FastQCFastQC Report
Wed 25 May 2016
SRR2049417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566793
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399030.3449789410081083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338510.29265674591046975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187330.16195500343094235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256100.049.4680021
GTATCAA455300.039.1345521
ATCAACG542300.032.479073
TCAACGC548750.032.0980034
TATCAAC564300.031.3204962
CAACGCA570150.030.8761735
AACGCAG598850.029.499676
CGCAGAG656300.026.4705858
ACGCAGA661800.026.465947
GCAGAGT704050.023.9465779
GTACATG355100.023.3697011
GTGGTAT130900.022.7530081
TACATGG349450.022.4001642
GAGTACT462550.022.17353412-13
TGGTATC131150.021.6214622
ACATGGG352300.021.34383
AGAGTAC615900.021.16446910-11
CAGAGTA685550.020.70844810-11
GTACTTT515750.019.7736514-15
AGTACTT488650.019.61842712-13
Produced by FastQC (version 0.11.5)