##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5627185 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59181971092118 31.0 31.0 34.0 30.0 34.0 2 31.621955915790934 33.0 31.0 34.0 30.0 34.0 3 31.64768476600645 33.0 31.0 34.0 30.0 34.0 4 35.26613128944579 37.0 35.0 37.0 33.0 37.0 5 35.298264762932085 37.0 35.0 37.0 33.0 37.0 6 35.388776981741316 37.0 35.0 37.0 33.0 37.0 7 35.338582612798405 37.0 35.0 37.0 33.0 37.0 8 35.315584435201615 37.0 35.0 37.0 33.0 37.0 9 36.91482366405228 39.0 37.0 39.0 33.0 39.0 10-11 36.85655945201731 39.0 37.0 39.0 33.0 39.0 12-13 36.830375578553046 39.0 37.0 39.0 33.0 39.0 14-15 38.021782560907454 40.0 38.0 41.0 33.0 41.0 16-17 38.01565587411823 40.0 38.0 41.0 33.0 41.0 18-19 38.060770260796474 40.0 38.0 41.0 33.0 41.0 20-21 38.032547890286175 40.0 38.0 41.0 33.0 41.0 22-23 37.92806936327844 40.0 38.0 41.0 33.0 41.0 24-25 37.83050308457959 40.0 38.0 41.0 33.0 41.0 26-27 37.69470481244174 40.0 38.0 41.0 32.5 41.0 28-29 37.539235248174705 40.0 37.5 41.0 32.0 41.0 30-31 37.3345898526528 40.0 37.0 41.0 32.0 41.0 32-33 37.07183876129894 40.0 36.5 41.0 31.5 41.0 34-35 36.86445371886654 40.0 36.0 41.0 31.0 41.0 36-37 36.670012715060906 40.0 36.0 41.0 30.5 41.0 38-39 36.45922375041873 39.0 36.0 41.0 30.0 41.0 40-41 36.23863645854899 39.0 35.0 41.0 30.0 41.0 42-43 35.99927663654208 39.0 35.0 41.0 29.5 41.0 44-45 35.7045678256535 39.0 35.0 40.0 28.5 41.0 46-47 35.32502770035106 39.0 35.0 40.0 26.5 41.0 48-49 35.00729174533981 39.0 35.0 40.0 25.5 41.0 50-51 33.644884342704216 37.5 33.0 39.0 23.0 40.0 52-53 33.914476421159065 37.5 33.5 39.5 23.5 40.0 54-55 34.956195593356185 39.0 35.0 40.0 26.0 41.0 56-57 35.07779520310777 39.0 35.0 41.0 26.0 41.0 58-59 34.93527767436116 39.0 35.0 41.0 26.0 41.0 60-61 34.695796477279494 38.5 35.0 40.5 25.0 41.0 62-63 34.43454586973771 38.0 34.0 40.0 24.0 41.0 64-65 34.16647577785341 38.0 34.0 40.0 23.0 41.0 66-67 33.857472341854766 37.0 34.0 40.0 22.5 41.0 68-69 33.51768415291127 37.0 34.0 40.0 22.0 41.0 70-71 33.19017137343094 36.0 33.0 39.0 20.5 41.0 72-73 32.931160251528965 36.0 33.0 39.0 20.0 41.0 74-75 32.722555238542895 35.5 33.0 39.0 20.0 40.5 76-77 32.37702927840475 35.0 33.0 38.5 18.5 40.0 78-79 31.988434714693046 35.0 33.0 37.0 17.5 39.0 80-81 31.637709085448584 35.0 32.5 37.0 13.5 39.0 82-83 31.280176944600186 35.0 32.0 36.0 8.5 39.0 84-85 30.94318215946339 35.0 32.0 36.0 6.5 37.0 86-87 30.634746414059606 35.0 32.0 36.0 2.0 37.0 88-89 30.3339102944012 35.0 31.5 35.0 2.0 37.0 90-91 30.053853747477646 35.0 31.0 35.0 2.0 36.0 92-93 29.797064606903806 35.0 31.0 35.0 2.0 36.0 94-95 29.528806232601205 34.0 31.0 35.0 2.0 36.0 96-97 29.27185848696995 34.0 31.0 35.0 2.0 35.5 98-99 28.96729545945264 34.0 31.0 35.0 2.0 35.0 100-101 28.452141790255695 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.285635266006544 1101 2 0.2921960435207325 1101 3 0.2968295660360667 1101 4 0.2896188221580118 1101 5 0.25215643500279583 1101 6 0.29293274348004417 1101 7 0.3083122772808693 1101 8 0.28619352685515054 1101 9 0.3340331049817564 1101 10-11 0.3788458909787664 1101 12-13 0.32579565835304436 1101 14-15 0.43929950804992046 1101 16-17 0.44643404333093883 1101 18-19 0.4499154996065684 1101 20-21 0.3643724451506287 1101 22-23 0.37276515105525476 1101 24-25 0.34789870645491305 1101 26-27 0.33897096014458583 1101 28-29 0.3047372174789942 1101 30-31 0.2782003813708087 1101 32-33 0.31929596844332053 1101 34-35 0.3384406569594347 1101 36-37 