##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11566793 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05067990755951 33.0 31.0 34.0 30.0 34.0 2 32.24616166296051 34.0 31.0 34.0 30.0 34.0 3 32.31874591340919 34.0 31.0 34.0 30.0 34.0 4 35.956053333019796 37.0 35.0 37.0 35.0 37.0 5 35.84005635788589 37.0 35.0 37.0 35.0 37.0 6 35.86808063393198 37.0 35.0 37.0 35.0 37.0 7 35.79353144817237 37.0 35.0 37.0 35.0 37.0 8 35.785933404358495 37.0 35.0 37.0 33.0 37.0 9 37.396537398049745 39.0 37.0 39.0 34.0 39.0 10-11 37.391325322412186 39.0 37.0 39.0 34.0 39.0 12-13 37.370185236305346 39.0 37.0 39.0 34.0 39.0 14-15 38.597677333725954 40.0 38.0 41.0 34.0 41.0 16-17 38.59329262657333 40.0 38.0 41.0 34.0 41.0 18-19 38.635188422581784 40.0 38.0 41.0 34.0 41.0 20-21 38.632000546737544 40.0 38.0 41.0 34.0 41.0 22-23 38.55247811558485 40.0 38.0 41.0 34.0 41.0 24-25 38.45775795417104 40.0 38.0 41.0 34.0 41.0 26-27 38.27801331795252 40.0 38.0 41.0 34.0 41.0 28-29 38.09442306091239 40.0 38.0 41.0 33.0 41.0 30-31 37.91116033631794 40.0 37.0 41.0 33.0 41.0 32-33 37.6607639213393 40.0 37.0 41.0 33.0 41.0 34-35 37.475496967914964 40.0 36.5 41.0 32.5 41.0 36-37 37.275716311340574 39.5 36.0 41.0 32.0 41.0 38-39 37.410383932694224 40.0 37.0 41.0 33.0 41.0 40-41 37.313799685012086 40.0 37.0 41.0 32.0 41.0 42-43 37.12508052145482 40.0 37.0 41.0 31.5 41.0 44-45 36.89455832744651 40.0 36.0 41.0 31.0 41.0 46-47 36.57744903881309 40.0 36.0 41.0 30.5 41.0 48-49 36.320258649048185 39.0 36.0 41.0 30.0 41.0 50-51 36.10552458231076 39.0 35.5 41.0 29.5 41.0 52-53 35.93914596725298 39.0 35.0 40.5 29.0 41.0 54-55 35.84390081157326 39.0 35.0 40.0 29.0 41.0 56-57 35.767360192233056 39.0 35.0 40.5 28.5 41.0 58-59 35.55163483949268 39.0 35.0 40.0 28.0 41.0 60-61 35.32567609708239 38.5 35.0 40.0 28.0 41.0 62-63 35.06670228299235 38.0 34.5 40.0 27.0 41.0 64-65 34.82776587252837 38.0 34.0 40.0 27.0 41.0 66-67 34.51146259814626 37.0 34.0 40.0 26.0 41.0 68-69 34.21056355032894 37.0 34.0 40.0 26.0 41.0 70-71 33.889810900912636 36.5 34.0 39.0 26.0 41.0 72-73 33.53162864590038 36.0 33.0 39.0 25.5 40.5 74-75 33.15918586941082 35.5 33.0 39.0 25.0 40.0 76-77 31.115716473874826 33.5 30.0 36.0 22.0 38.5 78-79 32.440587378022585 35.0 32.5 37.0 24.5 39.0 80-81 32.44677245456022 35.0 33.0 37.0 25.0 39.0 82-83 32.157969974910074 35.0 33.0 36.5 24.0 39.0 84-85 31.873164411259026 35.0 33.0 36.0 24.0 37.5 86-87 31.593715950479965 35.0 32.5 36.0 23.5 37.0 88-89 31.31200221184904 35.0 32.0 35.0 22.5 37.0 90-91 31.035898714535655 35.0 32.0 35.0 20.0 36.0 92-93 30.78340854720924 35.0 32.0 35.0 19.5 36.0 94-95 30.52287163779969 34.0 32.0 35.0 18.0 36.0 96-97 30.283461025022234 34.0 31.5 35.0 11.0 35.5 98-99 30.00279524324504 34.0 31.0 35.0 2.0 35.0 100-101 29.396104823523686 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.388501993049843 1101 2 0.3944354903256624 1101 3 0.3691758894088366 1101 4 0.2976943241817196 1101 5 0.32566998296825744 1101 6 0.3015423829442625 1101 7 0.34067946866642984 1101 8 0.32229807834743696 1101 9 0.41534891967881293 1101 10-11 0.3921117998127315 1101 12-13 0.33858535082477204 1101 14-15 0.4671436316602353 1101 16-17 0.42811298759657035 1101 18-19 0.41226793087720637 1101 20-21 0.38163465817854814 1101 22-23 0.31035207207131776 1101 24-25 0.27008379238925073 1101 26-27 0.25889655647494436 1101 28-29 0.266054410491968 1101 30-31 0.24447906325252688 1101 32-33 0.24767477608683208 1101 34-35 0.19211470772765438 1101 36-37 0.1476136504460186 1101 38-39 0.2932433381595203 1101 40-41 0.3460351072421233 1101 42-43 0.3573266042014609 1101 44-45 0.311448174672158 1101 46-47 0.4068122573260524 1101 48-49 0.2746546938725771 1101 50-51 