##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049417_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11566793 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.513117162207365 31.0 31.0 34.0 30.0 34.0 2 31.55114939810888 33.0 31.0 34.0 30.0 34.0 3 31.57664669887323 33.0 31.0 34.0 28.0 34.0 4 35.1953062529951 37.0 35.0 37.0 33.0 37.0 5 35.22251353508271 37.0 35.0 37.0 33.0 37.0 6 35.311040233883325 37.0 35.0 37.0 33.0 37.0 7 35.25567294236181 37.0 35.0 37.0 33.0 37.0 8 35.231192085827075 37.0 35.0 37.0 33.0 37.0 9 36.82709260898851 39.0 37.0 39.0 33.0 39.0 10-11 36.769006456673 39.0 37.0 39.0 33.0 39.0 12-13 36.730520162330215 39.0 37.0 39.0 33.0 39.0 14-15 37.91527072370017 40.0 38.0 41.0 33.0 41.0 16-17 37.91036603663608 40.0 38.0 41.0 33.0 41.0 18-19 37.945745462895374 40.0 38.0 41.0 33.0 41.0 20-21 37.91676694655122 40.0 38.0 41.0 33.0 41.0 22-23 37.81518503011163 40.0 38.0 41.0 33.0 41.0 24-25 37.71381280878805 40.0 38.0 41.0 32.0 41.0 26-27 37.57383922233241 40.0 37.5 41.0 32.0 41.0 28-29 37.42335057781358 40.0 37.0 41.0 32.0 41.0 30-31 37.24185562930019 40.0 37.0 41.0 32.0 41.0 32-33 36.99614430724229 40.0 36.5 41.0 31.0 41.0 34-35 36.79586359849269 40.0 36.0 41.0 30.5 41.0 36-37 36.60308116519419 39.5 36.0 41.0 30.0 41.0 38-39 36.397375054606755 39.0 36.0 41.0 30.0 41.0 40-41 36.18590822884096 39.0 35.0 41.0 30.0 41.0 42-43 35.96074564488187 39.0 35.0 40.5 29.5 41.0 44-45 35.69052705447396 39.0 35.0 40.0 28.5 41.0 46-47 35.34444750589036 39.0 35.0 40.0 26.5 41.0 48-49 35.06352616494477 39.0 35.0 40.0 26.0 41.0 50-51 33.71356866159877 37.5 33.0 39.0 23.5 40.0 52-53 33.99927603096208 37.5 33.5 39.5 24.5 40.0 54-55 35.05173901702918 39.0 35.0 40.0 26.0 41.0 56-57 35.18483641057638 39.0 35.0 41.0 26.0 41.0 58-59 35.04933212689117 39.0 35.0 41.0 26.0 41.0 60-61 34.8118942303195 39.0 34.5 40.0 26.0 41.0 62-63 34.56468508600439 38.0 34.0 40.0 25.0 41.0 64-65 34.30592036184965 38.0 34.0 40.0 24.5 41.0 66-67 34.006499078871734 37.5 34.0 40.0 23.0 41.0 68-69 33.6786657286942 37.0 34.0 40.0 22.5 41.0 70-71 33.35097265075981 36.5 33.0 39.5 22.0 41.0 72-73 33.09817332254498 36.0 33.0 39.0 22.0 41.0 74-75 32.9000640021828 36.0 33.0 39.0 21.5 40.5 76-77 32.54944689509011 35.0 33.0 38.5 20.0 40.0 78-79 32.16619866025094 35.0 33.0 37.0 19.0 39.5 80-81 31.806548971698554 35.0 32.5 37.0 18.0 39.0 82-83 31.442510858454888 35.0 32.0 36.5 16.0 39.0 84-85 31.10405291250565 35.0 32.0 36.0 11.5 37.5 86-87 30.78885028892624 35.0 32.0 36.0 7.0 37.0 88-89 30.481206458868936 35.0 31.5 35.0 4.0 37.0 90-91 30.199709720749738 35.0 31.0 35.0 2.0 36.0 92-93 29.939395301705495 34.5 31.0 35.0 2.0 36.0 94-95 29.676130929290427 34.0 31.0 35.0 2.0 36.0 96-97 29.419467651923917 34.0 31.0 35.0 2.0 36.0 98-99 29.11259616213414 34.0 31.0 35.0 2.0 35.0 100-101 28.58847292417181 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23692986617365008 1101 2 0.27600854043196676 1101 3 0.25205820652938726 1101 4 0.2462571934005382 1101 5 0.26177277525890474 1101 6 0.26565212700410257 1101 7 0.2776446280120126 1101 8 0.26548726715012094 1101 9 0.3003385127327789 1101 10-11 0.3053595086329821 1101 12-13 0.3126289489772489 1101 14-15 0.38831995995500534 1101 16-17 0.3215272186112017 1101 18-19 0.32654879060960695 1101 20-21 0.28441471722629075 1101 22-23 0.310959932402298 1101 24-25 0.2955911154282802 1101 26-27 0.28210584285025675 1101 28-29 0.3139300122363835 1101 30-31 0.3027358236540607 1101 32-33 0.2903587164413821 1101 