##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8081789 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.049358997123036 33.0 31.0 34.0 30.0 34.0 2 32.20742511342476 33.0 31.0 34.0 30.0 34.0 3 32.27241926261623 34.0 31.0 34.0 30.0 34.0 4 35.92528931403678 37.0 35.0 37.0 35.0 37.0 5 35.83082149261754 37.0 35.0 37.0 35.0 37.0 6 35.87723770071206 37.0 35.0 37.0 35.0 37.0 7 35.80245549593042 37.0 35.0 37.0 35.0 37.0 8 35.79148638995648 37.0 35.0 37.0 35.0 37.0 9 37.37931143710879 39.0 37.0 39.0 34.0 39.0 10-11 37.371682000111605 39.0 37.0 39.0 34.0 39.0 12-13 37.357353613166595 39.0 37.0 39.0 34.0 39.0 14-15 38.526984681238275 40.0 38.0 41.0 34.0 41.0 16-17 38.53843425013942 40.0 38.0 41.0 34.0 41.0 18-19 38.61752410264609 40.0 38.0 41.0 34.0 41.0 20-21 38.61414663263295 40.0 38.0 41.0 34.0 41.0 22-23 38.511223443225255 40.0 38.0 41.0 34.0 41.0 24-25 38.40409091353412 40.0 38.0 41.0 34.0 41.0 26-27 38.21956685580383 40.0 38.0 41.0 34.0 41.0 28-29 38.022827804586335 40.0 37.0 41.0 33.0 41.0 30-31 37.763756947873794 40.0 37.0 41.0 33.0 41.0 32-33 37.444137244860016 39.5 36.0 41.0 33.0 41.0 34-35 37.223326357567615 39.0 36.0 41.0 32.0 41.0 36-37 36.99490094334311 39.0 35.0 41.0 31.5 41.0 38-39 37.094317545286074 39.5 36.0 41.0 32.0 41.0 40-41 36.961048908849264 40.0 36.0 41.0 31.5 41.0 42-43 36.702542221777875 39.5 35.0 41.0 31.0 41.0 44-45 36.34920498419348 39.0 35.0 41.0 30.0 41.0 46-47 35.86314335848164 39.0 35.0 41.0 29.0 41.0 48-49 35.4520760811746 39.0 35.0 41.0 27.0 41.0 50-51 35.16708806676344 39.0 35.0 40.0 26.0 41.0 52-53 34.96233680438824 39.0 35.0 40.0 26.0 41.0 54-55 34.82864926317676 38.5 35.0 40.0 25.5 41.0 56-57 34.7006293408551 38.0 35.0 40.0 24.5 41.0 58-59 34.442971104541336 38.0 34.0 40.0 24.0 41.0 60-61 34.17839026730344 38.0 34.0 40.0 23.0 41.0 62-63 33.87346805267992 37.0 34.0 40.0 22.5 41.0 64-65 33.59928717515392 37.0 34.0 40.0 22.0 41.0 66-67 33.24223343371128 36.0 33.0 39.5 21.0 41.0 68-69 32.90123616194385 36.0 33.0 39.0 20.0 41.0 70-71 32.549870146325276 35.0 33.0 39.0 19.0 40.0 72-73 32.171567644243126 35.0 32.0 38.0 18.0 40.0 74-75 31.798671742110564 35.0 32.0 37.0 16.0 39.5 76-77 29.84148076620164 33.0 29.0 35.5 12.5 38.0 78-79 31.113803020593586 35.0 31.5 36.0 13.0 39.0 80-81 31.125699953314793 35.0 32.0 36.0 11.0 38.5 82-83 30.873905888906528 35.0 32.0 36.0 7.5 37.0 84-85 30.631980864632816 35.0 32.0 35.0 7.0 37.0 86-87 30.38563980079163 35.0 31.5 35.0 2.0 36.5 88-89 30.136255042045764 35.0 31.0 35.0 2.0 36.0 90-91 29.8888589518979 34.5 31.0 35.0 2.0 36.0 92-93 29.652328710883197 34.0 31.0 35.0 2.0 36.0 94-95 29.399851505650545 34.0 31.0 35.0 2.0 35.0 96-97 29.162152278412613 34.0 31.0 35.0 2.0 35.0 98-99 28.884622822991297 34.0 30.5 35.0 2.0 35.0 100-101 28.29446642321397 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3175966959709058 1101 2 0.34313539313574637 1101 3 0.30545889059149545 1101 4 0.2617691088490872 1101 5 0.23762583875604548 1101 6 0.27243844572863907 1101 7 0.2887223692860559 1101 8 0.21553972283275868 1101 9 0.3590593299292095 1101 10-11 0.3454880156533804 1101 12-13 0.3151492409601673 1101 14-15 0.34483027250901443 1101 16-17 0.3178344773018793 1101 18-19 0.2917540104855689 1101 20-21 0.2811244485504929 1101 22-23 0.26858899787823987 1101 24-25 0.2118253004544215 1101 26-27 0.17935412588798272 1101 28-29 0.24337831267471444 1101 30-31 0.18407604319011028 1101 32-33 0.21244582205298457 1101 34-35 0.1489611771211301 1101 36-37 0.1295859818080416 1101 38-39 0.2790719659399059 1101 40-41 0.2836809529561819 1101 42-43 0.3026788687094282 1101 44-45 0.28813162148194493 1101 46-47 0.2691713087228891 