 88-89 20.89178041099007 28.80634300173649 28.97852437523969 21.323352212033754 90-91 20.78337525150787 28.6130931648987 29.001920234461792 21.601611349131637 92-93 21.01951670669731 28.88964283860495 28.713279948660116 21.377560506037625 94-95 20.92487610658472 28.62771933907531 28.987071823214848 21.460332731125124 96-97 20.822943143512855 28.687206151983318 29.01439302298311 21.475457681520716 98-99 20.83550860117776 28.656879089997076 28.883155708940222 21.62445659988494 100-101 20.98102718031786 28.73402563826187 28.654534907492685 21.630412273927586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7177.0 1 4727.0 2 3994.0 3 5455.0 4 3986.0 5 3064.0 6 4733.0 7 8122.0 8 12270.5 9 14527.0 10 12990.5 11 10825.5 12 10813.0 13 11571.0 14 12301.5 15 13130.5 16 13902.5 17 15011.5 18 16515.5 19 18595.5 20 21503.5 21 25452.0 22 32023.0 23 43129.0 24 55387.0 25 68237.0 26 87775.0 27 114343.0 28 149882.0 29 185920.5 30 214114.5 31 244386.0 32 278191.5 33 309075.5 34 334245.5 35 354305.0 36 367445.5 37 376536.5 38 384772.5 39 384481.0 40 383450.5 41 378919.5 42 363840.0 43 349938.5 44 339050.0 45 328996.0 46 318633.0 47 308076.0 48 301610.0 49 300621.5 50 297417.0 51 284106.0 52 263924.5 53 247252.0 54 235834.5 55 226135.0 56 211551.0 57 192861.5 58 175129.5 59 157557.0 60 138567.0 61 117436.0 62 96461.0 63 77413.5 64 61169.5 65 48824.5 66 38892.5 67 30106.0 68 22723.0 69 16729.0 70 12454.5 71 9260.5 72 7066.0 73 5372.5 74 3967.5 75 2911.0 76 2121.5 77 1632.0 78 1334.5 79 1028.0 80 780.0 81 637.0 82 532.5 83 412.0 84 335.5 85 296.0 86 244.5 87 201.5 88 177.5 89 162.0 90 139.5 91 113.0 92 88.5 93 69.0 94 55.5 95 47.5 96 44.0 97 35.5 98 24.0 99 19.0 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01522253017515064 2 0.0046054524026494185 3 0.004161894486820479 4 0.006077687187102921 5 0.002812345934404768 6 0.007181863275443049 7 0.001443922577060166 8 0.004218518901607152 9 0.010834138029183468 10-11 0.0075546406727886465 12-13 0.00884284610918546 14-15 0.003350277874878163 16-17 0.0061956547179084906 18-19 0.00440254824966384 20-21 0.0010664264784823448 22-23 0.0010805825821790132 24-25 0.007653733398665325 26-27 0.0011749566068234684 28-29 7.21961288530083E-4 30-31 6.087124589567367E-4 32-33 0.0013542672536479335 34-35 0.0019960106212302295 36-37 0.0015005469918468394 38-39 0.0019205114015146654 40-41 0.0029350321664425596 42-43 0.0031001867095703565 44-45 0.008201102741603166 46-47 0.014448663173066107 48-49 0.0056954723872928775 50-51 0.00595500095506513 52-53 0.013769170195626027 54-55 0.008389850790892077 56-57 0.005558630051558417 58-59 0.006936490811367464 60-61 0.0015382966017046214 62-63 0.002373506719808051 64-65 0.0025386612629358474 66-67 0.0026849410011347535 68-69 0.0020384789323202347 70-71 0.0022366643840735904 72-73 0.001222143619145696 74-75 0.0015902023152590719 76-77 0.008300195467479843 78-79 0.006445745883216297 80-81 0.012655556704821455 82-83 0.014849752777805042 84-85 0.008290758065015398 86-87 0.006105999394496258 88-89 0.0031379363194281385 90-91 0.0027793150257792087 92-93 8.116166119423155E-4 94-95 0.0012551745277712555 96-97 0.0019205114015146654 98-99 0.006582588218950757 100-101 0.01123050893269018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0596136E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.460831474523175 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06782573089266 39.96941537596343 2 16.615745151883544 18.430460833844773 3 5.455579732369057 9.077129643982335 4 2.1911800974100575 4.860986804511539 5 1.0753165467734993 2.9818974891185634 6 0.6277545476514853 2.088947350479873 7 0.3953514828848175 1.5348565375834389 8 0.28274854649687553 1.2545175589543676 9 0.19730739275319228 0.9848548852346116 >10 0.9868305252568935 9.785956700430926 >50 0.06668252257154461 2.556543935056387 >100 0.03394258140902806 3.4173682379948356 >500 0.002389208470389565 0.9243722890991115 >1k 0.0012266739465419492 1.1049660157332748 >5k 6.814813157613662E-5 0.25340951544532964 >10k+ 5.111109868210246E-5 