##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8081789 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48506475484574 31.0 31.0 34.0 30.0 34.0 2 31.4796152188581 31.0 31.0 34.0 30.0 34.0 3 31.49840053978148 33.0 31.0 34.0 28.0 34.0 4 35.13142461403039 37.0 35.0 37.0 32.0 37.0 5 35.18018757975493 37.0 35.0 37.0 33.0 37.0 6 35.29470170527837 37.0 35.0 37.0 33.0 37.0 7 35.244826238348956 37.0 35.0 37.0 33.0 37.0 8 35.22117862765286 37.0 35.0 37.0 33.0 37.0 9 36.79542153352432 39.0 37.0 39.0 33.0 39.0 10-11 36.718500384011506 39.0 37.0 39.0 32.5 39.0 12-13 36.67847830474169 39.0 37.0 39.0 33.0 39.0 14-15 37.80357603990899 40.0 38.0 41.0 32.5 41.0 16-17 37.80929618429781 40.0 38.0 41.0 33.0 41.0 18-19 37.88380740947332 40.0 38.0 41.0 33.0 41.0 20-21 37.85888328685641 40.0 38.0 41.0 33.0 41.0 22-23 37.73384655303424 40.0 38.0 41.0 32.5 41.0 24-25 37.61477427831882 40.0 37.5 41.0 32.0 41.0 26-27 37.46772447288589 40.0 37.0 41.0 32.0 41.0 28-29 37.307098527318644 40.0 37.0 41.0 32.0 41.0 30-31 37.05183790371167 40.0 36.5 41.0 31.5 41.0 32-33 36.732059077513654 39.0 35.5 41.0 31.0 41.0 34-35 36.49953939653708 39.0 35.0 41.0 30.0 41.0 36-37 36.28027785679631 39.0 35.0 41.0 30.0 41.0 38-39 36.043925917887734 39.0 35.0 40.5 30.0 41.0 40-41 35.80108500729232 39.0 35.0 40.0 29.5 41.0 42-43 35.51979444155248 39.0 35.0 40.0 27.5 41.0 44-45 35.14076357351077 38.0 35.0 40.0 26.5 41.0 46-47 34.641487423143566 38.0 34.5 40.0 23.5 41.0 48-49 34.21621178182207 38.0 34.0 40.0 22.0 41.0 50-51 32.80976433559451 36.5 32.0 39.0 18.5 40.0 52-53 33.07876690420896 37.5 32.5 39.0 19.0 40.0 54-55 34.079435629908176 38.0 34.0 40.0 20.5 41.0 56-57 34.16319790086081 38.0 34.0 40.0 20.0 41.0 58-59 33.98166804404322 38.0 34.0 40.0 19.5 41.0 60-61 33.70394921223506 38.0 34.0 40.0 18.0 41.0 62-63 33.41325961368207 37.0 34.0 40.0 15.0 41.0 64-65 33.1187419641864 37.0 33.0 40.0 12.5 41.0 66-67 32.774562971144135 36.0 33.0 39.5 8.5 41.0 68-69 32.403872397559496 36.0 33.0 39.0 7.0 41.0 70-71 32.043412665190836 35.0 32.0 39.0 4.0 40.5 72-73 31.765444631133033 35.0 32.0 38.0 2.0 40.0 74-75 31.55254845183412 35.0 32.0 37.5 2.0 40.0 76-77 31.220769003001685 35.0 32.0 37.0 2.0 39.0 78-79 30.849251013606022 35.0 32.0 36.5 2.0 39.0 80-81 30.5267535442957 35.0 31.0 36.0 2.0 38.5 82-83 30.197675168208427 35.0 31.0 36.0 2.0 37.0 84-85 29.899523360483677 35.0 31.0 35.0 2.0 37.0 86-87 29.62022560846367 35.0 31.0 35.0 2.0 36.5 88-89 29.341529579651237 34.5 31.0 35.0 2.0 36.0 90-91 29.087083194079923 34.0 30.0 35.0 2.0 36.0 92-93 28.845043714949746 34.0 30.0 35.0 2.0 36.0 94-95 28.578976201927567 34.0 30.0 35.0 2.0 35.0 96-97 28.322424341937165 34.0 29.0 35.0 2.0 35.0 98-99 28.00507511393826 34.0 29.0 35.0 2.0 35.0 100-101 27.47728782575244 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24680901312267167 1101 2 0.24511457331351139 1101 3 0.2410719635048686 1101 4 0.21515069411378107 1101 5 0.22027531089234031 1101 6 0.22469540622370943 1101 7 0.20681071938733453 1101 8 0.20348195671683555 1101 9 0.2540934940430333 1101 10-11 0.26510172853742375 1101 12-13 0.27338610843884226 1101 14-15 0.28574883861826805 1101 16-17 0.32451939420310083 1101 18-19 0.26298683737945083 1101 20-21 0.24616250466949907 1101 22-23 0.25217865669547024 1101 24-25 0.22649369005169007 1101 26-27 0.20296449918311055 1101 28-29 0.17811431308407322 1101 30-31 0.24441423007219498 1101 32-33 0.18874180543866714 