1101 48-49 0.16744905738048033 1101 50-51 0.26671389766594444 1101 52-53 0.21481467126126574 1101 54-55 0.1284527684379455 1101 56-57 0.1407825526808324 1101 58-59 0.08777673263487173 1101 60-61 0.127748912225492 1101 62-63 0.05161190690586892 1101 64-65 -0.009319947237479198 1101 66-67 0.005346232459174871 1101 68-69 -0.07162733871677318 1101 70-71 -0.05438448558184916 1101 72-73 -0.08491681583642219 1101 74-75 -0.13659499032899092 1101 76-77 -0.11413921131100224 1101 78-79 -0.07132504886160262 1101 80-81 -0.11664772794892286 1101 82-83 -0.14171821006276986 1101 84-85 -0.1684610033972156 1101 86-87 -0.13166613675529604 1101 88-89 -0.2529101614566329 1101 90-91 -0.1865344217094851 1101 92-93 -0.27430642082484624 1101 94-95 -0.25328784206114463 1101 96-97 -0.39173575270724825 1101 98-99 -0.564510001658661 1101 100-101 -0.6412276867190414 1102 1 0.36819342640067276 1102 2 0.33846435973660505 1102 3 0.3543282524171474 1102 4 0.33880760458949766 1102 5 0.3033255885202877 1102 6 0.3092981588610684 1102 7 0.3175874022679679 1102 8 0.3041927922109835 1102 9 0.39594337579181627 1102 10-11 0.38554414768855594 1102 12-13 0.25554465293030404 1102 14-15 0.3722266380499235 1102 16-17 0.37825535185763925 1102 18-19 0.33535837356529186 1102 20-21 0.3990384001892764 1102 22-23 0.3375867089000195 1102 24-25 0.31111344898548765 1102 26-27 0.26656101433075463 1102 28-29 0.24798746777086222 1102 30-31 0.2707000181100412 1102 32-33 0.2954439819540795 1102 34-35 0.31551305739456126 1102 36-37 0.3648247352371712 1102 38-39 0.4302346280290692 1102 40-41 0.4893108320514443 1102 42-43 0.53700406424759 1102 44-45 0.5180858078856332 1102 46-47 0.5445586075141762 1102 48-49 0.3155697538478819 1102 50-51 0.3954490227957308 1102 52-53 0.4213592738363161 1102 54-55 0.4260982960310926 1102 56-57 0.3534414343485537 1102 58-59 0.41675774595865533 1102 60-61 0.5148156747710573 1102 62-63 0.3993750823986275 1102 64-65 0.3696644607574129 1102 66-67 0.368576626999932 1102 68-69 0.36776063016047544 1102 70-71 0.3923835493102672 1102 72-73 0.31121593060734654 1102 74-75 0.31280828774918845 1102 76-77 0.26503262676982686 1102 78-79 0.3710825948146592 1102 80-81 0.437971415477719 1102 82-83 0.40435130531432506 1102 84-85 0.4396377800003073 1102 86-87 0.3201817299108747 1102 88-89 0.24095596564733768 1102 90-91 0.15680793912346402 1102 92-93 0.19565474720065623 1102 94-95 0.14459090539200403 1102 96-97 0.1814596350322546 1102 98-99 0.10523901218513032 1102 100-101 6.60793485856459E-4 1103 1 0.35555209537606913 1103 2 0.3514233755944218 1103 3 0.34528051547086847 1103 4 0.25176560510549706 1103 5 0.23112228897180387 1103 6 0.2587944061316847 1103 7 0.21297673508686188 1103 8 0.24143060947601214 1103 9 0.29787424351074065 1103 10-11 0.28666606134233064 1103 12-13 0.26086244059941777 1103 14-15 0.3545703971212788 1103 16-17 0.41660416134332223 1103 18-19 0.3445345252167371 1103 20-21 0.27936080896537163 1103 22-23 0.25937974526929963 1103 24-25 0.24299921079515485 1103 26-27 0.20110039800785984 1103 28-29 0.27594653179232864 1103 30-31 0.26602947830486556 1103 32-33 0.3427846845862348 1103 34-35 0.2903756673280995 1103 36-37 0.26476611877316714 1103 38-39 0.3836570914513757 1103 40-41 0.5327021153679965 1103 42-43 0.5148611840456923 1103 44-45 0.5462139002848616 1103 46-47 0.6310859068012746 1103 48-49 0.5454150561086237 1103 50-51 0.5888307970456736 1103 52-53 0.4820356125449763 1103 54-55 0.48674570400662276 1103 56-57 0.4901322492280471 1103 58-59 0.4777381665236433 1103 60-61 0.43259560516025886 1103 62-63 0.45492872876444324 1103 64-65 0.4005127905591692 1103 66-67 0.35461196714334875 1103 