34-35 0.270139163242618 1101 36-37 0.29894370165072104 1101 38-39 0.21154064263881622 1101 40-41 0.17538047437690096 1101 42-43 0.11099565077856965 1101 44-45 0.07054575351597236 1101 46-47 0.022606686347593552 1101 48-49 -0.02844083249660656 1101 50-51 -0.03224634346921107 1101 52-53 0.11402195314815344 1101 54-55 0.3054145513286528 1101 56-57 0.2825596027587096 1101 58-59 0.2677337065031793 1101 60-61 0.26598903375474237 1101 62-63 0.2243556160442921 1101 64-65 0.23541083974892274 1101 66-67 0.18733818145516778 1101 68-69 0.15122723122860293 1101 70-71 0.06217976084602839 1101 72-73 0.08796090079206209 1101 74-75 0.05244905392183341 1101 76-77 0.014835885536804483 1101 78-79 -0.03650521154931141 1101 80-81 -0.11561422596633619 1101 82-83 -0.09371235658028354 1101 84-85 -0.1556766651999233 1101 86-87 -0.25024984989786603 1101 88-89 -0.18815591277094157 1101 90-91 -0.2011941354379907 1101 92-93 -0.2680659602187845 1101 94-95 -0.4306201405861252 1101 96-97 -0.4565904872661086 1101 98-99 -0.5112521312697424 1101 100-101 -0.6702424373013791 1102 1 0.3349204251093063 1102 2 0.3529860765698345 1102 3 0.36646497200038297 1102 4 0.3388799920451291 1102 5 0.33884478072473456 1102 6 0.34575701411102244 1102 7 0.32659519242319135 1102 8 0.3730572934165508 1102 9 0.4100828564694794 1102 10-11 0.40411766673879157 1102 12-13 0.38018808527755255 1102 14-15 0.49665511761161696 1102 16-17 0.4195638600486262 1102 18-19 0.38496132173952446 1102 20-21 0.4013802834751843 1102 22-23 0.3642476898663034 1102 24-25 0.4146974947487223 1102 26-27 0.4030458703911606 1102 28-29 0.4008294749923138 1102 30-31 0.34607332087770715 1102 32-33 0.3675790119613467 1102 34-35 0.3609099428588749 1102 36-37 0.33169740888612154 1102 38-39 0.3088715029934974 1102 40-41 0.30687970978770807 1102 42-43 0.26814557717414544 1102 44-45 0.3298829318976715 1102 46-47 0.262714341195327 1102 48-49 0.13409180439174406 1102 50-51 0.10518158380101283 1102 52-53 0.2784992963287465 1102 54-55 0.4025401195001024 1102 56-57 0.38241311231019637 1102 58-59 0.36966742300180755 1102 60-61 0.4030072736706032 1102 62-63 0.3307567318858915 1102 64-65 0.2967849659714261 1102 66-67 0.2922489894288134 1102 68-69 0.27646895406337535 1102 70-71 0.24817520842589147 1102 72-73 0.23734712763607746 1102 74-75 0.21378407201133598 1102 76-77 0.18526828077248325 1102 78-79 0.14342645813513855 1102 80-81 0.10932899276888364 1102 82-83 0.03717008932183319 1102 84-85 0.03391902218276144 1102 86-87 0.016917076033205802 1102 88-89 0.0697905181127858 1102 90-91 -0.005138581509019247 1102 92-93 -0.06890715105920364 1102 94-95 -0.15945396393524192 1102 96-97 -0.19925479006269597 1102 98-99 -0.2027064476632745 1102 100-101 -0.2553160379019026 1103 1 0.2635963755853581 1103 2 0.2926788295789038 1103 3 0.3020937854635122 1103 4 0.3015753220515691 1103 5 0.31959631235722696 1103 6 0.2909563217426552 1103 7 0.2845639397445012 1103 8 0.30095696524306703 1103 9 0.40071327563349257 1103 10-11 0.4590938973342915 1103 12-13 0.37907710145614715 1103 14-15 0.4060247152700356 1103 16-17 0.486041528250702 1103 18-19 0.41953849097151874 1103 20-21 0.45322999675446596 1103 22-23 0.4367480324703763 1103 24-25 0.45547454738293425 1103 26-27 0.42834803907300056 1103 28-29 0.40881929164174835 1103 30-31 0.42772147881940015 1103 32-33 0.36402840502415046 1103 34-35 0.3690511040233915 1103 36-37 0.3853603085979458 1103 38-39 0.3785461094950904 1103 40-41 0.3536662465099809 1103 42-43 0.3570907200119393 