1101 34-35 0.2098251700070506 1101 36-37 0.1715031344098179 1101 38-39 0.06459214345441211 1101 40-41 -0.02119667426757843 1101 42-43 -0.036053321802903326 1101 44-45 -0.0402964732044353 1101 46-47 -0.06321894312723231 1101 48-49 -0.12865382339251852 1101 50-51 -0.16064440600552388 1101 52-53 -0.09012189129288828 1101 54-55 0.1480076917197053 1101 56-57 0.14713732285694192 1101 58-59 0.04290340399926862 1101 60-61 0.0904849512479231 1101 62-63 0.04280920074324257 1101 64-65 0.004816718683088084 1101 66-67 0.007988522605245407 1101 68-69 -0.0435689481394661 1101 70-71 -0.0664305275807422 1101 72-73 -0.14512238112055798 1101 74-75 -0.12391377216126287 1101 76-77 -0.1986490073349252 1101 78-79 -0.20712626039363968 1101 80-81 -0.3206173759147113 1101 82-83 -0.31394612862726134 1101 84-85 -0.48414414263866234 1101 86-87 -0.4724173192969623 1101 88-89 -0.4443668038850461 1101 90-91 -0.46968473941903355 1101 92-93 -0.5561958589749523 1101 94-95 -0.6580626312229434 1101 96-97 -0.7112998075478885 1101 98-99 -0.8893031463938144 1101 100-101 -1.019186688056898 1102 1 0.26310488074445004 1102 2 0.29916137859619596 1102 3 0.31479387409042303 1102 4 0.27842339867802934 1102 5 0.24198965212706014 1102 6 0.30157254022966384 1102 7 0.2880736663566168 1102 8 0.2807878742666503 1102 9 0.4036899280559041 1102 10-11 0.41313725194125794 1102 12-13 0.3610233799196223 1102 14-15 0.419189896748307 1102 16-17 0.3754202582362254 1102 18-19 0.3640561162356093 1102 20-21 0.39017039119662655 1102 22-23 0.3627651044197435 1102 24-25 0.3473788211880873 1102 26-27 0.3435781400095479 1102 28-29 0.37008834070223173 1102 30-31 0.36358886386460654 1102 32-33 0.3703825308058555 1102 34-35 0.3816099995877593 1102 36-37 0.2825565578547895 1102 38-39 0.26273184667677896 1102 40-41 0.23469749571236775 1102 42-43 0.20782771122529198 1102 44-45 0.2371433849646749 1102 46-47 0.18898812908720686 1102 48-49 0.07394307447366089 1102 50-51 0.0032171165972059157 1102 52-53 0.1698993649634346 1102 54-55 0.30704369361050965 1102 56-57 0.3017065246310935 1102 58-59 0.307776274842837 1102 60-61 0.30471479991876294 1102 62-63 0.23712100817503057 1102 64-65 0.18064832802263453 1102 66-67 0.17763370545635127 1102 68-69 0.1726360971415275 1102 70-71 0.09015974565709683 1102 72-73 0.16599490297921093 1102 74-75 0.16065184096057905 1102 76-77 0.18738369925761234 1102 78-79 0.0862996144530861 1102 80-81 0.07208165645938891 1102 82-83 0.11426667658259859 1102 84-85 0.0233056800386926 1102 86-87 -0.07491254718914675 1102 88-89 -0.06224408626960809 1102 90-91 -0.09690203818650289 1102 92-93 -0.1859744668637262 1102 94-95 -0.3063845916991035 1102 96-97 -0.37569649707144137 1102 98-99 -0.33658381916147206 1102 100-101 -0.38611261173924305 1103 1 0.24288018299739278 1103 2 0.2559427033881647 1103 3 0.30290311497662614 1103 4 0.24550549635338825 1103 5 0.2366338542259001 1103 6 0.2818100833524042 1103 7 0.2591822451878869 1103 8 0.27063426086039044 1103 9 0.3290569025273484 1103 10-11 0.36669362983689524 1103 12-13 0.38142693403252537 1103 14-15 0.42540814941794025 1103 16-17 0.43259627112429655 1103 18-19 0.39593856516716386 1103 20-21 0.3928942083299205 1103 22-23 0.3511362514253591 1103 24-25 0.39853730667299914 1103 26-27 0.4320854534645733 1103 28-29 0.37707922944133543 1103 30-31 0.3201381570174817 1103 32-33 0.25784201150798225 1103 34-35 0.3081955469520139 1103 36-37 0.38451994905479836 1103 38-39 