##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10596136 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.054258741110914 33.0 31.0 34.0 30.0 34.0 2 32.23808763873925 34.0 31.0 34.0 30.0 34.0 3 32.31318916631496 34.0 31.0 34.0 30.0 34.0 4 35.95142927572844 37.0 35.0 37.0 35.0 37.0 5 35.83904576158705 37.0 35.0 37.0 35.0 37.0 6 35.86341964655795 37.0 35.0 37.0 35.0 37.0 7 35.79194868771031 37.0 35.0 37.0 35.0 37.0 8 35.783676143832054 37.0 35.0 37.0 33.0 37.0 9 37.394482384899554 39.0 37.0 39.0 34.0 39.0 10-11 37.38743670334168 39.0 37.0 39.0 34.0 39.0 12-13 37.37034929525253 39.0 37.0 39.0 34.0 39.0 14-15 38.60221249519636 40.0 38.0 41.0 34.0 41.0 16-17 38.58973506946306 40.0 38.0 41.0 34.0 41.0 18-19 38.620874109203584 40.0 38.0 41.0 34.0 41.0 20-21 38.60905956661938 40.0 38.0 41.0 34.0 41.0 22-23 38.51597233180095 40.0 38.0 41.0 34.0 41.0 24-25 38.41425383743659 40.0 38.0 41.0 34.0 41.0 26-27 38.232596722050374 40.0 38.0 41.0 34.0 41.0 28-29 38.04343087895437 40.0 38.0 41.0 33.0 41.0 30-31 37.860065688096114 40.0 37.0 41.0 33.0 41.0 32-33 37.60458548285904 40.0 37.0 41.0 33.0 41.0 34-35 37.42126290187291 40.0 36.5 41.0 32.5 41.0 36-37 37.22135432199058 39.0 36.0 41.0 31.5 41.0 38-39 37.360822331838705 40.0 37.0 41.0 32.5 41.0 40-41 37.27144810145887 40.0 37.0 41.0 32.0 41.0 42-43 37.08991423854884 40.0 36.5 41.0 31.5 41.0 44-45 36.86973610002741 40.0 36.0 41.0 31.0 41.0 46-47 36.5657446733413 40.0 36.0 41.0 30.0 41.0 48-49 36.30795414479391 39.0 36.0 41.0 30.0 41.0 50-51 36.091916760977774 39.0 35.0 41.0 29.5 41.0 52-53 35.91607742671479 39.0 35.0 40.5 29.0 41.0 54-55 35.81263570984744 39.0 35.0 40.0 28.5 41.0 56-57 35.730832682781724 39.0 35.0 40.0 28.5 41.0 58-59 35.51046975992003 39.0 35.0 40.0 28.0 41.0 60-61 35.26815322113646 38.0 35.0 40.0 28.0 41.0 62-63 34.984604529424686 38.0 34.0 40.0 27.0 41.0 64-65 34.73083839241021 38.0 34.0 40.0 26.5 41.0 66-67 34.40810140602197 37.0 34.0 40.0 26.0 41.0 68-69 34.088846632395054 37.0 34.0 39.5 26.0 41.0 70-71 33.75463985173464 36.0 33.5 39.0 26.0 41.0 72-73 33.38603515470167 36.0 33.0 39.0 25.0 40.0 74-75 33.017096939865624 35.0 33.0 38.5 24.5 40.0 76-77 30.992058001143057 33.5 30.0 35.5 22.0 38.5 78-79 32.31750786324373 35.0 32.5 37.0 24.5 39.0 80-81 32.32559260281295 35.0 33.0 37.0 24.5 39.0 82-83 32.04868666276084 35.0 33.0 36.0 24.0 38.5 84-85 31.770698158272033 35.0 32.5 36.0 23.5 37.0 86-87 31.4912206676094 35.0 32.0 35.5 23.0 37.0 88-89 31.221330209427286 35.0 32.0 35.0 21.5 36.5 90-91 30.95071434530474 35.0 32.0 35.0 20.0 36.0 92-93 30.69899645493414 34.5 32.0 35.0 18.5 36.0 94-95 30.439140314922348 34.0 31.5 35.0 17.5 36.0 96-97 30.198961866854106 34.0 31.0 35.0 9.0 35.0 98-99 29.91695435015179 34.0 31.0 35.0 2.0 35.0 100-101 29.30302456480362 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35770394231838054 1101 2 0.36360403976828337 1101 3 0.3574303964872101 1101 4 0.27719409148819096 1101 5 0.2818853948622362 1101 6 0.28873843369547814 1101 7 0.3421037075154416 1101 8 0.33217671573008545 1101 9 0.36359878210369345 1101 10-11 0.3399217113859194 1101 12-13 0.3191138893710459 1101 14-15 0.4349317012451763 1101 16-17 0.4204680290604941 1101 18-19 0.3932797119802629 1101 20-21 0.37538550007749905 1101 22-23 0.32008942668676355 1101 24-25 0.2951710085586825 1101 26-27 0.25796044369400306 1101 28-29 0.26090818826630624 1101 30-31 0.21194476034419552 1101 32-33 0.20502794228263355 1101 34-35 0.1939082355315449 1101 36-37 0.16173854724002723 1101 38-39 0.2529366312534762 1101 40-41 