1103 44-45 0.25620671295094155 1103 46-47 0.2254933819042435 1103 48-49 0.23735304107489696 1103 50-51 0.11918641300052002 1103 52-53 0.2790641667438081 1103 54-55 0.382127263744529 1103 56-57 0.4396372973785745 1103 58-59 0.442700275784091 1103 60-61 0.42108482448882256 1103 62-63 0.4027174415422152 1103 64-65 0.3710372498953092 1103 66-67 0.35662428981431304 1103 68-69 0.3203969662390236 1103 70-71 0.2924988114978504 1103 72-73 0.36599824285379867 1103 74-75 0.3358415825141563 1103 76-77 0.26312629949924116 1103 78-79 0.2992702772327931 1103 80-81 0.2782024295427199 1103 82-83 0.28914903403279624 1103 84-85 0.2865947825041921 1103 86-87 0.21198300856293883 1103 88-89 0.17191864947993096 1103 90-91 0.13974975281064772 1103 92-93 0.058066897942484985 1103 94-95 0.09278075434434996 1103 96-97 0.04056996090303144 1103 98-99 0.006843000116777631 1103 100-101 0.021463617987965478 1104 1 0.12153763575390997 1104 2 0.16189506335425463 1104 3 0.2180584774643215 1104 4 0.12708390718805163 1104 5 0.06547272424771222 1104 6 0.08186197553857255 1104 7 0.04523443351447298 1104 8 0.02823051312927305 1104 9 0.08062343108510106 1104 10-11 0.0878906190096913 1104 12-13 0.053593566661263026 1104 14-15 0.09602830693433617 1104 16-17 0.1420815782817968 1104 18-19 0.08480132727514444 1104 20-21 0.1070557348896628 1104 22-23 0.1339549394585191 1104 24-25 0.12652660016362205 1104 26-27 0.06567442065026796 1104 28-29 0.04669611897084991 1104 30-31 0.027296720498306115 1104 32-33 0.05551066578349406 1104 34-35 0.06646636977443166 1104 36-37 0.0949061260519315 1104 38-39 0.06448196140716789 1104 40-41 -0.0033290268050834015 1104 42-43 -0.01964696619485551 1104 44-45 -0.04787086253647033 1104 46-47 0.03358308672392951 1104 48-49 -0.013731633511277153 1104 50-51 -0.1233060985162453 1104 52-53 -0.07790573650134291 1104 54-55 0.07556558514887257 1104 56-57 -0.03423907025556616 1104 58-59 0.03191964378918044 1104 60-61 -0.04791271958133336 1104 62-63 0.028860678458570987 1104 64-65 0.018498892610018913 1104 66-67 0.025286960858558416 1104 68-69 0.01666275665380823 1104 70-71 0.02889644626972654 1104 72-73 -0.07778453747744862 1104 74-75 0.011268990862468797 1104 76-77 -0.034531027353146726 1104 78-79 -0.02498173101792389 1104 80-81 -0.008331311341603254 1104 82-83 -0.05894231136491257 1104 84-85 -0.06886317015912269 1104 86-87 -0.08829991976533691 1104 88-89 -0.0840915290040094 1104 90-91 -0.08571060093795069 1104 92-93 -0.14541362878894404 1104 94-95 -0.10551174180166001 1104 96-97 -0.1448881574241625 1104 98-99 -0.16681828107591912 1104 100-101 -0.15340471194405225 1105 1 0.28429418583338517 1105 2 0.34552521263739067 1105 3 0.3067300516591729 1105 4 0.30459645393450785 1105 5 0.3053825080788002 1105 6 0.3208419052747047 1105 7 0.3508617333737831 1105 8 0.36016915607447686 1105 9 0.45967000584626305 1105 10-11 0.42593804317306905 1105 12-13 0.3922959065657352 1105 14-15 0.4611575782385344 1105 16-17 0.4243619445853355 1105 18-19 0.4475967027726142 1105 20-21 0.4905235109253425 1105 22-23 0.42753560717090977 1105 24-25 0.4685984586677563 1105 26-27 0.4634840088528236 1105 28-29 0.5034047251624543 1105 30-31 0.47828528246453317 1105 32-33 0.4455390669441286 1105 34-35 0.4636392657004649 1105 36-37 0.44302062766645633 1105 38-39 0.4585234598806949 1105 40-41 0.4900377446254467 1105 42-43 0.4458900954240974 1105 44-45 0.42919346611922293 1105 46-47 0.4038460132735153 1105 48-49 0.39057836614952635 1105 50-51 0.35980286984457166 