68-69 0.3731889989669881 1103 70-71 0.3029893738316076 1103 72-73 0.38313298186726286 1103 74-75 0.3376828279490489 1103 76-77 0.3208366264876119 1103 78-79 0.46959193634987173 1103 80-81 0.3874759937581018 1103 82-83 0.3964884267057265 1103 84-85 0.4248166207455064 1103 86-87 0.444415211926696 1103 88-89 0.3926424028415454 1103 90-91 0.39249210767957976 1103 92-93 0.38557667913057614 1103 94-95 0.3884760418552453 1103 96-97 0.40794682441250885 1103 98-99 0.3279124215024787 1103 100-101 0.22361088344774416 1104 1 0.3148543487468629 1104 2 0.32768363859646854 1104 3 0.2826509982006087 1104 4 0.23770917521265744 1104 5 0.2550104666550155 1104 6 0.2735144308403292 1104 7 0.2767315499830687 1104 8 0.279735435227245 1104 9 0.36622504817015056 1104 10-11 0.2939674420022911 1104 12-13 0.2942245875846581 1104 14-15 0.3567067389876897 1104 16-17 0.35260873629474787 1104 18-19 0.3153736298352001 1104 20-21 0.3328031943986218 1104 22-23 0.3075097126459383 1104 24-25 0.30887325806576627 1104 26-27 0.31846116824930704 1104 28-29 0.25698577691139946 1104 30-31 0.28145702803423234 1104 32-33 0.37569707372148287 1104 34-35 0.290084821806154 1104 36-37 0.342895996166547 1104 38-39 0.399734738508414 1104 40-41 0.4253211692333636 1104 42-43 0.36667994522468206 1104 44-45 0.4130462565394666 1104 46-47 0.5126685303758549 1104 48-49 0.47526748547272035 1104 50-51 0.5593071645915231 1104 52-53 0.43146722571533047 1104 54-55 0.45633694049948303 1104 56-57 0.49067934107699074 1104 58-59 0.48533742660782053 1104 60-61 0.46264052264625377 1104 62-63 0.5231094840838821 1104 64-65 0.5194410595842598 1104 66-67 0.5496896898623547 1104 68-69 0.46690624141875503 1104 70-71 0.498427599883982 1104 72-73 0.43377080471862683 1104 74-75 0.43052754967710527 1104 76-77 0.3975017571021091 1104 78-79 0.46647606022411736 1104 80-81 0.43066318731600717 1104 82-83 0.4334972876482688 1104 84-85 0.4218686077468554 1104 86-87 0.40664052695363395 1104 88-89 0.332439059063816 1104 90-91 0.3491238703488726 1104 92-93 0.2513286660178622 1104 94-95 0.28386786316320567 1104 96-97 0.17763878828689528 1104 98-99 0.13282032997861037 1104 100-101 0.14814256179892737 1105 1 0.266530024576042 1105 2 0.2791420722090905 1105 3 0.23382048546638146 1105 4 0.16563658507488555 1105 5 0.20234200809503733 1105 6 0.21419097705825152 1105 7 0.18384843339499213 1105 8 0.2444456440495486 1105 9 0.2676723356951882 1105 10-11 0.22316777766281604 1105 12-13 0.19935398869188248 1105 14-15 0.24682051427404872 1105 16-17 0.2282414719990058 1105 18-19 0.2719521643163674 1105 20-21 0.3063237820033322 1105 22-23 0.1438148627063498 1105 24-25 0.17102384241892565 1105 26-27 0.20923944913923265 1105 28-29 0.243182731828675 1105 30-31 0.22888689642521598 1105 32-33 0.24701123294440208 1105 34-35 0.2082608249185327 1105 36-37 0.17443246659107814 1105 38-39 0.2451853599205407 1105 40-41 0.2684744187083652 1105 42-43 0.35528253821642153 1105 44-45 0.3769911480106529 1105 46-47 0.38219718576011985 1105 48-49 0.33251982579614037 1105 50-51 0.2322074759720394 1105 52-53 0.2604926316955982 1105 54-55 0.2795979733513292 1105 56-57 0.2336147093877443 1105 58-59 0.25432269247395567 1105 60-61 0.3054838824888151 1105 62-63 0.32621491187355645 1105 64-65 0.3203044909037729 1105 66-67 0.37140517923239 1105 68-69 0.31028809218044984 1105 70-71 0.23103792840250037 1105 72-73 0.3307735010778998 1105 74-75 0.27143205823403704 1105 76-77 0.2729332242822018 1105 78-79 0.339458582874812 1105 80-81 0.3081889830543716 1105 82-83 0.393306767644237 1105 84-85 0.3862149713535281 1105 86-87 0.3567770487779569 1105 88-89 0.3901452652337838 1105 90-91 