0.28937328912155635 1101 38-39 0.21208240236196474 1101 40-41 0.13272931474002547 1101 42-43 0.11575941786618671 1101 44-45 0.0663781445569569 1101 46-47 -0.07307423348017039 1101 48-49 -0.14466240169103628 1101 50-51 -0.1280326959815028 1101 52-53 -0.15323701240250642 1101 54-55 0.08343043694868868 1101 56-57 0.10071271963091277 1101 58-59 0.066817830220252 1101 60-61 0.21607418183372573 1101 62-63 0.11412560862777354 1101 64-65 0.05072061592037613 1101 66-67 0.06382496439839969 1101 68-69 0.03258952025781525 1101 70-71 -0.0620278965780372 1101 72-73 -0.08629051571779911 1101 74-75 -0.0394315652652395 1101 76-77 -0.09164115260393402 1101 78-79 -0.11369265639204329 1101 80-81 -0.15656098576782185 1101 82-83 -0.17405591198106052 1101 84-85 -0.22200032081988397 1101 86-87 -0.31294700939211495 1101 88-89 -0.25633407519956464 1101 90-91 -0.3139327793654836 1101 92-93 -0.4311529294770189 1101 94-95 -0.5458818961959722 1101 96-97 -0.6102694937043687 1101 98-99 -0.5942064426353468 1101 100-101 -0.7617783668362712 1102 1 0.30688182649146256 1102 2 0.299205360430836 1102 3 0.24958121931096855 1102 4 0.27242049125688084 1102 5 0.26021168592256316 1102 6 0.3278478737200814 1102 7 0.3039586259004494 1102 8 0.29829240577103633 1102 9 0.3814587845332369 1102 10-11 0.36531722322493465 1102 12-13 0.3607283707788156 1102 14-15 0.49440496270326406 1102 16-17 0.4271352593588631 1102 18-19 0.37492012866641033 1102 20-21 0.36164081908415824 1102 22-23 0.36967844725354126 1102 24-25 0.4314814641548139 1102 26-27 0.4551882119500519 1102 28-29 0.38285727248925383 1102 30-31 0.3471813742038705 1102 32-33 0.401152478302528 1102 34-35 0.29872455980905954 1102 36-37 0.27036571010062005 1102 38-39 0.27806944429108427 1102 40-41 0.273619077732171 1102 42-43 0.29847555237869017 1102 44-45 0.3281387380985734 1102 46-47 0.2175401004516857 1102 48-49 0.10493300199595268 1102 50-51 0.047069012842122504 1102 52-53 0.3152634716570404 1102 54-55 0.3893427147538162 1102 56-57 0.3766610725097834 1102 58-59 0.32784732076543577 1102 60-61 0.4033396038831043 1102 62-63 0.3971994856755501 1102 64-65 0.33798808464606367 1102 66-67 0.337038566252005 1102 68-69 0.3074386431265239 1102 70-71 0.19876409709897302 1102 72-73 0.2521579058414716 1102 74-75 0.286864853078697 1102 76-77 0.2225703289479526 1102 78-79 0.2319632937492706 1102 80-81 0.21174696648995095 1102 82-83 0.029341783598777482 1102 84-85 0.009342301585096635 1102 86-87 -0.02707931616389203 1102 88-89 -0.0019979373244609633 1102 90-91 -0.07461354511362472 1102 92-93 -0.17917517396390892 1102 94-95 -0.22844662473801236 1102 96-97 -0.2628602699798428 1102 98-99 -0.25193973351502663 1102 100-101 -0.29036336077041724 1103 1 0.32651409263889164 1103 2 0.3277330183120064 1103 3 0.3286333444885763 1103 4 0.3343243821866366 1103 5 0.32125751727067353 1103 6 0.32086798821637075 1103 7 0.3055325728485414 1103 8 0.3588028719111662 1103 9 0.4167665818306432 1103 10-11 0.3844888331176861 1103 12-13 0.3442792478371217 1103 14-15 0.4250345173301966 1103 16-17 0.4187273362018331 1103 18-19 0.45514107851911234 1103 20-21 0.43535535020077987 1103 22-23 0.3882538705602201 1103 24-25 0.41275518685854706 1103 26-27 0.43142833324944974 1103 28-29 0.40414245382965674 1103 30-31 0.4264307323237375 1103 32-33 0.3676075218226984 1103 34-35 0.4601803667378874 1103 36-37 0.4009893049831703 1103 38-39 0.4051288770422232 1103 40-41 0.36105236270213936 1103 42-43 0.3265784723988645 1103 44-45 0.24125224172394155 1103 