0.402063168591944 1103 40-41 0.3068248724257927 1103 42-43 0.2839019688997766 1103 44-45 0.2608213838873752 1103 46-47 0.29483744819637536 1103 48-49 0.283912116962064 1103 50-51 0.2430275106253319 1103 52-53 0.2718136162230351 1103 54-55 0.41438748522487856 1103 56-57 0.44063431326087965 1103 58-59 0.44529771743007274 1103 60-61 0.4536183218791692 1103 62-63 0.3807230139697708 1103 64-65 0.3753362483969269 1103 66-67 0.40066258203790994 1103 68-69 0.3065368373257158 1103 70-71 0.3306167041460242 1103 72-73 0.3849296414935459 1103 74-75 0.35189137415327565 1103 76-77 0.2923929857300571 1103 78-79 0.355194800196859 1103 80-81 0.30613512748348626 1103 82-83 0.2672448433491077 1103 84-85 0.28567560238587575 1103 86-87 0.2327169199568644 1103 88-89 0.18700277092204232 1103 90-91 0.1513023688655899 1103 92-93 0.1258928808109232 1103 94-95 0.1556596857953778 1103 96-97 0.14287153382410622 1103 98-99 0.01901059057476928 1103 100-101 -0.01650352354483431 1104 1 0.05259161303788318 1104 2 0.06925995886849279 1104 3 0.08345661211243183 1104 4 0.036744251456610755 1104 5 -0.005039082752738011 1104 6 0.04388358649047319 1104 7 0.011438575288032382 1104 8 -0.04115714477562449 1104 9 -0.011587918739927261 1104 10-11 0.004378448787427658 1104 12-13 -0.049491464209786784 1104 14-15 -0.06815270910848881 1104 16-17 -0.014957876298943518 1104 18-19 -0.09387162339591981 1104 20-21 0.002077275078356422 1104 22-23 -0.0024501103831084947 1104 24-25 0.008569305225151425 1104 26-27 -0.02110451309840755 1104 28-29 -0.08306731399135714 1104 30-31 -0.07124476773485355 1104 32-33 -0.12109309244232236 1104 34-35 -0.13065611083735718 1104 36-37 -0.10060076538182727 1104 38-39 -0.1435431011877384 1104 40-41 -0.14208426755354964 1104 42-43 -0.1270763144656044 1104 44-45 -0.2015565858293371 1104 46-47 -0.15776211415548858 1104 48-49 -0.16546761325799508 1104 50-51 -0.24309301598709254 1104 52-53 -0.22885332128054614 1104 54-55 -0.08713464172070218 1104 56-57 -0.1673587883186869 1104 58-59 -0.2459943242354683 1104 60-61 -0.22497942724675823 1104 62-63 -0.1714665130693689 1104 64-65 -0.18793444708617812 1104 66-67 -0.16283332505475556 1104 68-69 -0.19788991104574194 1104 70-71 -0.21378927140109028 1104 72-73 -0.27410845716282495 1104 74-75 -0.2742792953107305 1104 76-77 -0.1879925500574835 1104 78-79 -0.16339710255228468 1104 80-81 -0.17830792433280251 1104 82-83 -0.27147259047549355 1104 84-85 -0.25382259459051326 1104 86-87 -0.22810023635177856 1104 88-89 -0.2966305425009139 1104 90-91 -0.3159262052027323 1104 92-93 -0.3169715231697836 1104 94-95 -0.3384815013524296 1104 96-97 -0.36403463341268605 1104 98-99 -0.39570827132209985 1104 100-101 -0.4947800926531727 1105 1 0.24214112651470998 1105 2 0.2658322174634016 1105 3 0.23873155130591073 1105 4 0.2781301716051843 1105 5 0.26159947021531593 1105 6 0.2553376468610935 1105 7 0.28197825890953254 1105 8 0.3147381416362265 1105 9 0.34464175029936683 1105 10-11 0.3496647186533792 1105 12-13 0.21038027768942413 1105 14-15 0.3327308845605401 1105 16-17 0.3001796465434339 1105 18-19 0.3111456068936107 1105 20-21 0.33449123367170586 1105 22-23 0.2779428566486075 1105 24-25 0.32091640149184997 1105 26-27 0.3681292892225301 1105 28-29 0.3911813190155371 1105 30-31 0.3587918794652083 1105 32-33 0.40374848402622376 1105 34-35 0.46126645438145175 1105 36-37 0.3951342587491098 1105 38-39 0.4332722557071449 1105 40-41 0.3905173286797776 1105 