0.3069442001074094 1101 52-53 0.2544047299121104 1101 54-55 0.2220222241818206 1101 56-57 0.17654587569909808 1101 58-59 0.1970998433031923 1101 60-61 0.23251160148787164 1101 62-63 0.1801928143261975 1101 64-65 0.0552100095476078 1101 66-67 0.12635590111378292 1101 68-69 0.058454113756404524 1101 70-71 0.04415575410065742 1101 72-73 0.030138159061785075 1101 74-75 0.049110956277139906 1101 76-77 0.05387168011542798 1101 78-79 0.06760623653170228 1101 80-81 0.003986812663598016 1101 82-83 -0.005243424552205056 1101 84-85 -0.05108609744465298 1101 86-87 -0.0184688336560761 1101 88-89 -0.03722878579289457 1101 90-91 -0.04435487978473418 1101 92-93 -0.07042272888415013 1101 94-95 -0.0263473766232849 1101 96-97 -0.13028610336256463 1101 98-99 -0.25894415612474475 1101 100-101 -0.32244710061621973 1102 1 0.34580588340010365 1102 2 0.34188283627336347 1102 3 0.31012656340698896 1102 4 0.2943980558668855 1102 5 0.2927024041271906 1102 6 0.2900880486902224 1102 7 0.2835404355858344 1102 8 0.2694709625119671 1102 9 0.33996733152757486 1102 10-11 0.3318301147299678 1102 12-13 0.28077726233029665 1102 14-15 0.3194650646967929 1102 16-17 0.38076307151543887 1102 18-19 0.3507302614081027 1102 20-21 0.35237525560997796 1102 22-23 0.2730654955696039 1102 24-25 0.2787028069344615 1102 26-27 0.3038206847195113 1102 28-29 0.3301393670902968 1102 30-31 0.30206846019630973 1102 32-33 0.2839079653624097 1102 34-35 0.2333434530856806 1102 36-37 0.1591268964682655 1102 38-39 0.3047118571649534 1102 40-41 0.29736061524918256 1102 42-43 0.3514715231485681 1102 44-45 0.2849048011628881 1102 46-47 0.3096310385237402 1102 48-49 0.0487806755785698 1102 50-51 0.16316485306077766 1102 52-53 0.2176281785452261 1102 54-55 0.17491318176400483 1102 56-57 0.2150103885607635 1102 58-59 0.22443406254959797 1102 60-61 0.2707201110820918 1102 62-63 0.22342143421415273 1102 64-65 0.22725512888960253 1102 66-67 0.23493837497397863 1102 68-69 0.18264584206415435 1102 70-71 0.1460132963315104 1102 72-73 0.1481009874862238 1102 74-75 0.11545219731834067 1102 76-77 0.1254901828933086 1102 78-79 0.20604975293141337 1102 80-81 0.2107928174428224 1102 82-83 0.27601887846712003 1102 84-85 0.2801597172962147 1102 86-87 0.1990215221482039 1102 88-89 0.18232099817150882 1102 90-91 0.16092529146053636 1102 92-93 0.14255329590393728 1102 94-95 0.1481273139529904 1102 96-97 0.11628791239908054 1102 98-99 0.0897112283143926 1102 100-101 0.05213258741939697 1103 1 0.3482477660740244 1103 2 0.37056591945946593 1103 3 0.3194103116204232 1103 4 0.2627621010683896 1103 5 0.27233350079161056 1103 6 0.29997802373200244 1103 7 0.26061937855185136 1103 8 0.2676893754836769 1103 9 0.34171629833602424 1103 10-11 0.34887014986376386 1103 12-13 0.32081196027310455 1103 14-15 0.38868887597078583 1103 16-17 0.420487690029006 1103 18-19 0.38595379425559884 1103 20-21 0.3614691435916484 1103 22-23 0.270473572118874 1103 24-25 0.3339739308934213 1103 26-27 0.27518671920387305 1103 28-29 0.2820748762886396 1103 30-31 0.2731184203493342 1103 32-33 0.3371732057837562 1103 34-35 0.26506451026035904 1103 36-37 0.30486811648681567 1103 38-39 0.39302085983637625 1103 40-41 0.3935598954908386 1103 42-43 0.4464454691052211 1103 44-45 0.42731617804340516 1103 46-47 0.48196479931500136 1103 48-49 0.4551246484408722 1103 50-51 0.5116899909163877 1103 52-53 0.4158347362921617 1103 54-55 0.4569129713001203 1103 56-57 0.40644511177697495 1103 58-59 0.4060034364666407 1103 60-61 0.3679760998707735 1103 62-63 0.4227928687289264 1103 64-65 0.4502339946727858 1103 66-67 0.4109070383666591 1103 68-69 0.4382669344909971 1103 70-71 0.41622505523716313 1103 72-73 0.4544173463307217 1103 74-75 0.4530941447861423 