1105 52-53 0.46445219845108454 1105 54-55 0.5788792281376374 1105 56-57 0.5993037777417385 1105 58-59 0.6149149935676022 1105 60-61 0.5774671743119271 1105 62-63 0.5889489118274156 1105 64-65 0.6011510168648329 1105 66-67 0.5857774608720874 1105 68-69 0.5936302417529689 1105 70-71 0.5834164020234454 1105 72-73 0.5894287169609171 1105 74-75 0.5326172895372423 1105 76-77 0.5199806225665142 1105 78-79 0.5428451183631466 1105 80-81 0.5156184631346754 1105 82-83 0.556375993897607 1105 84-85 0.5748424611498955 1105 86-87 0.5334030198726261 1105 88-89 0.5567262785183331 1105 90-91 0.5448346287137156 1105 92-93 0.5570069598867526 1105 94-95 0.5484527053951318 1105 96-97 0.5170042337871266 1105 98-99 0.5527430587863833 1105 100-101 0.5670955175118735 1106 1 0.24806451073112612 1106 2 0.25940129165557124 1106 3 0.25240431878654945 1106 4 0.27690499244215516 1106 5 0.250213531963567 1106 6 0.27033194590529774 1106 7 0.3281190275466912 1106 8 0.31877843149706564 1106 9 0.2849113419379492 1106 10-11 0.36663439934554987 1106 12-13 0.33944852461785047 1106 14-15 0.39861590522843215 1106 16-17 0.39819548906411484 1106 18-19 0.4454962784673313 1106 20-21 0.4238013563152023 1106 22-23 0.5003512941152479 1106 24-25 0.41936205369665913 1106 26-27 0.4507255012391056 1106 28-29 0.4756519212625534 1106 30-31 0.4737137242731748 1106 32-33 0.5021799646063911 1106 34-35 0.4733638531022848 1106 36-37 0.4914254997859899 1106 38-39 0.5093806678194781 1106 40-41 0.46845195375208704 1106 42-43 0.5245678973341015 1106 44-45 0.512686985313799 1106 46-47 0.514919291751319 1106 48-49 0.4814151416868384 1106 50-51 0.4335633973654325 1106 52-53 0.5996865005548528 1106 54-55 0.6667772441048214 1106 56-57 0.6820231311034561 1106 58-59 0.6965051865532388 1106 60-61 0.7146807153625829 1106 62-63 0.6551935370249069 1106 64-65 0.703482151965396 1106 66-67 0.7101540873528762 1106 68-69 0.7459130153522864 1106 70-71 0.774036713798381 1106 72-73 0.7409287083372149 1106 74-75 0.7614656133194728 1106 76-77 0.7111164512093637 1106 78-79 0.7194135423828172 1106 80-81 0.7229332795383421 1106 82-83 0.7361572452557965 1106 84-85 0.7385014298107073 1106 86-87 0.6850018108160789 1106 88-89 0.738814502512529 1106 90-91 0.6542802981533242 1106 92-93 0.6275160179729689 1106 94-95 0.6098634954825606 1106 96-97 0.589916650316944 1106 98-99 0.5653338174255964 1106 100-101 0.568372992440846 1107 1 0.22553406624094663 1107 2 0.2390609055499766 1107 3 0.21934661972733593 1107 4 0.2495093072421355 1107 5 0.241553966380728 1107 6 0.2559941023232213 1107 7 0.25614852486992135 1107 8 0.29851819224506215 1107 9 0.3485909941580232 1107 10-11 0.29332005441929 1107 12-13 0.32163589701505657 1107 14-15 0.4149644137304236 1107 16-17 0.3218079429489151 1107 18-19 0.30917807755388793 1107 20-21 0.36672196790797074 1107 22-23 0.3846109711088559 1107 24-25 0.37643627859300466 1107 26-27 0.41182522808074395 1107 28-29 0.4652183693542611 1107 30-31 0.41328975689332736 1107 32-33 0.4260427943201037 1107 34-35 0.4716700489872494 1107 36-37 0.4478180238594618 1107 38-39 0.5181278476175919 1107 40-41 0.439233164523678 1107 42-43 0.42595215809301834 1107 44-45 0.5192376152140383 1107 46-47 0.48582605461268713 1107 48-49 0.45417652565144806 1107 50-51 0.4637247571516241 1107 52-53 0.5387894800548025 1107 54-55 0.6038312672421 1107 56-57 0.5370023282783478 1107 58-59 0.5434054479448349 1107 60-61 0.5910784202010788 1107 62-63 0.6172930205550173 1107 64-65 0.6195499470673269 1107 66-67 