42-43 0.41198575291356576 1105 44-45 0.41850417099711024 1105 46-47 0.38343155373085835 1105 48-49 0.3446068170491401 1105 50-51 0.24284374522963503 1105 52-53 0.3734869311463882 1105 54-55 0.514557788736937 1105 56-57 0.5507428128842449 1105 58-59 0.5603448888365463 1105 60-61 0.5517420753310489 1105 62-63 0.5087387453281806 1105 64-65 0.48156737249487236 1105 66-67 0.38914542496476656 1105 68-69 0.44768580212637943 1105 70-71 0.4281575738301555 1105 72-73 0.47583865216680366 1105 74-75 0.4256641223210984 1105 76-77 0.44987319425268524 1105 78-79 0.45473367726208025 1105 80-81 0.4533039419198275 1105 82-83 0.4385370931318029 1105 84-85 0.40131248605178627 1105 86-87 0.3989942696919577 1105 88-89 0.3963384060609556 1105 90-91 0.40374436756417964 1105 92-93 0.43509618256782545 1105 94-95 0.4342820752211942 1105 96-97 0.3792269531938359 1105 98-99 0.4335049142865266 1105 100-101 0.40484335417146866 1106 1 0.24179074473808626 1106 2 0.27886080211196784 1106 3 0.26803103782737736 1106 4 0.24105865861374554 1106 5 0.2392654884123786 1106 6 0.2824252040549098 1106 7 0.3589915697829511 1106 8 0.3287587098503266 1106 9 0.38779337417122406 1106 10-11 0.38020346089336243 1106 12-13 0.39267250255857533 1106 14-15 0.43218655321228283 1106 16-17 0.4745250390215361 1106 18-19 0.48058183562401524 1106 20-21 0.4906184830198512 1106 22-23 0.5132091443350504 1106 24-25 0.5140758663763094 1106 26-27 0.6125529464629906 1106 28-29 0.5658270475299929 1106 30-31 0.6173253249256447 1106 32-33 0.6665542804378219 1106 34-35 0.6841754786786183 1106 36-37 0.7218107232938067 1106 38-39 0.6367425597659704 1106 40-41 0.6320581049018372 1106 42-43 0.6623938489777288 1106 44-45 0.6819666907105102 1106 46-47 0.6834280181917904 1106 48-49 0.5941205726367045 1106 50-51 0.6672228146412564 1106 52-53 0.7180584589205736 1106 54-55 0.6953940829302496 1106 56-57 0.7779057405027672 1106 58-59 0.7550235730099288 1106 60-61 0.728349442877068 1106 62-63 0.7452865735339955 1106 64-65 0.8030551716821677 1106 66-67 0.7697543821482782 1106 68-69 0.7151133611483189 1106 70-71 0.7381236425102813 1106 72-73 0.7305313103427586 1106 74-75 0.7392558123999358 1106 76-77 0.71620308850434 1106 78-79 0.7193835591110194 1106 80-81 0.7040514558938291 1106 82-83 0.720855739072082 1106 84-85 0.717136462525545 1106 86-87 0.6881406482774786 1106 88-89 0.7407545456612858 1106 90-91 0.6825836180594642 1106 92-93 0.6975026293707742 1106 94-95 0.7491347023731016 1106 96-97 0.8273325694777149 1106 98-99 0.8493687856845575 1106 100-101 0.8588509185004156 1107 1 0.25519741061905776 1107 2 0.3035829130807315 1107 3 0.27886154229074833 1107 4 0.3016365697261989 1107 5 0.27993396229070555 1107 6 0.34850548422058125 1107 7 0.27882700873614397 1107 8 0.3584443570259026 1107 9 0.4068940109991388 1107 10-11 0.39986348647324377 1107 12-13 0.4013336365486495 1107 14-15 0.5547832401484598 1107 16-17 0.48194733710266036 1107 18-19 0.48836553035798147 1107 20-21 0.5006716912366329 1107 22-23 0.49281595812321655 1107 24-25 0.4795469052503236 1107 26-27 0.500883533149981 1107 28-29 0.5200960935298227 1107 30-31 0.5464211472029774 1107 32-33 0.58708961818445 1107 34-35 0.5962023100340375 1107 36-37 0.6294251178020289 1107 38-39 0.6308111830052994 1107 40-41 0.6198877948151065 1107 42-43 0.5788471264768518 1107 44-45 0.6211869295427306 1107 46-47 0.6467975434242561 1107 48-49 0.47720459213014266 1107 50-51 0.4884403284157983 1107 