1103 76-77 0.34343143456555225 1103 78-79 0.5032831086706935 1103 80-81 0.4722302036496018 1103 82-83 0.4761115899339714 1103 84-85 0.4665138680341414 1103 86-87 0.4841634318037684 1103 88-89 0.4655364911518056 1103 90-91 0.4779347701807062 1103 92-93 0.406974094078965 1103 94-95 0.4341341717539571 1103 96-97 0.39745355412864924 1103 98-99 0.3801823000748854 1103 100-101 0.36746615455391307 1104 1 0.3359066249455367 1104 2 0.31978671330411856 1104 3 0.3203167934383231 1104 4 0.24540909221973806 1104 5 0.23962407487970694 1104 6 0.2565367905693421 1104 7 0.29934576416216174 1104 8 0.2890524763260416 1104 9 0.3686685921353501 1104 10-11 0.30823721688381767 1104 12-13 0.2883231989463937 1104 14-15 0.33961893738003823 1104 16-17 0.36555294523942194 1104 18-19 0.3573469138688452 1104 20-21 0.35141437219829186 1104 22-23 0.3217412636530881 1104 24-25 0.2964544824893025 1104 26-27 0.33723905495155293 1104 28-29 0.3609628930379998 1104 30-31 0.3341008410620603 1104 32-33 0.336186204479624 1104 34-35 0.3626243333610759 1104 36-37 0.39997651568715753 1104 38-39 0.41595180423112055 1104 40-41 0.4887639157635775 1104 42-43 0.4754496453815875 1104 44-45 0.4897271448613836 1104 46-47 0.5055082464965963 1104 48-49 0.5036035209128755 1104 50-51 0.4577685543791574 1104 52-53 0.4742496345489684 1104 54-55 0.4571989571233388 1104 56-57 0.43483953641709405 1104 58-59 0.39737193194357445 1104 60-61 0.46024196565221587 1104 62-63 0.4992911468358088 1104 64-65 0.5016228260601494 1104 66-67 0.49306403918539843 1104 68-69 0.4709560241950399 1104 70-71 0.41792854538548596 1104 72-73 0.4112134779809864 1104 74-75 0.44115951218400795 1104 76-77 0.3045792247105261 1104 78-79 0.4567794582358786 1104 80-81 0.4184247169570767 1104 82-83 0.42219414628288376 1104 84-85 0.35953638797150234 1104 86-87 0.3280625292955115 1104 88-89 0.32380237222072594 1104 90-91 0.3428850772626504 1104 92-93 0.31016170654897834 1104 94-95 0.2575797805844786 1104 96-97 0.21881957565198462 1104 98-99 0.19472460557359028 1104 100-101 0.2568699076468022 1105 1 0.292863868893221 1105 2 0.29979224016873474 1105 3 0.2564786466127842 1105 4 0.20227950970812714 1105 5 0.24363802891324582 1105 6 0.2665300157967323 1105 7 0.25529263679492686 1105 8 0.2394862427011688 1105 9 0.29584062872702077 1105 10-11 0.23973096624998647 1105 12-13 0.2614055553858634 1105 14-15 0.3470013427426153 1105 16-17 0.33971113225938865 1105 18-19 0.3122700924546109 1105 20-21 0.28226790439072147 1105 22-23 0.18912242517344424 1105 24-25 0.24028262049439775 1105 26-27 0.270688646728523 1105 28-29 0.2992518637790482 1105 30-31 0.3114669640857528 1105 32-33 0.3419946579193791 1105 34-35 0.31818698493528785 1105 36-37 0.33694305098805444 1105 38-39 0.4382244935916546 1105 40-41 0.468078544619992 1105 42-43 0.4870333361852417 1105 44-45 0.4948105476326461 1105 46-47 0.4832746649814581 1105 48-49 0.454780013265065 1105 50-51 0.4488812901617507 1105 52-53 0.5163167237328352 1105 54-55 0.5002902413179626 1105 56-57 0.4873362302014428 1105 58-59 0.5106289346619661 1105 60-61 0.5430168151394668 1105 62-63 0.5759320870527418 1105 64-65 0.5700140728378642 1105 66-67 0.5456542425761057 1105 68-69 0.5095131107442725 1105 70-71 0.43776810092114005 1105 72-73 0.5109856578105365 1105 74-75 0.49619342772145814 1105 76-77 0.5116825903506452 1105 78-79 0.5766919678906817 1105 80-81 0.5339202726388663 1105 82-83 0.6002971804737669 1105 84-85 0.5784053450851729 1105 86-87 0.5319240821646964 1105 88-89 0.5809566357795966 1105 90-91 0.6597551403010264 1105 92-93 0.612042498053146 1105 94-95 0.6191059897890412 1105 96-97 0.6229595416758968 1105 98-99 0.5835790344475988 1105 100-101 0.7629948903236219 1106 1 0.2728559599540432 