46-47 0.33351321736992645 1103 48-49 0.2678760034054619 1103 50-51 0.15663666113025698 1103 52-53 0.2936681943073154 1103 54-55 0.4538971905707996 1103 56-57 0.44009726398115845 1103 58-59 0.3945576508823976 1103 60-61 0.4273049240457354 1103 62-63 0.4686761915283455 1103 64-65 0.45505283677912445 1103 66-67 0.41660950904505256 1103 68-69 0.382618191033238 1103 70-71 0.38167274805632445 1103 72-73 0.4628060988672189 1103 74-75 0.38281978918638515 1103 76-77 0.3339906385165321 1103 78-79 0.34233115032165884 1103 80-81 0.3245382130941863 1103 82-83 0.2751781384180667 1103 84-85 0.2498519635187577 1103 86-87 0.24835996366921265 1103 88-89 0.2061152591749682 1103 90-91 0.23911428543693702 1103 92-93 0.30575701909343067 1103 94-95 0.2203084186059101 1103 96-97 0.2569468555897494 1103 98-99 0.2443775295927999 1103 100-101 0.21749477107231385 1104 1 0.08750040456289554 1104 2 0.07920399829412972 1104 3 0.060158986267467185 1104 4 0.03809672686931265 1104 5 0.015836153878296955 1104 6 0.03629627465041807 1104 7 0.005316420314215975 1104 8 0.005177192655438034 1104 9 0.07608666726489588 1104 10-11 0.08257072655912623 1104 12-13 -0.025501066804764605 1104 14-15 0.02646525392396626 1104 16-17 0.05728832656376426 1104 18-19 0.04192480942821675 1104 20-21 0.09871370231531529 1104 22-23 0.01979822223476191 1104 24-25 0.08933462814378856 1104 26-27 0.13875674648868852 1104 28-29 0.03865595566232827 1104 30-31 0.06391267128498157 1104 32-33 0.02987950209973178 1104 34-35 0.04290743480407144 1104 36-37 -0.011471295380104607 1104 38-39 0.0630601710349552 1104 40-41 0.051359334286864566 1104 42-43 -0.03379669713579858 1104 44-45 -0.0737913605090057 1104 46-47 -0.023148874499085537 1104 48-49 0.028524434388671693 1104 50-51 -0.09943240880729576 1104 52-53 -0.044351216829142004 1104 54-55 0.15699705330785463 1104 56-57 0.07824926058751913 1104 58-59 0.10092200062130274 1104 60-61 0.0835532306005291 1104 62-63 0.1261694001594691 1104 64-65 0.08224766060282462 1104 66-67 0.02745442245379337 1104 68-69 0.11713812914607757 1104 70-71 0.16134837284609915 1104 72-73 0.09594573534383244 1104 74-75 0.07864351801155323 1104 76-77 0.12209626918738081 1104 78-79 0.05590570250582161 1104 80-81 0.04226216949937012 1104 82-83 0.0055827075875463095 1104 84-85 0.035765265969356363 1104 86-87 -0.05024013113519388 1104 88-89 -0.13261356883830544 1104 90-91 -0.0306560753122902 1104 92-93 -0.09721505923942786 1104 94-95 -0.03936382737445854 1104 96-97 -0.12408758487883986 1104 98-99 -0.11274613007799772 1104 100-101 -0.15662508900656746 1105 1 0.3162564621312498 1105 2 0.3055844411616171 1105 3 0.23156109875439412 1105 4 0.3158971071484018 1105 5 0.2530078004506038 1105 6 0.34812423064133924 1105 7 0.3667974191413421 1105 8 0.2990395334571332 1105 9 0.3992682176322546 1105 10-11 0.39714514951912605 1105 12-13 0.37004945801788836 1105 14-15 0.45705778999826663 1105 16-17 0.43074335378611295 1105 18-19 0.42859521278042934 1105 20-21 0.4185381622738831 1105 22-23 0.3728688004071543 1105 24-25 0.38060160655786035 1105 26-27 0.45991361925735674 1105 28-29 0.40538573808596823 1105 30-31 0.4101341809146035 1105 32-33 0.38851312735504706 1105 34-35 0.3797441506827113 1105 36-37 0.391348791164674 1105 38-39 0.38332284201678846 1105 40-41 0.4072660208866452 1105 42-43 0.4582819411236372 1105 44-45 0.48369456316692094 1105 46-47 0.49936385124559024 1105 48-49 0.3283329455023889 1105 50-51 0.3003959462915944 1105 52-53 0.4005550933786495 1105 54-55 