0.25703601727301617 1101 42-43 0.30123968531967193 1101 44-45 0.18257716022890946 1101 46-47 0.20191625772510946 1101 48-49 0.15698688658569893 1101 50-51 0.21959495102567672 1101 52-53 0.17390439597055973 1101 54-55 0.13128135025265664 1101 56-57 0.10323200721650494 1101 58-59 0.08699950021428293 1101 60-61 0.14130677776262246 1101 62-63 0.1250169915551993 1101 64-65 0.01611040345867565 1101 66-67 6.513536011354404E-4 1101 68-69 -0.06843741511089263 1101 70-71 -0.07573169151994819 1101 72-73 -0.0651381105123292 1101 74-75 -0.037087553560731124 1101 76-77 -0.08533339748672475 1101 78-79 -0.037131191943451824 1101 80-81 5.5863072235951E-4 1101 82-83 -0.03431422029206743 1101 84-85 -0.08782076928139304 1101 86-87 -0.1048538344066543 1101 88-89 -0.09683699726634387 1101 90-91 -0.11381283551535404 1101 92-93 -0.1620643350893083 1101 94-95 -0.24203280845169672 1101 96-97 -0.2991016012271466 1101 98-99 -0.39399357427965853 1101 100-101 -0.3833271207046849 1102 1 0.3590779939470963 1102 2 0.3540675453279576 1102 3 0.35866465475955067 1102 4 0.29005247124195677 1102 5 0.3135839732597603 1102 6 0.2957900733769563 1102 7 0.3151523574991657 1102 8 0.29982713962563423 1102 9 0.3531477873947111 1102 10-11 0.3396290013348704 1102 12-13 0.30452318499018105 1102 14-15 0.3282162271603255 1102 16-17 0.4294357152193484 1102 18-19 0.37799129647572016 1102 20-21 0.36662287707258656 1102 22-23 0.33816735774039586 1102 24-25 0.321433358885443 1102 26-27 0.2495927862677334 1102 28-29 0.28628194299292176 1102 30-31 0.23407118751701006 1102 32-33 0.2791045551785274 1102 34-35 0.2541445146416308 1102 36-37 0.22633595693342556 1102 38-39 0.37652973463530515 1102 40-41 0.35322095534306897 1102 42-43 0.37338881889694875 1102 44-45 0.3461087936205658 1102 46-47 0.29991618377319895 1102 48-49 0.11922690401989655 1102 50-51 0.18448275586406027 1102 52-53 0.24282999109831138 1102 54-55 0.29316531090077547 1102 56-57 0.20908651506756826 1102 58-59 0.24146433350792051 1102 60-61 0.25640230462341407 1102 62-63 0.2212255526113509 1102 64-65 0.25270356001787064 1102 66-67 0.24170319610318103 1102 68-69 0.13683389959921044 1102 70-71 0.11551832316197164 1102 72-73 -0.029173421145458178 1102 74-75 -0.016831148350512137 1102 76-77 0.056387374694015335 1102 78-79 0.15430830986765187 1102 80-81 0.13258683552218287 1102 82-83 0.19124509140242196 1102 84-85 0.12285727286037229 1102 86-87 0.058108922301915555 1102 88-89 0.04462175275903846 1102 90-91 0.04016525677652183 1102 92-93 -0.03876360463628714 1102 94-95 -0.0497738895016866 1102 96-97 -0.02321460964486377 1102 98-99 -0.06264426234224274 1102 100-101 -0.041726405083089446 1103 1 0.31863806265847217 1103 2 0.32962567620344885 1103 3 0.2814274776532599 1103 4 0.2820913194756116 1103 5 0.2800629190846635 1103 6 0.2927195029594145 1103 7 0.223036740332077 1103 8 0.25392116606876414 1103 9 0.3362053218322032 1103 10-11 0.31417276937012417 1103 12-13 0.28879933897773924 1103 14-15 0.33018521324393646 1103 16-17 0.3339109362729147 1103 18-19 0.31926103898954494 1103 20-21 0.28630678824866607 1103 22-23 0.23641118368998093 1103 24-25 0.2854803460971027 1103 26-27 0.23407320163812528 1103 28-29 0.2525787540769855 1103 30-31 0.24601379836300197 1103 32-33 0.1913344562879118 1103 34-35 0.20240606106408876 1103 36-37 0.2488347564170681 1103 38-39 0.3109848919882552 1103 40-41 0.38080213934767926 1103 42-43 0.37469572071107393 1103 44-45 0.3902926461925915 1103 46-47 0.42514977639151397 1103 48-49 0.37026428370809583 1103 50-51 0.3441430108327026 1103 