1106 2 0.22817723768468312 1106 3 0.19905606754814897 1106 4 0.19248866460658576 1106 5 0.1870171471692217 1106 6 0.1935180682574682 1106 7 0.22615172731987343 1106 8 0.2029942065643482 1106 9 0.30282819199915423 1106 10-11 0.2586252616189384 1106 12-13 0.233967472816758 1106 14-15 0.2543189079064021 1106 16-17 0.25345645499681524 1106 18-19 0.2506701879634221 1106 20-21 0.2578925352592236 1106 22-23 0.2391126399385044 1106 24-25 0.2616456286754456 1106 26-27 0.260036531989158 1106 28-29 0.3142294795238243 1106 30-31 0.31292619510906405 1106 32-33 0.2883012313049207 1106 34-35 0.28837616575400915 1106 36-37 0.30162294265793577 1106 38-39 0.3211521558656756 1106 40-41 0.31860430094851466 1106 42-43 0.3134135900931412 1106 44-45 0.33096193061520296 1106 46-47 0.4214061419669193 1106 48-49 0.398109850596839 1106 50-51 0.4164379882399061 1106 52-53 0.4537752555265726 1106 54-55 0.4315087182582076 1106 56-57 0.40974799569642784 1106 58-59 0.48955052336104643 1106 60-61 0.5348628574215439 1106 62-63 0.5236203073780175 1106 64-65 0.529070105896011 1106 66-67 0.5569360852395704 1106 68-69 0.529353727860375 1106 70-71 0.48354702207975464 1106 72-73 0.5455085818189644 1106 74-75 0.4866236025363193 1106 76-77 0.4452537879848464 1106 78-79 0.4879789679537154 1106 80-81 0.5280452623456426 1106 82-83 0.49082996274433555 1106 84-85 0.49485119298206826 1106 86-87 0.46294798448450436 1106 88-89 0.5019318047328873 1106 90-91 0.5570040439531532 1106 92-93 0.568348406850216 1106 94-95 0.5651392085624174 1106 96-97 0.5267338577616343 1106 98-99 0.5858675125653257 1106 100-101 0.6182843583899675 1107 1 0.2373879436258619 1107 2 0.24720663510366592 1107 3 0.20621086075144746 1107 4 0.17276112544055877 1107 5 0.16120799347361014 1107 6 0.13524724673567334 1107 7 0.19959391404396598 1107 8 0.21011726348046977 1107 9 0.2366043615661937 1107 10-11 0.1876687856456769 1107 12-13 0.21502237314275874 1107 14-15 0.22753938611624847 1107 16-17 0.26751501500888963 1107 18-19 0.21501965969849834 1107 20-21 0.21160960829556075 1107 22-23 0.22137892170038498 1107 24-25 0.24869658001166783 1107 26-27 0.2447220833684014 1107 28-29 0.3101797935094126 1107 30-31 0.3285728352545263 1107 32-33 0.33663337627175594 1107 34-35 0.28239758101592116 1107 36-37 0.2855873208535513 1107 38-39 0.30311890873888814 1107 40-41 0.23841203505340758 1107 42-43 0.2583405106448211 1107 44-45 0.23740360350561218 1107 46-47 0.22300025821768088 1107 48-49 0.2142021465650359 1107 50-51 0.2618370624341395 1107 52-53 0.2668818089510765 1107 54-55 0.2902980589501354 1107 56-57 0.3096197267881351 1107 58-59 0.3760527282693005 1107 60-61 0.39641488151215043 1107 62-63 0.3826568559272445 1107 64-65 0.37554407710570814 1107 66-67 0.365199354543293 1107 68-69 0.4331842284236558 1107 70-71 0.42053489966661317 1107 72-73 0.5008931393489391 1107 74-75 0.3772963218767771 1107 76-77 0.40776003765744306 1107 78-79 0.49381882664860655 1107 80-81 0.5470521233236241 1107 82-83 0.5325662352161444 1107 84-85 0.4834024970945663 1107 86-87 0.48630224302469927 1107 88-89 0.4691793340827317 1107 90-91 0.4867851396594993 1107 92-93 0.5187153923204271 1107 94-95 0.5696525710667331 1107 96-97 0.5638537591517263 1107 98-99 0.6486024303898361 1107 100-101 0.7496784070497888 1108 1 0.12284990857314426 1108 2 0.12696570803922214 1108 3 0.10397330726480902 1108 4 0.10235846333988974 1108 5 0.06036762535492812 1108 6 0.10140319975362644 1108 7 0.13143472282418145 1108 8 0.10474566597552126 1108 9 0.23092668014713524 1108 10-11 0.10244146288543021 1108 12-13 0.07054062905989866 1108 14-15 0.10926709667668177 1108 16-17 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-1.1964613735223395 2316 