FastQCFastQC Report
Wed 25 May 2016
SRR2049418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081789
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638650.7902334495493509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469190.5805521524998982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377450.46703768187959377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175100.2166599499195042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150750.18653048229791697No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111610.13810061113943953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111430.13787788817550173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96080.11888456875080505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234450.057.242491
GTATCAA460150.046.3095171
TATAACG9450.044.2320632
TACCTGG71500.039.793042
GTACCTG78600.036.1561931
ATCAACG642650.033.148473
TATCAAC649300.032.7293172
ATAACGC14850.032.624913
TCAACGC653600.032.5638584
GTGGTAT89500.031.7528131
CAACGCA673500.031.6445
AACGCAG711900.030.1702616
TGGTATC94150.029.9676342
ACCTGGG94600.029.5230873
CGCAGAG804950.026.4931898
GTATAAC20750.026.338061
ACGCAGA815900.026.224957
GTACATG499250.026.1007771
TACATGG486200.025.5960332
GTACACG7100.025.4348561
Produced by FastQC (version 0.11.5)