52-53 0.28412630053612276 1103 54-55 0.19619783751309683 1103 56-57 0.20851724586947995 1103 58-59 0.165336362394072 1103 60-61 0.2609918701813143 1103 62-63 0.2521713600584974 1103 64-65 0.2475045374855327 1103 66-67 0.20055626369549628 1103 68-69 0.1580585409604538 1103 70-71 0.18400691108117684 1103 72-73 0.23518965507704337 1103 74-75 0.23475668237584557 1103 76-77 0.1826240962671868 1103 78-79 0.2880552483184573 1103 80-81 0.1814437974969465 1103 82-83 0.22503800653857553 1103 84-85 0.20513876357901495 1103 86-87 0.1658446402694871 1103 88-89 0.1616331319466049 1103 90-91 0.1542181240206233 1103 92-93 0.09298612750050239 1103 94-95 0.13492658385143486 1103 96-97 0.0837090862634291 1103 98-99 0.13227815417416977 1103 100-101 0.11912604363616452 1104 1 0.3100642976769592 1104 2 0.3094424965193312 1104 3 0.29727471490167545 1104 4 0.2773599807177973 1104 5 0.2163339922560823 1104 6 0.29017118591387003 1104 7 0.27318895449897695 1104 8 0.2662251202536581 1104 9 0.3458123207554493 1104 10-11 0.3037170508997775 1104 12-13 0.2644992073850787 1104 14-15 0.3627926927329739 1104 16-17 0.37248325337353094 1104 18-19 0.3127971433172476 1104 20-21 0.2985267980093198 1104 22-23 0.30544330972864486 1104 24-25 0.3223132227237713 1104 26-27 0.33624383294336724 1104 28-29 0.344816525754446 1104 30-31 0.31238087852158714 1104 32-33 0.3633648158185494 1104 34-35 0.3551641458338466 1104 36-37 0.4442064493771838 1104 38-39 0.4703822281907506 1104 40-41 0.48495482743015117 1104 42-43 0.47851362279583043 1104 44-45 0.474956927414226 1104 46-47 0.5788129325595364 1104 48-49 0.49963512520690756 1104 50-51 0.5003596758834803 1104 52-53 0.46520673236164356 1104 54-55 0.5047487234097048 1104 56-57 0.43877773492654626 1104 58-59 0.4721855507232391 1104 60-61 0.5146000512100599 1104 62-63 0.4876582791371291 1104 64-65 0.42330312080232346 1104 66-67 0.5636065648978885 1104 68-69 0.48542727002320163 1104 70-71 0.45756516413646153 1104 72-73 0.45221965117908525 1104 74-75 0.43995553149137123 1104 76-77 0.37744860458562 1104 78-79 0.505068793880092 1104 80-81 0.4832528202566806 1104 82-83 0.46358583450139434 1104 84-85 0.3714474851538512 1104 86-87 0.30937443855669144 1104 88-89 0.2833869065581034 1104 90-91 0.2770583343215307 1104 92-93 0.26306079778675695 1104 94-95 0.25599579430833685 1104 96-97 0.18831830600963073 1104 98-99 0.2374728848210701 1104 100-101 0.29601147098911795 1105 1 0.29544468464956 1105 2 0.30829864381771444 1105 3 0.26844521869305993 1105 4 0.22776921888816304 1105 5 0.23519738922959732 1105 6 0.24601428956150073 1105 7 0.23304074193271163 1105 8 0.23181097798438088 1105 9 0.2972260469652639 1105 10-11 0.2370898916093651 1105 12-13 0.21593587846628282 1105 14-15 0.25410639738817054 1105 16-17 0.24419435189825123 1105 18-19 0.29976728209302905 1105 20-21 0.2739649913640534 1105 22-23 0.21569777965521553 1105 24-25 0.25063180559426 1105 26-27 0.2923704696445526 1105 28-29 0.31549399738334216 1105 30-31 0.3295487851343708 1105 32-33 0.3808302145569016 1105 34-35 0.32926481142725805 1105 36-37 0.30488897678981175 1105 38-39 0.3761611483644103 1105 40-41 0.37459056120770384 1105 42-43 0.41833685460243686 1105 44-45 0.3813324707583874 1105 46-47 0.3909126900785864 1105 48-49 0.4206682027821529 1105 50-51 0.4118834041018147 1105 52-53 0.3926648260406864 1105 54-55 0.3623266853919773 1105 56-57 0.35740923045857187 1105 58-59 0.3600374055222204 1105 60-61 0.3749810174558874 1105 62-63 0.42060584852359995 1105 64-65 0.4227476401598267 1105 66-67 0.4792974999669539 