52-53 -1.1693364985286934 2316 54-55 -1.8093272095618858 2316 56-57 -1.291206507982018 2316 58-59 -0.8704718057675436 2316 60-61 -0.8107627699203945 2316 62-63 -1.3817648461394967 2316 64-65 -1.6492967879339773 2316 66-67 -1.1764488889292153 2316 68-69 -1.1475524494355014 2316 70-71 -1.0776570186627055 2316 72-73 -1.1287032505904264 2316 74-75 -0.9072256516836106 2316 76-77 -0.9224936074256433 2316 78-79 -0.9336715518089136 2316 80-81 -0.8402927194260172 2316 82-83 -0.941513957541428 2316 84-85 -1.0552300246619808 2316 86-87 -1.1923999291635177 2316 88-89 -0.9005674790344962 2316 90-91 -0.8186390455873038 2316 92-93 -0.8838249532130753 2316 94-95 -1.1089279755011603 2316 96-97 -1.0641359779581698 2316 98-99 -1.0402746574430424 2316 100-101 -1.3359276916893243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 23.0 4 81.0 5 199.0 6 464.0 7 1337.0 8 3668.0 9 7999.0 10 13706.0 11 20417.0 12 29209.0 13 41214.0 14 52706.0 15 65345.0 16 77788.0 17 99200.0 18 82682.0 19 49272.0 20 39471.0 21 38577.0 22 40003.0 23 43664.0 24 51944.0 25 65354.0 26 81843.0 27 105269.0 28 134061.0 29 171872.0 30 218226.0 31 275816.0 32 355164.0 33 467294.0 34 646755.0 35 956927.0 36 1496829.0 37 2404366.0 38 2753504.0 39 674245.0 40 294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.99121530799185 22.83026930149762 16.30759223667116 22.870923153839367 2 14.241982267832674 22.378634943003746 39.82852276607977 23.550860023083807 3 16.57977548955555 27.160873907116827 31.972598739129094 24.28675186419853 4 11.836020790266314 18.770763703125482 39.28301782720639 30.11019767940181 5 12.06505887515921 38.27409275124351 36.07747472270801 13.583373650889275 6 28.843479036716396 39.81777192337526 17.865399386025494 13.473349653882853 7 25.032772696805417 33.89986316864147 23.450588248618264 17.61677588593485 8 24.433230925221096 36.26645140675133 20.961239728353377 18.339077939674198 9 24.246489172274966 17.022366105439126 22.683367240758628 36.047777481527284 10-11 22.887434629659566 27.928988941616474 29.42618479284227 19.757391635881692 12-13 23.971830394129125 25.460881853768804 29.783480174668988 20.783807577433087 14-15 21.05615438766064 27.408132507660444 27.88566169644785 23.650051408231064 16-17 19.74931599450254 30.72809809944727 28.525564519050356 20.997021386999837 18-19 19.640814656724867 28.85937805022069 31.774405612760535 19.725401680293903 20-21 20.78339182935763 27.998315515075316 31.544202102571596 19.67409055299546 22-23 20.30824793008745 27.968296830417906 31.703554304118697 20.01990093537595 24-25 20.25696479827623 28.151198950471745 31.515086752550133 20.0767494987019 26-27 20.052649403511673 28.11563657195959 31.64422477267958 20.187489251849158 28-29 20.00973462953743 28.161494728631965 31.74486123051718 20.08390941131343 30-31 20.325938544570217 28.439685997486396 31.383651925763523 19.850723532179863 32-33 19.96711655987669 28.266430653145203 31.691775309778553 20.07467747719956 34-35 20.17204786746777 28.147522391564618 31.554975937352125 20.12545380361549 36-37 20.511079261738182 28.362903133072788 31.07244189538026 20.053575709808772 38-39 20.30906017216725 28.501916161136414 31.154692581424147 20.034331085272186 40-41 20.18705156761186 28.336007569418104 31.22996214167016 20.246978721299875 42-43 20.20944141563871 28.416719883724063 30.914121134832477 20.45971756580475 44-45 20.369779193029913 28.58859795326864 30.69604678919931 20.34557606450214 46-47 20.609620690123222 28.805074940451984 30.196269174736447 20.389035194688347 48-49 20.546114825572662 28.697501166831664 30.11071943185273 20.64566457574295 50-51 20.5587708614539 28.818366616918702 29.823879238888573 20.798983282738824 52-53 20.565141943201624 