0.36417676811866073 1105 92-93 0.37202588574345796 1105 94-95 0.3654657435607156 1105 96-97 0.37638595384392204 1105 98-99 0.3353713641509515 1105 100-101 0.4298059698323442 1106 1 0.2840375643902746 1106 2 0.257234480113226 1106 3 0.22557596561352966 1106 4 0.20986158998398707 1106 5 0.18834247449053976 1106 6 0.22688254599251678 1106 7 0.20832757580872396 1106 8 0.21435183153857196 1106 9 0.29253693809980064 1106 10-11 0.2728823691158766 1106 12-13 0.19730553184997035 1106 14-15 0.24420586302478853 1106 16-17 0.2838591663988339 1106 18-19 0.3067010929987575 1106 20-21 0.30799458598983165 1106 22-23 0.29546783809037436 1106 24-25 0.3270346314797976 1106 26-27 0.28465928824386566 1106 28-29 0.30105962953234666 1106 30-31 0.37139840910128896 1106 32-33 0.3730405429328272 1106 34-35 0.3505714462938343 1106 36-37 0.3146963557776772 1106 38-39 0.3610152248530909 1106 40-41 0.38608605058261247 1106 42-43 0.4068450339817815 1106 44-45 0.35649610848283686 1106 46-47 0.40633433868290325 1106 48-49 0.343196322246925 1106 50-51 0.3747811531568672 1106 52-53 0.3051702964757368 1106 54-55 0.33336397536128715 1106 56-57 0.3140152699493157 1106 58-59 0.37773091456099905 1106 60-61 0.457681863683689 1106 62-63 0.4750208697865972 1106 64-65 0.4209344943998943 1106 66-67 0.5420051793063081 1106 68-69 0.5260624263596014 1106 70-71 0.4748708149274563 1106 72-73 0.472892331777814 1106 74-75 0.43827674914784254 1106 76-77 0.37412846746692807 1106 78-79 0.3385882027587819 1106 80-81 0.43329654289922104 1106 82-83 0.3966365956199347 1106 84-85 0.3587470049389303 1106 86-87 0.4140651160009945 1106 88-89 0.4325731507079382 1106 90-91 0.4164318353345564 1106 92-93 0.4109739754066979 1106 94-95 0.5577156694622225 1106 96-97 0.5447282288881645 1106 98-99 0.6095849099714172 1106 100-101 0.6103992111135454 1107 1 0.2532808502654831 1107 2 0.24422000005668565 1107 3 0.2088863875529796 1107 4 0.19881590391782566 1107 5 0.1834327560277984 1107 6 0.1877515942683914 1107 7 0.19171937232218283 1107 8 0.21959044449041443 1107 9 0.3183128073494643 1107 10-11 0.23480731318891657 1107 12-13 0.24846328215924274 1107 14-15 0.215049597447738 1107 16-17 0.26370086864707787 1107 18-19 0.20507424500794968 1107 20-21 0.22462649877702745 1107 22-23 0.19818658301470293 1107 24-25 0.21392320649556495 1107 26-27 0.23771425479494468 1107 28-29 0.3036286112785831 1107 30-31 0.29526807858258763 1107 32-33 0.3270091226651388 1107 34-35 0.31969793589690454 1107 36-37 0.3592060283143539 1107 38-39 0.4083679280857595 1107 40-41 0.39506070681935057 1107 42-43 0.4538959452399709 1107 44-45 0.4079825149518541 1107 46-47 0.40578976358897023 1107 48-49 0.4159898632596537 1107 50-51 0.4533755483777 1107 52-53 0.4338695253254059 1107 54-55 0.4539043120658306 1107 56-57 0.5129435565317593 1107 58-59 0.538429588639552 1107 60-61 0.5642762920322966 1107 62-63 0.5290026663703671 1107 64-65 0.5309887274328418 1107 66-67 0.5170845772363464 1107 68-69 0.5259424945670759 1107 70-71 0.5107636603879229 1107 72-73 0.5193534523976027 1107 74-75 0.4989716725184046 1107 76-77 0.4851996741367195 1107 78-79 0.5255106860813896 1107 80-81 0.5707478879644725 1107 82-83 0.6517137032050151 1107 84-85 0.5796532635746452 1107 86-87 0.5927902616768286 1107 88-89 0.55063706682364 1107 90-91 0.630118569470671 1107 92-93 0.654107587958741 1107 94-95 0.6853300976344663 1107 96-97 0.7187564246194711 1107 98-99 0.7842631713207453 1107 100-101 0.8339516850972188 1108 1 0.13065771745847599 1108 2 0.1355542727857184 1108 3 0.10983886155245415 1108 4 0.12343978668647537 1108 5 0.1167418893356782 1108 6 0.10201455778916113 1108 7 0.13674903159599694 1108 8 