1105 68-69 0.35195977889343055 1105 70-71 0.3399586826963201 1105 72-73 0.4287806173412747 1105 74-75 0.4409053700465151 1105 76-77 0.4521689173411687 1105 78-79 0.44977306670116945 1105 80-81 0.41131132357515554 1105 82-83 0.4659746442763151 1105 84-85 0.3959088090864995 1105 86-87 0.45128355132147036 1105 88-89 0.49985289225564955 1105 90-91 0.5117288148854726 1105 92-93 0.45234931801548584 1105 94-95 0.49379423728970906 1105 96-97 0.5205399810462765 1105 98-99 0.4740281226774421 1105 100-101 0.5755689582340864 1106 1 0.301292303766175 1106 2 0.2676036484309563 1106 3 0.2567337111760466 1106 4 0.2254729256914274 1106 5 0.24226923029095815 1106 6 0.25642378076872774 1106 7 0.24887258808833224 1106 8 0.24187374488121094 1106 9 0.3258203525177237 1106 10-11 0.2770994063776655 1106 12-13 0.2586682837160552 1106 14-15 0.3395595518887262 1106 16-17 0.31869049020613716 1106 18-19 0.3309490396284076 1106 20-21 0.3034309007725966 1106 22-23 0.2810192829359863 1106 24-25 0.3560076628418969 1106 26-27 0.31470607820141083 1106 28-29 0.4152780974096757 1106 30-31 0.4019915161810772 1106 32-33 0.37375451885930744 1106 34-35 0.4065637044204635 1106 36-37 0.4159874734807971 1106 38-39 0.42670224851759286 1106 40-41 0.42191117636975406 1106 42-43 0.4310760458537999 1106 44-45 0.4408711470163169 1106 46-47 0.4884585444848426 1106 48-49 0.47222649276238116 1106 50-51 0.54632798974162 1106 52-53 0.5623948921358135 1106 54-55 0.4743019166835154 1106 56-57 0.42532625897062815 1106 58-59 0.5143107837441008 1106 60-61 0.5202675371281629 1106 62-63 0.48627573812515124 1106 64-65 0.49507714400780145 1106 66-67 0.4893457429101815 1106 68-69 0.524324140230064 1106 70-71 0.48298012339340346 1106 72-73 0.4264956249381484 1106 74-75 0.4443242832915075 1106 76-77 0.37840774283183976 1106 78-79 0.45232406847201645 1106 80-81 0.5266928298163194 1106 82-83 0.46596484323877263 1106 84-85 0.43769125041768575 1106 86-87 0.42579214023596634 1106 88-89 0.4699512423125931 1106 90-91 0.481900688072308 1106 92-93 0.4394298666080161 1106 94-95 0.5405720109151382 1106 96-97 0.5007667890704255 1106 98-99 0.5621081350326271 1106 100-101 0.6826294844690253 1107 1 0.21408335481296348 1107 2 0.21009079144738507 1107 3 0.18646858542243905 1107 4 0.15113317368309964 1107 5 0.17611469881304487 1107 6 0.1490658221522949 1107 7 0.15800150212456288 1107 8 0.17601031378732301 1107 9 0.26597921473030084 1107 10-11 0.18740730265452754 1107 12-13 0.1793670552904203 1107 14-15 0.1942859780166799 1107 16-17 0.21325362716962815 1107 18-19 0.14133446878019384 1107 20-21 0.18085970217780556 1107 22-23 0.1843953641609346 1107 24-25 0.19926769193293126 1107 26-27 0.2101536637493311 1107 28-29 0.24341686996280743 1107 30-31 0.21102543046315958 1107 32-33 0.2737356504173576 1107 34-35 0.2682646817052401 1107 36-37 0.22595042607672156 1107 38-39 0.25116434188363 1107 40-41 0.2151972405373428 1107 42-43 0.2112553409160327 1107 44-45 0.15927380352307097 1107 46-47 0.23366099397128437 1107 48-49 0.1591524390593122 1107 50-51 0.28204125165095917 1107 52-53 0.25465010306171365 1107 54-55 0.27716408329727216 1107 56-57 0.3212669257601988 1107 58-59 0.3655306831403209 1107 60-61 0.44420348624216643 1107 62-63 0.37316460850728816 1107 64-65 0.3506814304335606 1107 66-67 0.381606866598581 1107 68-69 0.3975483989915318 1107 70-71 0.4684588161852119 1107 72-73 0.4278783945711453 1107 74-75 0.3821171243916126 1107 76-77 0.3793802792486467 1107 78-79 0.45803446055965935 1107 80-81 0.4824598268017226 1107 82-83 0.5011311223894737 