0.7743168265675033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35570 0.33568840566032754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29454 0.2779692521877786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16924 0.15971859930827614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 9.43740246444553E-6 0.0 7 0.0 0.0 0.0 1.887480492889106E-5 0.0 8 0.0 0.0 0.0 1.887480492889106E-5 0.0 9 0.0 0.0 0.0 2.831220739333659E-5 0.0 10-11 0.0 0.0 0.0 3.3030908625559355E-5 0.0 12-13 0.0 0.0 0.0 5.662441478667318E-5 0.0 14-15 0.0 0.0 0.0 6.606181725111871E-5 9.43740246444553E-6 16-17 0.0 0.0 0.0 8.965532341223254E-5 9.43740246444553E-6 18-19 0.0 0.0 0.0 9.43740246444553E-5 9.43740246444553E-6 20-21 0.0 4.718701232222765E-6 0.0 1.2740493327001467E-4 9.43740246444553E-6 22-23 0.0 9.43740246444553E-6 0.0 2.3593506161113823E-4 9.43740246444553E-6 24-25 0.0 9.43740246444553E-6 0.0 4.6715142199005375E-4 9.43740246444553E-6 26-27 0.0 9.43740246444553E-6 0.0 8.540849230323205E-4 9.43740246444553E-6 28-29 0.0 9.43740246444553E-6 0.0 0.0018166999744057646 9.43740246444553E-6 30-31 0.0 9.43740246444553E-6 0.0 0.004430860457057177 9.43740246444553E-6 32-33 0.0 9.43740246444553E-6 0.0 0.009918709990132252 1.4156103696668295E-5 34-35 0.0 2.3593506161113825E-5 0.0 0.019681702839601155 1.887480492889106E-5 36-37 0.0 3.774960985778212E-5 0.0 0.035333634826884064 1.887480492889106E-5 38-39 0.0 3.774960985778212E-5 0.0 0.05605817063880645 1.887480492889106E-5 40-41 0.0 4.718701232222765E-5 0.0 0.08009051601451699 1.887480492889106E-5 42-43 0.0 4.718701232222765E-5 0.0 0.10493447800216985 1.887480492889106E-5 44-45 0.0 4.718701232222765E-5 0.0 0.1355824425054567 1.887480492889106E-5 46-47 0.0 4.718701232222765E-5 0.0 0.17296399366712545 1.887480492889106E-5 48-49 0.0 4.718701232222765E-5 0.0 0.21244536687713333 1.887480492889106E-5 50-51 0.0 7.549921971556424E-5 0.0 0.2544984322587026 1.887480492889106E-5 52-53 0.0 8.0217920947787E-5 0.0 0.29553697687534397 1.887480492889106E-5 54-55 0.0 8.965532341223254E-5 0.0 0.3433515764614573 1.887480492889106E-5 56-57 0.0 1.1796753080556913E-4 0.0 0.40140575772149395 1.887480492889106E-5 58-59 0.0 1.4156103696668295E-4 0.0 0.4646127607271179 1.887480492889106E-5 60-61 0.0 1.4156103696668295E-4 0.0 0.5327508065204145 1.887480492889106E-5 62-63 0.0 1.5099843943112848E-4 0.0 0.603399201369254 1.887480492889106E-5 64-65 0.0 1.60435841895574E-4 0.0 0.6837917142626331 1.887480492889106E-5 66-67 0.0 1.651545431277968E-4 0.0 0.7784866105908795 1.887480492889106E-5 68-69 0.0 1.9346675052113335E-4 0.0 0.8849121981824318 1.887480492889106E-5 70-71 0.0 1.9818545175335613E-4 0.0 1.0017661154971964 1.887480492889106E-5 72-73 0.0 2.1234155545002441E-4 0.0 1.1310585292601 1.887480492889106E-5 74-75 0.0 2.2649765914669272E-4 0.0 1.2661219146300122 1.887480492889106E-5 76-77 0.0 2.453724640755838E-4 0.0 1.411042666874038 1.887480492889106E-5 78-79 0.0 2.453724640755838E-4 0.0 1.5794861447606938 1.887480492889106E-5 80-81 0.0 2.5480986654002934E-4 0.0 1.7670828309489421 1.887480492889106E-5 82-83 0.0 2.6424726900447484E-4 0.0 1.9653390632207817 1.887480492889106E-5 84-85 0.0 2.6424726900447484E-4 0.0 2.169545577746454 1.887480492889106E-5 86-87 0.0 2.689659702366976E-4 0.0 2.393933977442343 1.887480492889106E-5 88-89 0.0 2.7368467146892035E-4 0.0 2.646441117781048 2.831220739333659E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18270 0.0 53.897667 1 GTATCAA 34220 0.0 41.39395 1 TATAACG 1295 0.0 34.111103 2 ATCAACG 41030 0.0 33.909782 3 TCAACGC 41685 0.0 33.3559 4 TACCTGG 10040 0.0 33.069378 2 GTACCTG 10375 0.0 32.69033 1 TATCAAC 43180 0.0 32.32954 2 CAACGCA 43220 0.0 32.16956 5 ATAACGC 1305 0.0 32.031654 3 AACGCAG 46670 0.0 30.086649 6 GTGGTAT 8235 0.0 29.41818 1 TGGTATC 8235 0.0 28.551188 2 CGCAGAG 51770 0.0 26.785261 8 ACGCAGA 52575 0.0 26.474533 7 GTACATG 29205 0.0 23.811848 1 GCAGAGT 56880 0.0 23.67737 9 ACCTGGG 13585 0.0 23.427305 3 GTACCGG 860 0.0 23.198507 1 TACATGG 28810 0.0 22.75194 2 >>END_MODULE