0.666386296505074 1107 68-69 0.6578923945724071 1107 70-71 0.6711171507476621 1107 72-73 0.6574909969387548 1107 74-75 0.6618541409967449 1107 76-77 0.6830209554201048 1107 78-79 0.673090188424105 1107 80-81 0.6619048589294643 1107 82-83 0.6102801908969155 1107 84-85 0.6635190417529664 1107 86-87 0.6435284022041934 1107 88-89 0.6672630418682957 1107 90-91 0.6818841926313546 1107 92-93 0.6749857604496903 1107 94-95 0.6686971906057977 1107 96-97 0.6729884327071609 1107 98-99 0.764421819208259 1107 100-101 0.7597253378656106 1108 1 0.1465935869516386 1108 2 0.14822308209648227 1108 3 0.1423015499776774 1108 4 0.18203988632507873 1108 5 0.20036907489130584 1108 6 0.2030558893083807 1108 7 0.21080972739370907 1108 8 0.200048607837104 1108 9 0.23597323443203067 1108 10-11 0.25804652970047215 1108 12-13 0.23358408939301967 1108 14-15 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-0.901608127211869 2316 90-91 -0.8541434603550861 2316 92-93 -0.9760579634146289 2316 94-95 -1.2659929457891472 2316 96-97 -0.9955793380407449 2316 98-99 -0.8089817146184757 2316 100-101 -0.783621082288402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97173.0 3 20720.0 4 16162.0 5 14609.0 6 15055.0 7 16262.0 8 18766.0 9 22770.0 10 27937.0 11 34073.0 12 42023.0 13 51161.0 14 59286.0 15 69204.0 16 80863.0 17 99652.0 18 81485.0 19 50478.0 20 45751.0 21 46131.0 22 48457.0 23 53614.0 24 61304.0 25 73122.0 26 87741.0 27 106473.0 28 130375.0 29 160301.0 30 200280.0 31 254199.0 32 330103.0 33 442170.0 34 620500.0 35 929372.0 36 1457692.0 37 2305227.0 38 2668358.0 39 727549.0 40 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0741360994338 21.126465846306345 14.995855656842508 21.803542397417345 2 15.317670644675339 21.185896283118755 40.89011171808751 22.606321354118393 3 17.965781185332215 26.629188332648727 31.333758763693954 24.0712717183251 4 12.20512784155508 17.773030769020608 39.95943667855945 30.06240471086486 5 12.306963543235499 38.01547715344044 35.97783696803553 13.699722335288525 6 28.81779721722647 39.21244467485271 18.303635077572146 13.66612303034867 7 25.242058111237796 33.850250772825724 23.542135471619943 17.365555644316537 8 24.358242681535806 35.916812657985545 21.288028602338496 18.436916058140152 9 24.575148920305093 17.202117455048953 22.82682358743382 35.39591003721213 10-11 23.008532215613638 27.62177167091377 29.551508573154823 19.818187540317773 12-13 24.088847560018728 25.389911519039167 29.74290503049301 20.7783358904491 14-15 21.312195699529095 27.265227089761712 27.919645071456863 23.50293213925233 16-17 19.917044413146957 30.66360016802598 28.43151499578271 20.987840423044357 18-19 19.778323268357763 28.850638840422132 31.59494090917383 19.776096982046276 20-21 20.996488923811036 27.94841372609767 31.369170146165086 19.68592720392621 22-23 20.545392698399922 27.949900647607194 31.51287496088433 19.99183169310855 24-25 20.36834422774269 28.172923143564333 31.351975270508536 20.106757358184446 26-27 20.15067779242907 28.127114913300876 31.46788686249143 20.254320431778623 28-29 20.12492495701455 28.18004593184022 31.52186223491152 20.173166876233715 30-31 20.412152982462754 28.47882734649727 31.17438303298397 19.934636638056002 32-33 20.040286527313103 28.337575127655846 31.503021705687196 20.11911663934385 34-35 20.329182542670416 28.152546063375716 31.3373650310033 20.18090636295057 36-37 20.644120393801416 28.356423584303897 30.901535640162457 20.097920381732227 38-39 20.455963920237547 