1107 84-85 0.5236401670777582 1107 86-87 0.5005823144370289 1107 88-89 0.4826757837710538 1107 90-91 0.5681187099138612 1107 92-93 0.5972754367755044 1107 94-95 0.5999151786868104 1107 96-97 0.6356710461969257 1107 98-99 0.6806118136467738 1107 100-101 0.8527463271117242 1108 1 0.10808443132620482 1108 2 0.14797305160530527 1108 3 0.06863610743928916 1108 4 0.08777078343443634 1108 5 0.04051378206732892 1108 6 0.0922347953556013 1108 7 0.12291062946377451 1108 8 0.08927468844670727 1108 9 0.12947918150142357 1108 10-11 0.1104029009805032 1108 12-13 0.04822700350458575 1108 14-15 0.08476889894910755 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-1.1046194023359774 2316 54-55 -1.6907259202656775 2316 56-57 -1.2500832490507179 2316 58-59 -0.8173227133505065 2316 60-61 -0.7546543052046459 2316 62-63 -1.3150974677679486 2316 64-65 -1.5704305262116165 2316 66-67 -1.1855701254048725 2316 68-69 -1.170398479095077 2316 70-71 -0.9964136844323548 2316 72-73 -1.0674090473357865 2316 74-75 -0.8468143612026253 2316 76-77 -0.8936268275302055 2316 78-79 -0.9175291548867648 2316 80-81 -0.7329135551875439 2316 82-83 -0.8285515018464693 2316 84-85 -0.9275718838899927 2316 86-87 -1.1159360078308644 2316 88-89 -0.80760362881432 2316 90-91 -0.6994769541354202 2316 92-93 -0.7056928638922457 2316 94-95 -0.9734258815270493 2316 96-97 -1.0274565882107964 2316 98-99 -0.9724165810781855 2316 100-101 -1.2813791636720175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 30.0 4 71.0 5 198.0 6 468.0 7 1398.0 8 3759.0 9 7686.0 10 13205.0 11 19808.0 12 28882.0 13 39838.0 14 46660.0 15 56118.0 16 67601.0 17 86924.0 18 74308.0 19 45284.0 20 37376.0 21 36299.0 22 38065.0 23 42052.0 24 50372.0 25 61926.0 26 78082.0 27 99456.0 28 126401.0 29 160518.0 30 203486.0 31 258005.0 32 330819.0 33 436297.0 34 605979.0 35 901325.0 36 1408487.0 37 2247206.0 38 2434055.0 39 547482.0 40 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.287665779700795 22.189578545956973 15.296965183040696 23.22579049130154 2 14.997128140429874 22.305345396087287 38.99707830993848 23.700448153544354 3 16.651552639518798 26.925457693606063 31.65429373797968 24.76869592889546 4 12.104873094597542 18.769000467246457 38.64845446744143 30.477671970714564 5 12.101504889077475 37.39031569299115 35.47711635407929 15.031063063852084 6 28.802414661165436 39.217075449101344 18.269567073722023 13.710942816011192 7 25.423512872994458 33.14479919850029 23.40088877681449 18.03079915169077 8 24.73426162140614 35.0859218869973 21.525365472847838 18.654451018748723 9 24.37807193783461 16.704839440772712 22.81685911287725 36.10022950851543 10-11 22.859644578778585 27.342096500029278 29.565171968656674 20.233086952535462 12-13 24.08434188105654 24.956428690440962 29.694590070125095 21.2646393583774 14-15 21.376512793047432 27.075451198378648 27.675366025431558 23.872669983142362 16-17 20.079763038149 30.160097982887347 28.22921015736302 21.53092882160063 18-19 19.95267044716712 28.216344687417894 31.44615655832017 20.384828307094818 20-21 21.226375764215145 27.727249412483395 30.96805060096025 20.078324222341205 22-23 20.675621754949162 27.872532968621773 31.090946737565467 20.3608985388636 24-25 20.586904194027277 27.728787591874394 31.152032834102005 20.532275379996324 26-27 20.387674920710666 27.672907567568178 31.181906590915354 20.7575109208058 28-29 20.43804469495195 27.654822196089242 31.3067299143589 20.600403194599913 30-31 20.876047675063703 28.28946694757084 30.678424916370734 20.156060460994723 32-33 20.256699991529377 