0.4751059927013159 1105 56-57 0.5511950723337051 1105 58-59 0.5150006705717018 1105 60-61 0.5220866567289377 1105 62-63 0.5680235216146841 1105 64-65 0.4958577902949699 1105 66-67 0.5018465828045819 1105 68-69 0.5201008095175936 1105 70-71 0.5365495284023325 1105 72-73 0.5404296490580265 1105 74-75 0.5052211906562292 1105 76-77 0.5271504288450473 1105 78-79 0.5478110927474482 1105 80-81 0.6018682459677898 1105 82-83 0.5362217649945329 1105 84-85 0.5147333187006318 1105 86-87 0.528256772404653 1105 88-89 0.5326415349993958 1105 90-91 0.5215264566700455 1105 92-93 0.561474430609838 1105 94-95 0.4960649899419778 1105 96-97 0.4503280094482065 1105 98-99 0.46737459451341934 1105 100-101 0.584641747045783 1106 1 0.25589774564468826 1106 2 0.31564455492659604 1106 3 0.3113148939345507 1106 4 0.2917760154068034 1106 5 0.3111865140616601 1106 6 0.30473754215321236 1106 7 0.34524140565362416 1106 8 0.3243427526205096 1106 9 0.3464696053142191 1106 10-11 0.3525805778957505 1106 12-13 0.4111699627684118 1106 14-15 0.3924763204970674 1106 16-17 0.43226876908876477 1106 18-19 0.3818347846230452 1106 20-21 0.37401517529607275 1106 22-23 0.4205857534326114 1106 24-25 0.4725471147872895 1106 26-27 0.4425793289206368 1106 28-29 0.4703765003651412 1106 30-31 0.44190570834938114 1106 32-33 0.47099808970744306 1106 34-35 0.4417850714095124 1106 36-37 0.4582613570209446 1106 38-39 0.4248059523934913 1106 40-41 0.4977316306656334 1106 42-43 0.5620754688176248 1106 44-45 0.5073684912356811 1106 46-47 0.47376964879119043 1106 48-49 0.3711313165826695 1106 50-51 0.353883162890682 1106 52-53 0.5218901012494541 1106 54-55 0.6103960586334978 1106 56-57 0.6515011228418999 1106 58-59 0.6012042988936557 1106 60-61 0.6210073689938795 1106 62-63 0.6418009868687236 1106 64-65 0.727533886819657 1106 66-67 0.7299825230577568 1106 68-69 0.7752431137351294 1106 70-71 0.7411353894917667 1106 72-73 0.6604152078731147 1106 74-75 0.7239308514044751 1106 76-77 0.7936856464210678 1106 78-79 0.7773443551117332 1106 80-81 0.7381496492799577 1106 82-83 0.7551499440829552 1106 84-85 0.7857407815607047 1106 86-87 0.7198853884704555 1106 88-89 0.8251665006885567 1106 90-91 0.7672435736622738 1106 92-93 0.7145649718209732 1106 94-95 0.7453909207818867 1106 96-97 0.77644687749574 1106 98-99 0.7375774373927122 1106 100-101 0.8054340105353361 1107 1 0.23626587381393804 1107 2 0.19270752520401402 1107 3 0.22620025037952374 1107 4 0.2328801281596995 1107 5 0.2456473683048941 1107 6 0.2756527082928031 1107 7 0.3120499031860149 1107 8 0.27709264742873785 1107 9 0.38546914287805834 1107 10-11 0.30434836579773616 1107 12-13 0.35366019129460824 1107 14-15 0.43978295069624807 1107 16-17 0.419045601142912 1107 18-19 0.3743452664338136 1107 20-21 0.44065329763919436 1107 22-23 0.46276858193855475 1107 24-25 0.40500626814716156 1107 26-27 0.46962989660895005 1107 28-29 0.4917550160613189 1107 30-31 0.5139889975854572 1107 32-33 0.5232307746588845 1107 34-35 0.5295894236237189 1107 36-37 0.47940329502853274 1107 38-39 0.5860596299536454 1107 40-41 0.5580619003657077 1107 42-43 0.5068750850997787 1107 44-45 0.6120564887452034 1107 46-47 0.5090739545806073 1107 48-49 0.45246160787215217 1107 50-51 0.5728481785309185 1107 52-53 0.5750293603581866 1107 54-55 0.5964215338383596 1107 56-57 0.5942978908313563 1107 58-59 0.632321874079544 1107 60-61 0.7095371556648118 1107 62-63 0.6541231516315662 1107 64-65 0.6037523487264949 1107 66-67 0.6282175357404114 1107 68-69 