28.541398743536885 30.94002167448588 20.06261566173969 40-41 20.344866640729695 28.344162019625642 31.03503652790386 20.275934811740804 42-43 20.379858238327493 28.424529907447145 30.72220348280043 20.473408371424938 44-45 20.543670725439437 28.59228106470774 30.502377477582982 20.36167073226984 46-47 20.81636009346609 28.783912758065878 30.041458629911084 20.358268518556947 48-49 20.760979343916333 28.687725026950172 29.957963484027044 20.59333214510645 50-51 20.734216951366484 28.81808040162685 29.714946854872043 20.732755792134622 52-53 20.720258832250206 28.946000107130548 29.593586554847967 20.74015450577128 54-55 20.732703887419266 29.342917207427206 29.48606230918832 20.43831659596521 56-57 20.68656665187787 29.019427703480044 29.48067727923704 20.813328365405045 58-59 20.668864194915713 28.663127565434106 29.766393380612616 20.90161485903757 60-61 20.824598983602886 28.96230759484571 29.527782937491658 20.68531048405974 62-63 20.629435879634784 28.961563508903588 29.604084537758567 20.804916073703065 64-65 20.70367658047026 28.844323884145396 29.63813884166579 20.813860693718556 66-67 20.59043446659156 28.907311894640014 29.48219111605469 21.02006252271373 68-69 20.94723484192205 29.070384898827413 29.284376563773606 20.69800369547693 70-71 21.004188289389322 28.831687378139836 29.232896521482317 20.931227810988528 72-73 20.75738747471125 28.98296746194265 29.44277194042824 20.816873122917855 74-75 20.815196555018247 29.11990765842904 29.301503468406914 20.763392318145797 76-77 20.930165500353993 29.16459825988827 29.077618179321025 20.827618060436716 78-79 20.94327815963059 29.119225906689383 29.259835739045688 20.67766019463434 80-81 20.905791990296542 29.0566194678168 29.24411039708709 20.79347814479956 82-83 20.725145462595545 29.113582505414033 29.433268830285016 20.72800320170541 84-85 20.709470645409198 29.07380725244672 29.249989743510817 20.966732358633262 86-87 20.797073672742417 29.214522646021862 29.146003856678405 20.842399824557315 88-89 20.677004290553835 29.12168295637328 29.216655871801002 20.98465688127188 90-91 20.584673281289906 28.97600603951379 29.27212861563755 21.16719206355876 92-93 20.718426269861514 29.212511156502973 29.132094853974795 20.93696771966072 94-95 20.634202162200392 28.981559299444097 29.298211244178628 21.086027294176883 96-97 20.58331722371368 29.066281257705384 29.242672947285755 21.10772857129518 98-99 20.591927127531402 29.009592628230042 29.174614123432946 21.22386612080561 100-101 20.673166670860162 29.040361398019687 28.992278867265153 21.294193063855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5466.0 1 3558.0 2 2356.5 3 3124.0 4 2642.0 5 2387.5 6 3791.5 7 7071.5 8 11364.0 9 13961.5 10 12847.0 11 11020.5 12 11228.0 13 12370.0 14 13421.0 15 14481.0 16 15621.5 17 16937.0 18 18825.0 19 21409.5 20 25192.5 21 30562.0 22 38732.5 23 51840.0 24 67967.0 25 86974.5 26 113221.0 27 144534.0 28 183574.5 29 225999.0 30 259238.5 31 291413.5 32 326129.0 33 357431.5 34 383321.5 35 402871.0 36 416736.0 37 426185.0 38 433809.5 39 436557.5 40 435725.5 41 426778.0 42 411704.5 43 396326.5 44 379004.0 45 364446.0 46 353495.5 47 343593.0 48 333737.5 49 323217.0 50 307560.5 51 284519.5 52 261396.5 53 243395.5 54 229189.5 55 216650.5 56 197506.5 57 176451.5 58 159014.0 59 143105.5 60 126180.0 61 106882.0 62 89336.0 63 73778.5 64 60272.5 65 49393.5 66 39744.5 67 31040.5 68 23920.0 69 18050.5 70 13747.0 71 10384.5 72 7886.0 73 6111.0 74 4737.5 75 3646.5 76 2690.0 77 1996.5 78 1486.5 79 1107.5 80 812.5 81 603.0 82 461.5 83 363.0 84 308.5 85 263.0 86 229.0 87 189.0 88 154.5 89 134.0 90 115.0 91 101.0 92 90.0 93 86.0 94 71.0 95 50.5 96 41.0 97 27.5 98 18.0 99 13.