27.927436725743167 31.259973332874015 20.55588994985344 34-35 20.377199467028966 27.595639490916863 31.20268873363694 20.824472308417228 36-37 21.08926198575748 28.061841679754167 30.439492285032106 20.409404049456242 38-39 20.77074362453706 28.12764195216659 30.728238336149843 20.373376087146504 40-41 20.459945035551353 27.87414330394472 30.918219619967648 20.74769204053628 42-43 20.462347924967556 27.991158552899613 30.60838601121574 20.93810751091709 44-45 20.790807858078818 28.283264599390122 30.285958905159994 20.639968637371066 46-47 20.866278519706242 28.746084929109344 29.69500727746059 20.692629273723824 48-49 20.790042088383764 28.340483808078332 29.8834414182105 20.98603268532741 50-51 20.823382619038384 28.290884659672123 29.635985293658262 21.24974742763123 52-53 20.82049739606878 28.465806875148523 29.477245070783027 21.23645065799967 54-55 21.014416022865863 29.048513045937746 29.2030091948354 20.734061736360985 56-57 20.73296174712492 28.61267283531671 29.280139764557838 21.37422565300053 58-59 20.551559437611054 28.266572983935756 29.688645695482208 21.493221882970985 60-61 21.102834163002253 28.6151944909776 29.176069840648733 21.105901505371413 62-63 20.808345320359738 28.609961248436356 29.32409120738577 21.257602223818132 64-65 20.772849179494273 28.423604283341696 29.535422053513273 21.26812448365076 66-67 20.69023511665134 28.513222863676056 29.26182022281243 21.534721796860172 68-69 21.178633750520394 28.835905678040895 28.943811738892062 21.04164883254665 70-71 21.28482769796187 28.381093110023077 29.018942432224115 21.31513675979094 72-73 20.839020385998715 28.615133448278453 29.358220166785397 21.18762599893744 74-75 20.88622686335954 28.607701117082097 29.19552506520406 21.310546954354304 76-77 20.95011449214948 28.934902603576866 28.86958502812827 21.24539787614538 78-79 21.097257964012858 28.877564767585103 29.029562704516643 20.9956145638854 80-81 20.90013365845487 28.650106230748996 29.115346184835605 21.33441392596053 82-83 20.68648810538613 28.67344502862581 29.30695996775046 21.333106898237606 84-85 20.756682909943127 28.730969430052717 29.086266034413775 21.426081625590378 86-87 20.890590537024295 28.860471459406302 28.934672000951338 21.314266002618066 88-89 20.584220533979057 28.74610312408351 29.08360389974261 21.58607244219482 90-91 20.466378076510132 28.578853209214746 29.12914600614921 21.825622708125913 92-93 20.656045457370244 28.86187503158584 28.804646566332192 21.677432944711725 94-95 20.56100701688175 28.604522319722037 29.086209500798336 21.748261162597878 96-97 20.412037428297324 28.702058837588414 29.131815992118987 21.754087741995274 98-99 20.369169803245494 28.63913020602681 29.049754048021093 21.941945942706603 100-101 20.44198094673289 28.77734528781743 28.761929290164325 22.018744475285352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6663.0 1 4599.5 2 4203.0 3 5625.0 4 4078.0 5 2948.5 6 4891.5 7 9327.0 8 13620.0 9 15438.0 10 13455.0 11 10855.5 12 10820.5 13 11636.0 14 12518.5 15 13463.0 16 14468.5 17 15454.0 18 16840.5 19 18720.0 20 21305.5 21 25636.0 22 32257.0 23 42764.5 24 54795.0 25 68089.5 26 88143.0 27 114553.0 28 150515.5 29 187305.5 30 215199.0 31 244742.0 32 279115.0 33 310676.5 34 336212.5 35 356243.0 36 369167.5 37 379638.0 38 386721.5 39 386472.5 40 386021.5 41 380721.0 42 367246.5 43 353861.5 44 342806.5 45 332246.5 46 320445.5 47 309970.0 48 303470.0 49 301980.0 50 299452.5 51 286253.5 52 264936.5 53 246655.0 54 234281.0 55 223908.0 56 209264.5 57 189861.0 58 171951.0 