FastQCFastQC Report
Wed 25 May 2016
SRR2049416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5627185
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240470.4273362258393851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211120.37517870835950834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113840.20230363849775687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.10276896885387632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121900.059.0757751
GTATCAA230700.042.684051
GTGGTAT46600.037.3086131
TGGTATC46950.036.2185022
ATCAACG284300.034.06833
TATAACG4050.034.011522
TCAACGC287300.033.7471244
TATCAAC297200.032.555572
CAACGCA299200.032.3240855
AACGCAG320950.030.2083666
CGCAGAG349700.027.4820358
ACGCAGA359750.026.8595547
GCAGAGT389300.024.1740119
GTACATG200100.023.454371
ATAACGC5750.023.1313113
TACATGG190450.022.7206342
GAGTACT263050.021.91504712-13
ACATGGG192700.021.7911743
CAGAGTA371700.021.48347310-11
AGAGTAC351150.020.74533710-11
Produced by FastQC (version 0.11.5)