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014100710542671596 2 0.004642600589463303 3 0.004028774440763313 4 0.005870252886863282 5 0.0028097675820774174 6 0.007348622906971707 7 0.0012881703683985698 8 0.004028774440763313 9 0.010469626282756161 10-11 0.007469659048968889 12-13 0.008887510998078725 14-15 0.003531661714703462 16-17 0.00626362034835412 18-19 0.003855865666481625 20-21 9.639664166204063E-4 22-23 0.0011801023844725154 24-25 0.007309718432758327 26-27 0.0010806798392605451 28-29 7.694440455535083E-4 30-31 5.922125519147788E-4 32-33 0.0012708794909704012 34-35 0.001742055900887999 36-37 0.0013054612458267387 38-39 0.0017117968653887036 40-41 0.0025331135432267184 42-43 0.002312654856017567 44-45 0.007422109136041425 46-47 0.01321887579383499 48-49 0.004664214186248514 50-51 0.005481208144729486 52-53 0.012661244996776549 54-55 0.007837090194317473 56-57 0.005217522263949912 58-59 0.006639696932416789 60-61 0.0013184294038978653 62-63 0.002122455204307711 64-65 0.002105164326879542 66-67 0.002390463804444326 68-69 0.0019106419558126441 70-71 0.0022218777495196813 72-73 0.0011887478231865998 74-75 0.001482692739465468 76-77 0.008009998968599162 78-79 0.006445174561349892 80-81 0.012393236396639933 82-83 0.014753441165584963 84-85 0.00816561686545268 86-87 0.0053817855995175155 88-89 0.002917835566003472 90-91 0.002584986175511224 92-93 9.380301004781533E-4 94-95 0.0012968158071126543 96-97 0.001772314936387294 98-99 0.006259297628997078 100-101 0.010910543657174466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1566793E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.032222080662926 #Duplication Level Percentage of deduplicated Percentage of total 1 68.36636029090475 34.20520920921161 2 17.124179182784218 17.135214716442498 3 6.373395556677629 9.566254256988161 4 2.922445493837304 5.848657678652025 5 1.5931864601090096 3.9855329394039596 6 0.9115306718312286 2.7363543003837543 7 0.5902132360956311 2.0670775792298346 8 0.39540027228291125 1.5826203386890563 9 0.28423032715996094 1.2798607365473982 >10 1.310369159146824 11.532674610678034 >50 0.07796337037877712 2.7070395089469743 >100 0.04630125464527027 4.3161866263496735 >500 0.0030914484144281573 1.0574081594649078 >1k 0.0012293854581257768 1.0079785872144753 >5k 5.194513704526427E-5 0.17070642705516068 >10k+ 5.194513704526427E-5 0.801224324742484 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39903 0.3449789410081083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33851 0.29265674591046975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18733 0.16195500343094235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7290877428168725E-5 0.0 5 0.0 8.645438714084363E-6 0.0 1.7290877428168725E-5 0.0 6 0.0 8.645438714084363E-6 0.0 4.322719357042181E-5 0.0 7 0.0 8.645438714084363E-6 0.0 6.051807099859054E-5 0.0 8 0.0 8.645438714084363E-6 0.0 6.91635097126749E-5 0.0 9 8.645438714084363E-6 8.645438714084363E-6 0.0 6.91635097126749E-5 0.0 10-11 8.645438714084363E-6 8.645438714084363E-6 0.0 7.780894842675926E-5 0.0 12-13 1.2968158071126543E-5 8.645438714084363E-6 0.0 8.645438714084362E-5 0.0 14-15 1.7290877428168725E-5 8.645438714084363E-6 0.0 8.645438714084362E-5 0.0 16-17 1.7290877428168725E-5 1.2968158071126543E-5 