59 155225.0 60 135789.5 61 114244.0 62 93550.5 63 74544.5 64 58252.5 65 46032.0 66 36292.0 67 27642.5 68 20350.0 69 14781.5 70 10913.5 71 7785.5 72 5640.5 73 4122.0 74 2997.5 75 2166.5 76 1442.5 77 986.0 78 704.0 79 506.0 80 338.5 81 192.5 82 126.0 83 94.5 84 58.0 85 42.5 86 33.5 87 23.5 88 19.0 89 16.0 90 13.5 91 12.5 92 7.0 93 2.5 94 3.0 95 3.5 96 1.5 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05048066578231914 2 0.0020196041273913435 3 1.7931064682446507E-4 4 1.4156103696668295E-4 5 0.002566973470329184 6 0.001868605687960215 7 0.0 8 0.0 9 1.4156103696668295E-4 10-11 4.2468311090004886E-5 12-13 4.718701232222765E-6 14-15 4.577140195256082E-4 16-17 0.0 18-19 7.549921971556424E-5 20-21 4.954636293833904E-4 22-23 0.0 24-25 7.502734959234197E-4 26-27 0.0072101754828363855 28-29 0.00713467626312082 30-31 0.0010805825821790132 32-33 0.006544838609092975 34-35 0.0076773269048264385 36-37 0.00440254824966384 38-39 0.0026235978851158574 40-41 0.001849730883031324 42-43 0.0012551745277712555 44-45 8.068979107100928E-4 46-47 5.992750564922911E-4 48-49 0.002071509840945794 50-51 0.0015382966017046214 52-53 0.002208352176680254 54-55 7.455547946911968E-4 56-57 2.8784077516558865E-4 58-59 8.352101181034293E-4 60-61 0.006082405888335144 62-63 0.0028831264528881096 64-65 0.0010381142710890084 66-67 0.005539755246629526 68-69 0.007634858593736434 70-71 0.0027840337270114315 72-73 6.087124589567367E-4 74-75 0.0034116209908970595 76-77 0.002010166724926898 78-79 0.0025339425617036246 80-81 9.295841427478847E-4 82-83 1.5571714066335123E-4 84-85 5.615254466345091E-4 86-87 0.004964073696298349 88-89 0.002911438660281446 90-91 0.001325955046254597 92-93 9.909272587667807E-5 94-95 4.482766170611627E-4 96-97 3.350277874878163E-4 98-99 5.992750564922911E-4 100-101 4.718701232222765E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0596136E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.38112449194878 #Duplication Level Percentage of deduplicated Percentage of total 1 70.84978040336215 38.52890728342471 2 17.381418782806907 18.904421973490386 3 5.613294121469871 9.157717392887323 4 2.3126648065633857 5.030612510154885 5 1.160954913147985 3.1567016830720087 6 0.6253920883499013 2.040571500770149 7 0.4266409844330712 1.6240851541475703 8 0.2746021286419641 1.1946538034746228 9 0.2070049811838175 1.0131447286989625 >10 1.039572030618712 10.136995866247672 >50 0.06902267642350035 2.5729861483911263 >100 0.03585938426697982 3.5475582836055 >500 0.002350317343727533 0.8776786634679102 >1k 0.0013033575465040508 1.0968779961682495 >5k 8.688990086210892E-5 0.30371506007840937 >10k+ 5.213394051726536E-5 0.8133719519207336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35331 0.33343286647132503 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30217 0.28516999026815054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20520 0.19365549857042227 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 9.43740246444553E-6 0.0 0.0 0.0 4 0.0 9.43740246444553E-6 0.0 9.43740246444553E-6 0.0 5 0.0 9.43740246444553E-6 0.0 9.43740246444553E-6 0.0 6 0.0 9.43740246444553E-6 0.0 9.43740246444553E-6 0.0 7 0.0 9.43740246444553E-6 0.0 9.43740246444553E-6 0.0 8 0.0 9.43740246444553E-6 0.0 9.43740246444553E-6 0.0 9 0.0 9.43740246444553E-6 0.0 2.831220739333659E-5 0.0 10-11 0.0 9.43740246444553E-6 0.0 4.718701232222765E-5 0.0 12-13 0.0 9.43740246444553E-6 0.0 4.718701232222765E-5 0.0 14-15 0.0 9.43740246444553E-6 0.0 5.662441478667318E-5 0.0 16-17 0.0 9.43740246444553E-6 0.0 