28.94384931092866 29.686810984316796 20.80419776155292 54-55 20.631744713648818 29.295297988038506 29.565405337175974 20.507551961136702 56-57 20.616117523901767 28.970381144849377 29.58159323728261 20.831908093966245 58-59 20.528964227391064 28.68866476079303 29.83290219621 20.949468815605904 60-61 20.674148463101886 28.98380154542521 29.593299713450698 20.748750278022207 62-63 20.515786200614126 28.959946625068294 29.64733388818386 20.876933286133717 64-65 20.540943445913783 28.88207937605508 29.7108707767202 20.86610640131094 66-67 20.455872696880853 28.921711856787407 29.5543489063533 21.068066539978446 68-69 20.779434235528182 29.057501314847624 29.3492016542655 20.8138627953587 70-71 20.852635236957845 28.826281289552398 29.297130805879252 21.02395266761051 72-73 20.59906541462203 29.00019698733441 29.463962319180258 20.9367752788633 74-75 20.662772598429648 29.099523702090252 29.291982104537233 20.945721594942867 76-77 20.679430096835606 29.151321246162915 29.165448175760787 21.003800481240688 78-79 20.73203785555724 29.142301745421168 29.308328748249686 20.8173316507719 80-81 20.650485733610928 29.02741015690804 29.2985683574962 21.023535751984827 82-83 20.48679426690697 29.04494861165067 29.515944179948857 20.952312941493503 84-85 20.474913762214417 29.057734262476785 29.283278076338128 21.18407389897067 86-87 20.504138406049993 29.201696992239086 29.182490212777658 21.111674388933263 88-89 20.35858556690478 29.095152841025413 29.245133025225893 21.301128566843914 90-91 20.29282506360735 28.975801758622428 29.278330568347823 21.453042609422404 92-93 20.405878574118848 29.15893781888994 29.153655451953824 21.281528155037382 94-95 20.260508236722963 28.93233665919236 29.350220321445914 21.45693478263877 96-97 20.19195177352827 29.042706386347877 29.27476360168118 21.490578238442666 98-99 20.148963430161754 28.98532744535481 29.2179039515182 21.64780517296524 100-101 20.106768588333246 29.046114473490768 29.077484451932932 21.76963248624305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4906.0 1 3370.0 2 2286.5 3 2882.5 4 2442.5 5 2174.0 6 3894.5 7 7905.5 8 12070.5 9 14062.0 10 12782.5 11 10913.0 12 11379.5 13 12476.5 14 13565.0 15 14814.5 16 15908.5 17 17262.5 18 19353.5 19 22075.0 20 25696.0 21 30996.5 22 39276.0 23 52270.5 24 68122.0 25 87414.0 26 114363.5 27 146446.0 28 185830.5 29 227665.0 30 261085.5 31 294042.5 32 328744.5 33 359526.0 34 385593.0 35 405374.5 36 418701.0 37 428763.5 38 436431.0 39 438691.5 40 436690.0 41 427703.5 42 413265.5 43 398245.5 44 382384.5 45 368050.0 46 355255.5 47 345414.5 48 336224.5 49 325362.0 50 309490.5 51 285710.5 52 260560.5 53 241384.5 54 227998.0 55 213601.0 56 193264.5 57 172674.0 58 155726.0 59 140642.5 60 123642.0 61 103752.0 62 86258.0 63 70648.0 64 57076.0 65 46475.5 66 37278.0 67 28932.5 68 22012.0 69 16427.5 70 12206.0 71 8884.5 72 6577.5 73 5005.0 74 3831.5 75 2822.0 76 1962.0 77 1395.5 78 960.5 79 636.0 80 395.0 81 249.5 82 176.5 83 124.5 84 87.5 85 57.5 86 47.5 87 41.0 88 27.5 89 17.5 90 9.5 91 9.5 92 7.5 93 3.5 94 2.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049910117696409025 2 0.0021008416075225 3 2.2478140656619343E-4 4 2.939449162788683E-4 5 0.002463950033514043 6 0.0016339879169619445 7 0.0 8 8.645438714084363E-6 9 4.322719357042181E-5 10-11 2.5936316142253083E-5 12-13 0.0 14-15 5.662762357725257E-4 16-17 0.0 18-19 6.051807099859053E-5 20-21 4.5388553248942897E-4 22-23 0.0 24-25 6.267943067711163E-4 26-27 0.007348622906971707 28-29 0.0074999180844681846 30-31 0.0011584887876873045 32-33 0.006821251145412561 34-35 0.008144003268667469 36-37 0.004703118660461893 38-39 0.0026584724045809415 40-41 0.0021613596785210903 42-43 