52-53 0.5693518767170289 1107 54-55 0.6347617880415442 1107 56-57 0.5362424933656058 1107 58-59 0.5885997217364931 1107 60-61 0.6499644050857754 1107 62-63 0.6244615822240789 1107 64-65 0.5880423358597398 1107 66-67 0.6522312576482463 1107 68-69 0.6410562128708861 1107 70-71 0.6713733440921601 1107 72-73 0.6217776107266282 1107 74-75 0.671411388668961 1107 76-77 0.6384649212283655 1107 78-79 0.6424414639649392 1107 80-81 0.6788632323185055 1107 82-83 0.6202256737543514 1107 84-85 0.6889623298149523 1107 86-87 0.695435589117583 1107 88-89 0.7129546198055436 1107 90-91 0.7833272026209208 1107 92-93 0.7708708122132322 1107 94-95 0.8064732857840191 1107 96-97 0.8237451766708048 1107 98-99 0.849745271563723 1107 100-101 0.9001116854752169 1108 1 0.09040417070027829 1108 2 0.15655900513792886 1108 3 0.16154192137566525 1108 4 0.07921245902173268 1108 5 0.11235049886457915 1108 6 0.11241111071402798 1108 7 0.17833363070575103 1108 8 0.1553551931683188 1108 9 0.1506126667423402 1108 10-11 0.1728636166930002 1108 12-13 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-1.0230951659649499 2316 96-97 -0.7734355720509427 2316 98-99 -0.5800413338142114 2316 100-101 -0.5315463479595977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69109.0 3 14943.0 4 12116.0 5 10653.0 6 11565.0 7 13049.0 8 14819.0 9 17820.0 10 21895.0 11 27601.0 12 37126.0 13 45932.0 14 48735.0 15 61511.0 16 81767.0 17 110693.0 18 87809.0 19 47022.0 20 39202.0 21 38302.0 22 39471.0 23 43123.0 24 49182.0 25 57161.0 26 68132.0 27 81758.0 28 98942.0 29 120051.0 30 149423.0 31 188975.0 32 245853.0 33 328660.0 34 465228.0 35 731115.0 36 1111851.0 37 1642921.0 38 1536884.0 39 311169.0 40 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.466058864236444 19.989057873232703 13.85329927026088 20.691583992269972 2 16.47235510219284 20.914374510374824 40.18708553936633 22.426184848066004 3 18.769284148882818 28.051364094258908 29.86262478124266 23.316726975615616 4 12.061340877964177 17.241984365943633 39.28792926710597 31.408745488986224 5 11.76755113578783 38.861429293883575 34.55433355717595 14.81668601315264 6 28.243097258950083 38.58422191726239 18.639535416107243 14.533145407680287 7 26.325993847314304 34.02788828156147 22.488484440228596 17.15763343089563 8 26.625027346530285 33.21218779171694 20.680139143105755 19.482645718647017 9 27.120779675917127 15.828430411677122 22.127373004181713 34.923416908224034 10-11 24.06437070174796 27.102969462920818 28.320265129203708 20.512394706127512 12-13 25.277727471648742 25.644831936727257 28.630850448984006 20.446590142639995 14-15 22.572411503925167 26.233574759149057 28.179728955104572 23.0142847818212 16-17 20.148505965909038 29.749817835428903 28.31228770797341 21.789388490688648 18-19 20.11924217830891 27.422081612185433 32.50710191020735 19.9515742992983 20-21 21.89249742907546 26.363982036998795 32.27707136213404 19.46644917179171 22-23 21.18663792191427 26.474908085630922 32.64361105890129 19.69484293355352 24-25 20.34090129362667 27.009976432323658 32.56903635111909 20.080085922930586 26-27 19.888967653954943 27.161058584339752 32.541523756328964 20.408450005376345 28-29 20.33674584399163 26.930207914564857 32.679711146111856 20.053335095331658 30-31 20.5433083395593 27.361160570995423 32.22927322882951 19.866257860615764 32-33 19.863975199240556 27.09425308178193 32.66759370072193 20.37417801825559 34-35 20.45190633251545 