0.6480968581469853 1107 70-71 0.7360567443949861 1107 72-73 0.6456086150402314 1107 74-75 0.6231435927208935 1107 76-77 0.6250024028095993 1107 78-79 0.6313301758498504 1107 80-81 0.5547360249716711 1107 82-83 0.5588139391478855 1107 84-85 0.5874500440376877 1107 86-87 0.5154622160557487 1107 88-89 0.5919839963257552 1107 90-91 0.6094550874016704 1107 92-93 0.6026551950328525 1107 94-95 0.6461653767928581 1107 96-97 0.7032846913367301 1107 98-99 0.7211986371088202 1107 100-101 0.6909386826804784 1108 1 0.07535909802237839 1108 2 0.08099517122113653 1108 3 0.06395557172493227 1108 4 0.03438619136827725 1108 5 0.0910333103816825 1108 6 0.12458618580649983 1108 7 0.17666200909717134 1108 8 0.09860407374112867 1108 9 0.14995860722398646 1108 10-11 0.14904673768447907 1108 12-13 0.1304791749920753 1108 14-15 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-0.7679997265871883 2316 100-101 -0.7788193397574084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45036.0 3 9265.0 4 7527.0 5 6788.0 6 7296.0 7 7915.0 8 8818.0 9 10896.0 10 13637.0 11 16803.0 12 21609.0 13 26563.0 14 29872.0 15 35470.0 16 43859.0 17 55881.0 18 44323.0 19 25829.0 20 22527.0 21 22661.0 22 23297.0 23 25665.0 24 29458.0 25 34717.0 26 42097.0 27 50787.0 28 61676.0 29 76602.0 30 95023.0 31 120284.0 32 156402.0 33 210321.0 34 296900.0 35 449943.0 36 712653.0 37 1133001.0 38 1291926.0 39 353689.0 40 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.724445933468466 20.914019822780467 15.115434415354168 21.246099828396893 2 15.547275153587004 21.301662839542836 40.808496126047885 22.342565880822278 3 17.69249962502035 27.097557976119308 31.764366341257784 23.445576057602562 4 12.178806950147978 18.338569219529212 40.053631919540386 29.428991910782422 5 12.077602378797819 38.14384604065651 35.940184580038824 13.838367000506837 6 27.967897882879665 39.409724258609785 18.859016342165717 13.763361516344833 7 24.968940523078732 34.24649646790082 23.649470322849734 17.135092686170708 8 24.686158189530083 35.40034679477849 21.322422999705527 18.591072015985898 9 24.817421082115548 17.172637582003226 22.887412807147754 35.122528528733476 10-11 22.83403896471458 27.62993606886382 29.674355079279675 19.86166988714193 12-13 24.116614901644088 25.677021092973014 29.71670113323812 20.48966287214478 14-15 21.49535591913384 27.41103765477937 28.1430705145538 22.95053591153299 16-17 19.87490148787629 30.65005961155322 28.624501952292807 20.850536948277682 18-19 19.790021778171873 28.68493305760148 32.003303024214915 19.521742140011725 20-21 20.91328478941828 27.81934370373148 31.88754817390757 19.379823332942667 22-23 20.536438217355567 27.83837848553291 32.03663427629107 19.588549020820455 24-25 20.206935460494847 27.986546831370738 31.874579743986725 19.931937964147693 26-27 20.00060066266598 28.108044996742116 31.85437626735602 20.036978073235883 28-29 20.01459000486926 27.9163926968229 32.12647988143178 19.942537416876068 30-31 20.39609930391933 28.322519243535293 31.585068070432463 19.696313382112915 32-33 19.925323985251396 28.22004660297972 31.910814578287816 19.94381483348107 34-35 20.31325777386575 28.02427577588491 31.6607462008345 20.001720249414838 36-37 20.698097371136758 28.084599036556785 31.212955000516253 20.004348591790208 38-39 20.41058957942478 28.3980018762843 31.318620193532027 19.872788350758892 40-41 20.221313986708438 28.08513659430875 31.517001514831634 20.176547904151178 42-43 20.239668109197538 28.287659310242 