FastQCFastQC Report
Wed 25 May 2016
SRR2049419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10596136
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353310.33343286647132503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302170.28516999026815054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205200.19365549857042227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189750.054.870651
GTATCAA345950.040.1096651
TACCTGG94550.032.40362
ATCAACG426500.032.0849083
GTACCTG98550.031.8731021
TCAACGC433550.031.6071434
CAACGCA448000.030.7255045
TATCAAC451400.030.505752
GTGGTAT84350.029.069941
AACGCAG484950.028.7070986
TGGTATC86500.026.5781232
TATAACG12000.025.729252
ATAACGC12300.025.1006433
CGCAGAG549250.025.0774888
ACGCAGA558250.024.7667837
GCAGAGT603900.022.4305869
ACCTGGG132350.022.3583973
GTACATG279900.021.476681
TACATGG271350.021.251222
AGAGTAC555900.020.44138710-11
Produced by FastQC (version 0.11.5)