0.10642537672530494 1108 9 0.24718313946912218 1108 10-11 0.17210391753356902 1108 12-13 0.09024437402349861 1108 14-15 0.09712582306812578 1108 16-17 0.13611905284296455 1108 18-19 0.10326296326799422 1108 20-21 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-0.6962353744560659 2316 62-63 -1.1806444292780114 2316 64-65 -1.4205170661641517 2316 66-67 -1.0313387211734124 2316 68-69 -1.0240828057167164 2316 70-71 -0.9110245408230497 2316 72-73 -0.9434767415053855 2316 74-75 -0.6855840867535257 2316 76-77 -0.7016885764249245 2316 78-79 -0.7653841214536747 2316 80-81 -0.6615111834472387 2316 82-83 -0.7560437871573562 2316 84-85 -0.8663595931358365 2316 86-87 -1.0395463972718524 2316 88-89 -0.6837593308997789 2316 90-91 -0.6189679726867929 2316 92-93 -0.6523796601606371 2316 94-95 -0.8866304344604039 2316 96-97 -0.8861209929855995 2316 98-99 -0.8089737637874173 2316 100-101 -1.054742878049872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 39.0 4 71.0 5 202.0 6 520.0 7 1235.0 8 3112.0 9 6210.0 10 10945.0 11 17678.0 12 27464.0 13 40112.0 14 47430.0 15 62095.0 16 83964.0 17 115251.0 18 95295.0 19 49485.0 20 35503.0 21 33663.0 22 34180.0 23 37245.0 24 43083.0 25 51964.0 26 64169.0 27 79663.0 28 100736.0 29 126578.0 30 158682.0 31 200318.0 32 256540.0 33 338261.0 34 474869.0 35 730517.0 36 1119417.0 37 1710860.0 38 1613328.0 39 310939.0 40 159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.47276078147753 21.674385680133376 15.16935632328766 21.683497215101426 2 15.361476493856685 22.00914369770858 39.21391124960899 23.41546855882574 3 17.38211189770959 28.378905423487467 30.704050868039616 23.53493181076333 4 11.66388716140788 18.18610616926432 38.59130433491459 31.558702334413212 5 11.546195659235067 39.01574334990065 34.7830514636325 14.65500952723177 6 28.350295670551606 39.13133860372227 18.167980964236175 14.350384761489948 7 26.04529764387563 33.914669140706344 22.41909557401214 17.62093764140588 8 26.66727626766796 33.5033369468072 20.34191934483813 19.48746744068671 9 26.480004840517207 15.645883005243522 22.154423635087365 35.7196885191519 10-11 23.87533227490405 27.353195524702645 28.29116877245094 20.48030342794236 12-13 25.105661945991287 25.710825023446233 28.727972774838147 20.455540255724337 14-15 22.195304898955584 26.392437868910207 28.332365926508672 23.079891305625534 16-17 19.86119038742536 29.74912485342045 28.60243567358663 21.787249085567563 18-19 19.91451389770931 27.237756922777486 33.01283881530328 19.83489036420992 20-21 21.503694469811983 26.268867916648908 32.81315048247955 19.414287131059563 22-23 20.76675102505151 26.32934366388432 33.18777562740131 19.71612968366286 24-25 20.126428227487413 26.753324578204978 33.096136048433635 20.02411114587398 26-27 19.717608875807226 26.935271889593665 33.00302566338205 20.34409357121706 28-29 20.148343150465795 26.711777978889383 33.23721719144154 19.90266167920328 30-31 20.35343599874754 27.07675789820928 32.828426531358325 19.741379571684856 32-33 19.65705393162672 26.836984144082315 33.211827662865915 20.294134261425054 34-35 20.20133991910443 26.721029327536478 33.00908737168224 20.06854338167685 36-37 20.67686485019656 26.86177833029436 32.332787615621115 20.12856920388796 38-39 20.393678797034685 27.08476070779873 32.53636703692096 19.98519345824564 40-41 20.037042034654025 27.038334286420827 32.627572199393704 20.297051479531447 42-43 20.116866884068006 27.059816262065027 32.26072545494445 20.562591398922514 44-45 20.39277872078316 27.341319168702327 31.661460683027364 20.604441427487146 46-47 20.777556937242174 27.693269117860503 30.963644131981827 20.565529812915496 