0.0014654018620372993 44-45 9.207392230499846E-4 46-47 6.570533422704116E-4 48-49 0.0018544466041710958 50-51 0.0013443657200401182 52-53 0.0021397460817358794 54-55 8.256393971950567E-4 56-57 1.9452237106689813E-4 58-59 8.1267123912393E-4 60-61 0.006229038593497782 62-63 0.0027189904755795316 64-65 0.0011325524715450515 66-67 0.005217522263949912 68-69 0.00703738711326467 70-71 0.002182973275306301 72-73 5.144036034880195E-4 74-75 0.003436561888848534 76-77 0.00200141906231053 78-79 0.002602277052939393 80-81 9.81257294048575E-4 82-83 1.5129517749647633E-4 84-85 7.132486939119599E-4 86-87 0.004880350154100622 88-89 0.002291041259232356 90-91 0.0010893252779746296 92-93 7.348622906971707E-5 94-95 4.927900067028086E-4 96-97 2.8962219692182614E-4 98-99 5.316944809161883E-4 100-101 1.2968158071126541E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1566793E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.64220807698768 #Duplication Level Percentage of deduplicated Percentage of total 1 66.3638107409939 32.28082290845254 2 18.325631689059644 17.827983795229567 3 6.7471573645530745 9.845898973643113 4 3.103929414689201 6.039279217823787 5 1.6179201586792282 3.9349604505213964 6 0.9651520216565525 2.8168275278006005 7 0.6327728177688213 2.1545626947160965 8 0.4127813339336921 1.6062876428399346 9 0.3016041081769261 1.3203620808134706 >10 1.3913288663417727 11.891094466860764 >50 0.08436625263410993 2.838661446866864 >100 0.049134821832944334 4.459307320111356 >500 0.0029670980101023444 0.9718069774229575 >1k 0.0013542195242238447 1.0670472357641383 >5k 3.56368583269688E-5 0.13070254303747125 >10k+ 5.345528749045319E-5 0.8143947180959464 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38609 0.33379174331208317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33105 0.2862072486297628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22248 0.1923437205109489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7290877428168725E-5 2 0.0 0.0 0.0 0.0 1.7290877428168725E-5 3 0.0 0.0 0.0 0.0 1.7290877428168725E-5 4 0.0 0.0 0.0 0.0 1.7290877428168725E-5 5 0.0 0.0 0.0 0.0 1.7290877428168725E-5 6 0.0 0.0 0.0 0.0 1.7290877428168725E-5 7 0.0 0.0 0.0 1.7290877428168725E-5 1.7290877428168725E-5 8 8.645438714084363E-6 0.0 0.0 1.7290877428168725E-5 1.7290877428168725E-5 9 8.645438714084363E-6 8.645438714084363E-6 0.0 3.458175485633745E-5 1.7290877428168725E-5 10-11 8.645438714084363E-6 1.7290877428168725E-5 0.0 4.3227193570421815E-5 1.7290877428168725E-5 12-13 8.645438714084363E-6 1.7290877428168725E-5 0.0 5.187263228450617E-5 2.5936316142253086E-5 14-15 8.645438714084363E-6 1.7290877428168725E-5 0.0 9.509982585492797E-5 2.5936316142253086E-5 16-17 8.645438714084363E-6 1.7290877428168725E-5 0.0 1.1671342264013889E-4 2.5936316142253086E-5 18-19 8.645438714084363E-6 2.1613596785210908E-5 0.0 1.2968158071126543E-4 3.458175485633745E-5 20-21 8.645438714084363E-6 2.5936316142253086E-5 0.0 1.3832701942534978E-4 3.458175485633745E-5 22-23 1.7290877428168725E-5 3.025903549929527E-5 0.0 2.3774956463731996E-4 3.458175485633745E-5 24-25 1.7290877428168725E-5 3.458175485633745E-5 0.0 3.5878570663450105E-4 3.458175485633745E-5 26-27 1.7290877428168725E-5 3.458175485633745E-5 0.0 6.700215003415381E-4 3.458175485633745E-5 28-29 1.7290877428168725E-5 5.619535164154836E-5 4.322719357042181E-6 0.0016166970395337758 3.458175485633745E-5 30-31 1.7290877428168725E-5 6.051807099859054E-5 8.645438714084363E-6 0.004084969792404861 3.458175485633745E-5 32-33 1.7290877428168725E-5 6.051807099859054E-5 8.645438714084363E-6 0.009397591882209701 3.458175485633745E-5 34-35 2.1613596785210908E-5 6.051807099859054E-5 8.645438714084363E-6 0.018929188064487713 