31.159315528978286 20.31335705158217 44-45 20.411431731846932 28.54201672495529 30.80303227009807 20.243519273099714 46-47 20.788543365966103 28.67882915175149 30.27294809051508 20.259679391767328 48-49 20.702448421774726 28.617131310546323 30.21854878982884 20.46187147785011 50-51 20.734361404466586 28.738851662600446 29.820123095559005 20.706663837373963 52-53 20.715551490998134 28.856720588801167 29.726012947319656 20.701714972881046 54-55 20.75118683371674 29.332351828840025 29.619729804277295 20.296731533165943 56-57 20.736954021124504 28.8726029667431 29.664356474794413 20.72608653733798 58-59 20.662240392082253 28.565874771861022 29.868014175429153 20.903870660627568 60-61 20.86986222677913 28.761151706751043 29.686147035169657 20.682839031300173 62-63 20.670057725603236 28.830982117191905 29.770301592174587 20.728658565030273 64-65 20.783541858781803 28.803026665299463 29.704262499131428 20.70916897678731 66-67 20.633987638958644 28.7917933319756 29.632778120764865 20.941440908300894 68-69 20.99094990097414 29.093299162769654 29.304412752970443 20.611338183285763 70-71 21.037369333420173 28.743453175952173 29.32335630654762 20.895821184080035 72-73 20.657836618307634 28.885290437198552 29.496030068002028 20.960842876491785 74-75 20.724517906091304 29.030768726341584 29.38951520887293 20.855198158694183 76-77 20.902688095268353 29.25460168465925 29.11818997811601 20.724520241956387 78-79 20.894340145409558 29.095720806679253 29.36964221115869 20.64029683675249 80-81 20.87427447862079 29.06298637280811 29.25100831409244 20.811730834478663 82-83 20.73299081641966 29.053444884610048 29.526207449436274 20.687356849534012 84-85 20.617728819610967 29.009855406584506 29.368439825876884 21.00397594792764 86-87 20.83413232938554 29.132252077671122 29.2237685967627 20.809846996180635 88-89 20.69634274024756 29.08660162269026 29.293567538091033 20.92348809897115 90-91 20.59410193403946 28.913986789836414 29.37910773905457 21.11280353706955 92-93 20.784195739897047 29.056380495885918 29.182314784719278 20.97710897949776 94-95 20.75494716736427 28.82414397872869 29.38349870244205 21.03741015146499 96-97 20.751425784901432 28.952727846576092 29.238240251242388 21.057606117280095 98-99 20.669596979109418 28.88916336810284 29.188719911388635 21.252519741399112 100-101 20.77522003069557 28.94279073061628 28.958253151645742 21.323736087042413 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3565.0 1 2371.0 2 1911.0 3 2541.5 4 1940.5 5 1568.5 6 2431.5 7 4570.5 8 7419.0 9 9150.5 10 8276.0 11 6828.5 12 6828.0 13 7374.5 14 7805.0 15 8244.5 16 8789.0 17 9366.0 18 10120.5 19 11327.0 20 13043.0 21 15451.0 22 19600.5 23 26375.0 24 34019.5 25 42802.0 26 55630.0 27 71405.5 28 91163.0 29 109942.0 30 122237.5 31 135712.0 32 151909.0 33 166490.5 34 178470.5 35 189228.0 36 196222.0 37 201100.0 38 207651.0 39 210770.0 40 212651.5 41 209331.5 42 201904.5 43 197019.5 44 189643.0 45 182396.0 46 176907.0 47 171077.5 48 164611.5 49 159984.5 50 155715.0 51 145134.5 52 129560.5 53 117629.0 54 110499.0 55 104991.5 56 98573.5 57 89113.5 58 79312.5 59 70558.0 60 60768.5 61 49996.0 62 40254.0 63 31924.5 64 25498.5 65 20704.0 66 16542.0 67 12893.0 68 9967.0 69 7568.0 70 5689.0 71 4306.0 72 3374.5 73 2574.0 74 1933.0 75 1475.0 76 1110.5 77 834.0 78 640.0 79 497.5 80 378.0 81 288.5 82 236.0 83 207.5 84 194.0 85 154.5 86 119.0 87 96.0 88 81.0 89 73.5 90 63.5 91 52.5 92 45.5 93 44.5 94 28.0 95 24.5 96 28.0 97 14.0 98 10.0 99 8.