48-49 20.93525385491827 27.582104784879867 30.617330130676706 20.865311229525155 50-51 20.967165661728725 27.86090237588698 29.9073032680781 21.264628694306186 52-53 20.879816121036615 28.040459212357227 29.90459029844324 21.175134368162922 54-55 21.02676816477468 28.56919690556239 29.67840908261316 20.72562584704977 56-57 21.033504716941735 28.14352297098192 29.6334745874126 21.18949772466374 58-59 21.060062569520227 27.720226503469767 29.837341148656222 21.382369778353784 60-61 21.26465117910743 28.100769881987482 29.570637954631945 21.063940984273145 62-63 20.753787659902347 28.056592448029612 29.82452178554843 21.36509810651961 64-65 20.821228054513025 28.097721748322428 29.75035640700167 21.330693790162876 66-67 20.77019857121777 27.9955316913031 29.60596657182905 21.628303165650077 68-69 21.221844898011714 28.480784905854634 29.203743070467613 21.09362712566604 70-71 21.392658225091406 28.105701503219414 29.171178981160633 21.330461290528543 72-73 21.041908905046842 28.136498525132513 29.33524983410138 21.486342735719262 74-75 20.952938427275054 28.36576240685758 29.2647158633812 21.416583302486163 76-77 21.043144180796595 28.621883357877664 28.92408088982495 21.41089157150079 78-79 21.103392414636527 28.566886509589338 29.0737661158457 21.255954959928435 80-81 20.90829584743617 28.54749974819673 29.065199847655926 21.47900455671117 82-83 20.750311193821165 28.50745132879143 29.3785774915922 21.36365998579521 84-85 20.706206744314127 28.49955226231576 29.118936130222433 21.67530486314768 86-87 20.768858832187647 28.78951863803175 28.860583123514914 21.58103940626569 88-89 20.613546511343742 28.556896390757665 29.156303325411397 21.673253772487193 90-91 20.39467611353566 28.48654798686908 29.15262773009111 21.96614816950415 92-93 20.524952261459646 28.81264127066028 28.996765255684622 21.665641212195453 94-95 20.443819827096814 28.379664894277823 29.16720491656013 22.009310362065236 96-97 20.498537015481887 28.807487273513328 28.743547048560046 21.950428662444736 98-99 20.046058159392313 28.594757180030015 28.978882452086268 22.380302208491404 100-101 20.071876421405758 28.875553281031372 28.702404715392188 22.35016558217068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15136.0 1 10408.0 2 11113.0 3 14013.5 4 8581.5 5 5948.0 6 9338.0 7 18226.0 8 26160.5 9 26640.5 10 20265.0 11 14417.5 12 13426.0 13 13930.5 14 14693.0 15 15532.0 16 16486.0 17 17489.0 18 18617.0 19 20033.5 20 21763.0 21 24115.5 22 28408.5 23 35438.0 24 42420.0 25 49243.0 26 59312.0 27 73618.5 28 94756.5 29 112235.5 30 122995.5 31 139530.5 32 161617.0 33 181331.0 34 200244.5 35 224981.0 36 245700.0 37 261122.5 38 278624.5 39 289938.0 40 297329.0 41 299225.0 42 294415.5 43 295432.5 44 297032.0 45 294861.5 46 291613.5 47 283248.0 48 269817.5 49 267125.5 50 263404.5 51 243330.5 52 218079.0 53 194640.5 54 177749.0 55 168154.0 56 153872.5 57 135605.0 58 121483.0 59 107866.5 60 92690.5 61 76638.0 62 63030.5 63 51207.0 64 41607.0 65 33438.0 66 26206.5 67 19985.0 68 14683.0 69 10673.5 70 7656.5 71 5313.5 72 3783.0 73 2751.5 74 2129.5 75 1665.0 76 1131.0 77 828.5 78 620.5 79 389.0 80 238.0 81 160.5 82 121.5 83 85.5 84 55.5 85 35.0 86 23.5 87 18.5 88 16.5 89 12.5 90 8.0 91 5.5 92 4.5 93 4.5 94 2.