26.943449097307777 32.490832140865635 20.11381242931114 36-37 20.910156730929256 27.127412167140886 31.788587404730574 20.173843697199285 38-39 20.627363340536277 27.354511891973527 31.955324461339785 20.06280030615041 40-41 20.35693685490176 27.191259074119206 32.10000235721539 20.351801713763646 42-43 20.384829642139106 27.280297803299753 31.7355921666764 20.59928038788474 44-45 20.656243698692002 27.576537748079343 31.11935236521442 20.647866188014234 46-47 21.12242908463511 27.831891926776454 30.525858104115027 20.519820884473404 48-49 21.286518888019675 27.773887483873395 30.155650665988894 20.783942962118036 50-51 21.242286028730685 28.050162846981376 29.53915736578728 21.16839375850066 52-53 21.089400969676706 28.200270656089494 29.587830165686054 21.12249820854775 54-55 21.187368083945156 28.755477186461526 29.392405161973873 20.664749567619445 56-57 21.12323819833251 28.413300091395534 29.332452540773467 21.131009169498494 58-59 21.29008124167789 27.956184052273542 29.523514190271232 21.230220515777336 60-61 21.483289090271622 28.254175817247933 29.316850707368264 20.94568438511218 62-63 20.951159245747526 28.259843884700427 29.56964167080467 21.219355198747376 64-65 21.120783685138104 28.17172141254475 29.493827531706852 21.2136673706103 66-67 20.980314735562782 28.096814755732108 29.32938344466885 21.593487064036257 68-69 21.510898217107584 28.683486761293466 28.92160665844804 20.884008363150908 70-71 21.636399733988963 28.286873689116394 28.92555755687335 21.151169020021296 72-73 21.284065074914306 28.26868997009417 29.13600119578787 21.311243759203663 74-75 21.21378997162715 28.507431013639504 29.120701538172185 21.158077476561157 76-77 21.399786714158118 28.762547372448605 28.626502782887737 21.211163130505543 78-79 21.469514331191473 28.691654264561013 28.836624949311922 21.002206454935592 80-81 21.32035467749133 28.63925949960932 28.851651679300303 21.188734143599042 82-83 21.149335957718606 28.639136403166958 29.153836848584376 21.057690790530064 84-85 21.113982392171042 28.58026391696854 28.90985695402329 21.39589673683713 86-87 21.287240937851813 28.849047969884285 28.61993436254928 21.243776729714618 88-89 21.104438706267022 28.673734870465346 28.966376969549383 21.25544945371825 90-91 20.893516811674562 28.53934411147634 28.93724320820524 21.629895868643864 92-93 21.092980274804145 28.836468526283298 28.78758041528167 21.282970783630883 94-95 21.045534302195836 28.41667814840151 28.995425639717205 21.542361909685447 96-97 21.11866580776658 28.799488322563672 28.582335992112505 21.499509877557244 98-99 20.895240735080662 28.548598057983316 28.745058029101788 21.81110317783423 100-101 20.98247904896537 28.75553672657915 28.498499336493076 21.7634848879624 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15729.0 1 10253.0 2 10198.5 3 13252.0 4 8255.0 5 5966.0 6 8877.0 7 15866.0 8 23431.0 9 24735.5 10 18876.5 11 13972.0 12 13391.0 13 14025.0 14 14560.0 15 15006.0 16 15694.0 17 16728.5 18 17799.0 19 19195.5 20 21144.5 21 23678.0 22 27635.0 23 34346.0 24 41073.5 25 47788.0 26 57987.5 27 72112.5 28 92791.5 29 110576.0 30 121028.0 31 137535.0 32 160297.5 33 179978.0 34 198738.0 35 221971.5 36 242593.5 37 258807.0 38 277260.5 39 288796.0 40 296737.5 41 298295.5 42 292807.0 43 295840.0 44 295837.0 45 292893.5 46 291203.5 47 282676.5 48 270794.5 49 267882.5 50 262939.0 51 243416.5 52 220667.5 53 197683.0 54 179901.0 55 172092.0 56 157591.0 57 138699.5 58 124729.0 59 109647.0 60 93764.0 61 78796.0 62 65731.5 63 53824.5 64 43884.0 65 35524.0 66 28304.0 67 21907.5 68 16182.5 69 11856.0 70 8815.0 71 6429.0 72 4718.0 73 3558.5 74 2767.5 75 2175.5 76 1560.0 77 1179.0 78 939.0 79 687.5 80 512.0 81 410.5 82 316.5 83 243.5 84 208.0 85 184.5 86 152.0 87 130.0 88 116.0 89 94.0 90 81.5 91 69.5 92 52.0 93 45.5 94 40.5 95 38.0 96 30.5 97 19.5 98 12.5 99 7.