FastQCFastQC Report
Wed 25 May 2016
SRR2049418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081789
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637490.7887981237817518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450380.557277602768397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309270.38267517253914946No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167160.20683539251024743No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162300.2008218724839265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108600.1343761882424795No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.116954303063344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93250.11538286881778279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227900.057.9850431
TATAACG8200.049.2352372
GTATCAA466400.047.9491731
TACCTGG74800.041.2745862
GTACCTG82200.038.3128931
ATCAACG616350.035.9977153
TATCAAC621300.035.716792
TCAACGC622650.035.6040734
GTGGTAT84850.034.9330061
CAACGCA644500.034.4111185
AACGCAG679450.032.7808766
TGGTATC89700.032.4063382
ACCTGGG101900.029.5534043
CGCAGAG754300.029.069738
ACGCAGA758800.029.0599237
ATAACGC15600.028.9262053
GTACACG8500.028.500631
GTACATG530050.027.7541261
GCAGAGT787650.027.175489
TACATGG514350.027.1216552