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05092931775377952 2 0.001942639185457576 3 1.856024699481761E-4 4 1.856024699481761E-4 5 0.002697422563246826 6 0.0019673861814506663 7 0.0 8 0.0 9 9.898798397236057E-5 10-11 2.4746995993090144E-5 12-13 6.186748998272536E-6 14-15 5.691809078410733E-4 16-17 0.0 18-19 9.280123497408805E-5 20-21 5.877411548358909E-4 22-23 0.0 24-25 6.12488150828981E-4 26-27 0.007250869825975412 28-29 0.0073436710609495 30-31 0.0012311630506562346 32-33 0.006774490153108426 34-35 0.007789116988825123 36-37 0.004732862983678489 38-39 0.0027964105472191862 40-41 0.001973572930448939 42-43 0.0014291390186009557 44-45 7.795303737823395E-4 46-47 4.949399198618028E-4 48-49 0.002041627169429937 50-51 0.0013920185246113206 52-53 0.0020849344124178448 54-55 7.671568757857944E-4 56-57 2.041627169429937E-4 58-59 7.238496327978867E-4 60-61 0.006007333277322633 62-63 0.0026726755672537355 64-65 0.0010826810746976938 66-67 0.005339164385509199 68-69 0.007622074765871764 70-71 0.0024870730973055592 72-73 5.691809078410733E-4 74-75 0.0034707661880308926 76-77 0.0019117054404662135 78-79 0.002295283878359111 80-81 0.001027000333713241 82-83 1.1136148196890566E-4 84-85 5.939279038341634E-4 86-87 0.004906091955630121 88-89 0.0026912358142485533 90-91 0.001224976301657962 92-93 8.042773697754296E-5 94-95 5.07313417858348E-4 96-97 2.722169559239916E-4 98-99 6.248616488255262E-4 100-101 1.2373497996545072E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8081789.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.686596653804116 #Duplication Level Percentage of deduplicated Percentage of total 1 66.38095406015236 28.335770114604248 2 18.170711790930643 15.512916902639578 3 7.007157312869561 8.973350937126504 4 3.138650217713094 5.359131835235733 5 1.6450968868614295 3.5111793632941333 6 0.9409464666457366 2.4099481376717224 7 0.5709190114542982 1.7059412694756737 8 0.39155242095883475 1.3371232209832238 9 0.2873512171449351 1.1039440953820976 >10 1.2354320857940775 9.462751323183376 >50 0.11449860627797662 3.431600959340269 >100 0.09957600785287248 8.678303964565188 >500 0.011075936810689956 3.166200372210209 >1k 0.005756546980947649 4.14086838236196 >5k 1.1688420092697408E-4 0.3336404250552976 >10k+ 2.0454735162220465E-4 2.537328696870739 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63865 0.7902334495493509 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46919 0.5805521524998982 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37745 0.46703768187959377 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17510 0.2166599499195042 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15075 0.18653048229791697 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11161 0.13810061113943953 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11143 0.13787788817550173 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9608 0.11888456875080505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.712049398963522E-5 0.0 3 0.0 1.2373497996545072E-5 0.0 3.712049398963522E-5 0.0 4 0.0 1.2373497996545072E-5 0.0 3.712049398963522E-5 0.0 5 1.2373497996545072E-5 4.949399198618029E-5 0.0 4.949399198618029E-5 0.0 6 1.2373497996545072E-5 4.949399198618029E-5 0.0 4.949399198618029E-5 0.0 7 1.2373497996545072E-5 6.186748998272536E-5 0.0 4.949399198618029E-5 0.0 8 1.2373497996545072E-5 6.186748998272536E-5 0.0 4.949399198618029E-5 0.0 9 1.2373497996545072E-5 6.186748998272536E-5 0.0 6.186748998272536E-5 0.0 10-11 1.2373497996545072E-5 6.186748998272536E-5 0.0 1.1136148196890564E-4 0.0 12-13 1.2373497996545072E-5 6.186748998272536E-5 0.0 1.73228971951631E-4 0.0 14-15 1.2373497996545072E-5 6.80542389809979E-5 0.0 1.7941572094990355E-4 0.0 16-17 1.2373497996545072E-5 8.042773697754298E-5 0.0 1.917892189464486E-4 0.0 18-19 1.2373497996545072E-5 8.66144859758155E-5 0.0 2.1034946594126623E-4 0.0 20-21 1.2373497996545072E-5 8.66144859758155E-5 0.0 2.722169559239916E-4 0.0 22-23 1.2373497996545072E-5 8.66144859758155E-5 0.0 5.011266688600754E-4 