3.458175485633745E-5 36-37 2.5936316142253086E-5 6.051807099859054E-5 8.645438714084363E-6 0.03448233231112548 3.458175485633745E-5 38-39 2.5936316142253086E-5 7.780894842675926E-5 8.645438714084363E-6 0.055512361983135694 3.458175485633745E-5 40-41 2.5936316142253086E-5 7.780894842675926E-5 8.645438714084363E-6 0.0781201842204663 3.458175485633745E-5 42-43 2.5936316142253086E-5 7.780894842675926E-5 8.645438714084363E-6 0.10141964155492365 3.458175485633745E-5 44-45 2.5936316142253086E-5 8.213166778380144E-5 8.645438714084363E-6 0.1308876194118802 3.458175485633745E-5 46-47 2.5936316142253086E-5 1.0374526456901235E-4 8.645438714084363E-6 0.16600539146849086 3.458175485633745E-5 48-49 3.458175485633745E-5 1.123907032830967E-4 8.645438714084363E-6 0.2033536867133353 3.458175485633745E-5 50-51 3.890447421337963E-5 1.123907032830967E-4 8.645438714084363E-6 0.24182588899101073 3.458175485633745E-5 52-53 6.051807099859053E-5 1.123907032830967E-4 8.645438714084363E-6 0.28014679609118964 3.458175485633745E-5 54-55 7.780894842675926E-5 1.123907032830967E-4 8.645438714084363E-6 0.3245324784492988 3.458175485633745E-5 56-57 9.509982585492799E-5 1.2535886135422325E-4 8.645438714084363E-6 0.377740831015131 3.458175485633745E-5 58-59 9.509982585492799E-5 1.383270194253498E-4 8.645438714084363E-6 0.4391364140432011 3.458175485633745E-5 60-61 9.509982585492799E-5 1.4697245813943415E-4 8.645438714084363E-6 0.5037826820277669 3.890447421337963E-5 62-63 1.0374526456901235E-4 1.5129517749647633E-4 8.645438714084363E-6 0.5691205851094594 4.322719357042181E-5 64-65 1.0374526456901235E-4 1.5561789685351852E-4 8.645438714084363E-6 0.6404454545006555 4.322719357042181E-5 66-67 1.0374526456901235E-4 1.5561789685351852E-4 8.645438714084363E-6 0.7251707538986822 4.322719357042181E-5 68-69 1.0374526456901235E-4 1.642633355676029E-4 8.645438714084363E-6 0.8240918636652355 4.322719357042181E-5 70-71 1.0374526456901235E-4 1.7723149363872942E-4 8.645438714084363E-6 0.9306641866937534 4.322719357042181E-5 72-73 1.0374526456901235E-4 1.9019965170985598E-4 8.645438714084363E-6 1.0444511283291749 5.187263228450617E-5 74-75 1.2968158071126543E-4 1.9884509042394032E-4 8.645438714084363E-6 1.1624354304602842 5.187263228450617E-5 76-77 1.4697245813943415E-4 1.9884509042394032E-4 8.645438714084363E-6 1.294861937963271 5.187263228450617E-5 78-79 1.4697245813943415E-4 1.9884509042394032E-4 8.645438714084363E-6 1.4515518692173361 5.187263228450617E-5 80-81 1.4697245813943415E-4 2.074905291380247E-4 8.645438714084363E-6 1.6251911830703636 5.187263228450617E-5 82-83 1.4697245813943415E-4 2.074905291380247E-4 8.645438714084363E-6 1.809468709261072 5.187263228450617E-5 84-85 1.4697245813943415E-4 2.074905291380247E-4 8.645438714084363E-6 1.9956871364430917 5.187263228450617E-5 86-87 1.4697245813943415E-4 2.1613596785210906E-4 8.645438714084363E-6 2.1991359229822818 5.187263228450617E-5 88-89 1.4697245813943415E-4 2.1613596785210906E-4 8.645438714084363E-6 2.4310930436811655 5.187263228450617E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24830 0.0 47.01235 1 GTATCAA 43215 0.0 38.076134 1 ATCAACG 53850 0.0 30.236782 3 TCAACGC 54465 0.0 29.91254 4 CAACGCA 56350 0.0 28.97067 5 TATCAAC 56595 0.0 28.879827 2 AACGCAG 59620 0.0 27.620232 6 CGCAGAG 67630 0.0 24.074615 8 ACGCAGA 68060 0.0 24.069063 7 GTACATG 34380 0.0 22.064156 1 GCAGAGT 73465 0.0 21.774567 9 TACATGG 33455 0.0 21.396374 2 AGAGTAC 63460 0.0 21.281935 10-11 ACATGGG 33240 0.0 20.605574 3 GAGTACT 48000 0.0 20.28488 12-13 GTGGTAT 13260 0.0 19.965122 1 AGTACTT 50620 0.0 19.638435 12-13 CAGAGTA 71760 0.0 18.601976 10-11 TGGTATC 13780 0.0 18.475813 2 GTACTTT 52830 0.0 18.30478 14-15 >>END_MODULE