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014749826067563088 2 0.004336093446367944 3 0.003785196328181853 4 0.005544512931421306 5 0.0025056933440077054 6 0.006948412039056828 7 0.0013861282328553265 8 0.004016217700324407 9 0.010253794748173376 10-11 0.007597048968534 12-13 0.008476707270153726 14-15 0.0032609555221660563 16-17 0.006033211987876709 18-19 0.003918477889033327 20-21 8.263456772791369E-4 22-23 8.974291763999229E-4 24-25 0.007223860598149874 26-27 0.0011817631728830668 28-29 7.996893651088422E-4 30-31 6.308660546969755E-4 32-33 0.00136835735807513 34-35 0.0016704622293384705 36-37 0.0013505864832949333 38-39 0.0018837127267008283 40-41 0.002790027340490849 42-43 0.002727829278760162 44-45 0.007881382965017145 46-47 0.013959022139844346 48-49 0.005242408060157966 50-51 0.005988784800926218 52-53 0.0136746881433612 54-55 0.007979122776308224 56-57 0.005464543994910422 58-59 0.007223860598149874 60-61 0.001341701045904835 62-63 0.0023546409083760353 64-65 0.00238129722054633 66-67 0.00268340209180967 68-69 0.0021236195362334806 70-71 0.0022124739101344634 72-73 0.0013061592963444424 74-75 0.001626035042387979 76-77 0.008325654834522057 78-79 0.006903984852106338 80-81 0.012688404593060296 82-83 0.015878276616105566 84-85 0.00846782183276363 86-87 0.005899930427025236 88-89 0.003047705024803698 90-91 0.00273671471615026 92-93 7.819184903286456E-4 94-95 0.0013505864832949333 96-97 0.0019370253510414177 98-99 0.00653079648172221 100-101 0.011382245296715852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5627185.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.119422802923715 #Duplication Level Percentage of deduplicated Percentage of total 1 75.70982440287789 41.73081821597337 2 14.728266154155275 16.236270586097515 3 4.406002106769682 7.285688789808321 4 1.8511689757055236 4.081414618062718 5 0.9121484914436241 2.5138549179465084 6 0.5109593575298256 1.689827091767803 7 0.3460087822438355 1.3350263053415916 8 0.22978024306479858 1.013228349539777 9 0.169588953376512 0.8412880701478753 >10 0.984094777640018 10.054376096700667 >50 0.08876703962544572 3.418683970624101 >100 0.057576152158704756 5.930069575437409 >500 0.004199415861702383 1.5122585803763835 >1k 0.0014859357436337402 1.2474679301517726 >5k 3.2302950945538036E-5 0.10296747223319312 >10k+ 9.69088528366141E-5 1.0067594297910147 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24047 0.4273362258393851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21112 0.37517870835950834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11384 0.20230363849775687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5783 0.10276896885387632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.7770874780196493E-5 0.0 4 0.0 0.0 0.0 3.5541749560392986E-5 0.0 5 0.0 0.0 0.0 5.3312624340589476E-5 0.0 6 0.0 0.0 0.0 5.3312624340589476E-5 0.0 7 0.0 0.0 0.0 7.108349912078597E-5 0.0 8 0.0 1.7770874780196493E-5 0.0 7.108349912078597E-5 0.0 9 0.0 1.7770874780196493E-5 0.0 8.885437390098247E-5 0.0 10-11 1.7770874780196493E-5 1.7770874780196493E-5 0.0 1.0662524868117896E-4 0.0 12-13 1.7770874780196493E-5 1.7770874780196493E-5 0.0 1.2439612346137546E-4 0.0 14-15 1.7770874780196493E-5 1.7770874780196493E-5 0.0 1.7770874780196494E-4 0.0 16-17 1.7770874780196493E-5 1.7770874780196493E-5 0.0 1.7770874780196494E-4 0.0 18-19 1.7770874780196493E-5 1.7770874780196493E-5 0.0 1.8659418519206318E-4 0.0 20-21 1.7770874780196493E-5 1.7770874780196493E-5 