0.0 24-25 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.0011631088116752367 0.0 26-27 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.0016704222295335848 0.0 28-29 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.0031181214951293583 0.0 30-31 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.006922972129066968 0.0 32-33 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.01407485397107002 0.0 34-35 1.2373497996545072E-5 8.66144859758155E-5 0.0 0.024202562081242163 0.0 36-37 1.2373497996545072E-5 9.898798397236057E-5 0.0 0.039861223795869954 0.0 38-39 1.2373497996545072E-5 1.237349799654507E-4 0.0 0.06420608110407239 0.0 40-41 1.2373497996545072E-5 1.4848197595854087E-4 0.0 0.09410045226372526 0.0 42-43 1.2373497996545072E-5 1.4848197595854087E-4 0.0 0.12203981073992405 0.0 44-45 1.2373497996545072E-5 1.546687249568134E-4 0.0 0.15528121310764237 0.0 46-47 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.19642309394615476 0.0 48-49 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.24215554254138533 0.0 50-51 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.29184751049551033 6.186748998272536E-6 52-53 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.34003609844305516 1.2373497996545072E-5 54-55 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.39222751299248226 1.2373497996545072E-5 56-57 1.2373497996545072E-5 1.6085547395508593E-4 0.0 0.4540145752382301 1.2373497996545072E-5 58-59 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.5259417190921465 1.2373497996545072E-5 60-61 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.60479183507513 1.2373497996545072E-5 62-63 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.6870384762581652 1.2373497996545072E-5 64-65 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.7727435101312345 1.2373497996545072E-5 66-67 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.8699744573880857 1.2373497996545072E-5 68-69 1.2373497996545072E-5 1.8560246994817607E-4 0.0 0.9841447234022072 1.2373497996545072E-5 70-71 1.2373497996545072E-5 1.8560246994817607E-4 0.0 1.1151181996956367 1.2373497996545072E-5 72-73 1.2373497996545072E-5 2.0416271694299366E-4 0.0 1.255613082697408 1.2373497996545072E-5 74-75 1.2373497996545072E-5 2.2272296393781128E-4 0.0 1.3991704064533237 1.2373497996545072E-5 76-77 1.2373497996545072E-5 2.2272296393781128E-4 0.0 1.5484071657896537 1.2373497996545072E-5 78-79 1.2373497996545072E-5 2.3509646193435637E-4 0.0 1.7255523498571912 1.2373497996545072E-5 80-81 1.2373497996545072E-5 2.3509646193435637E-4 0.0 1.9269124695039674 1.2373497996545072E-5 82-83 1.2373497996545072E-5 2.3509646193435637E-4 0.0 2.1443210160522628 1.2373497996545072E-5 84-85 1.2373497996545072E-5 2.3509646193435637E-4 0.0 2.3625895207113174 1.2373497996545072E-5 86-87 1.2373497996545072E-5 2.3509646193435637E-4 0.0 2.592049854308248 1.2373497996545072E-5 88-89 1.2373497996545072E-5 2.3509646193435637E-4 0.0 2.850934366140962 1.2373497996545072E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23445 0.0 57.24249 1 GTATCAA 46015 0.0 46.309517 1 TATAACG 945 0.0 44.232063 2 TACCTGG 7150 0.0 39.79304 2 GTACCTG 7860 0.0 36.156193 1 ATCAACG 64265 0.0 33.14847 3 TATCAAC 64930 0.0 32.729317 2 ATAACGC 1485 0.0 32.62491 3 TCAACGC 65360 0.0 32.563858 4 GTGGTAT 8950 0.0 31.752813 1 CAACGCA 67350 0.0 31.644 5 AACGCAG 71190 0.0 30.170261 6 TGGTATC 9415 0.0 29.967634 2 ACCTGGG 9460 0.0 29.523087 3 CGCAGAG 80495 0.0 26.493189 8 GTATAAC 2075 0.0 26.33806 1 ACGCAGA 81590 0.0 26.22495 7 GTACATG 49925 0.0 26.100777 1 TACATGG 48620 0.0 25.596033 2 GTACACG 710 0.0 25.434856 1 >>END_MODULE