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013524233310223762 2 0.004355471294783865 3 0.004281230306804595 4 0.005654688584421098 5 0.002697422563246826 6 0.007164255339999597 7 0.0014229522696026832 8 0.004021386848877148 9 0.01104953371091475 10-11 0.007386978303937409 12-13 0.008791370326545273 14-15 0.0035450071760101632 16-17 0.006155815253281173 18-19 0.004163682075837416 20-21 8.166508677719748E-4 22-23 0.001014626835716696 24-25 0.0073436710609495 26-27 0.0011136148196890566 28-29 6.92915887806524E-4 30-31 5.320604138514381E-4 32-33 0.0012497232976510524 34-35 0.0019611994324523936 36-37 0.0012868437916406874 38-39 0.0017384764685145827 40-41 0.002715982810241643 42-43 0.0026355550732641004 44-45 0.007789116988825123 46-47 0.013394311581260039 48-49 0.005233989652538565 50-51 0.005314417389516109 52-53 0.013047853637356777 54-55 0.008110827936735295 56-57 0.0053329776365109265 58-59 0.006564140687167161 60-61 0.0013301510346285952 62-63 0.002449952603315924 64-65 0.0021777356473919327 66-67 0.0026108080772710104 68-69 0.0018065307074955806 70-71 0.00209730791041439 72-73 0.0010888678236959663 74-75 0.0014724462615888635 76-77 0.0076097012678752185 78-79 0.0061867489982725355 80-81 0.012163148530603806 82-83 0.014433685412969827 84-85 0.007560207275889038 86-87 0.005283483644524746 88-89 0.0030315070091535426 90-91 0.0026912358142485533 92-93 8.599581107598825E-4 94-95 0.0013920185246113206 96-97 0.0016704222295335848 98-99 0.00617437550027599 100-101 0.010684515520016669 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8081789.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.04233544840376 #Duplication Level Percentage of deduplicated Percentage of total 1 68.27720283589653 30.0708747077726 2 17.25950532314324 15.202978462307698 3 6.396513122012203 8.451521298593336 4 3.0118902361469106 5.3060272045661705 5 1.562665621410148 3.4411721745917023 6 0.8734811930688785 2.3082091027806886 7 0.5883634918994505 1.8139031593080852 8 0.3740488419484201 1.3179187656943423 9 0.24086892979183597 0.9547587184491048 >10 1.1992559178722646 9.38807831408828 >50 0.1052477147922122 3.2615493298405545 >100 0.09456805261387631 8.529419420612342 >500 0.010513941543381151 3.116344889613491 >1k 0.005621796999143755 4.255364148237978 >5k 8.43269538301997E-5 0.30992962719290135 >10k+ 1.686539076603994E-4 2.271950676350759 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63749 0.7887981237817518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45038 0.557277602768397 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30927 0.38267517253914946 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16716 0.20683539251024743 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16230 0.2008218724839265 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10860 0.1343761882424795 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9452 0.116954303063344 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9325 0.11538286881778279 