0.0 2.576776843128491E-4 0.0 22-23 1.7770874780196493E-5 1.7770874780196493E-5 0.0 5.77553430356386E-4 0.0 24-25 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.0010751379242018879 0.0 26-27 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.002105848661453284 0.0 28-29 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.00491364687672433 0.0 30-31 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.011453328795836638 0.0 32-33 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.02494142275400578 0.0 34-35 1.7770874780196493E-5 1.7770874780196493E-5 0.0 0.04573334624683567 0.0 36-37 1.7770874780196493E-5 3.5541749560392986E-5 0.0 0.07717002373300327 0.0 38-39 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.11873609984388286 0.0 40-41 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.16243468092838603 0.0 42-43 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.20696849312755844 0.0 44-45 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.26006786697078554 0.0 46-47 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.32540248810017797 0.0 48-49 1.7770874780196493E-5 5.3312624340589476E-5 0.0 0.39306509382577615 0.0 50-51 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.4615451597912633 0.0 52-53 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.5289500878325486 0.0 54-55 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.6039342939675877 0.0 56-57 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.6935261591719483 0.0 58-59 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.792900890942807 0.0 60-61 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.8930486557666044 0.0 62-63 1.7770874780196493E-5 8.885437390098247E-5 0.0 0.9931608788408414 0.0 64-65 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.1024873004886102 0.0 66-67 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.2311128921476724 0.0 68-69 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.3749236963064124 0.0 70-71 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.528526252469041 0.0 72-73 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.6917161955755853 0.0 74-75 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 1.8596776185606125 0.0 76-77 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 2.039980914080486 0.0 78-79 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 2.246496249901149 0.0 80-81 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 2.4760426394369475 0.0 82-83 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 2.7138969129324875 0.0 84-85 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 2.9560783944370055 0.0 86-87 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 3.2158441565365274 0.0 88-89 1.7770874780196493E-5 8.885437390098247E-5 1.7770874780196493E-5 3.50546498826678 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12190 0.0 59.075775 1 GTATCAA 23070 0.0 42.68405 1 GTGGTAT 4660 0.0 37.308613 1 TGGTATC 4695 0.0 36.218502 2 ATCAACG 28430 0.0 34.0683 3 TATAACG 405 0.0 34.01152 2 TCAACGC 28730 0.0 33.747124 4 TATCAAC 29720 0.0 32.55557 2 CAACGCA 29920 0.0 32.324085 5 AACGCAG 32095 0.0 30.208366 6 CGCAGAG 34970 0.0 27.482035 8 ACGCAGA 35975 0.0 26.859554 7 GCAGAGT 38930 0.0 24.174011 9 GTACATG 20010 0.0 23.45437 1 ATAACGC 575 0.0 23.131311 3 TACATGG 19045 0.0 22.720634 2 GAGTACT 26305 0.0 21.915047 12-13 ACATGGG 19270 0.0 21.791174 3 CAGAGTA 37170 0.0 21.483473 10-11 AGAGTAC 35115 0.0 20.745337 10-11 >>END_MODULE