6.134311601889595E-5 0.0 18-19 4.718701232222765E-6 9.43740246444553E-6 0.0 6.606181725111871E-5 0.0 20-21 9.43740246444553E-6 9.43740246444553E-6 0.0 8.0217920947787E-5 0.0 22-23 9.43740246444553E-6 9.43740246444553E-6 0.0 1.9818545175335613E-4 0.0 24-25 9.43740246444553E-6 9.43740246444553E-6 0.0 4.3412051336449433E-4 0.0 26-27 9.43740246444553E-6 9.43740246444553E-6 0.0 8.163353131745383E-4 0.0 28-29 9.43740246444553E-6 2.831220739333659E-5 0.0 0.0017412007546902001 0.0 30-31 9.43740246444553E-6 2.831220739333659E-5 0.0 0.004345923834877166 0.0 32-33 9.43740246444553E-6 2.831220739333659E-5 0.0 0.009786586355630015 0.0 34-35 9.43740246444553E-6 3.3030908625559355E-5 0.0 0.019516548296473354 0.0 36-37 9.43740246444553E-6 3.774960985778212E-5 0.0 0.035064668856647364 0.0 38-39 9.43740246444553E-6 4.718701232222765E-5 0.0 0.0558222355771953 0.0 40-41 9.43740246444553E-6 4.718701232222765E-5 0.0 0.07973189472086806 0.0 42-43 9.43740246444553E-6 4.718701232222765E-5 0.0 0.10448620138510868 0.0 44-45 9.43740246444553E-6 4.718701232222765E-5 0.0 0.13488879342431997 0.0 46-47 9.43740246444553E-6 4.718701232222765E-5 0.0 0.17216181445764758 0.0 48-49 9.43740246444553E-6 4.718701232222765E-5 0.0 0.21150634533192098 0.0 50-51 9.43740246444553E-6 4.718701232222765E-5 0.0 0.2532904447432536 0.0 52-53 9.43740246444553E-6 4.718701232222765E-5 0.0 0.2940033989748716 0.0 54-55 9.43740246444553E-6 5.662441478667318E-5 0.0 0.34160565700553486 0.0 56-57 9.43740246444553E-6 8.0217920947787E-5 0.0 0.3989331582758092 0.0 58-59 9.43740246444553E-6 8.493662218000977E-5 0.0 0.46137573168181306 0.0 60-61 9.43740246444553E-6 8.493662218000977E-5 0.0 0.5289145024186175 0.0 62-63 9.43740246444553E-6 8.493662218000977E-5 0.0 0.5990627149368412 0.0 64-65 9.43740246444553E-6 8.493662218000977E-5 0.0 0.6789267332922114 0.0 66-67 9.43740246444553E-6 8.493662218000977E-5 0.0 0.77311672858861 0.0 68-69 9.43740246444553E-6 1.085301283411236E-4 0.0 0.878839229696561 0.0 70-71 9.43740246444553E-6 1.1796753080556913E-4 0.0 0.99502309143635 4.718701232222765E-6 72-73 9.43740246444553E-6 1.226862320377919E-4 0.0 1.1230367371653214 9.43740246444553E-6 74-75 9.43740246444553E-6 1.3684233573446017E-4 0.0 1.257345130338078 9.43740246444553E-6 76-77 9.43740246444553E-6 1.60435841895574E-4 0.0 1.4013929228541424 9.43740246444553E-6 78-79 9.43740246444553E-6 1.6987324436001954E-4 0.0 1.5689917532202307 9.43740246444553E-6 80-81 9.43740246444553E-6 1.6987324436001954E-4 0.0 1.7554134828016554 9.43740246444553E-6 82-83 9.43740246444553E-6 1.745919455922423E-4 0.0 1.9526740691134958 9.43740246444553E-6 84-85 9.43740246444553E-6 1.7931064682446507E-4 0.0 2.1559415620939557 9.43740246444553E-6 86-87 9.43740246444553E-6 1.8402934805668785E-4 0.0 2.378952101030036 9.43740246444553E-6 88-89 9.43740246444553E-6 1.887480492889106E-4 0.0 2.630491907616135 9.43740246444553E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18975 0.0 54.87065 1 GTATCAA 34595 0.0 40.109665 1 TACCTGG 9455 0.0 32.4036 2 ATCAACG 42650 0.0 32.084908 3 GTACCTG 9855 0.0 31.873102 1 TCAACGC 43355 0.0 31.607143 4 CAACGCA 44800 0.0 30.725504 5 TATCAAC 45140 0.0 30.50575 2 GTGGTAT 8435 0.0 29.06994 1 AACGCAG 48495 0.0 28.707098 6 TGGTATC 8650 0.0 26.578123 2 TATAACG 1200 0.0 25.72925 2 ATAACGC 1230 0.0 25.100643 3 CGCAGAG 54925 0.0 25.077488 8 ACGCAGA 55825 0.0 24.766783 7 GCAGAGT 60390 0.0 22.430586 9 ACCTGGG 13235 0.0 22.358397 3 GTACATG 27990 0.0 21.47668 1 TACATGG 27135 0.0 21.25122 2 AGAGTAC 55590 0.0 20.441387 10-11 >>END_MODULE