FastQCFastQC Report
Wed 25 May 2016
SRR2049417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566793
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386090.33379174331208317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331050.2862072486297628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222480.1923437205109489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248300.047.012351
GTATCAA432150.038.0761341
ATCAACG538500.030.2367823
TCAACGC544650.029.912544
CAACGCA563500.028.970675
TATCAAC565950.028.8798272
AACGCAG596200.027.6202326
CGCAGAG676300.024.0746158
ACGCAGA680600.024.0690637
GTACATG343800.022.0641561
GCAGAGT734650.021.7745679
TACATGG334550.021.3963742
AGAGTAC634600.021.28193510-11
ACATGGG332400.020.6055743
GAGTACT480000.020.2848812-13
GTGGTAT132600.019.9651221
AGTACTT506200.019.63843512-13
CAGAGTA717600.018.60197610-11
TGGTATC137800.018.4758132
GTACTTT528300.018.3047814-15
Produced by FastQC (version 0.11.5)