0.0 9.07771064978858E-5 0.0 18-19 1.7290877428168725E-5 2.5936316142253086E-5 0.0 1.0374526456901235E-4 0.0 20-21 1.7290877428168725E-5 2.5936316142253086E-5 0.0 1.1239070328309672E-4 0.0 22-23 1.7290877428168725E-5 2.5936316142253086E-5 0.0 2.1181324849506685E-4 0.0 24-25 1.7290877428168725E-5 2.5936316142253086E-5 0.0 3.501402679204167E-4 0.0 26-27 1.7290877428168725E-5 2.5936316142253086E-5 0.0 6.527306229133693E-4 0.0 28-29 1.7290877428168725E-5 2.5936316142253086E-5 0.0 0.0016080516008196914 0.0 30-31 1.7290877428168725E-5 2.5936316142253086E-5 0.0 0.004076324353690776 0.0 32-33 1.7290877428168725E-5 2.5936316142253086E-5 0.0 0.009341396530568154 0.0 34-35 1.7290877428168725E-5 2.5936316142253086E-5 0.0 0.019015642451628553 0.0 36-37 1.7290877428168725E-5 2.5936316142253086E-5 0.0 0.03473305003383392 0.0 38-39 1.7290877428168725E-5 4.322719357042181E-5 0.0 0.05591869760269765 0.0 40-41 2.5936316142253086E-5 4.322719357042181E-5 0.0 0.07862594238524023 0.0 42-43 3.458175485633745E-5 4.322719357042181E-5 0.0 0.10203346770362365 0.0 44-45 3.458175485633745E-5 5.187263228450617E-5 0.0 0.13149712284122314 0.0 46-47 3.458175485633745E-5 6.051807099859054E-5 0.0 0.16669270384626056 0.0 48-49 3.458175485633745E-5 6.051807099859054E-5 0.0 0.20421823058474375 0.0 50-51 4.3227193570421815E-5 6.051807099859054E-5 0.0 0.24296276418191282 0.0 52-53 6.484079035563272E-5 6.051807099859054E-5 0.0 0.2813571575111615 0.0 54-55 9.509982585492799E-5 6.051807099859054E-5 0.0 0.3257903897821981 0.0 56-57 1.2103614199718107E-4 6.91635097126749E-5 0.0 0.3794137234063063 0.0 58-59 1.2103614199718107E-4 7.780894842675926E-5 0.0 0.44103841056029963 0.0 60-61 1.2968158071126543E-4 7.780894842675926E-5 0.0 0.5059699780224303 0.0 62-63 1.383270194253498E-4 8.645438714084362E-5 0.0 0.5719087390947517 0.0 64-65 1.4697245813943415E-4 8.645438714084362E-5 0.0 0.6436140077893674 0.0 66-67 1.4697245813943415E-4 9.509982585492799E-5 0.0 0.7289358424586659 0.0 68-69 1.4697245813943415E-4 1.123907032830967E-4 0.0 0.828764723290198 0.0 70-71 1.4697245813943415E-4 1.123907032830967E-4 0.0 0.9362621082611231 0.0 72-73 1.4697245813943415E-4 1.123907032830967E-4 0.0 1.0509611436808803 0.0 74-75 1.5561789685351852E-4 1.2103614199718107E-4 0.0 1.1697581170511135 0.0 76-77 1.5561789685351852E-4 1.2968158071126543E-4 0.0 1.302919486844798 0.0 78-79 1.599406162105607E-4 1.2968158071126543E-4 0.0 1.4603615712669882 0.0 80-81 1.642633355676029E-4 1.383270194253498E-4 0.0 1.6348913653075663 0.0 82-83 1.7290877428168723E-4 1.383270194253498E-4 0.0 1.8199037537889717 0.0 84-85 1.7290877428168723E-4 1.383270194253498E-4 0.0 2.006675489048693 0.0 86-87 1.7290877428168723E-4 1.383270194253498E-4 0.0 2.2107770062107965 0.0 88-89 1.7290877428168723E-4 1.383270194253498E-4 0.0 2.4431145262130998 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 25610 0.0 49.468002 1 GTATCAA 45530 0.0 39.134552 1 ATCAACG 54230 0.0 32.47907 3 TCAACGC 54875 0.0 32.098003 4 TATCAAC 56430 0.0 31.320496 2 CAACGCA 57015 0.0 30.876173 5 AACGCAG 59885 0.0 29.49967 6 CGCAGAG 65630 0.0 26.470585 8 ACGCAGA 66180 0.0 26.46594 7 GCAGAGT 70405 0.0 23.946577 9 GTACATG 35510 0.0 23.369701 1 GTGGTAT 13090 0.0 22.753008 1 TACATGG 34945 0.0 22.400164 2 GAGTACT 46255 0.0 22.173534 12-13 TGGTATC 13115 0.0 21.621462 2 ACATGGG 35230 0.0 21.3438 3 AGAGTAC 61590 0.0 21.164469 10-11 CAGAGTA 68555 0.0 20.708448 10-11 GTACTTT 51575 0.0 19.77365 14-15 AGTACTT 48865 0.0 19.618427 12-13 >>END_MODULE