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2373497996545072E-5 0.0 5 0.0 0.0 0.0 1.2373497996545072E-5 0.0 6 0.0 0.0 0.0 1.2373497996545072E-5 0.0 7 0.0 0.0 0.0 2.4746995993090144E-5 0.0 8 0.0 0.0 0.0 2.4746995993090144E-5 0.0 9 0.0 0.0 0.0 2.4746995993090144E-5 0.0 10-11 0.0 0.0 0.0 4.949399198618029E-5 0.0 12-13 0.0 0.0 0.0 8.66144859758155E-5 0.0 14-15 0.0 2.4746995993090144E-5 0.0 8.66144859758155E-5 0.0 16-17 0.0 6.186748998272536E-5 0.0 8.66144859758155E-5 0.0 18-19 0.0 7.424098797927044E-5 0.0 8.66144859758155E-5 0.0 20-21 0.0 7.424098797927044E-5 0.0 1.4848197595854087E-4 0.0 22-23 0.0 7.424098797927044E-5 0.0 3.897651868911698E-4 0.0 24-25 0.0 9.898798397236057E-5 0.0 0.001027000333713241 0.0 26-27 0.0 1.1136148196890564E-4 0.0 0.001534313751571589 0.0 28-29 0.0 1.1136148196890564E-4 0.0 0.003012946762158725 0.0 30-31 0.0 1.1136148196890564E-4 0.0 0.0068982251330738775 0.0 32-33 0.0 1.1754823096717817E-4 0.0 0.014155281708047562 0.0 34-35 0.0 1.361084779619958E-4 0.0 0.02432011031220934 0.0 36-37 0.0 1.4848197595854087E-4 0.0 0.0400530130148164 0.0 38-39 0.0 1.4848197595854087E-4 0.0 0.06455253904797564 0.0 40-41 0.0 1.8560246994817607E-4 0.0 0.09467581992056462 0.0 42-43 0.0 1.8560246994817607E-4 0.0 0.12282552786270465 0.0 44-45 0.0 1.917892189464486E-4 0.0 0.15632677368835043 0.0 46-47 0.0 2.041627169429937E-4 0.0 0.19770993773779544 0.0 48-49 0.0 2.1034946594126623E-4 0.0 0.2435599345639932 0.0 50-51 0.0 2.1034946594126623E-4 0.0 0.2936602279520042 0.0 52-53 0.0 2.1034946594126623E-4 0.0 0.3421148461064747 0.0 54-55 0.0 2.1034946594126623E-4 0.0 0.39467746559579814 0.0 56-57 0.0 2.1034946594126623E-4 0.0 0.4572069377213387 0.0 58-59 0.0 2.1034946594126623E-4 0.0 0.5295052865151515 0.0 60-61 0.0 2.1034946594126623E-4 0.0 0.6083925229921247 0.0 62-63 0.0 2.1034946594126623E-4 0.0 0.6913630138079576 0.0 64-65 0.0 2.1034946594126623E-4 0.0 0.7775134936089028 0.0 66-67 0.0 2.1034946594126623E-4 0.0 0.8752888747776019 0.0 68-69 0.0 2.1034946594126623E-4 0.0 0.9902139241695125 0.0 70-71 0.0 2.1034946594126623E-4 0.0 1.121997864581716 0.0 72-73 0.0 2.1034946594126623E-4 0.0 1.2637362841321396 0.0 74-75 0.0 2.1034946594126623E-4 0.0 1.4081844997438067 6.186748998272536E-6 76-77 0.0 2.1034946594126623E-4 0.0 1.558423512417857 1.2373497996545072E-5 78-79 0.0 2.1653621493953874E-4 0.0 1.7366266305640992 1.2373497996545072E-5 80-81 0.0 2.2272296393781128E-4 0.0 1.9392859675005125 1.2373497996545072E-5 82-83 0.0 2.2272296393781128E-4 0.0 2.1574554841755953 1.2373497996545072E-5 84-85 0.0 2.2890971293608383E-4 0.0 2.3757920430736315 1.2373497996545072E-5 86-87 0.0 2.3509646193435637E-4 0.0 2.6058463045743956 1.2373497996545072E-5 88-89 0.0 2.3509646193435637E-4 0.0 2.8660176601987506 1.2373497996545072E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22790 0.0 57.985043 1 TATAACG 820 0.0 49.235237 2 GTATCAA 46640 0.0 47.949173 1 TACCTGG 7480 0.0 41.274586 2 GTACCTG 8220 0.0 38.312893 1 ATCAACG 61635 0.0 35.997715 3 TATCAAC 62130 0.0 35.71679 2 TCAACGC 62265 0.0 35.604073 4 GTGGTAT 8485 0.0 34.933006 1 CAACGCA 64450 0.0 34.411118 5 AACGCAG 67945 0.0 32.780876 6 TGGTATC 8970 0.0 32.406338 2 ACCTGGG 10190 0.0 29.553404 3 CGCAGAG 75430 0.0 29.06973 8 ACGCAGA 75880 0.0 29.059923 7 ATAACGC 1560 0.0 28.926205 3 GTACACG 850 0.0 28.50063 1 GTACATG 53005 0.0 27.754126 1 GCAGAGT 78765 0.0 27.17548 9 TACATGG 51435 0.0 27.121655 2 >>END_MODULE